Shop All Sequencing & Genetic Analyzer Instruments

3130 Genetic Analyzer (factory refurbished) (Applied Biosystems™)

With the Applied Biosystems 3130 Genetic Analyzer, you get the sophisticated automation and superior performance of the 3130 system, at acquisition and operating costs tailored to a growing research lab. This instrument is refurbished by the original manufacturer to meet both manufacturing and product specifications. This system includes the refurbished instrument, new Windows computer, Data Collection software, choice of one analysis software package, installation by an Applied Biosystems “5-Step Certified" Field Service Engineer, chemistry kit, training, new parts and a one year warranty. Availability may be limited.

• Run a wide variety of sequencing and fragment analysis applications including microsatellite analysis, AFLP, LOH, SNP validation, and SNP screening.
• Reduce maintenance time by eliminating manual syringe washing and filling with automated polymer delivery.
• Increase your data quality for sequencing and fragment analysis applications – longer read length, and higher resolution with shorter run times.
• Increase laboratory productivity and turnaround time by processing 96- or 384-well plates with a four-capillary array.

Perform a Wide Range of Applications
The Applied Biosystems 3130 Genetic Analyzer is more than just a DNA sequencer. You can run a wide variety of sequencing and fragment analysis applications including microsatellite analysis, AFLP, LOH, SNP validation, and SNP screening – as well as de novo sequencing and resequencing (mutational profiling). The full range of applications can be run on a single polymer and capillary array meaning you can run mixed applications on one plate. The software even includes tools to assist with regulatory and compliance requirements (In the United States, this assists with FDA 21CFR part 11).

Easy to Use
Reduce maintenance time by eliminating manual syringe washing and filling with the new automated polymer delivery system. No more handling of polymer syringes for set up and maintenance. Samples are automatically injected into the four-capillary arrays, and – unlike slab-gel systems – only minimal amounts of DNA are required for accurate analysis. Seamlessly switch between sequencing and fragment analysis runs, even in the same plate with the expanded one-polymer, one-array functionality for both sequencing and fragment analysis applications.

Superior Data Quality
Simply set up your sample and then sit back and watch as the analyzer's ultra-rapid sequencing gives you high-quality data (with Length of Read longer than 500 bp) in less than 35 minutes. Achieve accurate read lengths of 1,000 base pairs (bp) or longer in a single sequencing reaction using the 80 cm capillary array and the 3130 POP-7™ polymer.

Ideal for Growing Labs
The flexible, four-capillary 3130 system gives you all the advanced automation and superior performance of Applied Biosystems 3130xl platform, at acquisition and operating costs tailored to a growing research lab. And as your throughput needs increase, you can easily upgrade the system to 16 capillaries. It is the perfect way to get the capacity and savings you need today – without limiting your growth options. Researchers who own an ABI PRISM® 3100-Avant or 3100 Genetic Analyzer can upgrade to the new Applied Biosystems 3130 or 3130xl Genetic Analyzer.

3130 and 3130xl instruments are CE marked and compliant with the specifications and requirements as set in the EMC directive 89⁄336⁄EEC and the Low Voltage directive 73⁄23⁄EEC.

For Research Use Only. Not for use in diagnostics procedures.

SeqStudio™ Genetic Analyzer System with SmartStart (Applied Biosystems™)

The Applied Biosystems SeqStudio Genetic Analyzer is a 4-capillary, fluorescence-based capillary electrophoresis system designed to provide users with an integrated experience that is easy, efficient, and versatile for research in genetic analysis. The all-in-one reagent cartridge allows easy operation for multiple users with the flexibility to perform both sequencing and fragment analysis in a single run. The simplified SeqStudio Plate Manger software is well-suited for first-time and experienced users. Be more connected to your data than ever with intuitive browser-based software and cloud-based connectivity options for remote monitoring, and data transfer and analysis.

The SeqStudio system gives you the same data quality, reliability, and support you have come to expect from the Applied Biosystems brand with a modernized experience. Same workflow, same trusted data. Now with one-click convenience.

Key features
• Simplified instrument software with interactive touch screen for greater ease of use
• Minimized hands-on setup time with integrated reagents cartridge (SeqStudio Cartridge)
• Application flexibility to run Sanger sequencing and fragment analysis on the same run
• Connectivity: Wi-Fi or wired Ethernet connection
• Web-enabled remote monitoring capability
• Consumables usage tracking through radio frequency identification (RFID)

This package includes:
• 4-capillary SeqStudio Genetic Analyzer
• SeqStudio Analysis Software (GeneMapper, Sequencing Analysis, Minor Variant Finder, Variant Reporter, and SeqScape)
• SeqStudio Plate Manager Software
• 1Day SmartStart Orientation
Note: SeqStudio Cartridge and other system consumables and reagents sold separately

Just click. That's it.
Load up your samples, click in the cartridge, and go—no need for technical experts for setup or maintenance, no calibration before each run, and no installation of capillaries, polymer, and anode buffer. The cartridge is stable for 4 months on the instrument.

