Shop All Real-Time PCR Instruments & Accessories

QuantStudio™ 3 Real-Time PCR System, 96-well, 0.1 mL (Applied Biosystems™)

The Applied Biosystems® QuantStudio® 3 Real-Time PCR System is designed for users who need an affordable, easy-to-use real-time PCR system that doesn't compromise performance and quality. The simplified Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio® 3 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 4 coupled channels and white LED) and featuring three independent Veriflex® temperature zones, the QuantStudio® 3 system enables improved data accuracy and sensitivity for a broad range of genomic applications.

Quantstudio 3/5 Firmware and Design & Analysis Software >

Features of the QuantStudio® 3 system include:

• Simplified instrument software with interactive touchscreen for greater ease of use
• Proven OptiFlex® technology (4 coupled channels with white LED) for trusted results
• Secure cloud-based software option provides increased accessibility and storage options
• User account management, locked workflow, and Pause features for greater control of experiments
• 96-well 0.2 mL format or 96-well 0.1 mL format

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Easy to use
The QuantStudio® 3 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio® Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio® Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex® technology, which features 4 coupled excitation and emission filter channels with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

Precision Plate Holder for 7300 & 7500 Systems (Applied Biosystems™)

This precision plate holder is for use with Applied Biosystems® 7300 real-time PCR systems (SN 273000878 and above), as well as 7500 real-time PCR systems (SN 275001330 and above). Customers with earlier serial numbers should contact Product Support.

QuantStudio™ 7 Flex Real-Time PCR System, array card, desktop (Applied Biosystems™)

The Applied Biosystems® QuantStudio™ 7 Flex Real-Time PCR System advances your research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, intuitive software, automation capabilities, and touch-screen interface, the QuantStudio™ 7 Flex system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation.

Features of the QuantStudio™ 7 Flex Real-Time PCR System include:

• Increased application versatility through the accomodation of 4 different block types
• Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
• The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
• Easy-to-use software, responsive touch-screen, automation capabilities, and effortless block exchange without the need for any tools

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

>> Download a 90-day trial of the QuantStudio™ 7 software

The QuantStudio™ 7 Flex system accommodates the interchange of a 96-well, 96-well Fast, 384-well, or TaqMan® array card block. The system is delivered with one block type (an array card block in this case); other block types may be purchased separately. In addition, a Twister® Robot Automation Accessory is available for this system to support high throughput workflows. For a more value-priced system that does not support TaqMan® array cards or automation (but that can be upgraded to do so at a later date), please see our QuantStudio™ 6 Flex system. And for the ultimate in flexibility, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

Accomplish More, Faster
Run hundreds of real-time PCR reactions effortlessly using TaqMan® Array 384-well microfluidic cards. And when integrated with the Twister® Robot Automation Accessory (available separately), the QuantStudio™ 7 Flex system maximizes your throughput capabilities.

High Application Versatility
Simple workflows, reliable assays and reagents, multiple formats, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, High Resolution Melt (HRM), and Protein Thermal Shift™ experiments.

Results You Can Trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex™ system, which features 6 decoupled excitation and emission filter channels with 21 filter combinations for maximum multiplexing and chemistry flexibility.

Sensitive Data Analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

QuantStudio™ 3 Real-Time PCR System, 96-well, 0.2 mL (Applied Biosystems™)

The Applied Biosystems® QuantStudio® 3 Real-Time PCR System is designed for users who need an affordable, easy-to-use real-time PCR system that doesn't compromise performance and quality. The simplified Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio® 3 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 4 coupled channels and white LED) and featuring three independent Veriflex® temperature zones, the QuantStudio® 3 system enables improved data accuracy and sensitivity for a broad range of genomic applications.

Quantstudio 3/5 Firmware and Design & Analysis Software >

Features of the QuantStudio® 3 system include:

• Simplified instrument software with interactive touchscreen for greater ease of use
• Proven OptiFlex® technology (4 coupled channels with white LED) for trusted results
• Secure cloud-based software option provides increased accessibility and storage options
• User account management, locked workflow, and Pause features for greater control of experiments
• 96-well 0.2 mL format or 96-well 0.1 mL format

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Easy to use
The QuantStudio® 3 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio® Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio® Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex® technology, which features 4 coupled excitation and emission filter channels with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

SureTect™ Real-Time PCR System (Thermo Scientific™)

Quickly and accurately detect microorganisms in a broad range of food and environmental samples using the Thermo Scientific™ SureTect™ System.

