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EVOS™ XL Core Attachable Mechanical Stage

The EVOS XL Core Mechanical Stage is an alternative to the universal, fixed stage provided with the EVOS XL Core Imaging System. This mechanical stage has X-Y axis controls and a vessel holder framework that accommodates our broad range of vessel holders for smaller sample vessel types, such as microscope slides and 35 mm dishes. Larger sample vessels, such as T-75 flasks or multi-well plates, fit directly onto the stage. Both vessel holders and large vessels are held securely in place by a stage clip during operation. The spring-loaded clip allows fast and easy vessel changes, such as during operations inside a hood or during processing of multiple samples.

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EVOS™ DVI Video Capture Peripheral

The EVOS DVI Video Capture Peripheral is a portable DVI-to-USB frame grabber ideally suited to capture video output from time-lapse acquisitions in real time. It is compatible with all EVOS imaging systems that offer DVI output and can capture resolutions up to 1920 x 1200 pixels at a capture rate of up to 30 frames per second.

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EVOS™ FL Auto Calibration Slide Invitrogen™

The calibration slide for the EVOS FL Auto Imaging System is used to calibrate three parameters of the system: field of view, parfocality, and parcentration. Calibrating the parfocality ensures that the sample stays in focus when the objective is changed, and calibrating the parcentration ensures that an object in the center of the field of view will stay in the center of the field no matter which objective is being used.

The calibration slide for the EVOS FL Auto Imaging System offers you these advantages:
• Easy, accurate, and precise calibration of your microscope
• Five X-Y squares divided by grids of: 1.00, 0.200, 0.100, 0.050, and 0.025 mm
• Seven different calibration circles: 4.000, 2.000, 1.000, 0.400, 0.200, 0.100, and 0.050 mm
• Precision-ground, optical-quality glass
• Protective storage case

The calibration procedure involves two distinct steps. First, the calibration crosshair on the screen is matched to the crosshairs on the calibration slide. Then, the diameters of the reference circles on the calibration slide are measured. Each step is performed using both the monochrome and the color camera. The total time required to calibrate an objective is about 5 minutes. More information can be found in the EVOS FL Auto manual. Note that the factory-installed objectives supplied with the EVOS FL Auto Imaging System have been pre-calibrated. There is no need to calibrate them again unless they are removed and need to be re-installed.

Learn more about the EVOS FL Auto Imaging System >.
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Dell Monitor Power Cord, Type I (China, CCC) Invitrogen™

This Dell Monitor Power Cord is for use with the Dell monitor that is part of the EVOS FL Auto 2 Imaging System in countries using Type I (China) power plugs. This product meets CCC requirements (certificate for China compulsory product certification).

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Note that one Type B (North America) power cord for the Dell monitor is included with the EVOS FL Auto 2 Imaging System.

EVOS™ Condenser Slider, Diffusion

Condenser sliders are used to improve illumination under certain conditions and several are available for the EVOS imaging systems, including a diffuser slider, pinhole slider, and meniscus sliders. Due to variances in sample size, color and thickness, actual slider use may differ from suggestions. This diffusion slider is intended for brightfield applications at 2x or 4x magnification for flat-field illumination. Note that a diffusion condenser slider is included with EVOS XL Imaging System.

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EVOS™ Onstage Incubator Hose

The EVOS Onstage Incubator Hose connects the Control Unit and the Stagetop Environmental Chamber units of the EVOS Onstage Incubator and helps precisely control the temperature. Even small temperature shifts can have profound effects on dynamic cellular events. When performing live cell imaging studies, it is essential that the physiological and biological processes under investigation are not altered in any way. The ability to maintain temperature control with a precision of 0.1°C helps maintain cell viability and function and helps reduce the risk of biological temperature-related artifacts.

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EVOS™ Condenser Slider, Meniscus A (clear liquids)

Condenser sliders are used to improve illumination under certain conditions and several are available for the EVOS imaging systems, including a diffuser slider, pinhole slider, and meniscus sliders. Due to variances in sample size, color and thickness, actual slider use may differ from suggestions. This Meniscus A slider is intended for brightfield applications at 2x magnification with low-volume samples in multi-well plates and with clear liquids.

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EVOS™ Onstage Incubator Gas Line

The EVOS Onstage Incubator Gas Line connects the Control Unit of the EVOS Onstage Incubator to user-supplied gas sources. When performing live-cell imaging studies, it is essential that the environmental conditions are precisely controlled. The EVOS Onstage Incubator has three gas line ports, enabling studies of cells under normal, as well as hypoxic, conditions. The EVOS Onstage Incubator is supplied with 25 feet of 1/8 in ID, 1/4 in OD tubing. The EVOS Onstage Incubator Gas Line provides an additional 25 feet of this tubing; this can be helpful in situations where gas supplies are not located in close proximity to the EVOS Onstage Incubator.