Access your data anytime, anywhere*
This system features Thermo Fisher Cloud connectivity so you can set up your run, check its progress, and access your data from wherever you have Internet access, all without the need to stay in the lab.* Get the same data quality, service, and support you’ve come to expect from Applied Biosystems technology with a modernized experience at a price any lab can afford.
* Internet connection and Thermo Fisher Cloud account required.

Service, support, and training
We offer a range of service options, plans, and warranties to improve productivity, increase instrument uptime, and lower the total cost of ownership. SmartStart orientation helps get you started with your new instrument more quickly.

Software
SeqStudio Genetic Analyzer Instrument Software, v 1.1Software update fileRelease notesEULA
SeqStudio Genetic Analyzer Plate Manager Software, v1.1Windows installerMac installer
Sequence Analysis Software patch for SeqStudio supportInstallerRelease notes 
SeqScape Software patch for SeqStudio supportInstallerRelease notes 
Variant Reporter Software patch for SeqStudio supportInstallerRelease notes 
GeneMapper Client Software patch for SeqStudio supportInstallerRelease notes 
GeneMapper Full Software patch for SeqStudio supportInstallerRelease notes 
Minor Variant Finder Software patch for SeqStudio supportInstaller 


3500xL Genetic Analyzer for Fragment Analysis (Applied Biosystems™)

The 3500xL Genetic Analyzer is an 24-capillary sequencing instrument specifically designed to support the demanding performance needs of validated and process-controlled environments, while retaining the unsurpassed application versatility that life science researchers expect. The analyzer supports a wide variety of applications, including de novo sequencing and re-sequencing (mutational profiling), as well as microsatellite analysis, MLPA™, LOH, MLST, AFLP®, and SNP validation or screening. The majority of applications can be run on a single polymer and capillary array.

In addition to the 3500xL Genetic Analyzer, this fragment analysis package includes 3500 Series Data Collection Software, a Dell Workstation and monitor, reagent kits for system qualification, and secondary analysis software. 3500 Series Data Collection Software supports sequencing and fragment analysis, and integrates seamlessly with downstream software for secondary analysis of genetic data. GeneMapper™ secondary analysis software is included in this package.

Benefits and features of the 3500 Genetic Analyzer include:
Cost effective—lower running costs per sample and increased reagent life (14 days)
Easy setup—no special power outlets or external exhaust needed
Increased multiplexing—6-dye capability to interrogate more SNPs or markers
Easy-to-use software—integrated data collection and primary analysis software for real-time assessment of data quality
Consumables tracking—radio frequency identification (RFID) technology used for residual volume and expiry date tracking
Improved data uniformity—instrument-to-instrument, run-to-run, and capillary-to-capillary
More stable and precise fragment sizing due to improved oven temperature control
Smaller footprint versus 3130/3130xl instruments

Accurate and reliable data quality
The 3500 series systems significantly improve signal uniformity from instrument to instrument, run to run, and capillary to capillary. The combination of intelligent hardware and powerful algorithm and reagent combinations reduce the range of signal peak heights obtained across multiple 3500 series instruments.

Improved sizing precision
The thermal sub-system design provides enhanced performance during demanding fragment analysis applications, enabling improved sizing precision versus previous generations of capillary electrophoresis platforms.

Ready-to-use consumables with RFID tags
The 3500 series utilizes ready-to-use consumables with pre-packaged polymer pouches, cathode and anode buffer containers, and easy-to-install capillary arrays. Each of these consumables includes an integrated RFID tag that enables viewing, tracking, and reporting of critical information, including usage, lot number, part number, expiry date, and on-instrument lifetime, all within the 3500 Series Data Collection Software.

Easy-to-use data collection software
3500 Series Data Collection software features user-friendly navigation with an intuitive dashboard design, highly visible buttons for common operations, easy-to-read graphical displays to monitor the state of consumables, and a handy maintenance scheduling calendar. Additional features include simplified plate set-up and built-in primary analysis with quality control, so you can make decisions about the quality of data as it is produced on the instrument without the need to transfer output files into secondary analysis software packages.

