Shop All Real-Time PCR Instruments

QuantStudio™ 12K Flex Real-Time PCR System, OpenArray™ block, desktop (Applied Biosystems™)

The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The QuantStudio™ 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray® plates, TaqMan® array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it’s now or in the future, for maximum flexibility and consistency in your research. Note: this particular system includes an OpenArray block but does not include an AccuFill™ sample handling system. For a package that includes an AccuFill system, please see Cat. No. 4471090.

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

The QuantStudio™ 12K Flex System’s intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray® components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

Flexibility & Scalability
Includes five interchangeable blocks: The QuantStudio™ 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan® array cards, and OpenArray® plates, for effortless scaling from 1 to 12,000 data points in a single 100 minute run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan® array cards and OpenArray® plates.
Compatible with a full range of TaqMan® assays: The QuantStudio™ 12K Flex System is optimized to enable clear, clean data for all TaqMan® assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.
Provides a seamless switch from qPCR to digital PCR: Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio® Digital PCR kits and DigitalSuite Software with the QuantStudio™ OpenArray® block.
Delivers peace of mind: Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.

Horsepower
Enhanced OptiFlex™ System: Building upon Applied Biosystems’ proprietary technology, the Enhanced OptiFlex™ System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single 100 minute run.
Unprecedented coverage and sample throughput: The OpenArray® format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.
Simple OpenArray® workflow: QuantStudio™ Open Array® plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio™ 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.
Tools to maximize your resources: New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.

QuantStudio™ 12K Flex Software
• Intuitive interface and innovative design for novice and experienced users
• Convenient walk-away operation
• Improved data analysis across block types
• Fully compatible with high-throughput environments

Expandable Software Architecture
Optional add-ons are available to upgrade the existing software:
21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.
High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.
DigitalSuite Software v1.0: For OpenArray™ block users needing sensitivity for rare targets and precision to measure small differences in target abundance.
ExpressionSuite software v1.0: our latest stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray® plates.

QuantStudio™ 7 Flex Real-Time PCR System, 96-well, laptop (Applied Biosystems™)

The Applied Biosystems® QuantStudio™ 7 Flex Real-Time PCR System advances your research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, intuitive software, automation capabilities, and touch-screen interface, the QuantStudio™ 7 Flex system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation.

Features of the QuantStudio™ 7 Flex Real-Time PCR System include:

• Increased application versatility through the accomodation of 4 different block types
• Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
• The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
• Easy-to-use software, responsive touch-screen, automation capabilities, and effortless block exchange without the need for any tools

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

>> Download a 90-day trial of the QuantStudio™ 7 software

The QuantStudio™ 7 Flex system accommodates the interchange of a 96-well, 96-well Fast, 384-well, or TaqMan® array card block. The system is delivered with one block type (a 96-well block in this case); other block types may be purchased separately. In addition, a Twister® Robot Automation Accessory is available for this system to support high throughput workflows. For a more value-priced system that does not support TaqMan® array cards or automation (but that can be upgraded to do so at a later date), please see our QuantStudio™ 6 Flex system. And for the ultimate in flexibility, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

Accomplish More, Faster
Run hundreds of real-time PCR reactions effortlessly using TaqMan® Array 384-well microfluidic cards. And when integrated with the Twister® Robot Automation Accessory (available separately), the QuantStudio™ 7 Flex system maximizes your throughput capabilities.

High Application Versatility
Simple workflows, reliable assays and reagents, multiple formats, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, High Resolution Melt (HRM), and Protein Thermal Shift™ experiments.

Results You Can Trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex™ system, which features 6 decoupled excitation and emission filter channels with 21 filter combinations for maximum multiplexing and chemistry flexibility.

Sensitive Data Analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

QuantStudio™ Dx Real-Time PCR Instrument with 96-well fast block (Applied Biosystems™)

The QuantStudio™ Dx Real-Time PCR Instrument is a real-time PCR platform that delivers the proven performance, flexibility, expandability, and support needed to meet the evolving needs of the clinical laboratory today and tomorrow. The QuantStudio™ Dx instrument is approved for in vitro diagnostic (IVD) use with the Quidel® Molecular Direct C. difficile Assay, the first available from a growing menu of assays that are under development. In Test Development Mode, the QuantStudio™ Dx Real-Time PCR Instrument offers easy-to-change blocks that can also accommodate array cards, as well as 96-well standard, 96-well Fast, and 384-well plates, allowing you to select the format that is right for your throughput needs.

