Shop All Real-Time PCR Instruments & Accessories

Twister™ Automation Robot for QuantStudio™ Systems (Applied Biosystems™)

The Twister® Automation Robot for QuantStudio™ Systems is designed for use with the QuantStudio™ 12K Flex and QuantStudio™ 7 Flex real-time PCR systems, enabling real-time PCR in automated environments for increased sample throughput. This robot provides extended hours of walk-away automation so you can make better use of your research time.

Highlights:

• Runs up to 102 individual 384-well reaction plates, 102 TaqMan® Array microfluidic cards, 48 96-well plates, or 62 Fast 96-well plates
• Bench-top system with a compact footprint
• Removable stacks offer effortless expansion or contraction so the system does not takes up more valuable lab space than necessary

QuantStudio™ 12K Flex Real-Time PCR System, Array Card block, desktop (Applied Biosystems™)

The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The QuantStudio™ 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray® plates, TaqMan® array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it’s now or in the future, for maximum flexibility and consistency in your research.

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

The QuantStudio™ 12K Flex System’s intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray® components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

Flexibility & Scalability
Includes five interchangeable blocks: The QuantStudio™ 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan® array cards, and OpenArray® plates, for effortless scaling from 1 to 12,000 data points in a single run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan® array cards and OpenArray® plates.

• Compatible with a full range of TaqMan® assays:
The QuantStudio™ 12K Flex System is optimized to enable clear, clean data for all TaqMan® assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.

• Provides a seamless switch from qPCR to digital PCR:
Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio™ Digital PCR kits and DigitalSuite™ Software with the QuantStudio™ OpenArray® block.

• Delivers peace of mind:
Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.

Maximum Throughput
Enhanced OptiFlex® System: Building upon Applied Biosystems’ proprietary technology, the Enhanced OptiFlex® System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single run.

• Unprecedented coverage and sample throughput:
The OpenArray® format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.

• Simple OpenArray® workflow:
QuantStudio™ Open Array® plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio™ 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.

• Integrated analysis and quality systems:
New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite™ v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.

QuantStudio™ 12K Flex Software
• Intuitive interface and innovative design for novice and experienced users
• Convenient walk-away operation
• Improved data analysis across block types
• Fully compatible with high-throughput environments

Expandable Software Architecture
Optional add-ons are available to upgrade the existing software:
21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.

• High Resolution Melt (HRM):
Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.

• DigitalSuite™ Software v1.0:
For OpenArray® block users needing sensitivity for rare targets and precision to measure small differences in target abundance.

• ExpressionSuite Software v1.0:
our latest stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray® plates.

For Research Use Only. Not for use in diagnostics procedures.

QuantStudio™ 5 Real-Time PCR System for Human Identification, 96-well, 0.2 mL, desktop (Applied Biosystems™)

The Applied Biosystems QuantStudio 5 Real-Time PCR System for Human Identification with tower computer is the latest addition to our human identification (HID) real-time PCR family of products. This instrument is designed for both new and experienced users who need a simple, reliable, and affordable real-time PCR system that doesn’t require compromise on performance or quality. When used with our latest advances in quantification chemistry and HID Real-Time PCR Analysis Software, this system offers a sensitive, robust solution for forensic DNA quantification. It also helps prepare laboratories for future quantification technologies. With maximum dye versatility, this HID solution offers accurate, trusted results in a small benchtop footprint.

Features of the QuantStudio 5 Real-Time PCR System for Human Identification:

Streamlined
• Smaller foot print: 27 W x 50 D x 40 H cm, designed to maximize horizontal and vertical bench space
• Front-facing fully motorized block

Interactive
• Touch screen interface enables viewing of amplification plots
• Easy and intuitive interface

Reliable
• Bright light LED median lifetime greater than 5 years
• Less frequent maintenance
• 10 GB on-board memory for storage of 2,000–5,000 runs
• HID-validated system
• 0.2 mL standard 96-well format

Innovative
• Multiplexing capability for up to six dyes, enabling future quantification technology
• Precise temperature control with Peltier-controlled blocks (known as VeriFlex zones)
• USB interface

