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Consumables for KingFisher™ mL Systems (Thermo Scientific™)

Optimize system performance with Consumables for Thermo Scientific™ KingFisher™ mL Systems.

24 Deep-Well Head for KingFisher™ Flex Magnetic Particle Processor (Thermo Scientific™)

This is a magnetic head for the KingFisher™ Flex Magnetic Particle Processor (Cat. Nos. 5400630 and 5400640). This magnetic head can serve as a replacement head for the 24 deep-well format system or a supplemental head for the 96 deep-well format system.

KingFisher™ mL Purification System (Thermo Scientific™)

Automate low-throughput sample preparation in your laboratory workflow using Thermo Scientific™ KingFisher mL Purification System.

KingFisher™ Flex Purification System

Prepare samples efficiently from a variety of materials with Thermo Scientific™ KingFisher™ Flex Purification Systems.

AutoMate Express™ Forensic DNA Extraction System (Applied Biosystems™)

The AutoMate Express Forensic DNA Extraction System is an easy to use, robust bench-top instrument that utilizes the PrepFiler Express and PrepFiler Express BTA chemistries packaged in pre-filled, foil sealed cartridges. We have designed the PrepFiler chemistry specifically to improve the quantity and quality of DNA isolated from forensic samples, thereby increasing the potential to obtain maximum information from downstream Short Tandem Repeat (STR) analysis.

Key Product Features:
• The most variable elution volume options on the market, with 7 elution volumes from 20 µL to 250 µL
• Designed to improve the yield and overall purity of DNA isolated from both routine and challenging forensic samples.
• Provides high quality DNA, free of PCR inhibitors and suitable for downstream applications such as quantitative real-time PCR and short tandem repeat (STR) analysis
• Simple and fast separation of substrate from lysate with the unique PrepFiler LySep Column
• Quick and easy run setup with ready-to-use pre-filled cartridges
• Closed system that minimizes the risk of contamination and transposition errors
• Processes 1 to 13 samples in a single run
• Rapid implementation with pre-validated, pre-programmed instrument protocols
• Integrated solution including novel reagents, consumables and automation to provide time saving, cost effective and reliable DNA extraction
• Improves downstream genotyping (STR) success rate and profile quality

PrepFiler Express Kit Format and Components
The Automate Express System utilizes two different configurations of PrepFiler Chemistry. The PrepFiler Express Forensic DNA Extraction Kit is suitable for the majority of standard sample types encountered in forensic laboratories such as bodily fluids on different substrates including FTA paper, cotton swabs, cotton cloth, denim and many others. The PrepFiler Express BTA Forensic DNA Extraction Kit was designed specifically for challenging samples such as bones, teeth and adhesive based samples such as cigarette butts and tape lifts. Both formats come with all the reagents and plastic components including the PrepFiler LySep Columns needed to perform 52 DNA extractions on the AutoMate Express Instrument.

Streamlined Off-Line Lysis
The PrepFiler Express Kits include the innovative PrepFiler LySep Column, which dramatically streamlines the off-line lysis portion of the extraction method. PrepFiler Lysis buffer or PrepFiler BTA Lysis buffer is added directly into the PrepFiler LySep Column along with the sample for incubation. Next, the PrepFiler LySep Column is quickly centrifuged which permits lysate flow through the burstable membrane while the substrate remains behind in the column. The sample tube is then simply ready to be placed on the AutoMate Express instrument. This eliminates the manual lysate and substrate transfer steps, saving time and minimizing cross contamination and sample transposition events.

KingFisher™ mL Food Protection Purification System (Thermo Scientific™)

Carry out rapid purification and concentration of selected targets from a broad range of pre-enriched food samples with the Thermo Scientific KingFisher mL Food Protection Purification System. Combined with Dynabeads immuno-magnetic separation (IMS) products, the system provides an optimized solution for the purification of microorganisms prior to detection using methods such as PCR, ELISA, and agar plates.

Features of the KingFisher mL Food Protection Purification System include:
• Protocols included for E. Coli 0157, EPEC/VTEC, listeria, and salmonella
• Automated processing of up to 15 samples in ~40 min
• High reproducibility and maximum performance of capture technology
• Simple, easy operation: complete processing through internal program
• Small footprint: uses only 11.8 in (30 cm) of bench depth

The system-of-choice for processing volumes up to 1 mL, this magnetic particle processor enables all purification steps in a single-strip of five tubes, and supports 15 processed samples per run. With the ability to release target molecules into a volume as small as 50 µL, samples such as DNA or RNA can be isolated and concentrated from larger starting volumes simultaneously.

