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Attune™ NxT Acoustic Focusing Cytometer, blue/violet, refurbished (Invitrogen™)

This is a refurbished Attune NxT Acoustic Focusing Cytometer, which is an easy-to-use instrument designed to detect cell size and granularity, as well as fluorescence expression of labeled antibodies, proteins, and functional reagents. The innovative design of the instrument results in precise data and acquisition speeds up to 10X faster than other flow cytometers. The design also eliminates lengthy sample preparation procedures, reduces the time to analyze a file, allows small or limited samples to be analyzed, and minimizes variability in measurements, leading to better resolution of populations. It is ideal for immunophenotyping and signaling studies, cell cycle analysis, detection of rare events, stem cell analysis, cancer and apoptosis studies, and microbiological assays.

With the Attune NxT Acoustic Focusing Cytometer you get:
• A flexible platform that provides highly sensitive and accurate measurement of cell sizeand granularity, as well as fluorescence detection
• State of the art optics for long-term reliability
• Acoustic focusing for high-precision data
• An intuitive software user interface that reduces time to proficiency
• Improved workflow to reduce sample preparation time before analysis
• Faster data analysis that leads to reduced acquisition times

Flexible design that accommodates changing needs, budget, or laboratory space
The instrument's modular design accommodates the most common fluorophores and fluorescent proteins used in flow cytometry enabling alignment with previous experimental results and accommodating future needs through simple upgrades. The small footprint of the instrument requires minimal bench space, even allowing use in a cell culture hood.

Acoustic focusing improves efficiencies compared to traditional flow cytometers
Traditional flow cytometers rely on a large volume of fluid to focus cells prior to analysis, resulting in extensive sample preparation prior to analysis and introducing the possibility of error due to artifacts created during the sample preparation process. With the Attune NxT Acoustic Focusing Cytometer, blood samples can be run without being manipulated using the No-Wash No-Lyse blood cell assay. The instrument also offers flexibility in the sample concentration that can be analyzed.

Acoustic focusing enables:
• Elimination of concentration steps
• Effective background fluorescence reduction by a simple dilution step, eliminating the need to wash fluorescence samples before analysis
• No-lyse/no-wash assays
• Analysis of samples that have low cell numbers, including CSF, stem cells, mouse bone marrow, mouse blood, and thin needle aspirates
• Acquisition of a high volume of samples in minimal time

Learn more about the Attune NxT cytometer and see all available configurations >
Learn more about how acoustic focusing works >

Attune™ NxT Violet 6 Conversion Kit for Yellow Laser (Invitrogen™)

The Attune NxT Violet 6 Conversion kits are designed to reconfigure Attune NxT four-channel violet instruments to allow detection of six channels of violet. A conversion kit is required for each original and any upgraded lasers of the instrument. The conversion is carried out by a Field Service Engineer.

Attune™ NxT Yellow Laser Upgrade Kit

The Attune® NxT Yellow Laser Upgrade Kit is designed to expand the 561 nm excitation capabilities of the Attune® NxT Acoustic Focusing Cytometer. Given the large array of labels, dyes, and applications to choose from, the ability to upgrade your instrument is key. The Yellow Laser upgrade kit enables the detection of many red-fluorescent proteins and provides a better excitation source for many PE and PE-Tandem dyes.

Expanded capabilities
A great way to stretch your research dollars is through upgrades, thereby expanding the capabilities of your instrument while minimizing expenditure on new instruments. Through the upgrade path, the Attune® NxT cytometer allows you to configure your instrument with different laser configurations to meet your ever-changing research needs.

Simple installation
The Attune® NxT cytometer was designed to minimize the time it takes for a qualified service engineer to add additional capabilities. This Yellow Laser upgrade kit comes with all of the optical parts needed to upgrade the instrument in typically less than 4 hours.

Attune™ NxT Emission Filter, 530/30BP

This is a replacement 530/30 bandpass filter used in the optics path of the Attune® NxT Acoustic Focusing Cytometer.

