Shop All Real-Time PCR Instruments & Accessories

Precision Plate Holder for 0.2 mL Tubes & Strips (for 7300 & 7500 Systems) (Applied Biosystems™)

This precision plate holder is for use with 0.2 mL tube strips on the Applied Biosystems® 7300 and 7500 Real-Time PCR systems.

StepOnePlus™ Real-Time PCR System Upgrade (Applied Biosystems™)

This kit upgrades a 48-well StepOne™ Real-Time PCR System to a 96-well system.

For Research Use Only. Not for use in diagnostics procedures.

7900HT Fast Real-Time Service Upgrade (Applied Biosystems™)

The 7900HT Fast Real-Time Service Upgrade allows existing 7900HT customers to upgrade their system for use with Fast thermal cycling runs. This item should only be ordered if the customer has a GX270 (or higher) computer. Customers with older computers must order the 7900HT Fast Real-Time Service Upgrade with Computer (4362078).

The 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability.

• Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate.
• Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction.
• Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences.
• 96- or 384-well plate compatibility (including the TaqMan® Low Density Array).
• Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security.
• Hands-free plate-loading and unloading provides true walkaway automation allowing you to increase your lab's productivity.
• Proven assay development guidelines save time and money

High Throughput
The 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. An Automation Accessory combined with 384-well plate capability make the 7900HT system ideally suited to meet the high-throughput requirements of today's drug discovery process. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay.

Flexibility
The 7900HT system is a versatile research tool that can accommodate any real-time PCR need. User-interchangeable thermal cycling block formats let you select the format that’s right for your project, using industry-standard 96- and 384-well formats, as well as a novel 384-well TaqMan® Low Density Array and a new Fast 96-well block that reduces run times from 2 hours to about 30 minutes. An easy automation upgrade path lets you add features to meet throughput demands. A Fast PCR option reduces run times from 2 hours to about 30 minutes.

Powerful Software
Using two automation software tools, Plate Utility and Automation Controller, most of the gene expression and SNP genotyping workflow is fully automated for high-throughput applications and requires minimal user intervention. You can now process 5,000 x 384 well plates (384 markers), which equates to 1.92 million genotypes. Using Relative Quantification (RQ) Manager, you can also process 200 x 384-well plates (96 detectors with quadruplicate data points⁄multiplexed endogenous control), which equates to 153,600 data points. Fully integrated into one complete Enterprise system, SNP Manager and RQ Manager eliminate data analysis bottlenecks in high-throughput SNP and gene expression research by allowing significantly more plates to be analyzed simultaneously. This reduces hands-on analysis time and simplifies the data analysis workflow.

For Research Use Only. Not for use in diagnostics procedures.

QuantStudio™ 7 Flex Real-Time PCR System, 384-well, desktop (Applied Biosystems™)

The Applied Biosystems® QuantStudio™ 7 Flex Real-Time PCR System advances your research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, intuitive software, automation capabilities, and touch-screen interface, the QuantStudio™ 7 Flex system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation.

Features of the QuantStudio™ 7 Flex Real-Time PCR System include:

• Increased application versatility through the accomodation of 4 different block types
• Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
• The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
• Easy-to-use software, responsive touch-screen, automation capabilities, and effortless block exchange without the need for any tools

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

>> Download a 90-day trial of the QuantStudio™ 7 software

The QuantStudio™ 7 Flex system accommodates the interchange of a 96-well, 96-well Fast, 384-well, or TaqMan® array card block. The system is delivered with one block type (a 384-well block in this case); other block types may be purchased separately. In addition, a Twister® Robot Automation Accessory is available for this system to support high throughput workflows. For a more value-priced system that does not support TaqMan® array cards or automation (but that can be upgraded to do so at a later date), please see our QuantStudio™ 6 Flex system. And for the ultimate in flexibility, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

Accomplish More, Faster
Run hundreds of real-time PCR reactions effortlessly using TaqMan® Array 384-well microfluidic cards. And when integrated with the Twister® Robot Automation Accessory (available separately), the QuantStudio™ 7 Flex system maximizes your throughput capabilities.

High Application Versatility
Simple workflows, reliable assays and reagents, multiple formats, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, High Resolution Melt (HRM), and Protein Thermal Shift™ experiments.

Results You Can Trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex™ system, which features 6 decoupled excitation and emission filter channels with 21 filter combinations for maximum multiplexing and chemistry flexibility.

