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ArcturusXT™ Live Cell Growth Chamber, sterile (Applied Biosystems™)

A suite of accessories has been designed to enable research on living cells using your ARCTURUSXT™ LCM Instrument. Researchers may use slides or Petri dishes to perform imaging and microdissection experiments. Following a validated protocol from ARCTURUS® LCM Systems from Applied Biosystems®; the leaders in tissue-based microdissection – the live cells may be isolated, remaining viable for subsequent re-culture or direct processing for use in downstream applications.

Live Cell Microdissection from Petri Dishes
• Isolate living cells from a Petri Dish for subsequent re-culture
• Process collected cells for downstream molecular analysis

For Research Use Only. Not for use in diagnostics procedures.

EVOS™ 4X Objective, achromat, LWD, phase-contrast

This achromat objective is ideal for general applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS® objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS® objective:

• Magnification: 4X
• Numerical Aperture: 0.13
• Working Distance: 16.9 mm

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS® objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS® objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS® Objectives selection guide
Explore the entire EVOS® line of imaging systems and accessories

EVOS™ FL Auto Calibration Slide (Invitrogen™)

The calibration slide for the EVOS™ FL Auto Imaging System is used to calibrate three parameters of the system: field of view, parfocality, and parcentration. Calibrating the parfocality ensures that the sample stays in focus when the objective is changed, and calibrating the parcentration ensures that an object in the center of the field of view will stay in the center of the field no matter which objective is being used.

The calibration slide for the EVOS FL Auto Imaging System offers you these advantages:
• Easy, accurate, and precise calibration of your microscope
• Five X-Y squares divided by grids of: 1.00, 0.200, 0.100, 0.050, and 0.025 mm
• Seven different calibration circles: 4.000, 2.000, 1.000, 0.400, 0.200, 0.100, and 0.050 mm
• Precision-ground, optical-quality glass
• Protective storage case

The calibration procedure involves two distinct steps. First, the calibration crosshair on the screen is matched to the crosshairs on the calibration slide. Then, the diameters of the reference circles on the calibration slide are measured. Each step is performed using both the monochrome and the color camera. The total time required to calibrate an objective is about 5 minutes. More information can be found in the EVOS FL Auto manual. Note that the factory-installed objectives supplied with the EVOS FL Auto Imaging System have been pre-calibrated. There is no need to calibrate them again unless they are removed and need to be re-installed.

Learn more about the EVOS FL Auto Imaging System >.
Explore the entire EVOS line of imaging systems and accessories >.

Mini PAP Pen

The Mini PAP pen draws a water repellent circle around slide mounted tissue, preventing the waste of valuable reagents by keeping the liquid pooled in a single droplet. The Mini PAP Pen is especially useful when working with multiple sections on a single slide because its tip is smaller than the PAP pen. Each pen can be used to draw about 400 circles.

CellInsight™ CX5 High Content Screening (HCS) Platform (Thermo Scientific™)

The Thermo Scientific™ CellInsight™ CX5 High Content Screening (HCS) Platform is a fast, simple-to operate, automated cellular imaging and analysis benchtop platform designed for quantitative microscopy. The CellInsight CX5 HCS Platform offers a cost effective, but powerful way to quickly automate quantitative cellular and subcellular imaging.

Features of the CellInsight CX5 HCS Platform

• Easy to Use—quickly build and optimize image-based assays with included Thermo Scientific™ HCS Studio™ Cell Analysis Software
• Flexible—designed to work with or without fluorescent labeling: The CellInsight CX5 HCS Platform can use transmitted light, as well as fluorescence, to allow users to focus on visible and/or fluorescent targets
• Affordable—automated high content imaging for all budgets
• High quality optics—Light sources, lenses, filters, and CCD detector from the top names in imaging. The five color light engine insures accuracy and stability of source lighting.
• Virtually maintenance–free operation due to state-of-the-art design and construction.
• Performance—scan and analyze a 96-well plate in under four minutes
• Compact—fits on a bench and is less than 2 ft. on all sides

Capabilities:

• Make intracellular to multicellular morphology measurements while measuring intensity of specific reporter molecules.
• Collect only the images and data you select and make multiple measurements per object, over time, if desired
• Measure subpopulations and reject undesired objects automatically
• Comprehensive pre-validated and user configurable image analysis without user scripting or programming
• Exploratory assay development tools and specific biology optimized tools built into the HCS Studio™ Cell Analysis Software
• Define biological thresholds for outputs based on control populations
• Ability to create multi-parameter gates from individual output measurements

Hardware and Software:

• Stable, reliable light source reduces variability, increases data quality, and decreases operating costs
• Fluorescence options which include near-IR wavelength and compatibility with many common fluorochromes and fluorescent proteins
• • Proprietary Thermo Scientific™ AccuFocus™ System is fast, yet flexible, for imaging cells, organelles, and low contrast targets
• Unique intelligent acQuisition (iQ) real time analysis feedback to optimize image collection and data quality
• HCS Studio Cell Analysis Software is an integrated instrument control, visualization and analysis software package for novice to advanced users
• Features Thermo Scientific™ Store Express Image and Database Software using a 10GB SQL™ database
• Software output features image/data portability so results can be imported into third-party software for documentation
• Compatible with SBS standard microplates (6-1536 well) and slides

Compatible with Thermo Scientific™ Orbitor RS Plate Mover and other Thermo Scientific™ Laboratory Automation Solutions

O-Rings for Attofluor™ Cell Chamber (Invitrogen™)

Replacement O-rings for the Attofluor® cell chamber (A7816), a durable and practical coverslip holder for viewing live-cell specimens on upright or inverted microscopes.