Built on a legacy of innovation and excellence in real-time PCR, the QuantStudio™ Dx instrument leverages the best of our portfolio of Applied Biosystems® real-time PCR systems, and refines a platform to specifically meet the needs of the diagnostic laboratory.

Results You Can Trust
The QuantStudio™ Dx Real-Time PCR Instrument's proven performance, security, and reliability—combined with the outstanding sensitivity and specificity of the Quidel® Molecular Assays—help ensure the right answer is delivered the first time, every time.

Flexibility You Want, Security You Need
In Test Development Mode, The QuantStudio™ Dx Real-Time PCR Instrument with the 96-Well fast Block is designed with easily interchangeable thermal cycling blocks to accommodate your laboratory's varying needs for throughput, format type, and assay type. In addition to the 96-well fast format that was cleared for use in the US with the Quidel Molecular Direct C. difficile Assay, the instrument also accepts 96-well standard, 384-well, and array card formats that can be used in Test Development Mode for assay development.

Efficient Workflows and Assays
A simplified instrument and assay workflow, as well as intuitive software, make the entire test easy and efficient, from processing to reporting. Plus, it frees up highly trained operators to perform more complex tasks.

QuantStudio™ Dx Software
• Track reagent and patient information for quality control purposes
• Enter reagent information by barcode and upload patient information from Excel files to minimize data entry errors
• Print calibration and maintenance reports for your instrument records
• Included SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability as part of 21 CRF Part 11 compliance

For In Vitro Diagnostic Use. The QuantStudio™ Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD-registered QuantStudio™ Dx Real-Time PCR Instrument is for distribution and use in specific countries only.

QuantStudio™ 5 Real-Time PCR System, 96-well, 0.1 mL (Applied Biosystems™)

The Applied Biosystems® QuantStudio® 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio® 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 6 decoupled channels and white LED) and featuring six independent Veriflex® temperature zones, the QuantStudio® 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio® 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

Quantstudio 3/5 Firmware and Design & Analysis Software >

Features of the QuantStudio® 5 system with 96-well block include:

• Proven OptiFlex® technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Easy to use
The QuantStudio® 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio® Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio® Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio® 5 system offers users greater control of experiment data

Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex® technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

QuantStudio™ 6 Flex Real-Time PCR System, 96-well, laptop (Applied Biosystems™)

The Applied Biosystems® QuantStudio™ 6 Flex Real-Time PCR System facilitates real-time PCR based applications with an upgradable block selection, allowing you to plan for experiments today and grow with tomorrow’s needs. With a simplified workflow, intuitive software, and touch-screen interface, the QuantStudio™ 6 Flex system offers exceptional reproducibility at a price you can afford.

Features of the QuantStudio™ 6 Flex Real-Time PCR System include:

• Affordable price allows you to obtain a high performance instrument with minimal upfront capital investment
• Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
• The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
• Easy-to-use software, responsive touch-screen, and effortless block exchange without the need for any tools

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

>> Download a 90-day trial of the QuantStudio™ 6 software

The QuantStudio™ 6 Flex system accommodates the interchange of a 96-well, 96-well Fast, or 384-well block. The system is delivered with one block type (a 96-well block in this case); other block types may be purchased separately. For a system that also supports TaqMan® array cards, as well as automation, please see our QuantStudio™ 7 Flex system. The QuantStudio™ 6 Flex system can also be upgraded to a QuantStudio™ 7 Flex system at a later date, as your needs change. For the ultimate in productivity, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

Upgrade Capabilities When You Need Them

The QuantStudio™ 6 Flex system can be seamlessly upgraded to a QuantStudio™ 7 Flex system by a field service engineer to give you additional automation, throughput, and multiplexing capabilities.

Results You Can Trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex® system, which features 5 coupled excitation and emission filter channels.

Sensitive Data Analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

QuantStudio™ 6 Flex Real-Time PCR System, 96-well, desktop (Applied Biosystems™)

The Applied Biosystems® QuantStudio™ 6 Flex Real-Time PCR System facilitates real-time PCR based applications with an upgradable block selection, allowing you to plan for experiments today and grow with tomorrow’s needs. With a simplified workflow, intuitive software, and touch-screen interface, the QuantStudio™ 6 Flex system offers exceptional reproducibility at a price you can afford.

