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EVOS™ 20X Objective, achromat, LWD, phase-contrast

This achromat objective is ideal for general applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 20X
• Numerical Aperture: 0.40
• Working Distance: 6.8 mm

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS Objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

EVOS™ 40X Objective, achromat, LWD, phase-contrast

This achromat objective is ideal for general applications This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 40X
• Numerical Aperture: 0.65
• Working Distance: 3.1 mm

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS Objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

EVOS™ Power Cord, Type B (North America)

This power cord is for use with EVOS XL, FL , FL Color, and FL Auto systems in countries with Type A power plugs. The Type B (North America) electrical plug is used in North America, Japan, and parts of South America, Asia, and the Middle East.

Note that Type B power cords are included with the EVOS FL Auto Imaging System.

See the complete list of power cord types and countries for more information.
Explore the entire EVOS line of imaging systems and accessories.

Slideport™ Slide Inserts (Thermo Scientific™)

The Thermo Scientific™ Slideport™ Slide Insert holds microscope slides in a frame that fits Thermo Scientific™ High Content Instruments. Slides can be imaged using many objective lenses due to two available skirt heights. The Slideport Slide Insert has been tested to image a wide range of sample types including fixed and live cells, tissue sections, tissue microarrays and beads. Contents: five Slideport Slide Inserts.

Features of Slideport Slide Inserts:

• Two skirt heights available to accommodate a variety of readers and objectives
• Any microscope or plate reader that accepts standard microplates will also accept the Slideport Slide Insert
• Image a wide range of sample types including fixed and live cells, tissue sections, tissue microarrays, and beads
• Easily stack slides for automated imaging with or without lids
• Slideport Slide Insert can be utilized at a variety of temperatures from 4°to 37°C, allowing for flexibility of storage and imaging conditions

Compatible with the Thermo Scientific High Content Instruments

EVOS™ Light Cube, Qdot™ 625

All EVOS fluorescence imaging systems contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The Qdot 625 light cube is ideal for use with Qdot 625 and other fluorophores with excitation and emission maxima near 445 and 625 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Power Cord, Type G (United Kingdom)

This power cord is for use with EVOS XL, FL , FL Color, and FL Auto systems in countries with Type G power plugs. The Type G electrical plug is used in the UK, Ireland, Hong Kong, Singapore, and other parts of Africa and Asia.

See the complete list of power cord types and countries for more information.
Explore the entire EVOS line of imaging systems and accessories.

ArrayScan™ Live Cell Module (Thermo Scientific™)

The Thermo Scientific™ ArrayScan™ Live Cell Module provides full control of temperature (ambient to 45°C), CO2 (0-10% ) and added humidity (>90% RH) to the sample chamber to maintain cell health for experiments with living cells or tissues. Therefore, cell physiology and motion can be monitored over seconds, minutes, hours or days .

Features of the ArrayScan Live Cell Module :

• Full Environmental Control—Complete environmental control (CO2, humidity, and temperature) with full reporting of CO2 and temperature.
• Kinetic Capabilities—Cell tracking with powerful kinetic analysis software provides numerous motility and kinetic calculations at the cell and well levels.
• Zeiss™ Definite Focus is a hardware-based autofocus system that maintains focus while supporting long-term imaging of live cells.

The ArrayScan Live Cell Module provides an environment similar to that of a tissue culture incubator. A proprietary cell tracking algorithm, built into the software, allows for kinetic measurements at the cell and well level.

EVOS™ Light Cube, Qdot™ 655

All EVOS fluorescence imaging systems contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The Qdot 655 light cube is ideal for use with Qdot 655 and other fluorophores with excitation and emission maxima near 445 and 625 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Power Cord, Type C (Europe)

This power cord is for use with EVOS XL, FL , FL Color, and FL Auto systems in countries with Type C power plugs. The Type C electrical plug (Europlug) is used throughout continental Europe, parts of the Middle East, and much of Africa, South America, Russia, and the former Soviet republics.

See the complete list of power cord types and countries for more information.
Explore the entire EVOS line of imaging systems and accessories.

ArrayScan™ Brightfield Module (Thermo Scientific™)

The Thermo Scientific™ ArrayScan™ Brightfield Module is an affordable option that adds white light illumination to high content screening (HCS) experiments. It allows imaging of unlabeled samples to quantify morphological features and the resulting data can be interleaved with fluorescence data from the same sample. The ArrayScan Brightfield Module has integrated software control to capture, focus, and analyze transmitted light images at single or multiple timepoints.

Features of the ArrayScan Brightfield Module :

• Capture, analyze, and visualize transmitted light images interleaved with fluorescence images
• Label-free detection
• Autofocus in transmitted light mode permitting imaging on multiple plate types, cell lines and magnifications
• Color composites can be used to verify location of fluorescent targets within a cell or organism

The ArrayScan Brightfield Module is fully integrated with the ArrayScan HCA Reader software (Thermo Scientific™ HCS Studio™ Cell Analysis Software) enabling the capture, analysis, and visualization of transmitted light images interleaved with fluorescence images at single or multiple time points.

EVOS™ Light Cube, Qdot™ 705

All EVOS fluorescence imaging systems contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The Qdot 705 light cube is ideal for use with Qdot 705 and other fluorophores with excitation and emission maxima near 445 and 705 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Power Cord, Type I (Australia)

This power cord is for use with EVOS XL, FL , FL Color, and FL Auto systems in countries with Type I power plugs. The Type I electrical plug is mainly used in Australia and New Zealand, but is also used in China and parts of Asia, South America, and Africa.

See the complete list of power cord types and countries for more information.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ 4X Objective, achromat, LWD, phase-contrast, 0.13NA/10.58WD (Invitrogen™)

This achromat objective is ideal for general applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 4X
• Numerical Aperture: 0.13
• Working Distance: 10.58 mm

Image quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

EVOS™ 10X Objective, fluorite, LWD, phase-contrast, 0.30NA/7.13WD (Invitrogen™)

This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 10X
• Numerical Aperture: 0.30
• Working Distance: 7.13 mm

Image quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

ArrayScan™ Liquid Handling Module (Thermo Scientific™)

The Thermo Scientific™ ArrayScan™ Liquid Handling Module is designed to work with the Thermo Scientific™ ArrayScan™ Live Cell Module, expanding the flexibility of the platform for analysis of complex cellular states of cells. The ArrayScan Liquid Handling Module has functionalities to aspirate, dispense, and mix, from four deck locations. The module is capable of single-tip aspirate and dispense at any location, creating a highly flexible instrument. Compatible with 6-well, 8-well, 24-well, 96-well, and 384-well plates, the ArrayScan Liquid Handling Module utilizes disposable tips with a dispense volume range of 3 to 200μL.

Features of the ArrayScan Liquid Handling Module:

• Compatible with 6-well, 8-well, 24-well, 96-well, and 384-well plates, the module utilizes disposable tips with a dispense volume range of 3 to 200μL
• Full function liquid handling from any plate or trough (3 plate and 1 quarter trough)
• Industry standard arm with single pipette tip driven by syringe pump (e.g., aspirate, dispense)
• Compatible with all ArrayScan Modules
• Dynamic access to compound and assay plate
• One-to-many and many-to-one transfer scenarios are supported

Includes:

ArrayScan Liquid Handling Module : Automated, single-tip, liquid handling robot module for the Thermo Scientific™ ArrayScan™ High Content Analysis (HCA) Readers using disposable tips (standard and liquid level sensing) to both aspirate and dispense from multiple deck and live cell chamber locations.