Attune™ NxT Acoustic Focusing Cytometer, blue/red/yellow (Invitrogen™)

The 2019 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.

• Free Attune NxT software with researcher-inspired upgrades
• New green laser option for expanded utility and fluorochrome options
• Simple push-button sample recovery function

Freakishly fast flow
The innovative combination of acoustic focusing technology coupled with traditional hydrodynamic focusing results in acquisition speeds up to 10X faster than other flow cytometers. This technology reduces the time needed to obtain results.

• True 35,000 event/sec (+10% coincidence rates)
• Maximum electronic speed of 65,000 events/sec
• Analyze small or limited samples faster than ever
• Efficiently run traditionally long assays

The Attune NxT flow cytometer is ideal for immunophenotyping and signaling studies, cell cycle analysis, detection of rare events, stem cell analysis, cancer and apoptosis studies, microbiological assays, and more.

Fit for your lab today and in the future
The instrument’s modular design provides the flexibility to meet future capability needs with a variety of upgrade options. Purchase what you need now and easily upgrade later. The small footprint of the instrument requires minimal bench space, even allowing use in a cell culture hood.

• Flexibility—1 to 4 lasers, 14 colors, and 16 parameters
• Field upgradeable—choose to add additional lasers when you decide
• Customizable band pass filters for expanded fluorochrome choices

Tune into your data
The Attune NxT Acoustic Focusing Cytometer easily runs most samples including cardiomyocytes. The system is designed with a clog-resistant syringe-driven system to push the sample with a force of up to 75 psi.

• Flexibility in sample concentrations and sample types
• Elimination of time-consuming concentration steps with No-Wash, No-Lyse Filter Kit
• Analysis of dilute samples, including CSF, stem cells, mouse bone marrow, mouse blood, and thin needle aspirates
• Acquisition of a high volume of samples in minimal time

Learn what’s new with the 2019 Attune NxT cytometer and see all available configurations >
Learn more about how acoustic focusing works >

Attune™ NxT Acoustic Focusing Cytometer, blue/red

The 2019 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.

• Free Attune NxT software with researcher-inspired upgrades
• New green laser option for expanded utility and fluorochrome options
• Simple push-button sample recovery function

Freakishly fast flow
The innovative combination of acoustic focusing technology coupled with traditional hydrodynamic focusing results in acquisition speeds up to 10X faster than other flow cytometers. This technology reduces the time needed to obtain results.

• True 35,000 event/sec (+10% coincidence rates)
• Maximum electronic speed of 65,000 events/sec
• Analyze small or limited samples faster than ever
• Efficiently run traditionally long assays

The Attune NxT flow cytometer is ideal for immunophenotyping and signaling studies, cell cycle analysis, detection of rare events, stem cell analysis, cancer and apoptosis studies, microbiological assays, and more.

Fit for your lab today and in the future
The instrument’s modular design provides the flexibility to meet future capability needs with a variety of upgrade options. Purchase what you need now and easily upgrade later. The small footprint of the instrument requires minimal bench space, even allowing use in a cell culture hood.

• Flexibility—1 to 4 lasers, 14 colors, and 16 parameters
• Field upgradeable—choose to add additional lasers when you decide
• Customizable band pass filters for expanded fluorochrome choices

Tune into your data
The Attune NxT Acoustic Focusing Cytometer easily runs most samples including cardiomyocytes. The system is designed with a clog-resistant syringe-driven system to push the sample with a force of up to 75 psi.

• Flexibility in sample concentrations and sample types
• Elimination of time-consuming concentration steps with No-Wash, No-Lyse Filter Kit
• Analysis of dilute samples, including CSF, stem cells, mouse bone marrow, mouse blood, and thin needle aspirates
• Acquisition of a high volume of samples in minimal time

Learn what’s new with the 2019 Attune NxT cytometer and see all available configurations >
Learn more about how acoustic focusing works >

Attune™ Auto Sampler Focusing Fluid Bottle (Applied Biosystems™)

This is a replacement bottle for focusing fluid for the Attune® Auto Sampler.