Features of the QuantStudio™ 6 Flex Real-Time PCR System include:

• Affordable price allows you to obtain a high performance instrument with minimal upfront capital investment
• Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
• The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
• Easy-to-use software, responsive touch-screen, and effortless block exchange without the need for any tools

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

>> Download a 90-day trial of the QuantStudio™ 6 software

The QuantStudio™ 6 Flex system accommodates the interchange of a 96-well, 96-well Fast, or 384-well block. The system is delivered with one block type (a 96-well block in this case); other block types may be purchased separately. For a system that also supports TaqMan® array cards, as well as automation, please see our QuantStudio™ 7 Flex system. The QuantStudio™ 6 Flex system can also be upgraded to a QuantStudio™ 7 Flex system at a later date, as your needs change. For the ultimate in productivity, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

Upgrade Capabilities When You Need Them

The QuantStudio™ 6 Flex system can be seamlessly upgraded to a QuantStudio™ 7 Flex system by a field service engineer to give you additional automation, throughput, and multiplexing capabilities.

Results You Can Trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex® system, which features 5 coupled excitation and emission filter channels.

Sensitive Data Analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

RapidFinder™ Analysis Software v1.0 (Thermo Scientific™)

Optimize your pathogen detection workflow and enhance your downstream decision making process with this graphical, easy-to-use software.

QuantStudio™ 5 Real-Time PCR System, 96-well, 0.1 mL, desktop (Applied Biosystems™)

The Applied Biosystems® QuantStudio® 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio® 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 6 decoupled channels and white LED) and featuring six independent Veriflex® temperature zones, the QuantStudio® 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio® 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

Quantstudio 3/5 Firmware and Design & Analysis Software >

Features of the QuantStudio® 5 system with 96-well block include:

• Proven OptiFlex® technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Easy to use
The QuantStudio® 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio® Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio® Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio® 5 system offers users greater control of experiment data

Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex® technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

QuantStudio™ 12K Flex Real-Time PCR System, Array Card block, laptop (Applied Biosystems™)

The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems™ real-time technology in a single instrument. The QuantStudio 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray™ plates, TaqMan™ array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it's now or in the future, for maximum flexibility and consistency in your research.

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

The QuantStudio 12K Flex System's intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

Flexibility & Scalability
Includes five interchangeable blocks: The QuantStudio 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan array cards, and OpenArray plates, for effortless scaling from 1 to 12,000 data points in a single run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan array cards and OpenArray plates.

• Compatible with a full range of TaqMan assays: The QuantStudio 12K Flex System is optimized to enable clear, clean data for all TaqMan assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.

• Provides a seamless switch from qPCR to digital PCR: Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio Digital PCR kits and DigitalSuite™ Software with the QuantStudio OpenArray block.

• Delivers peace of mind: Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.

Maximum Throughput
Enhanced OptiFlex™ System: Building upon Applied Biosystems technology, the Enhanced OptiFlex System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single run.

• Unprecedented coverage and sample throughput: The OpenArray format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.

• Simple OpenArray workflow: QuantStudio Open Array plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.

• Integrated analysis and quality systems: New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.

QuantStudio 12K Flex Software
• Intuitive interface and innovative design for novice and experienced users
• Convenient walk-away operation
• Improved data analysis across block types
• Fully compatible with high-throughput environments

Expandable Software Architecture
Optional add-ons are available to upgrade the existing software:
21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.

• High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.

• DigitalSuite Software v1.0: For OpenArray block users needing sensitivity for rare targets and precision to measure small differences in target abundance.

• ExpressionSuite Software v1.0: Stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray plates.

QuantStudio™ 7 Flex Real-Time PCR System, 384-well, laptop (Applied Biosystems™)

The Applied Biosystems® QuantStudio™ 7 Flex Real-Time PCR System advances your research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, intuitive software, automation capabilities, and touch-screen interface, the QuantStudio™ 7 Flex system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation.

