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HybriSlip™ Hybridization Cover, 22 mm x 22 mm, RNase free (Invitrogen™)

HybriSlip hybridization covers are nuclease free, ready to use and designed specifically for in situ hybridization. These hydrophobic coverslips do not require lengthy preparation or blocking procedures to prevent probe trapping or binding. HybriSlip covers are heat resistant and do not curl, even at high temperatures. They are ideal for denaturation steps or in situ PCR incubations.[The PCR process is covered by patents owned by Hoffmann-LaRoche, Inc. Purchase of these products does not convey a license under these patents.]

HybriSlip™ Hybridization Cover, 40 mm x 22 mm, RNase free (Invitrogen™)

HybriSlip hybridization covers are nuclease free, ready to use and designed specifically for in situ hybridization. These hydrophobic coverslips do not require lengthy preparation or blocking procedures to prevent probe trapping or binding. HybriSlip covers are heat resistant and do not curl, even at high temperatures. They are ideal for denaturation steps or in situ PCR incubations.[The PCR process is covered by patents owned by Hoffmann-LaRoche, Inc. Purchase of these products does not convey a license under these patents.]

HybriSlip™ Hybridization Cover, 60 mm x 22 mm, RNase free (Invitrogen™)

HybriSlip hybridization covers are nuclease free, ready to use and designed specifically for in situ hybridization. These hydrophobic coverslips do not require lengthy preparation or blocking procedures to prevent probe trapping or binding. HybriSlip covers are heat resistant and do not curl, even at high temperatures. They are ideal for denaturation steps or in situ PCR incubations.[The PCR process is covered by patents owned by Hoffmann-LaRoche, Inc. Purchase of these products does not convey a license under these patents.]

EVOS™ Power Cord, Type H (Israel)

This power cord is for use with EVOS XL, FL , FL Color, and FL Auto systems in countries with Type H power plugs. The Type H plug is used in Israel and Gaza.

See the complete list of power cord types and countries for more information.
Explore the entire EVOS line of imaging systems and accessories.

CultureWell™ Chambered Coverglass for Cell Culture, 16 wells per coverglass (Invitrogen™)

The CultureWell™ removable chambered coverglass for cell culture is ideal for cell culture and fluorescence imaging applications. The chambered coverglass—provided sterile and ready to use—contains 16 wells that can each hold up to 250 µL, allowing cells to be cultured in a number of different conditions on a single slide. When the cells are ready to be imaged, coverglass removal is made easy by the use of a simple tool (Cat. No. C37010) that separates the parts without the need for excessive force, eliminating the risk of coverglass breakage.

HybriWell™ Hybridization Sealing System, 40 mm x 21 mm chamber, 0.15 mm deep (Invitrogen™)

The HybriWell hybridization sealing system is a coverslip—seal combination that forms chambers optimized for in situ hybridization work. Each disposable incubation chamber seals securely to a microscope slide, providing a limited-volume chamber for reagent addition. Solutions are easily added through dual-access ports without disrupting the specimen. The hydrophobic coverslips are nuclease free and will not trap or bind probes, allowing uniform distribution of the reagent over the specimen. The HybriWell sealing system also includes a quick-seal tool to secure the hydrophobic cover to the microscope slide, as well as nuclease-free adhesive seal tabs to cover the access ports (seal tabs are also available separately).

EVOS™ Light Cube, DAPI

All EVOS fluorescence imaging systems and the Countess II FL Automated Cell Counter contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The DAPI light cube is ideal for use with Acridine, Alexa Fluor 350, DAPI, Hoechst, NucBlue, MarinaBlue, Pacific Blue and other fluorophores with excitation and emission maxima near 357 and 447 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.

Coverglass Removal Tool (Invitrogen™)

The Coverglass Removal Tool is intended for use with a CultureWell™ Chambered Coverglass for Cell Culture (Cat. Nos. C37000, C37005). This tool enables you to easily separate the chambered upper structure from the coverglass without the need for excessive force, minimizing the risk of coverglass breakage.

EVOS™ Light Cube, GFP

All EVOS fluorescence imaging systems and the Countess II FL Automated Cell Counter contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The GFP light cube is ideal for use with Alexa Fluor 488, Calcein, AM, CellLight GFP, CellTracker Green, CellRox Green, FITC, Fluo-4, GFP, and other fluorophores with excitation and emission maxima near 470 and 510 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Vessel Holder, Nunc™ T-75 flask

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder accommodates one Nunc T-75 flask. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

Note: an adapter is needed for use of this holder on the EVOS FL Auto automated stage.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

Olympus 100X Oil Objective, super-apochromat, coverslip-corrected

This super-apochromat objective offers exceptional Apo correction from the UV through IR ranges. Parfocal for both visible and IR imaging, it is designed for the most demanding research applications. This is a coverslip-corrected objective that is optimized for imaging through #1.5 coverslips. All Olympus objectives are compatible with EVOS Cell Imaging systems and offer outstanding optical performance from visible light to near infrared light. The extensive choice of EVOS and Olympus objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this Olympus objective:

• Magnification: 100X
• Numerical Aperture: 1.40
• Working Distance: 0.13 mm
• Immersion: Oil

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication. Olympus objectives are widely recognized for their quality and performance across the visible spectrum to near infrared light. The broad selection of EVOS and Olympus objectives means you have a range of options for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas. Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS & Olympus objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

EVOS™ Light Cube, CFP-YFP EM

All EVOS fluorescence imaging systems and the Countess II FL Automated Cell Counter contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The CFP-YFP EM light cube is ideal for use with CFP-YFP FRET emission and other fluorophores with excitation and emission maxima near 445 and 542 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Light Cube, RFP

All EVOS fluorescence imaging systems and the Countess II FL Automated Cell Counter contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The RFP light cube is ideal for use with Alexa Fluor 555, CellMask Orange, pHrodo, RFP, SYTOX Orange and other fluorophores with excitation and emission maxima near 531 and 593 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.

Slideport™ Slide Inserts (Thermo Scientific™)

The Thermo Scientific™ Slideport™ Slide Insert holds microscope slides in a frame that fits Thermo Scientific™ High Content Instruments. Slides can be imaged using many objective lenses due to two available skirt heights. The Slideport Slide Insert has been tested to image a wide range of sample types including fixed and live cells, tissue sections, tissue microarrays and beads. Contents: three Slideport Slide Inserts.

Features of Slideport Slide Inserts:

• Two skirt heights available to accommodate a variety of readers and objectives
• Any microscope or plate reader that accepts standard microplates will also accept the Slideport Slide Insert
• Image a wide range of sample types including fixed and live cells, tissue sections, tissue microarrays, and beads
• Easily stack slides for automated imaging with or without lids
• Slideport Slide Insert can be utilized at a variety of temperatures from 4°to 37°C, allowing for flexibility of storage and imaging conditions

Compatible with the Thermo Scientific High Content Instruments

EVOS™ Light Cube, Qdot™ 800

All EVOS fluorescence imaging systems contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The Qdot 800 light cube is ideal for use with Qdot 800 and other fluorophores with excitation and emission maxima near 445 and 800 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.