Attune™ NxT Custom Filter Holder Kit

The Attune® NxT Custom Filter Holder Kit allows users of the Attune® NxT acoustic focusing cytometer to make their own custom emission or dichroic filters in order to customize the optical configuration of the instrument. Each kit contains sufficient parts to make 2 emission filters and 2 dichroic filters. The kit includes instructions for attaching the filter to the filter blade, but does not include the glass filter.

Attune™ NxT Automation Bundle, stacks/hotel (Invitrogen™)

The Attune NxT Automation Bundle consists of an Orbitor RS2 Microplate Mover and a barcode reader for quick and easy plate identification. This Orbitor RS2 Microplate Mover is an automation robot designed for use with the Attune NxT Acoustic Focusing Cytometer that enables flow cytometry in automated environments for increased sample throughput. It is offered in three different configurations: one with sequential access stacks for plate storage and handling (Cat. No. A33007), one with random access hotels (24 plates/3 hotels) (Cat. A33008), and one with two sequential access stacks and one random access hotel (this page).

Extensive vertical reach allows the Orbitor RS2 Microplate Mover to access virtually any microplate instrument location, including stacked instruments, and its telescoping arm can reach a variety of distances. The ingenious arm travels through the base, resulting for rapid and efficient movements. This robot has a full 360°workspace with unlimited base rotation; this eliminates the need for hard stops and reverse movements.

Advanced closed-loop motion control provides collision detection and position monitoring, allowing the Orbitor RS2 Microplate Mover to quickly resume without the need to re-home. With a wide range of grip-control functionality, this robot combines performance with innovation. From variable force control to plate detection, the feature set is designed to impress. The result is remarkable speed, precise motion, and consistent, accurate plate placement.

The Orbitor RS2 Microplate Mover is powered by Thermo Scientific Momentum Integration Software with features such as Momentum scheduling software, error detection and recovery, and a dynamic scheduler that allows changes while running.

Attune™ NxT Violet 6 Conversion Kit for Violet Laser (Invitrogen™)

The Attune NxT Violet 6 Conversion kits are designed to reconfigure Attune NxT four-channel violet instruments to allow detection of six channels of violet. A conversion kit is required for each original and any upgraded lasers of the instrument. The conversion is carried out by a Field Service Engineer.

CytKick™ Max Extended Cooling Adaptor (Invitrogen™)

The CytKick Max Extended Cooling Adaptor is designed for use with the CytKick Max Autosampler and the Attune Acoustic Focusing Cytometer. This cooling block maintains samples at 2-15°C for 95-100 minutes while they are being analyzed on the instrument. The cooling adaptor is brought to temperature by placing it in a refrigerator overnight or in a freezer for a minimum of two hours prior to use. If this longer cooling time is not required, please see the CytKick Max Universal Cooling Adaptor, which cools samples for ~65 minutes.

Attune™ NxT Emission Filter, 585/16BP

This is a replacement 585/16 bandpass filter used in the optics path of the Attune® NxT Acoustic Focusing Cytometer.

Attune™ NxT Acoustic Focusing Cytometer, blue/red/yellow, refurbished (Invitrogen™)

This is a refurbished Attune NxT Acoustic Focusing Cytometer, which is an easy-to-use instrument designed to detect cell size and granularity, as well as fluorescence expression of labeled antibodies, proteins, and functional reagents. The innovative design of the instrument results in precise data and acquisition speeds up to 10X faster than other flow cytometers. The design also eliminates lengthy sample preparation procedures, reduces the time to analyze a file, allows small or limited samples to be analyzed, and minimizes variability in measurements, leading to better resolution of populations. It is ideal for immunophenotyping and signaling studies, cell cycle analysis, detection of rare events, stem cell analysis, cancer and apoptosis studies, and microbiological assays.