Features of the QuantStudio™ 7 Flex Real-Time PCR System include:

• Increased application versatility through the accomodation of 4 different block types
• Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
• The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
• Easy-to-use software, responsive touch-screen, automation capabilities, and effortless block exchange without the need for any tools

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

>> Download a 90-day trial of the QuantStudio™ 7 software

The QuantStudio™ 7 Flex system accommodates the interchange of a 96-well, 96-well Fast, 384-well, or TaqMan® array card block. The system is delivered with one block type (a 384-well block in this case); other block types may be purchased separately. In addition, a Twister® Robot Automation Accessory is available for this system to support high throughput workflows. For a more value-priced system that does not support TaqMan® array cards or automation (but that can be upgraded to do so at a later date), please see our QuantStudio™ 6 Flex system. And for the ultimate in flexibility, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

Accomplish More, Faster
Run hundreds of real-time PCR reactions effortlessly using TaqMan® Array 384-well microfluidic cards. And when integrated with the Twister® Robot Automation Accessory (available separately), the QuantStudio™ 7 Flex system maximizes your throughput capabilities.

High Application Versatility
Simple workflows, reliable assays and reagents, multiple formats, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, High Resolution Melt (HRM), and Protein Thermal Shift™ experiments.

Results You Can Trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex™ system, which features 6 decoupled excitation and emission filter channels with 21 filter combinations for maximum multiplexing and chemistry flexibility.

Sensitive Data Analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

QuantStudio™ 12K Flex Real-Time PCR System, 96-well block, laptop (Applied Biosystems™)

The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems™ real-time technology in a single instrument. The QuantStudio 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray™ plates, TaqMan™ array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it's now or in the future, for maximum flexibility and consistency in your research.

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

The QuantStudio 12K Flex System's intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

Flexibility & Scalability
Includes five interchangeable blocks: The QuantStudio 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan array cards, and OpenArray plates, for effortless scaling from 1 to 12,000 data points in a single run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan array cards and OpenArray plates.

• Compatible with a full range of TaqMan assays: The QuantStudio 12K Flex System is optimized to enable clear, clean data for all TaqMan assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.

• Provides a seamless switch from qPCR to digital PCR: Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio Digital PCR kits and DigitalSuite™ Software with the QuantStudio OpenArray block.

• Delivers peace of mind: Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.

Maximum Throughput
Enhanced OptiFlex™ System: Building upon Applied Biosystems technology, the Enhanced OptiFlex System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single run.

• Unprecedented coverage and sample throughput: The OpenArray format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.

• Simple OpenArray workflow: QuantStudio Open Array plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.

• Integrated analysis and quality systems: New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.

QuantStudio 12K Flex Software
• Intuitive interface and innovative design for novice and experienced users
• Convenient walk-away operation
• Improved data analysis across block types
• Fully compatible with high-throughput environments

Expandable Software Architecture
Optional add-ons are available to upgrade the existing software:
21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.

• High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.

• DigitalSuite Software v1.0: For OpenArray block users needing sensitivity for rare targets and precision to measure small differences in target abundance.

• ExpressionSuite Software v1.0: Stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray plates.

Pharmaceutical Analytics QuantStudio™ 3 Real-Time PCR System, 96-well, 0.2 mL, desktop (Applied Biosystems™)

The Applied Biosystems™ QuantStudio™ 3 Real-Time PCR System for Pharmaceutical Analytics is designed for users who need an affordable, easy-to-use real-time PCR system that doesn't compromise performance and quality. The QuantStudio 3 pharmaceutical analytics system is used together with specialized sample preparation and quantitation kits for host cell protein, host cell DNA, and Protein A impurity testing of biological products produced in cell culture.

The simplified Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio 3 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 4 coupled channels and white LED) and featuring three independent Veriflex® temperature zones, the QuantStudio 3 system enables improved data accuracy and sensitivity for a broad range of genomic applications.