EVOS™ 10X Objective, fluorite, LWD

This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 10X
• Numerical Aperture: 0.30
• Working Distance: 8.3 mm

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS Objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

PEN Membrane Frame Slides for Live Cell Microdissection Applied Biosystems™

A suite of accessories has been designed to enable research on living cells using your ArcturusXT™ LCM Instrument. Researchers may use slides or Petri dishes to perform imaging and microdissection experiments. Following a validated protocol from Arcturus® LCM Systems from Applied Biosystems—the leaders in tissue-based microdissection—the live cells may be isolated, remaining viable for subsequent re-culture or direct processing for use in downstream applications.

Polyethylene naphthalate (PEN) Membrane Frame Slides for Live Cell Microdissection
• Specially optimized for use with live cell experiments
• Take advantage of the CapSure® LCM Cap technology to collect cells for downstream processing

For Research Use Only. Not for use in diagnostics procedures.

EVOS™ 100X Oil Objective, achromat, coverslip-corrected

This achromat objective is ideal for general applications. This is a coverslip-corrected objective that is optimized for imaging through #1.5 coverslips. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 100X
• Numerical Aperture: 1.25
• Working Distance: 0.15 mm
• Immersion: Oil

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas. Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS Objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

Olympus™ 60X Objective, Fluorite, NA 0.90, WD 0.2 mm, correction collar (vessel thickness 0.11–0.23 mm)

This fluorite (semi-apochromat) objective is equipped with an adjustable collar that affords flexibility in imaging through vessels with a thickness from 0.11 to 0.23 mm. With four-color correction for spherical and chromatic aberrations, it delivers exceptional image quality. For the sharpest image simply set the collar to the thickness of the vessel/coverslip you are using. If the thickness is unknown, turn the collar in either direction while monitoring the sample focus. All Olympus objectives are compatible with EVOS cell imaging systems and offer outstanding optical performance from visible to near-infrared light. The extensive selection of EVOS and Olympus objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this Olympus objective:
• Magnification: 60X
• Numerical Aperture: 0.90
• Working Distance: 0.2 mm
• Superb image quality

Image quality
Microscope objectives may be the most important components of a microscope because they are responsible for primary image formation. High image quality is crucial to experimental success and often a requirement for publication; Olympus objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture experience, and the broad selection of objectives means that you can choose the optimal objective for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas. Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide.
Explore the entire EVOS line of imaging systems and accessories ›

EVOS™ Condenser Slider, Meniscus B (semi-opaque liquids)

Condenser sliders are used to improve illumination under certain conditions and several are available for the EVOS imaging systems, including a diffuser slider, pinhole slider, and meniscus sliders. Due to variances in sample size, color and thickness, actual slider use may differ from suggestions. This Meniscus B slider is intended for brightfield applications at 2x magnification with high-volume samples in multi-well plates and with semi-opaque liquids.

Explore the entire EVOS line of imaging systems and accessories.

Gene Frame, 25 µL (1.0 x 1.0 cm) Thermo Scientific™

Thermo Scientific Gene Frames have adhesives on either side of the frame which are of differing strengths; the side which adheres to the glass has an easy release adhesive whereas the reverse, which sticks to the coverslip, forms a stronger bond. This means that when the coverslip is removed, due to the disparity in the strengths of the different adhesives, the frame is removed simultaneously with no sticky residue being left behind on the slide.

Features of Thermo Scientific Gene Frames:

• Gas tight sealing prevents reagent loss due to evaporation
• Can withstand temperatures of 97°C
• Designed for use with standard microscope slides
• Frame and coverslip can be removed simultaneously leaving no residue
• Can be autoclaved or sterilized by UV irradiation
• All Gene Frame packs are supplied with 100 coverslips
• Rigid coverslips for ease of handling and uniform hybridizations
• Thickness of Gene Frames: 0.25 mm
• The 25 µL size have a 1.0 x 1.0 cm chamber

Applications

• In-situ PCR

Related Products
Gene Frame, 65 µL (1.5 x 1.6 cm)
Gene Frame, 125 µL (1.7 x 2.8 cm)

Olympus 100X Oil Objective, super-apochromat, coverslip-corrected

This super-apochromat objective offers exceptional Apo correction from the UV through IR ranges. Parfocal for both visible and IR imaging, it is designed for the most demanding research applications. This is a coverslip-corrected objective that is optimized for imaging through #1.5 coverslips. All Olympus objectives are compatible with EVOS Cell Imaging systems and offer outstanding optical performance from visible light to near infrared light. The extensive choice of EVOS and Olympus objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this Olympus objective:

• Magnification: 100X
• Numerical Aperture: 1.40
• Working Distance: 0.13 mm
• Immersion: Oil

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication. Olympus objectives are widely recognized for their quality and performance across the visible spectrum to near infrared light. The broad selection of EVOS and Olympus objectives means you have a range of options for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas. Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS & Olympus objectives selection guide
Explore the entire EVOS line of imaging systems and accessories
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