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EVOS™ Vessel Holder for Automated Stage, one Terasaki plate (Invitrogen™)

EVOS™ vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder securely holds one Terasaki plate and is specific for the automated stage of the EVOS™ FL Auto Imaging System. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

CellInsight™ CX5 High Content Screening (HCS) Platform (Thermo Scientific™)

The Thermo Scientific™ CellInsight™ CX5 High Content Screening (HCS) Platform is a fast, simple-to operate, automated cellular imaging and analysis benchtop platform designed for quantitative microscopy. The CellInsight CX5 HCS Platform offers a cost effective, but powerful way to quickly automate quantitative cellular and subcellular imaging.

Features of the CellInsight CX5 HCS Platform

• Easy to Use—quickly build and optimize image-based assays with included Thermo Scientific™ HCS Studio™ Cell Analysis Software
• Flexible—designed to work with or without fluorescent labeling: The CellInsight CX5 HCS Platform can use transmitted light, as well as fluorescence, to allow users to focus on visible and/or fluorescent targets
• Affordable—automated high content imaging for all budgets
• High quality optics—Light sources, lenses, filters, and CCD detector from the top names in imaging. The five color light engine insures accuracy and stability of source lighting.
• Virtually maintenance–free operation due to state-of-the-art design and construction.
• Performance—scan and analyze a 96-well plate in under four minutes
• Compact—fits on a bench and is less than 2 ft. on all sides

Capabilities:

• Make intracellular to multicellular morphology measurements while measuring intensity of specific reporter molecules.
• Collect only the images and data you select and make multiple measurements per object, over time, if desired
• Measure subpopulations and reject undesired objects automatically
• Comprehensive pre-validated and user configurable image analysis without user scripting or programming
• Exploratory assay development tools and specific biology optimized tools built into the HCS Studio™ Cell Analysis Software
• Define biological thresholds for outputs based on control populations
• Ability to create multi-parameter gates from individual output measurements

Hardware and Software:

• Stable, reliable light source reduces variability, increases data quality, and decreases operating costs
• Fluorescence options which include near-IR wavelength and compatibility with many common fluorochromes and fluorescent proteins
• • Proprietary Thermo Scientific™ AccuFocus™ System is fast, yet flexible, for imaging cells, organelles, and low contrast targets
• Unique intelligent acQuisition (iQ) real time analysis feedback to optimize image collection and data quality
• HCS Studio Cell Analysis Software is an integrated instrument control, visualization and analysis software package for novice to advanced users
• Features Thermo Scientific™ Store Express Image and Database Software using a 10GB SQL™ database
• Software output features image/data portability so results can be imported into third-party software for documentation
• Compatible with SBS standard microplates (6-1536 well) and slides

Compatible with Thermo Scientific™ Orbitor RS Plate Mover and other Thermo Scientific™ Laboratory Automation Solutions

EVOS™ Light Cube, RFP

All EVOS® fluorescence imaging systems and the Countess® II FL Automated Cell Counter contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS® light cubes are user-changeable and automatically recognized by the system. The RFP light cube is ideal for use with Alexa Fluor® 555, CellMask™ Orange, pHrodo®, RFP, SYTOX® Orange and other fluorophores with excitation and emission maxima near 531 and 593 nm.

EVOS® light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS® systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS® systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS® Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS® systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS® line of imaging systems and accessories.

EVOS™ Power Cord, Type D (India/South Africa)

This power cord is for use with EVOS® XL, FL , FL Color, and FL Auto systems in countries with Type D power plugs. The Type D electrical plug is used in parts of Africa, Asia, and South America.

See the complete list of power cord types and countries for more information.
Explore the entire EVOS® line of imaging systems and accessories.

ArcturusXT™ Live Cell Growth Chamber, sterile (Applied Biosystems™)

A suite of accessories has been designed to enable research on living cells using your ARCTURUSXT™ LCM Instrument. Researchers may use slides or Petri dishes to perform imaging and microdissection experiments. Following a validated protocol from ARCTURUS® LCM Systems from Applied Biosystems®; the leaders in tissue-based microdissection – the live cells may be isolated, remaining viable for subsequent re-culture or direct processing for use in downstream applications.

Live Cell Microdissection from Petri Dishes
• Isolate living cells from a Petri Dish for subsequent re-culture
• Process collected cells for downstream molecular analysis

For Research Use Only. Not for use in diagnostics procedures.

EVOS™ Vessel Holder for Automated Stage, two 25 x 75 mm slides

EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder securely holds two 25 x 75 mm standard or chamber microscope slides and is specific for the automated stage of the EVOS® FL Auto Imaging System. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS® Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS® line of imaging systems and accessories.

EVOS™ 10X Objective, fluorite, LWD, phase-contrast

This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS® objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS® objective:

• Magnification: 10X
• Numerical Aperture: 0.25
• Working Distance: 9.2 mm

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS® objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS® objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS® Objectives selection guide
Explore the entire EVOS® line of imaging systems and accessories

CellInsight™ CX7 High Content Analysis Platform (Thermo Scientific™)

The Thermo Scientific™ CellInsight™ CX7 High Content Analysis (HCA) Platform is a fast, automated cellular imaging and analysis platform designed for quantitative microscopy.