Features of the QuantStudio 3 system include:

• Simplified instrument software with interactive touchscreen for greater ease of use
• Proven OptiFlex technology (4 coupled channels with white LED) for trusted results
• Secure cloud-based software option provides increased accessibility and storage options
• User account management, locked workflow, and Pause features for greater control of experiments
• 96-well 0.2 mL format or 96-well 0.1 mL format

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Downloads
>> QuantStudio 3/5 Design & Analysis Software | Release Notes | Example Data & Templates
>> QuantStudio 3/5 Firmware | Release Notes

Easy to use
The QuantStudio 3 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology, which features 4 coupled excitation and emission filter channels with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

QuantStudio™ 6 Pro Real-Time PCR System, 96-well, 0.2 mL (Applied Biosystems™)

The Applied Biosystems QuantStudio 6 Pro Real-Time PCR System adds innovative smart features to the real-time PCR (qPCR) workflow, all in a compact footprint. With facial authentication, voice commands, Smart Help, multiplex capability, motorized interchangeable blocks, simplified workflow, intuitive software, and large capacitive touch-screen interface, the QuantStudio 6 Pro system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Designed for ultimate ease-of-use, the system offers high quality, excellent reliability, and an optimal user experience for researchers who want to work smarter in the lab.

Features of the QuantStudio 6 Pro Real-Time PCR System include:
• Easy and convenient hands-free operation with facial authentication and voice commands
• Reduced errors and manual entries with smart qPCR workflow and TaqMan Array Plates with RFID
• Space savings with small footprint and true standalone operation; set-up, start, monitor, and analyze directly on the touchscreen
• Peace of mind with Smart Help; contact Technical Support directly from the touchscreen
• Reduced downtime with Smart Remote Support; show issues to Technical Support for speedy resolution
• Application versatility with easily changed blocks and three different block types
• Access, analyze, and share data anytime, anywhere with our Connect cloud-based platform for real-time information access, secure data storage, and online results sharing
• Remotely monitor experiments using the Instrument Connect monitoring app
• Design and Analysis software compatible with PC and Mac operating systems
• Compatible with over 8 million TaqMan Assays for gene expression, genetic variation, gene regulation, or protein expression experiments

Easy and convenient hands-free operation
The QuantStudio 6 Pro system was designed for a simple and powerful qPCR experience. Hands-free operation with facial authentication and voice commands enables users to quickly and easily access their personal profiles and settings without even removing their gloves.

Smart qPCR for ultimate ease of use
With embedded RFID readers, the QuantStudio 6 Pro system has the capability to run TaqMan Array Plates with RFID tags. These RFID tags simplify data tracking (with embedded lot number, expiration date, etc.) and reduce the need for manual data entry with preloaded assay layout, dye information, and thermal protocol. Automating this data transfer allows users to simply load the plate and start the run—no data entry needed.

Learn more about the TaqMan Array Plates with RFID ›

Peace of mind with Smart service
Minimize downtime with premium service offerings for the QuantStudio 6 Pro system. Smart Help allows users to contact Technical Support directly from the touchscreen, without the need to download files or spend time on the phone. Experienced technicians will analyze the request and respond according to the user’s preference (phone or email) and resolve the issue as quickly as possible.

Smart Remote Support is an immersive collaboration tool that allows customers to quickly and efficiently show issues to Technical Support for resolution, helping reduce instrument downtime compared to on-site repair and helping minimize the need for on-site visits.

Connect
The Connect platform provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the platform are web browser-based software tools that are flexible, fast, and easy to use. These software tools support a variety of analyses including relative quantitation, standard curve, high resolution melt (HRM), genotyping, and presence/absence. They are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

Format flexibility
The QuantStudio 6 Pro system accommodates the interchange of 96-well (0.2 mL), 96-well (0.1 mL), and 384-well blocks. The system is delivered with one block type; other block types may be purchased separately. For a system that also supports TaqMan array cards (TAC), as well as automation, please see our QuantStudio 7 Pro Real-Time PCR System. The QuantStudio 6 Pro system can also be upgraded to a QuantStudio 7 Pro system at a later date, as your needs change. And for the ultimate in flexibility, consider the QuantStudio 12K Flex Real-Time PCR System, which also accommodates TaqMan OpenArray plates.

Upgrade capabilities when you need them
The QuantStudio 6 Pro system can be seamlessly upgraded to a QuantStudio 7 Pro system by a field service engineer to give you additional automation, throughput, and multiplexing capabilities.

High application versatility
Simple workflows, reliable assays and reagents, multiple formats, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, and gene regulation.

Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex system, which features five coupled excitation and emission filter channels.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

StepOnePlus™ Real-Time PCR System with Tower (Applied Biosystems™)

The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications. We supply a Dell™ Tower computer with the StepOnePlus™ Real-Time PCR System.