Real-Time PCR applications
Real-time PCR is a flexible technique that can be used for multiple forensic applications:
• Forensic database
    --Laboratories without direct amplification workflows
• Forensic casework
    --Confirmatory screening of male DNA
    --Sexual assault sample workflow
    --Quality and quantity sample assessment

StepOne™ Real-Time PCR System, desktop (Applied Biosystems™)

The StepOne™ Real-Time PCR System is a 48-well, low-throughput Real-Time PCR instrument perfect for both first-time and experienced users. The StepOne™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 3-color optical recording, the StepOne™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications. Includes a tower computer.

Features of the The StepOne™ Real-Time PCR System include:

• Advanced software and instrumentation for performing a wide array of genomic assays.
• Sensitive 3-color optical LED recording system
• Intuitive and robust software perfect for both first-time and advanced users
• Simple and flexible instrument set-up and usage

StepOne™ Software
The StepOne™ Software included with the StepOne™ Real-Time PCR System runs on Windows® XP, Window Vista®, and Windows® 7 operating systems and provides instrument control, data collection, and data analysis capabilities. This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.

Rapid and Simple Assay Set-Up
This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOne™ Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.

Sensitive LED-Based 3-Color Fluorescence Reading
The StepOne™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, and ROX™ dyes. This cost-effective, 3-color, 48-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.

Compatible with Many Genomic Analysis Techniques
Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOne™ Real-Time PCR System and software. The StepOne™ System supports many Real-Time PCR applications, including the following:

• SNP Genotyping
• Gene Expression Analysis
• MicroRNA Expression
• Translocation Analysis
• Gene Detection
• Viral Load Analysis

The included StepOne™ Software supports a variety of analysis methods, including the following:

• Standard Curve (absolute quantitation)
• Relative Standard Curve
• Comparative Ct (ΔΔCt) (relative quantitation)
• Genotyping and Presence/Absence
• Melt Curve Analysis
High Resolution Melting (additional software required)

Versatile Instrument Configuration Options
The ultra-compact footprint of a StepOne™ System can be installed in multiple distinct configurations, providing unmatched flexibility and convenience that can allow a fit in any laboratory (Fig 2). The StepOne™ Real-Time PCR System can be used via a PC, with a PC connected to a Local Area Network (LAN), or as a stand-alone instrument (PC-free). Each StepOne™ Real-Time PCR System is factory-calibrated for optical and thermal accuracy, so simply remarkable Real-Time PCR results can be obtained right out of the box.

Real-Time Data Monitoring, Dissemination, and Storage
The system measures amplification as it occurs, allowing you to monitor the progress of your experiment cycle by cycle, either on the machine or remotely. Your data is stored on the instrument itself, and can be viewed and stored automatically via remote access or transferred via email or a USB flash drive. Data can also be conveniently exported as PowerPoint®, Excel®, and graphical image files.

Expanded Gene Expression Capabilities
The advanced software provided with the StepOne™ Real-Time PCR System now includes the powerful Gene Expression Study Package. This software package allows for greater flexibility and accuracy in your gene expression assays through:

• Analysis of an unlimited number of plates in one study
• Sorting of data by biological or technical replicate group
• Use of multiple endogenous controls
• Assay efficiency correction

High Resolution Melting Capability
Now with High Resolution Melt Software v3.0 you can perform post-PCR sequence variation analysis with the StepOne™ Real-Time PCR System. This separately purchased software simplifies set-up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel. HRM Software v3.0 also has an improved clustering algorithm for increased sensitivity and accuracy, and the ability to conduct separate analyses of multiple assays run on a single plate.

For Research Use Only. Not for use in diagnostics procedures.

QuantStudio™ 12K Flex OpenArray™ Block Upgrade Kit (with Accufill™ System) (Applied Biosystems™)

The QuantStudio™ 12K Flex OpenArray™ Block Upgrade Kit (with Accufill™ System) can accelerate genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.

Plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™. Easily load 4 arrays into the QuantStudio 12K Flex instrument to achieve over 12,000 data points.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

QuantStudio™ 12K Flex Real-Time PCR System, OpenArray™ block, Accufill™ System (Applied Biosystems™)

The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The QuantStudio™ 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray® plates, TaqMan® array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it’s now or in the future, for maximum flexibility and consistency in your research. Note: this particular system includes an OpenArray block and AccuFill™ sample handling system. For a package that does not include an AccuFill system, please see Cat. No. 4472380.

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

The QuantStudio™ 12K Flex System’s intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray® components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

Flexibility & Scalability
Includes five interchangeable blocks: The QuantStudio™ 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan® array cards, and OpenArray® plates, for effortless scaling from 1 to 12,000 data points in a single 100 minute run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan® array cards and OpenArray® plates.
Compatible with a full range of TaqMan® assays: The QuantStudio™ 12K Flex System is optimized to enable clear, clean data for all TaqMan® assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.
Provides a seamless switch from qPCR to digital PCR: Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio® Digital PCR kits and DigitalSuite Software with the QuantStudio™ OpenArray ® block.
Delivers peace of mind: Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.

Horsepower
Enhanced OptiFlex™ System: Building upon Applied Biosystems’ proprietary technology, the Enhanced OptiFlex™ System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single 100 minute run.
Unprecedented coverage and sample throughput: The OpenArray® format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.
Simple OpenArray® workflow: QuantStudio™ Open Array® plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio™ 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.
Tools to maximize your resources: New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.

QuantStudio™ 12K Flex Software
• Intuitive interface and innovative design for novice and experienced users
• Convenient walk-away operation
• Improved data analysis across block types
• Fully compatible with high-throughput environments

Expandable Software Architecture
Optional add-ons are available to upgrade the existing software:
21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.
High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.
DigitalSuite Software v1.0: For OpenArray™ block users needing sensitivity for rare targets and precision to measure small differences in target abundance.
ExpressionSuite software v1.0: our latest stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray® plates.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

PikoReal 96, validation plate (1 plate) (Thermo Scientific™)

Thermo Scientific PikoReal 96 Validation Plate is designed to verify the performance of the Thermo Scientific PikoReal 96 RealTime PCR System.

The Validation Plate is a pre-loaded and sealed 96-well plate. Each well contains 10 µl of reaction mixture with the same quantity of Lambda DNA template in each reaction. The qPCR mixture of the PikoReal 96 Validation Plate is green to visualize the reaction mixture in each well and evaluate equal volume in each well before and after cycling.

QuantStudio™ 1 Real-Time PCR System, 96-well, 0.2 mL (Applied Biosystems™)

The Applied Biosystems QuantStudio 1 Real-Time PCR System makes quality and reliability the practical choice. This affordable instrument offers basic features ideal for those new to qPCR or those who have a limited budget. Despite its affordability, the QuantStudio 1 system offers the quality, reliability, and advanced user experience of our QuantStudio family of instruments.

Download Quantstudio 1 Design & Analysis Software >   |   Release notes

Features of the QuantStudio 1 system include:
Touchscreen—a large 8.5-inch interactive touchscreen
Fixed block—one fixed 96-well, 0.2-mL isothermal block with an easy-to-open manual drawer
Reaction volume—10–100 μL
Multiplexing capability—offers the three most commonly used excitation and emission filters: FAM, VIC, and ROX
Protocols—pre-optimized protocol templates
Software—intuitive software that supports analysis via web browser or desktop computer
Dyes—three filters calibrated for FAM/SYBR Green/SYTO 9, VIC/HEX/TET/JOE, and JUN/ROX/Texas Red dyes
Sensitivity—detect differences as small as 1.5-fold quantities in singleplex reactions
Dimensions (H x W x D) and weight—40 x 27 x 50 cm, <26 kg

Thermo Fisher Connect
Thermo Fisher Connect with cloud-enabled services provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within Thermo Fisher Connect are web browser-based software tools that are flexible, fast, and easy to use.