Sensitive Data Analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

PikoReal 96, validation plate (1 plate) (Thermo Scientific™)

Thermo Scientific PikoReal 96 Validation Plate is designed to verify the performance of the Thermo Scientific PikoReal 96 RealTime PCR System.

The Validation Plate is a pre-loaded and sealed 96-well plate. Each well contains 10 µl of reaction mixture with the same quantity of Lambda DNA template in each reaction. The qPCR mixture of the PikoReal 96 Validation Plate is green to visualize the reaction mixture in each well and evaluate equal volume in each well before and after cycling.

Applied Biosystems™ 7500 Fast Dx Real-Time PCR Instrument, with laptop computer (Applied Biosystems™)

The Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument with SDS Software is a real-time nucleic acid amplification and five-color fluorescence detection system available for in vitro diagnostic use. The 7500 Fast Dx Real-Time PCR Instrument delivers the performance required for high-quality results in a 96-well format.

• 96-well format eases plate setup if automation is not available
• Accommodates tube strips, which can be capped immediately after pipetting each sample
• Fast mode completes runs in less than forty minutes
• Optional standard mode facilitates use of standard length real-time PCR assays without changing thermal cycling parameters
• 5-color variable excitation enables multiplex assays

Convenient 96-well Format
The 7500 Fast Dx Real-Time PCR Instrument minimizes the effort required to perform sample setup while also allowing the use of tube strips.

Intuitive Software to Run Pre-optimized Protocols
The 7500 Fast Dx instrument's Sequence Detection Software Security, Auditing, and E-Signature module offers the flexibility to develop assays and also run pre-optimized protocols for users operating in a secure environment.

Instrument Service and Support
The 7500 Fast Dx Real-Time PCR Instrument is supported by our AB Assurance Service Plan for Diagnostics, which includes:
• Instrument Installation Qualification, Operation Qualification, and Performance Qualification (IQ⁄OQ⁄PQ)
• Spectral calibration and software setup
• Emergency repair, including parts and labor, and OQ⁄PQ after any major part change
• Planned maintenance every 12 months

Europe: For in vitro Diagnostic Use. The 7500 Fast Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98⁄79⁄EC). The CE IVD-registered 7500 Fast Dx Real-Time PCR Instrument is for distribution and use in specific European countries only.

USA: For in vitro Diagnostic Use. The 7500 Fast Dx Real-Time PCR instrument is 510(k)-cleared (K082562). The customer is responsible for any validation of assays and compliance with any regulatory requirements that pertain to their procedures and instrument use.

Tool, 5/16 HEX 6" L W/T-handle (Applied Biosystems™)

This is a tool for use with the 7900HT Sequence Detection System.

For Research Use Only. Not for use in diagnostics procedures.

Pharmaceutical Analytics QuantStudio™ 5 Real-Time PCR System, 384-well (Applied Biosystems™)

The Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System for Pharmaceutical Analytics is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The QuantStudio 5 pharmaceutical analytics system is used together with specialized sample preparation and quantitation kits for host cell protein, host cell DNA, and Protein A impurity testing of biological products produced in cell culture.The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 6 decoupled channels and white LED) and featuring six independent Veriflex® temperature zones, the QuantStudio 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

Features of the QuantStudio 5 system with 96-well block include:

• Proven OptiFlex technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Downloads
>> QuantStudio 3/5 Design & Analysis Software | Release Notes | Example Data & Templates
>> QuantStudio 3/5 Firmware | Release Notes

Easy to use
The QuantStudio 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio 5 system offers users greater control of experiment data

Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

QuantStudio™ 3D Digital PCR Spares Kit v2 (Applied Biosystems™)

The Applied Biosystems™ QuantStudio™ 3D Digital PCR Spares Kit v2 contains Chip Lids v2 and Blades used to securely cover and seal QuantStudio 3D Digital PCR Chips v2 after reagent addition. Simply load the reaction mix onto a Chip v2 using a blade, cover the chip with a Chip Lid v2, and seal the chip. The chip is then ready for amplification and analysis.

QuantStudio 3D Digital PCR Spares Kit v2 is designed to deliver accurate, sensitive, and precise digital PCR data on QuantStudio 3D Digital PCR instruments when combined with associated consumables, reagents, and QuantStudio™ 3D Analysis Suite™ software.

Precision Plate Holder for 7500 Real-Time PCR Systems (Applied Biosystems™)

This precision plate holder is required for use of MicroAmp® EnduraPlate™ optical reaction plates on Applied Biosystems® 7500 real-time PCR systems. It also acommodates MicroAmp® optical reaction plates.

