Shop All Imaging & Microscopy Instruments & Accessories

Press-to-Seal™ Silicone Isolator, one well, 20 mm diameter, 0.5 mm deep Invitrogen™

For the ultimate in utility and flexibility, press-to-seal silicone isolators can be customized to meet the needs of the researcher. They are available either pre-cut to specific interior dimensions or as sheet material that can be easily trimmed to prepare customized enclosures. These isolators are autoclavable and non-cytotoxic and can be used to isolate cells in culture dishes or to separate specimens on microscope slides.

Olympus™ 2X Objective, PlanApo (0.08 NA) Thermo Scientific™

The Olympus™ 2x (0.08NA) PlanApo objective lens for the Thermo Scientific™ CellInsight™ CX5 HCS Platform is made by the world renowned optics company, Zeiss. The Olympus 2x (0.08NA) PlanApo lens represents the finest in optical with a magnification power suited for subcellular imaging, and a numerical aperture which strikes a balance between resolution and versatility.

Press-to-Seal™ Silicone Sheet, 13 cm x 18 cm, 0.5 mm thick Invitrogen™

For the ultimate in utility and flexibility, press-to-seal silicone isolators can be customized to meet the needs of the researcher. They are available either pre-cut to specific interior dimensions or as sheet material that can be easily trimmed to prepare customized enclosures. These isolators are autoclavable and non-cytotoxic and can be used to isolate cells in culture dishes or to separate specimens on microscope slides.

Press-to-Seal™ Silicone Isolator with Adhesive, eight wells, 9 mm diameter, 0.5 mm deep Invitrogen™

Press-to-Seal silicone isolators are removable hydrophobic barriers that provide researchers with the ultimate in flexibility for isolating specimens. They may be used to isolate cells grown in culture dishes or to separate specimens on microscopy slides during staining procedures. The silicone material is autoclavable and adheres to any smooth surface.

EVOS™ Vessel Holder, four 35-mm Petri dishes

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder accommodates four 35 mm Petri dishes. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

Note: an adapter is needed for use of this holder on the EVOS FL Auto automated stage.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

Olympus™ 4X Objective, U Plan S-Apo (0.16 NA) Thermo Scientific™

The Olympus™ 4x (0.16NA) U Plan S-Apo objective lens for the Thermo Scientific™ CellInsight™ CX5 High Content Screening (HCS) Platform is made by the world renowned optics company, Zeiss. The Olympus 4x (0.16NA) U Plan S-Apo lens represents the finest in optical with a magnification power suited for subcellular imaging, and a numerical aperture which strikes a balance between resolution and versatility.

EVOS™ 50X Oil Objective, achromat, coverslip-corrected

This achromat objective is ideal for general applications. This is a coverslip-corrected objective that is optimized for imaging through #1.5 coverslips. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 50X
• Numerical Aperture: 0.95
• Working Distance: 0.19
• Immersion: Oil

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS Objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

Press-to-Seal™ Silicone Sheet, 13 cm x 18 cm, 1.0 mm thick Invitrogen™

For the ultimate in utility and flexibility, press-to-seal silicone isolators can be customized to meet the needs of the researcher. They are available either pre-cut to specific interior dimensions or as sheet material that can be easily trimmed to prepare customized enclosures. These isolators are autoclavable and non-cytotoxic and can be used to isolate cells in culture dishes or to separate specimens on microscope slides.

Press-to-Seal™ Silicone Isolator with Adhesive, eight wells, 9 mm diameter, 1.0 mm deep Invitrogen™

Press-to-Seal silicone isolators are removable hydrophobic barriers that provide researchers with the ultimate in flexibility for isolating specimens. They may be used to isolate cells grown in culture dishes or to separate specimens on microscopy slides during staining procedures. The silicone material is autoclavable and adheres to any smooth surface.

Gene Frame, 65 µL (1.5 x 1.6 cm) Thermo Scientific™

Thermo Scientific Gene Frames have adhesives on either side of the frame which are of differing strengths; the side which adheres to the glass has an easy release adhesive whereas the reverse, which sticks to the coverslip, forms a stronger bond. This means that when the coverslip is removed, due to the disparity in the strengths of the different adhesives, the frame is removed simultaneously with no sticky residue being left behind on the slide.

Features of Thermo Scientific Gene Frames:

• Gas tight sealing prevents reagent loss due to evaporation
• Can withstand temperatures of 97°C
• Designed for use with standard microscope slides
• Frame and coverslip can be removed simultaneously leaving no residue
• Can be autoclaved or sterilized by UV irradiation
• All Gene Frame packs are supplied with 100 coverslips
• Rigid coverslips for ease of handling and uniform hybridizations
• Thickness of Gene Frames: 0.25 mm
• The 65 µL size have a 1.5 x 1.6 cm chamber

Applications

• In-situ PCR

Related Products
Gene Frame, 25 µL (1.0 x 1.0 cm)
Gene Frame, 125 µL (1.7 x 2.8 cm)

Olympus 20X Objective, super-apochromat, coverslip-corrected

This super-apochromat objective offers exceptional Apo correction from the UV through IR ranges. Parfocal for both visible and IR imaging, it is designed for the most demanding research applications. This is a coverslip-corrected objective that is optimized for imaging through #1.5 coverslips. All Olympus objectives are compatible with EVOS Cell Imaging systems and offer outstanding optical performance from visible light to near infrared light. The extensive choice of EVOS and Olympus objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this Olympus objective:

• Magnification: 20X
• Numerical Aperture: 0.75
• Working Distance: 0.65 mm

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication. Olympus objectives are widely recognized for their quality and performance across the visible spectrum to near infrared light. The broad selection of EVOS and Olympus objectives means you have a range of options for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas. Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS & Olympus objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

EVOS™ Vessel Holder, two 60-mm Petri dishes

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder accommodates two 60 mm Petri dishes. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

Note: an adapter is needed for use of this holder on the EVOS FL Auto automated stage.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ 40X Objective, fluorite, LWD, phase-contrast, 0.65NA/1.79WD Invitrogen™

This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 40X
• Numerical Aperture: 0.65
• Working Distance: 1.79 mm

Image quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

Onstage Incubator for CellInsight CX5/NXT HCS Platforms Thermo Scientific™

The Invitrogen Onstage Incubator for CellInsight NXT/CX5 High Content Screening (HCS) Platforms is an environmental chamber designed specifically CellInsight NXT and CellInsight CX5 series platforms. As an optional module, it enables precise control of temperature, humidity, and three gases for time-lapse imaging of live cells under both physiological and non-physiological conditions. Environmental settings and image acquisition parameters are all seamlessly integrated into the HCS Studio software creating a powerful, walk-away platform for live cell quantitative imaging. The Onstage Incubator for CellInsight NXT/CX5 HCS Platforms can be upgraded at any time to existing compatible units.

Advantages of the Onstage Incubator for CellInsight NXT/CX5 HCS Platforms:
• Fully integrated environmental chamber for live cell time-lapse imaging
• Precisely maintain physiological or non-physiological conditions
• Simple scheduling to define frequency of imaging over a time course
• Powerful kinetic and motility measurements that increase information gathered from each cell over time
• Field upgradeable
• Compatible with fixed endpoint screening using the Thermo Scientific Orbitor RS Plate Mover

Requirements
The Onstage Incubator for CellInsight NXT/CX5 HCS Platforms requires the latest version of HCS Studio software and STore Image and Data Management Software.

O-Rings for Attofluor™ Cell Chamber Invitrogen™

Replacement O-rings for the Attofluor® cell chamber (A7816), a durable and practical coverslip holder for viewing live-cell specimens on upright or inverted microscopes.
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