Precision Plate Holder for 0.2 mL Tubes & Strips (for 7300 & 7500 Systems) (Applied Biosystems™)

This precision plate holder is for use with 0.2 mL tube strips on the Applied Biosystems® 7300 and 7500 Real-Time PCR systems.

StepOnePlus™ Real-Time PCR System Upgrade (Applied Biosystems™)

This kit upgrades a 48-well StepOne™ Real-Time PCR System to a 96-well system.

For Research Use Only. Not for use in diagnostics procedures.

QuantStudio™ 12K Flex OpenArray™ Accessories Kit (Applied Biosystems™)

The QuantStudio™12K Flex OpenArray® Accessories Kit contains the items needed to assemble up to 10 QuantStudio™ OpenArray® plates. These items include 12 lids and plugs, 12 immersion fluid syringes, and 2 carriers.

QuantStudio™ 6 Flex Real-Time PCR System, 96-well Fast, desktop (Applied Biosystems™)

The Applied Biosystems® QuantStudio™ 6 Flex Real-Time PCR System facilitates real-time PCR based applications with an upgradable block selection, allowing you to plan for experiments today and grow with tomorrow’s needs. With a simplified workflow, intuitive software, and touch-screen interface, the QuantStudio™ 6 Flex system offers exceptional reproducibility at a price you can afford.

Features of the QuantStudio™ 6 Flex Real-Time PCR System include:

• Affordable price allows you to obtain a high performance instrument with minimal upfront capital investment
• Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
• The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
• Easy-to-use software, responsive touch-screen, and effortless block exchange without the need for any tools

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

>> Download a 90-day trial of the QuantStudio™ 6 software

The QuantStudio™ 6 Flex system accommodates the interchange of a 96-well, 96-well Fast, or 384-well block. The system is delivered with one block type (a 96-well Fast block in this case); other block types may be purchased separately. For a system that also supports TaqMan® array cards, as well as automation, please see our QuantStudio™ 7 Flex system. The QuantStudio™ 6 Flex system can also be upgraded to a QuantStudio™ 7 Flex system at a later date, as your needs change. For the ultimate in productivity, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

Upgrade Capabilities When You Need Them

The QuantStudio™ 6 Flex system can be seamlessly upgraded to a QuantStudio™ 7 Flex system by a field service engineer to give you additional automation, throughput, and multiplexing capabilities.

Results You Can Trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex® system, which features 5 coupled excitation and emission filter channels.

Sensitive Data Analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

StepOnePlus™ Real-Time PCR System (Applied Biosystems™)

The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

Features of the StepOnePlus™ Real-Time PCR System include:

• Advanced software and instrumentation for performing a wide array of genomic assays

• Sensitive 4-color optical LED recording system

• Intuitive and robust software perfect for both first-time and advanced users

• Simple and flexible instrument set-up and usage

New StepOne™ Software

The StepOne™ Software included with the StepOnePlus™ Real-Time PCR System runs on the Windows® XP operating system and provides instrument control, data collection, and data analysis capabilities (analysis software is also compatible with Windows® 7). This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.

Rapid and Simple Assay Set-Up

This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOnePlus™ Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.

Sensitive LED-Based 4 Color Fluorescence Reading

The StepOnePlus™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, NED™/TAMRA™, and ROX™ dyes. This cost-effective, 4-color, 96-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.

Compatible with Many Genomic Analysis Techniques

Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus™ Real-Time PCR System and StepOne™ Software.

The StepOnePlus™ System supports many Real-Time PCR applications, including the following:

• SNP Genotyping

• Gene Expression Analysis

• MicroRNA Expression

• Protein Expression

• Translocation Analysis

• Gene Detection

• Viral Load Analysis

The included StepOne™ Software supports a variety of analysis methods, including the following:

• Standard Curve (absolute quantitation)

• Relative Standard Curve

• Comparative Ct (ΔΔCt) (relative quantitation)

• Genotyping and Presence/Absence

• Melt Curve Analysis (as a standalone application)

High Resolution Melting (as a standalone application)

Versatile Instrument Configuration Options

The ultra-compact footprint of a StepOnePlus™ System can be installed in multiple distinct configurations, providing unmatched flexibility and convenience that can allow a fit in any laboratory (Fig 2). The StepOnePlus™ Real-Time PCR System can be used via a PC, with a PC connected to a Local Area Network (LAN), or as a stand-alone instrument (PC-free). Each StepOnePlus™ Real-Time PCR System is factory-calibrated for optical and thermal accuracy, so simply remarkable Real-Time PCR results can be obtained right out of the box.