StepOnePlus™ Real-Time PCR System, laptop (Applied Biosystems™)

The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications. We supply a Dell™ Laptop with the StepOnePlus™ Real-Time PCR System.

Features of the StepOnePlus™ Real-Time PCR System include:

• Advanced software and instrumentation for performing a wide array of genomic assays
• Sensitive 4-color optical LED recording system
• Intuitive and robust software perfect for both first-time and advanced users
• Simple and flexible instrument set-up and usage

New StepOne™ Software
The StepOne™ Software included with the StepOnePlus™ Real-Time PCR System runs on the Windows® XP operating system and provides instrument control, data collection, and data analysis capabilities (analysis software is also compatible with Windows® 7). This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.

Rapid and Simple Assay Set-Up
This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOnePlus™ Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.

Sensitive LED-Based 4 Color Fluorescence Reading
The StepOnePlus™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, NED™/TAMRA™, and ROX™ dyes. This cost-effective, 4-color, 96-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.

Compatible with Many Genomic Analysis Techniques
Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus™ Real-Time PCR System and StepOne™ Software.

The StepOnePlus™ System supports many Real-Time PCR applications, including the following:

• SNP Genotyping
• Gene Expression Analysis
• MicroRNA Expression
• Protein Expression
• Translocation Analysis
• Gene Detection
• Viral Load Analysis

The included StepOne™ Software supports a variety of analysis methods, including the following:

• Standard Curve (absolute quantitation)
• Relative Standard Curve
• Comparative Ct (ΔΔCt) (relative quantitation)
• Genotyping and Presence/Absence
• Melt Curve Analysis (as a standalone application)
High Resolution Melting (as a standalone application)

Versatile Instrument Configuration Options
The ultra-compact footprint of a StepOnePlus™ System can be installed in multiple distinct configurations, providing unmatched flexibility and convenience that can allow a fit in any laboratory (Fig 2). The StepOnePlus™ Real-Time PCR System can be used via a PC, with a PC connected to a Local Area Network (LAN), or as a stand-alone instrument (PC-free). Each StepOnePlus™ Real-Time PCR System is factory-calibrated for optical and thermal accuracy, so simply remarkable Real-Time PCR results can be obtained right out of the box.

Energy Efficiency and Space Saving
The StepOnePlus™ Real-Time PCR System draws 38% less energy to process one sample plate (when the instrument is in a heated state), compared to the Bio-Rad CFX96™ Real-Time PCR Detection System. The StepOnePlus™ Real-Time PCR System also has advanced temperature control through use of VeriFlex™ Blocks technology and has a footprint nearly 15% smaller than Bio-Rad CFX96™, which in today’s crowded laboratories helps you use your laboratory space even more efficiently. You also leave a smaller environmental footprint on the Earth.

Real-Time Data Monitoring, Dissemination, and Storage
The system measures amplification as it occurs, allowing you to monitor the progress of your experiment cycle by cycle, either on the machine or remotely. Your data is stored on the instrument itself, and can be viewed and stored automatically via remote access, or transferred via email or a USB flash drive. Data can also be conveniently exported as PowerPoint®, Excel®, and graphical image files.

Expanded Gene Expression Capabilities
The advanced software provided with the StepOnePlus™ Real-Time PCR System now includes the powerful Gene Expression Study Package. This software package allows for greater flexibility and accuracy in your gene expression assays through:

• Analysis of an unlimited number of plates in one study
• Sorting of data by biological or technical replicate group
• Use of multiple endogenous controls
• Assay efficiency correction

Advanced High Resolution Melting Capability
Now with High Resolution Melt Software v3.0, you can perform post-PCR sequence variation analysis with the StepOnePlus™ Real-Time PCR System. This separately purchased software simplifies set-up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel. HRM Software v3.0 also has an improved clustering algorithm for increased sensitivity and accuracy, and the ability to conduct separate analyses of multiple assays run on a single plate.

Applied Biosystems™ 7500 Fast Dx Real-Time PCR Instrument, with tower computer (Applied Biosystems™)

The Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument with SDS Software is a real-time nucleic acid amplification and five-color fluorescence detection system available for in vitro diagnostic use. The 7500 Fast Dx Real-Time PCR Instrument delivers the performance required for high-quality results in a 96-well format.