Easy to use
The QuantStudio 1 Real-Time PCR System is compact in form, factory calibrated, and delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience, and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software

Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through Thermo Fisher Connect
• No software to install, no licenses and no versions to update

Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 and Windows® 10 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology, which features three coupled excitation and emission filter channels with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

QuantStudio™ 12K Flex Real-Time PCR System, Fast 96-well block, laptop (Applied Biosystems™)

The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems™ real-time technology in a single instrument. The QuantStudio 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray™ plates, TaqMan™ array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it's now or in the future, for maximum flexibility and consistency in your research.

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

The QuantStudio 12K Flex System's intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

Flexibility & Scalability
Includes five interchangeable blocks: The QuantStudio 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan array cards, and OpenArray plates, for effortless scaling from 1 to 12,000 data points in a single run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan array cards and OpenArray plates.

• Compatible with a full range of TaqMan assays: The QuantStudio 12K Flex System is optimized to enable clear, clean data for all TaqMan assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.

• Provides a seamless switch from qPCR to digital PCR: Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio Digital PCR kits and DigitalSuite™ Software with the QuantStudio OpenArray block.

• Delivers peace of mind: Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.

Maximum Throughput
Enhanced OptiFlex™ System: Building upon Applied Biosystems technology, the Enhanced OptiFlex System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single run.

• Unprecedented coverage and sample throughput: The OpenArray format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.

• Simple OpenArray workflow: QuantStudio Open Array plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.

• Integrated analysis and quality systems: New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.

QuantStudio 12K Flex Software
• Intuitive interface and innovative design for novice and experienced users
• Convenient walk-away operation
• Improved data analysis across block types
• Fully compatible with high-throughput environments

Expandable Software Architecture
Optional add-ons are available to upgrade the existing software:
21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.

• High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.

• DigitalSuite Software v1.0: For OpenArray block users needing sensitivity for rare targets and precision to measure small differences in target abundance.

• ExpressionSuite Software v1.0: Stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray plates.

QuantStudio™ 1 Real-Time PCR System, 96-well, 0.2 mL, laptop (Applied Biosystems™)

The Applied Biosystems QuantStudio 1 Real-Time PCR System makes quality and reliability the practical choice. This affordable instrument offers basic features ideal for those new to qPCR or those who have a limited budget. Despite its affordability, the QuantStudio 1 system offers the quality, reliability, and advanced user experience of our QuantStudio family of instruments.

Download Quantstudio 1 Design & Analysis Software >   |   Release notes

Features of the QuantStudio 1 system include:
Touchscreen—a large 8.5-inch interactive touchscreen
Fixed block—one fixed 96-well, 0.2-mL isothermal block with an easy-to-open manual drawer
Reaction volume—10–100 μL
Multiplexing capability—offers the three most commonly used excitation and emission filters: FAM, VIC, and ROX
Protocols—pre-optimized protocol templates
Software—intuitive software that supports analysis via web browser or desktop computer
Dyes—three filters calibrated for FAM/SYBR Green/SYTO 9, VIC/HEX/TET/JOE, and JUN/ROX/Texas Red dyes
Sensitivity—detect differences as small as 1.5-fold quantities in singleplex reactions
Dimensions (H x W x D) and weight—40 x 27 x 50 cm, <26 kg

Thermo Fisher Connect
Thermo Fisher Connect with cloud-enabled services provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within Thermo Fisher Connect are web browser-based software tools that are flexible, fast, and easy to use.

Easy to use
The QuantStudio 1 Real-Time PCR System is compact in form, factory calibrated, and delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience, and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software

Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through Thermo Fisher Connect
• No software to install, no licenses and no versions to update

Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 and Windows® 10 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology, which features three coupled excitation and emission filter channels with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

QuantStudio™ 5 Real-Time PCR System, 96-well, 0.2 mL, desktop (Applied Biosystems™)

The Applied Biosystems® QuantStudio® 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio® 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 6 decoupled channels and white LED) and featuring six independent Veriflex® temperature zones, the QuantStudio® 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio® 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

Quantstudio 3/5 Firmware and Design & Analysis Software >

Features of the QuantStudio® 5 system with 96-well block include:

• Proven OptiFlex® technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Easy to use
The QuantStudio® 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio® Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio® Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio® 5 system offers users greater control of experiment data

Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex® technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

QuantStudio™ 7 Flex Real-Time PCR System, 96-well Fast, desktop (Applied Biosystems™)

The Applied Biosystems® QuantStudio™ 7 Flex Real-Time PCR System advances your research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, intuitive software, automation capabilities, and touch-screen interface, the QuantStudio™ 7 Flex system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation.

