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Neon™ Transfection System 100 µL Kit (Invitrogen™)

The Neon® Transfection System 100 µL Kit is designed specifically for use with the Neon® Transfection System. Use this kit for transfection volumes of 100 µL, containing 5 × 105–2 × 106 adherent cells or 1 × 106–5 × 106 suspension cells. Cells that have been transfected using the included 100 µL Neon® Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol.

Neon™ Transfection System 10 µL Kit (Invitrogen™)

The Neon® Transfection System 10 µL Kit is designed specifically for use with the Neon® Transfection System. Use this kit for transfection volumes of 10 µL, containing 5 × 104–2 × 105 adherent cells or 1 × 105–5 × 105 suspension cells. Cells that have been transfected using the included 10 µL Neon® Tips are ready to be washed and plated into a multi-well culture dish.

Neon™ Transfection System Pipette Station (Invitrogen™)

The Neon® Transfection System Pipette Station is designed specifically for use with the Neon® Transfection System device (MPK5000). Each device (MPK5000) already comes with a pipette station. The pipette station is also sold separately for those labs needing and additional pipette station or replacement for their existing pipette station.

 

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Neon™ Transfection System Starter Pack (Invitrogen™)

The Neon® Transfection System Starter Pack contains a Neon® Transfection device and two 192 reaction kits (10 µL and 100 µL sample sizes) to get you started in a fast, convenient, and cost effective manner. The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:

Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
Simple—easy to use, with a single reagent kit for all cell types
Versatile—open system allows electroporation parameters to be optimized freely

One-of-a-kind electroporation
Unlike standard cuvette-based electroporation chambers, the Neon® system uses a patented, biologically compatible pipette tip chamber that generates a more uniform electric field. This design allows better maintenance of physiological conditions, resulting in very high cell survival compared to conventional electroporation (see figure).

Plug-n-play out of the box
The transfection process couldn’t be simpler. Draw up the cells and plasmid mix into the Neon® pipette tip, plug into the pipette holder, and press start. Then just pipette the transfected cells into your culture vessel. No more filling and pulling sample from the cuvette and capping and de-capping. The Neon® system uses a simple 3-step transfection procedure. The transfection occurs in the Neon® pipette tip.

Simplified transfection kit for all cell types
Avoid the hassle of determining which proprietary buffer kit will work with your favorite cell type. We have simplified your work with just one transfection kit that is compatible with all cell types. Start your transfection with our optimized protocols for popular cell types, or follow our standard simple optimization procedure for all new cell types.

Perform more transfections with limited or precious cells
Your cells may be limited when you are required to perform neuronal preps or when enriching for homogeneous cell populations via FACS or Dynabeads® enrichment. In these instances, reducing the number of cells required per transfection can allow you to perform more complex experiments with your precious cells.

Neon™ Transfection System (Invitrogen™)

The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:

Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
Simple—easy to use, with a single reagent kit for all cell types
Versatile—open system allows electroporation parameters to be optimized freely

One-of-a-kind electroporation
Unlike standard cuvette-based electroporation chambers, the Neon® system uses a patented, biologically compatible pipette tip chamber that generates a more uniform electric field. Neon® Tips are components of the Neon® Transfection System Kits, available in 100 µL and 10 µL formats for large- and small-scale transfections. This design allows better maintenance of physiological conditions, resulting in very high cell survival compared to conventional electroporation (see figure).

Plug-n-play out of the box
The transfection process couldn’t be simpler. Draw up the cells and plasmid mix into the Neon® pipette tip, plug into the pipette holder, and press start. Then just pipette the transfected cells into your culture vessel. No more filling and pulling sample from the cuvette and capping and de-capping. The Neon® system uses a simple 3-step transfection procedure. The transfection occurs in the Neon® pipette tip.

Perform more transfections with limited or precious cells
Your cells may be limited when you are required to perform neuronal preps or when enriching for homogeneous cell populations via FACS or Dynabeads® enrichment. In these instances, reducing the number of cells required per transfection can allow you to perform more complex experiments with your precious cells.

Neon™ Transfection Tubes (Invitrogen™)

The Neon® Transfection Tubes are designed specifically for use with the Neon® Transfection System device (MPK5000). Each Neon® Transfection System Kit (MPK1096 and MPK10096) already comes with 20 electroporation tubes. The additional pack of 100 tubes is offered for those labs wishing to use single tubes per reaction depending on their experimental set-up.

 

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Neon™ Transfection System Pipette (Invitrogen™)

The Neon® Transfection System Pipette is designed specifically for use with the Neon® Transfection System device (MPK5000). Each device (MPK5000) already comes with a pipette. The pipette is also sold separately for those labs needing and additional pipette or replacement for their existing pipette.

 

For more information on the product, please click here