• 96-well format eases plate setup if automation is not available
• Accommodates tube strips, which can be capped immediately after pipetting each sample
• Fast mode completes runs in less than forty minutes
• Optional standard mode facilitates use of standard length real-time PCR assays without changing thermal cycling parameters
• 5-color variable excitation enables multiplex assays

Convenient 96-well Format
The 7500 Fast Dx Real-Time PCR Instrument minimizes the effort required to perform sample setup while also allowing the use of tube strips.

Intuitive Software to Run Pre-optimized Protocols
The 7500 Fast Dx instrument's Sequence Detection Software Security, Auditing, and E-Signature module offers the flexibility to develop assays and also run pre-optimized protocols for users operating in a secure environment.

Instrument Service and Support
The 7500 Fast Dx Real-Time PCR Instrument is supported by our AB Assurance Service Plan for Diagnostics, which includes:
• Instrument Installation Qualification, Operation Qualification, and Performance Qualification (IQ⁄OQ⁄PQ)
• Spectral calibration and software setup
• Emergency repair, including parts and labor, and OQ⁄PQ after any major part change
• Planned maintenance every 12 months

Europe: For in vitro Diagnostic Use. The 7500 Fast Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98⁄79⁄EC). The CE IVD-registered 7500 Fast Dx Real-Time PCR Instrument is for distribution and use in specific European countries only.

USA: For in vitro Diagnostic Use. The 7500 Fast Dx Real-Time PCR instrument is 510(k)-cleared (K082562). The customer is responsible for any validation of assays and compliance with any regulatory requirements that pertain to their procedures and instrument use.

Pharmaceutical Analytics QuantStudio™ 5 Real-Time PCR System, 384-well, laptop (Applied Biosystems™)

The Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System for Pharmaceutical Analytics is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The QuantStudio 5 pharmaceutical analytics system is used together with specialized sample preparation and quantitation kits for host cell protein, host cell DNA, and Protein A impurity testing of biological products produced in cell culture.The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 6 decoupled channels and white LED) and featuring six independent Veriflex® temperature zones, the QuantStudio 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

Features of the QuantStudio 5 system with 96-well block include:

• Proven OptiFlex technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Downloads
>> QuantStudio 3/5 Design & Analysis Software | Release Notes | Example Data & Templates
>> QuantStudio 3/5 Firmware | Release Notes

Easy to use
The QuantStudio 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio 5 system offers users greater control of experiment data

Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

QuantStudio™ 12K Flex Real-Time PCR System, Array Card block, laptop (Applied Biosystems™)

The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems™ real-time technology in a single instrument. The QuantStudio 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray™ plates, TaqMan™ array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it's now or in the future, for maximum flexibility and consistency in your research.

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

The QuantStudio 12K Flex System's intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

Flexibility & Scalability
Includes five interchangeable blocks: The QuantStudio 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan array cards, and OpenArray plates, for effortless scaling from 1 to 12,000 data points in a single run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan array cards and OpenArray plates.

• Compatible with a full range of TaqMan assays: The QuantStudio 12K Flex System is optimized to enable clear, clean data for all TaqMan assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.

• Provides a seamless switch from qPCR to digital PCR: Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio Digital PCR kits and DigitalSuite™ Software with the QuantStudio OpenArray block.

• Delivers peace of mind: Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.

Maximum Throughput
Enhanced OptiFlex™ System: Building upon Applied Biosystems technology, the Enhanced OptiFlex System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single run.

• Unprecedented coverage and sample throughput: The OpenArray format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.

• Simple OpenArray workflow: QuantStudio Open Array plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.

• Integrated analysis and quality systems: New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.

QuantStudio 12K Flex Software
• Intuitive interface and innovative design for novice and experienced users
• Convenient walk-away operation
• Improved data analysis across block types
• Fully compatible with high-throughput environments

Expandable Software Architecture
Optional add-ons are available to upgrade the existing software:
21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.

• High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.

• DigitalSuite Software v1.0: For OpenArray block users needing sensitivity for rare targets and precision to measure small differences in target abundance.

• ExpressionSuite Software v1.0: Stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray plates.

QuantStudio™ 3D Digital PCR Tilt Base for ProFlex™ Thermal Cycler (Applied Biosystems™)

The QuantStudio™ 3D Digital PCR Tilt Base for ProFlex™ Thermal Cycler sits beneath the ProFlex™ Thermal Cycler and ensures consistent performance during PCR amplification of the QuantStudio™ 3D Digital PCR chips. Simply affix the two risers and place the tilt base beneath the feet of the thermal cycler, load your prepared 3D Digital PCR chips, and you are ready for PCR amplification.