The CellInsight CX7 HCA Platform offers an integrated way to develop and automate high content assays using a combination of 5-color brightfield and 7-color fluorescence imaging in confocal or wide-field modes. See how the CellInsight CX7 platform compares to other high content analysis platforms from Thermo Fisher Scientific >

Features of the CellInsight CX7 HCA Platform
• 7-channel fluorescent imaging
• 5-channel bright-field imaging
• 7-channel confocal imaging
• Software and laser-based autofocus for consistent scan times
• Configured for fully automated plate handling and scanning

Capabilities
• Addresses hundreds of assay types with flexible software and hardware tools
• Scan and analyze microscope slides or most SBS-standard format microplates
• Use HCS Studio software for integrated instrument control and data analysis
• Achieve the fastest time to results by using intelligent acQuisition (iQ) real-time analysis feedback
• Compatible with Molecular Probes™ fluorescent detection reagents

Hardware and Software
• Stable and reliable light source enables reduced variability, increased data quality, and decreased operating costs
• Broad reagent options with seven-color excitation from violet to near IR for assay multiplexing
• Dual mode focusing using Software and Laser Autofocus methods to address both labelled and unlabeled sample types
• Multi-mode imaging allows for wide-field, bright-field, and confocal imaging in one run
• HCS Studio Cell Analysis Software is an integrated instrument control, visualization, and analysis software package for novice to advanced users
• Scalable with Store Image and Database Software and optional Orbitor RS Microplate handler

EVOS™ Light Cube, CFP

All EVOS® fluorescence imaging systems and the Countess® II FL Automated Cell Counter contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS® light cubes are user-changeable and automatically recognized by the system. The CFP light cube is ideal for use with CellLight® CFP, ECFP, Evans Blue, Lucifer Yellow and other fluorophores with excitation and emission maxima near 445 and 510 nm.

EVOS® light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS® systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS® systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS® Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS® systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS® line of imaging systems and accessories.

EVOS™ Vessel Holder, one Nunc™ or Corning™ T-75 flask (Invitrogen™)

EVOS™ vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder securely holds one Nunc™ or Corning™ T-75 flask. It was designed for the automated stage of the EVOS™ FL Auto Imaging System but can also be used with the EVOS™ XL or EVOS™ FL imaging systems with stage plate adapter (Cat. No. AMEP4691). It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS® Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS® line of imaging systems and accessories.

EVOS™ XL Core Attachable Mechanical Stage

The EVOS® XL Core Mechanical Stage is an alternative to the universal, fixed stage provided with the EVOS® XL Core Imaging System. This mechanical stage has X-Y axis controls and a vessel holder framework that accommodates our broad range of vessel holders for smaller sample vessel types, such as microscope slides and 35 mm dishes. Larger sample vessels, such as T-75 flasks or multi-well plates, fit directly onto the stage. Both vessel holders and large vessels are held securely in place by a stage clip during operation. The spring-loaded clip allows fast and easy vessel changes, such as during operations inside a hood or during processing of multiple samples.

Explore the entire EVOS® line of imaging systems and accessories.

PEN Membrane Frame Slides (Applied Biosystems™)

ARCTURUS® laser capture microdissection (LCM) instruments offer a unique combination of infrared (IR) laser capture and ultraviolet (UV) laser cutting, permitting the use of any slide type and any sample preparation for microdissection. While plain glass slides are effectively used for standard IR-based LCM, polyethylene naphthalate (PEN) membrane slides facilitate this Arcturus®-exclusive dual laser function and allow you to determine the appropriate sample preparation based on your application need.

Plain Glass Slides
ARCTURUS® instruments are the only LCM systems that allow efficient use of low cost plain glass slides, and provide easy access to archival materials. We do not sell plain glass slides, though most commercially available glass slides are compatible with microdissection. For recommendations to suit your tissue type and application needs, please contact Technical Support for more information.

PEN Membrane Slides
PEN membrane slides enable the combined use of IR LCM and UV Laser Cutting. PEN Membrane Glass Slides allows the use of standard IR LCM on its own, which is the gentlest approach for isolating individual cells or small areas. PEN Membrane Frame Slides provide additional flexibility by allowing the choice of microdissection from non-dehydrated sample preparations.

For Research Use Only. Not for use in diagnostics procedures.

EVOS™ Vessel Holder/Adapter for Automated Stage, with retention, multi-well plates

EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder serves two functions: it holds multi-well plates (w = 128.2 mm, h = 86.2 mm) and also acts as an adapter for the standard vessel holders (cat numbers AMEPVH001 thru AMEPVH018). This vessel holder/adapter is specific for the automated stage of the EVOS® FL Auto Imaging System and has a lever for added convenience. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS® Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS® line of imaging systems and accessories.

EVOS™ 20X Objective, fluorite, LWD, phase-contrast

This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS® objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS® objective:

• Magnification: 20X
• Numerical Aperture: 0.40
• Working Distance: 3.1 mm

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS® objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS® objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS® Objectives selection guide
Explore the entire EVOS® line of imaging systems and accessories

EVOS™ Light Cube, TagBFP

All EVOS® fluorescence imaging systems and the Countess® II FL Automated Cell Counter contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS® light cubes are user-changeable and automatically recognized by the system. The TagBFP light cube is ideal for use with CascadeBlue™, Pacific Blue™, TagBFP and other fluorophores with excitation and emission maxima near 390 and 447 nm.

EVOS® light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS® systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS® systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS® Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS® systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS® line of imaging systems and accessories.