AutoLys M Tubes and Caps (Applied Biosystems™)

AutoLys M Tubes are for the preparation of FFPE samples for nucleic acid extraction. They feature a novel tube-in-tube design that creates cleared lysates from FFPE tissue samples, without the need for deparaffinization or wash steps, while increasing DNA and RNA yields. Just add your FFPE sample to the tube, followed by protease buffer and enzyme from the MagMAX FFPE DNA/RNA Ultra Kit. Once digestion is complete, lift the tubes from the AutoLys M Tube Rack using the AutoLys M TubeLifter or AutoLys M Tube Pliers and spin down the samples using a benchtop centrifuge with plate holder (SBS standard format). The FFPE-associated wax and debris are held in the upper chamber of the tube while the lysate passes through. Nucleic acids can then be purified from the clarified lysate using the MagMAX FFPE DNA/RNA Ultra Kit.

Features of the AutoLys M Tube system include:
• FFPE sample lysis and nucleic acid separation from debris in a single tube
• 2-D barcode on bottom tube
• Elimination of use of organic solvents (xylene, CitriSolv) for deparaffinization and washes
• Reduced processing time
• Increased nucleic acid yield

The AutoLys M Tube system is a unique way to process your FFPE samples that will result in improved nucleic acid yields when used in conjunction with the MagMAX FFPE DNA/RNA Ultra Kit, helping ensure the success of downstream assays and reducing the time to data analysis.

iPrep™ Card: Viral DNA/RNA (Invitrogen™)

The iPrep™ Viral DNA/RNA protocol Card is for use with the iPrep™ PureLink™ Virus Kit. The iPrep™ Protocol Card is a flash, 512 KB memory card. Each iPrep™ Protocol card is pre-programmed with the purification protocol that directs the volume of reagents used and incubation time. The following iPrep™ Protocol Cards are available from Invitrogen:
• iPrep™ Forensic Card for use with the iPrep™ ChargeSwitch® Forensic Kit and the iPRep™ ChageSwitch® gDNA Buccal kit
• iPrep™ Tissue Card for use with the iPrep™ ChargeSwitch® Tissue Kit
• iPrep™ Blood Card for use with the iPrep™ PureLink™ Blood kit
• iPrep™ Viral DNA/RNA Card for use with the iPrep™ PureLink™ Virus Kit

PrepFiler™ 96-Well Processing Plates (Applied Biosystems™)

The PrepFiler 96-well Processing Plate is now available for purchase separately to provide the ordering flexibility to select only the plastics consumables needed for use with the PrepFiler Automated Forensic DNA Extraction Kit. The full PrepFiler Automated Forensic DNA Extraction Kit uses magnetic particles with an optimized multi-component surface chemistry to deliver superior DNA yield from most forensic sample types, including body fluids, and stains and swabs of body fluids.

The PrepFiler 96-well Processing Plate is used for sample processing in 96-well format.

The full PrepFiler Automated Forensic DNA Extraction Kit is also available for purchase, and contains other components required for DNA recovery from both routine and challenging samples, including removal of PCR inhibitors.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

KingFisher™ Flex™ Systems Consumables (Thermo Scientific™)

Optimize performance using consumables specifically designed for use with the KingFisher™ Flex™ System and ideal for magnetic particle processing.

KingFisher™ Presto Purification System (Thermo Scientific™)

Automate the extraction of DNA, RNA and proteins with the Thermo Scientific™ KingFisher™ Presto Purification System.

KingFisher™ Duo Consumables (Thermo Scientific™)

Carry out magnetic particle processing with Thermo Scientific™ KingFisher™ Duo and KingFisher Duo Prime Consumables, specially designed combs and microplates optimized for performance.

BenchPro™ 2100 Waste Tray (Invitrogen™)

The BenchPro® 2100 Waste Tray is used with the BenchPro® 2100 Plasmid Processing Station (Cat# MC1001) and supports the disposable Reagent Tray inside the instrument drawer.

Deep Well Plate (96 well)

96-well, deep-well plastic plates for nucleic acid purification on the KingFisher™Flex system.

For veterinary use only.

Regulatory requirements vary by country; products may not be available in your geographic area.

Biological hazard safety

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety glasses, or goggles. Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially biohazardous materials. Follow all applicable local, state/provincial, and/or national regulations. The following references provide general guidelines when handling biological samples in laboratory environment.

U.S. Department of Health and Human Services, Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC) 21-1112, Revised December 2009

World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/CDS/CSR/LYO/2004.11

iPrep™ Card: gDNA Tissue (Invitrogen™)

The iPrep™ Protocol Card is a flash, 512 KB memory card. Each iPrep™ Protocol card is pre-programmed with the purification protocol that directs the volume of reagents used and incubation time.
The following iPrep™ Protocol Cards are available from Invitrogen:
- iPrep™ Forensic Card for use with the iPrep™ ChargeSwitch® Forensic Kit and the iPRep™ ChageSwitch® gDNA Buccal kit
-iPrep™ Tissue Card for use with the iPrep ChargeSwitch® Tissue Kit
- iPrep™ Blood Card for use with the iPrep PureLink Blood kit