Features of the QuantStudio™ 7 Flex Real-Time PCR System include:

• Increased application versatility through the accomodation of 4 different block types
• Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
• The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
• Easy-to-use software, responsive touch-screen, automation capabilities, and effortless block exchange without the need for any tools

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

>> Download a 90-day trial of the QuantStudio™ 7 software

The QuantStudio™ 7 Flex system accommodates the interchange of a 96-well, 96-well Fast, 384-well, or TaqMan® array card block. The system is delivered with one block type (a 96-well Fast block in this case); other block types may be purchased separately. In addition, a Twister® Robot Automation Accessory is available for this system to support high throughput workflows. For a more value-priced system that does not support TaqMan® array cards or automation (but that can be upgraded to do so at a later date), please see our QuantStudio™ 6 Flex system. And for the ultimate in flexibility, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

Accomplish More, Faster
Run hundreds of real-time PCR reactions effortlessly using TaqMan® Array 384-well microfluidic cards. And when integrated with the Twister® Robot Automation Accessory (available separately), the QuantStudio™ 7 Flex system maximizes your throughput capabilities.

High Application Versatility
Simple workflows, reliable assays and reagents, multiple formats, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, High Resolution Melt (HRM), and Protein Thermal Shift™ experiments.

Results You Can Trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex™ system, which features 6 decoupled excitation and emission filter channels with 21 filter combinations for maximum multiplexing and chemistry flexibility.

Sensitive Data Analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

Precision Plate Holder for Plates, for 7500 Fast System (Applied Biosystems™)

Precision plate holder for 7500 fast system.

For Research Use Only. Not for use in diagnostics procedures.

QuantStudio™ 12K Flex Real-Time PCR System, 384-well block, laptop (Applied Biosystems™)

The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems™ real-time technology in a single instrument. The QuantStudio 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray™ plates, TaqMan™ array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it's now or in the future, for maximum flexibility and consistency in your research.

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

The QuantStudio 12K Flex System's intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

Flexibility & Scalability
Includes five interchangeable blocks: The QuantStudio 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan array cards, and OpenArray plates, for effortless scaling from 1 to 12,000 data points in a single run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan array cards and OpenArray plates.

• Compatible with a full range of TaqMan assays: The QuantStudio 12K Flex System is optimized to enable clear, clean data for all TaqMan assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.

• Provides a seamless switch from qPCR to digital PCR: Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio Digital PCR kits and DigitalSuite™ Software with the QuantStudio OpenArray block.

• Delivers peace of mind: Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.

Maximum Throughput
Enhanced OptiFlex™ System: Building upon Applied Biosystems technology, the Enhanced OptiFlex System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single run.

• Unprecedented coverage and sample throughput: The OpenArray format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.

• Simple OpenArray workflow: QuantStudio Open Array plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.

• Integrated analysis and quality systems: New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.

QuantStudio 12K Flex Software
• Intuitive interface and innovative design for novice and experienced users
• Convenient walk-away operation
• Improved data analysis across block types
• Fully compatible with high-throughput environments

Expandable Software Architecture
Optional add-ons are available to upgrade the existing software:
21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.

• High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.

• DigitalSuite Software v1.0: For OpenArray block users needing sensitivity for rare targets and precision to measure small differences in target abundance.

• ExpressionSuite Software v1.0: Stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray plates.

7500 Fast Precision Plate Holder, for 0.1 mL tube strips (Applied Biosystems™)

The Applied Biosystems™ 7500 Fast Precision Plate Holder contains one precision plate holder to be used with 0.1 ml tube strips on the 7500 Fast System, and the recommended usage procedure. Note: A29252 replaces Cat. No. 4403809.