Energy Efficiency and Space Saving

The StepOnePlus™ Real-Time PCR System draws 38% less energy to process one sample plate (when the instrument is in a heated state), compared to the Bio-Rad CFX96™ Real-Time PCR Detection System. The StepOnePlus™ Real-Time PCR System also has advanced temperature control through use of VeriFlex™ Blocks technology and has a footprint nearly 15% smaller than Bio-Rad CFX96™, which in today’s crowded laboratories helps you use your laboratory space even more efficiently. You also leave a smaller environmental footprint on the Earth.

Real-Time Data Monitoring, Dissemination, and Storage

The system measures amplification as it occurs, allowing you to monitor the progress of your experiment cycle by cycle, either on the machine or remotely. Your data is stored on the instrument itself, and can be viewed and stored automatically via remote access, or transferred via email or a USB flash drive. Data can also be conveniently exported as PowerPoint®, Excel®, and graphical image files.

Expanded Gene Expression Capabilities

The advanced software provided with the StepOnePlus™ Real-Time PCR System now includes the powerful Gene Expression Study Package. This software package allows for greater flexibility and accuracy in your gene expression assays through:

• Analysis of an unlimited number of plates in one study

• Sorting of data by biological or technical replicate group

• Use of multiple endogenous controls

• Assay efficiency correction

Advanced High Resolution Melting Capability

Now with High Resolution Melt Software v3.0, you can perform post-PCR sequence variation analysis with the StepOnePlus™ Real-Time PCR System. This separately purchased software simplifies set-up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel. HRM Software v3.0 also has an improved clustering algorithm for increased sensitivity and accuracy, and the ability to conduct separate analyses of multiple assays run on a single plate.

Twister™ Automation Robot for QuantStudio™ Systems (Applied Biosystems™)

The Twister® Automation Robot for QuantStudio™ Systems is designed for use with the QuantStudio™ 12K Flex and QuantStudio™ 7 Flex real-time PCR systems, enabling real-time PCR in automated environments for increased sample throughput. This robot provides extended hours of walk-away automation so you can make better use of your research time.

Highlights:

• Runs up to 102 individual 384-well reaction plates, 102 TaqMan® Array microfluidic cards, 48 96-well plates, or 62 Fast 96-well plates
• Bench-top system with a compact footprint
• Removable stacks offer effortless expansion or contraction so the system does not takes up more valuable lab space than necessary

7500 Fast System Upgrade Kit (Applied Biosystems™)

The Applied Biosystems® 7500 Fast Real-Time PCR System enables standard 96-well format high speed thermal cycling, significantly reducing your run time for quantitative real-time PCR applications, delivering results in about 30 minutes. The Upgrade Kit is available to upgrade a standard 7500 Real-Time PCR System to the Fast Configuration via a field service installation.

For Research Use Only. Not for use in diagnostics procedures.

Pharmaceutical Analytics QuantStudio™ 5 Real-Time PCR System, 384-well, desktop (Applied Biosystems™)

The Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System for Pharmaceutical Analytics is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The QuantStudio 5 pharmaceutical analytics system is used together with specialized sample preparation and quantitation kits for host cell protein, host cell DNA, and Protein A impurity testing of biological products produced in cell culture.The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 6 decoupled channels and white LED) and featuring six independent Veriflex® temperature zones, the QuantStudio 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

Features of the QuantStudio 5 system with 96-well block include:

• Proven OptiFlex technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Downloads
>> QuantStudio 3/5 Design & Analysis Software | Release Notes | Example Data & Templates
>> QuantStudio 3/5 Firmware | Release Notes

Easy to use
The QuantStudio 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio 5 system offers users greater control of experiment data

Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

QuantStudio™ 5 Real-Time PCR System, 96-well, 0.1 mL (Applied Biosystems™)

The Applied Biosystems® QuantStudio® 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio® 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 6 decoupled channels and white LED) and featuring six independent Veriflex® temperature zones, the QuantStudio® 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio® 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

Quantstudio 3/5 Firmware and Design & Analysis Software >

Features of the QuantStudio® 5 system with 96-well block include:

• Proven OptiFlex® technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Easy to use
The QuantStudio® 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio® Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio® Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio® 5 system offers users greater control of experiment data

Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex® technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

Replacement Lamp for ViiA™ 7 Real-Time PCR System (Applied Biosystems™)

Replacement lamp, light source, for the ViiA™ 7 Instrument. Calibration is recommended after lamp replacement.