Features of the StepOnePlus™ Real-Time PCR System include:

• Advanced software and instrumentation for performing a wide array of genomic assays
• Sensitive 4-color optical LED recording system
• Intuitive and robust software perfect for both first-time and advanced users
• Simple and flexible instrument set-up and usage

New StepOne™ Software
The StepOne™ Software included with the StepOnePlus™ Real-Time PCR System runs on the Windows® XP operating system and provides instrument control, data collection, and data analysis capabilities (analysis software is also compatible with Windows® 7). This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.

Rapid and Simple Assay Set-Up
This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOnePlus™ Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.

Sensitive LED-Based 4 Color Fluorescence Reading
The StepOnePlus™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, NED™/TAMRA™, and ROX™ dyes. This cost-effective, 4-color, 96-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.

Compatible with Many Genomic Analysis Techniques
Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus™ Real-Time PCR System and StepOne™ Software.

The StepOnePlus™ System supports many Real-Time PCR applications, including the following:

• SNP Genotyping
• Gene Expression Analysis
• MicroRNA Expression
• Protein Expression
• Translocation Analysis
• Gene Detection
• Viral Load Analysis

The included StepOne™ Software supports a variety of analysis methods, including the following:

• Standard Curve (absolute quantitation)
• Relative Standard Curve
• Comparative Ct (ΔΔCt) (relative quantitation)
• Genotyping and Presence/Absence
• Melt Curve Analysis (as a standalone application)
High Resolution Melting (as a standalone application)

Versatile Instrument Configuration Options
The ultra-compact footprint of a StepOnePlus™ System can be installed in multiple distinct configurations, providing unmatched flexibility and convenience that can allow a fit in any laboratory (Fig 2). The StepOnePlus™ Real-Time PCR System can be used via a PC, with a PC connected to a Local Area Network (LAN), or as a stand-alone instrument (PC-free). Each StepOnePlus™ Real-Time PCR System is factory-calibrated for optical and thermal accuracy, so simply remarkable Real-Time PCR results can be obtained right out of the box.

Energy Efficiency and Space Saving
The StepOnePlus™ Real-Time PCR System draws 38% less energy to process one sample plate (when the instrument is in a heated state), compared to the Bio-Rad CFX96™ Real-Time PCR Detection System. The StepOnePlus™ Real-Time PCR System also has advanced temperature control through use of VeriFlex™ Blocks technology and has a footprint nearly 15% smaller than Bio-Rad CFX96™, which in today’s crowded laboratories helps you use your laboratory space even more efficiently. You also leave a smaller environmental footprint on the Earth.

Real-Time Data Monitoring, Dissemination, and Storage
The system measures amplification as it occurs, allowing you to monitor the progress of your experiment cycle by cycle, either on the machine or remotely. Your data is stored on the instrument itself, and can be viewed and stored automatically via remote access, or transferred via email or a USB flash drive. Data can also be conveniently exported as PowerPoint®, Excel®, and graphical image files.

Expanded Gene Expression Capabilities
The advanced software provided with the StepOnePlus™ Real-Time PCR System now includes the powerful Gene Expression Study Package. This software package allows for greater flexibility and accuracy in your gene expression assays through:

• Analysis of an unlimited number of plates in one study
• Sorting of data by biological or technical replicate group
• Use of multiple endogenous controls
• Assay efficiency correction

Advanced High Resolution Melting Capability
Now with High Resolution Melt Software v3.0, you can perform post-PCR sequence variation analysis with the StepOnePlus™ Real-Time PCR System. This separately purchased software simplifies set-up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel. HRM Software v3.0 also has an improved clustering algorithm for increased sensitivity and accuracy, and the ability to conduct separate analyses of multiple assays run on a single plate.

QuantStudio™ 5 Real-Time PCR System, 96-well, 0.2 mL (Applied Biosystems™)

The Applied Biosystems® QuantStudio® 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio® 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 6 decoupled channels and white LED) and featuring six independent Veriflex® temperature zones, the QuantStudio® 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio® 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

Quantstudio 3/5 Firmware and Design & Analysis Software >

Features of the QuantStudio® 5 system with 96-well block include:

• Proven OptiFlex® technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Easy to use
The QuantStudio® 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio® Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio® Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio® 5 system offers users greater control of experiment data

Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex® technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.