EVOS™ 10X Objective, achromat, LWD, phase-contrast

This achromat objective is ideal for general applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS® objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS® objective:

• Magnification: 10X
• Numerical Aperture: 0.25
• Working Distance: 6.9 mm

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS® objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS® objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS® Objectives selection guide
Explore the entire EVOS® line of imaging systems and accessories

ProPlate™ Multi-Array Slide Module (Invitrogen™)

The ProPlate multi-array system (P37004) from Grace Bio-Labs allows integration of microscope array technology with automated microtitre plate processing. Individual modules, covered with seal strips (P37002), fit into a tray (P37003), producing a modular plate with a standard microtitre plate footprint, and well spacing. The modular design allows loading of 1--4 slides per tray for plate washing and reading. Individual ProPlate modules may also be processed by hand without the use of the tray. The large well volumes and isolation of 16 individual arrays (2x8) on a single microscope slide is particularly well suited for proteomics applications, including protein expression analysis, protein-protein interactions, antibody profiling, and HTP automated analysis of multiple proteins. The ProPlate system can also be used to process cDNA or oligonucleotide arrays.

PEN Membrane Frame Slides (Applied Biosystems™)

ARCTURUS® laser capture microdissection (LCM) instruments offer a unique combination of infrared (IR) laser capture and ultraviolet (UV) laser cutting, permitting the use of any slide type and any sample preparation for microdissection. While plain glass slides are effectively used for standard IR-based LCM, polyethylene naphthalate (PEN) membrane slides facilitate this Arcturus®-exclusive dual laser function and allow you to determine the appropriate sample preparation based on your application need.

Plain Glass Slides
ARCTURUS® instruments are the only LCM systems that allow efficient use of low cost plain glass slides, and provide easy access to archival materials. We do not sell plain glass slides, though most commercially available glass slides are compatible with microdissection. For recommendations to suit your tissue type and application needs, please contact Technical Support for more information.

PEN Membrane Slides
PEN membrane slides enable the combined use of IR LCM and UV Laser Cutting. PEN Membrane Glass Slides allows the use of standard IR LCM on its own, which is the gentlest approach for isolating individual cells or small areas. PEN Membrane Frame Slides provide additional flexibility by allowing the choice of microdissection from non-dehydrated sample preparations.

For Research Use Only. Not for use in diagnostics procedures.

EVOS™ Vessel Holder, one Nunc™ or Corning™ T-75 flask (Invitrogen™)

EVOS™ vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder securely holds one Nunc™ or Corning™ T-75 flask. It was designed for the automated stage of the EVOS™ FL Auto Imaging System but can also be used with the EVOS™ XL or EVOS™ FL imaging systems with stage plate adapter (Cat. No. AMEP4691). It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS® Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS® line of imaging systems and accessories.

CoverWell™ Perfusion Chamber Gasket, 8 chambers, 9 mm diameter, 0.5 mm deep (Invitrogen™)

CoverWell perfusion chamber gaskets are designed to facilitate live-cell imaging and manipulation. These gaskets form watertight "press-to-seal" chambers with dual-access ports so reagents can be quickly added and removed. Available in single- or multi-well configurations, these gaskets allow multiple experiments to be performed on a single microscope slide or coverslip. The heat-resistant gaskets can be sterilized and used for direct culturing of cells and tissues.

EVOS™ Vessel Holder, SPL T-75 flask

EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates one SPL T-75 flask. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

Note: an adapter is needed for use of this holder on the EVOS® FL Auto automated stage.

For additional choices, see the EVOS® Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS® line of imaging systems and accessories.

EVOS™ Onstage Incubator Hose

The EVOS® Onstage Incubator Hose connects the Control Unit and the Stagetop Environmental Chamber units of the EVOS® Onstage Incubator and helps precisely control the temperature. Even small temperature shifts can have profound effects on dynamic cellular events. When performing live cell imaging studies, it is essential that the physiological and biological processes under investigation are not altered in any way. The ability to maintain temperature control with a precision of 0.1°C helps maintain cell viability and function and helps reduce the risk of biological temperature-related artifacts.

Explore the entire EVOS® line of imaging systems and accessories.

Super PAP Pen

The Super PAP pen is suitable for both immunohistochemical and fluorescent staining, drawing a water repellent circle around slide mounted tissue, & preventing the waste of valuable reagents by keeping the liquid pooled in a single droplet. In addition, it is now chemically formulated to withstand the rehydration steps performed in alcohol, as well as the high temperatures required for denaturation during in situ hybridization. Each pen can be used to draw about 800 circles.

EVOS™ Light Cube, AO Red

All EVOS® fluorescence imaging systems contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS® light cubes are user-changeable and automatically recognized by the system. The AO Red light cube is ideal for use with Acridine orange, CTC formazan, FuraRed™ and other fluorophores with excitation and emission maxima near 442 and 628 nm.

EVOS® light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS® systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS® systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS® Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS® systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS® line of imaging systems and accessories.