With the Attune NxT Acoustic Focusing Cytometer you get:
• A flexible platform that provides highly sensitive and accurate measurement of cell sizeand granularity, as well as fluorescence detection
• State of the art optics for long-term reliability
• Acoustic focusing for high-precision data
• An intuitive software user interface that reduces time to proficiency
• Improved workflow to reduce sample preparation time before analysis
• Faster data analysis that leads to reduced acquisition times

Flexible design that accommodates changing needs, budget, or laboratory space
The instrument's modular design accommodates the most common fluorophores and fluorescent proteins used in flow cytometry enabling alignment with previous experimental results and accommodating future needs through simple upgrades. The small footprint of the instrument requires minimal bench space, even allowing use in a cell culture hood.

Acoustic focusing improves efficiencies compared to traditional flow cytometers
Traditional flow cytometers rely on a large volume of fluid to focus cells prior to analysis, resulting in extensive sample preparation prior to analysis and introducing the possibility of error due to artifacts created during the sample preparation process. With the Attune NxT Acoustic Focusing Cytometer, blood samples can be run without being manipulated using the No-Wash No-Lyse blood cell assay. The instrument also offers flexibility in the sample concentration that can be analyzed.

Acoustic focusing enables:
• Elimination of concentration steps
• Effective background fluorescence reduction by a simple dilution step, eliminating the need to wash fluorescence samples before analysis
• No-lyse/no-wash assays
• Analysis of samples that have low cell numbers, including CSF, stem cells, mouse bone marrow, mouse blood, and thin needle aspirates
• Acquisition of a high volume of samples in minimal time

Learn more about the Attune NxT cytometer and see all available configurations >
Learn more about how acoustic focusing works >

Attune™ NxT Acoustic Focusing Cytometer, blue/red, refurbished (Invitrogen™)

This is a refurbished Attune NxT Acoustic Focusing Cytometer, which is an easy-to-use instrument designed to detect cell size and granularity, as well as fluorescence expression of labeled antibodies, proteins, and functional reagents. The innovative design of the instrument results in precise data and acquisition speeds up to 10X faster than other flow cytometers. The design also eliminates lengthy sample preparation procedures, reduces the time to analyze a file, allows small or limited samples to be analyzed, and minimizes variability in measurements, leading to better resolution of populations. It is ideal for immunophenotyping and signaling studies, cell cycle analysis, detection of rare events, stem cell analysis, cancer and apoptosis studies, and microbiological assays.

With the Attune NxT Acoustic Focusing Cytometer you get:
• A flexible platform that provides highly sensitive and accurate measurement of cell sizeand granularity, as well as fluorescence detection
• State of the art optics for long-term reliability
• Acoustic focusing for high-precision data
• An intuitive software user interface that reduces time to proficiency
• Improved workflow to reduce sample preparation time before analysis
• Faster data analysis that leads to reduced acquisition times

Flexible design that accommodates changing needs, budget, or laboratory space
The instrument's modular design accommodates the most common fluorophores and fluorescent proteins used in flow cytometry enabling alignment with previous experimental results and accommodating future needs through simple upgrades. The small footprint of the instrument requires minimal bench space, even allowing use in a cell culture hood.

Acoustic focusing improves efficiencies compared to traditional flow cytometers
Traditional flow cytometers rely on a large volume of fluid to focus cells prior to analysis, resulting in extensive sample preparation prior to analysis and introducing the possibility of error due to artifacts created during the sample preparation process. With the Attune NxT Acoustic Focusing Cytometer, blood samples can be run without being manipulated using the No-Wash No-Lyse blood cell assay. The instrument also offers flexibility in the sample concentration that can be analyzed.

Acoustic focusing enables:
• Elimination of concentration steps
• Effective background fluorescence reduction by a simple dilution step, eliminating the need to wash fluorescence samples before analysis
• No-lyse/no-wash assays
• Analysis of samples that have low cell numbers, including CSF, stem cells, mouse bone marrow, mouse blood, and thin needle aspirates
• Acquisition of a high volume of samples in minimal time

Learn more about the Attune NxT cytometer and see all available configurations >
Learn more about how acoustic focusing works >

Attune™ Auto Sampler Waste Bottle (Applied Biosystems™)

This is a replacement bottle for waste for the Attune® Auto Sampler.