Ion S5™ System (Ion Torrent™)

The Ion S5™ next-generation sequencing system enables a simple targeted sequencing workflow for your lab at an affordable price, without compromising on performance or reliability. The Ion S5 System leverages the speed of semiconductor sequencing with impressive on-board computing power, to enable the production of high quality sequencing data in as little as 2.5 hours and enable you to go from DNA library to data in as little as 24 hours with only 45 minutes of total hands-on time on the Ion Chef™ System and the Ion S5 System. The Ion S5 System is simple to use with cartridge-based reagents and offers superior scalability and flexibility to support a broad range of high throughput sequencing applications, from microbial genomes and gene panels to exomes and transcriptomes, using our 5 Series chips.

Speed
Some research decisions can’t wait. A few hours could make all the difference in your quest for the right answer.With other light-based sequencers you could wait almost 60 hours for results. With the Ion S5 System you get results in as little as 24 hours (with a 2.5-4 hour sequencer run time and overnight Ion Chef System run).

Simplicity
With single-day installation, a straightforward user interface, and cartridge-loaded reagents, the Ion S5 System is easy to use. Beyond this, the system is part of a workflow that allows you to go from DNA to data with only 45 minutes of total hands-on time on the Ion Chef System and the Ion S5 System, significantly less than other targeted sequencing solutions that require as much as 2.5 hours of hands-on time. Combined with Ion AmpliSeq™ technology for target selection, the Ion Chef™ System for automated library and template preparation, and Ion Reporter™ Software for automated variant annotation, targeted sequencing becomes simpler than ever.

Small sample input
Low input DNA for challenging samples types, such as formalin-fixed paraffin-embedded (FFPE) tissue, retrospective samples from fine needle aspirates, and cell-free DNA extracted from blood (cfDNA) can be difficult to sequence on competititor next-generation sequencers. However, with Ion AmpliSeq technology and the Ion S5 Sequencer, you can use as little as 10 ng input DNA or RNA.

Scalability
Why buy multiple systems for your lab when you can run all of your targeted sequencing or microbial applications on a single next-generation sequencer? The Ion S5 System has a modular build, so your lab has the flexibility to do multiple targeted sequencing applications on a single system.

Simple analysis and storage solutions
Whether you are new to next-generation sequencing or an expert user, Torrent Suite™ Software and Ion Reporter™ Software make it easy for you to get started with next-generation sequencing. Plan, monitor, track, and analyze your runs using Torrent Suite Software (pre-installed on the Torrent Server). Integrate, annotate, and interpret variants using Ion Reporter Software (Cloud or local options available based on your needs).

Services and support
With more than 2,000 field personnel, Thermo Fisher Scientific has the most extensive network of dedicated Field Application Specialists and Service Engineers to work with you, so you can be confident that we will be there when and where you need us most.

Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data

Ion GeneStudio™ S5 Plus System for Agrigenomics (Ion Torrent™)

The Ion GeneStudio S5 Plus System is a semiconductor-based next-generation sequencing (NGS) system that enables simple targeted sequencing workflows. From genes to exomes, the Ion GeneStudio S5 Plus System is designed to enable flexibility to help labs manage small and large projects across multiple applications. With cartridge-based reagents, the system is simple to use and offers scalability and flexibility. It uses Ion 5 Series chips to support a broad range of high-throughput sequencing for clinical research and research applications from microbial genomes and gene panels to exomes and transcriptomes.

Key features:
• Select the chip (Ion 510–550 chip) that fits your application and throughput needs to achieve from 2–130 million reads per run
• Reduce setup time and complexity with cartridge-based plug-and-play reagents
• Complete run and analysis in hours and move forward with your experiments

The Ion GeneStudio S5 Series
The Ion GeneStudio S5 Series sequencers share the same foundational technology and fluidic architecture across all three instruments: the Ion GeneStudio S5 System, this Ion GeneStudio S5 Plus System, and Ion GeneStudio S5 Prime System. The primary difference across the three instruments is on-sequencer analysis time.

Speed
The Ion GeneStudio S5 Series leverages the speed of semiconductor sequencing to enable the production of high quality sequencing data in a few hours and enables you to go from DNA library to data in as little as 24 hours with only 45 minutes of total hands-on time when paired with the Ion Chef System.

Simplicity
With single-day installation, a straightforward user interface, and cartridge-based reagents, the Ion GeneStudio S5 Series sequencers are easy to use. Combined with Ion AmpliSeq technology for target selection, the Ion Chef System for automated library and template preparation, and Ion Reporter Software for automated variant annotation, targeted sequencing is simpler than ever.

Small sample input
Low input DNA from challenging samples types such as formalin-fixed paraffin-embedded (FFPE) tissue, retrospective samples from fine needle aspirates, and cell-free DNA extracted from blood (cfDNA) can be difficult to sequence on competitor next-generation sequencers. However, with Ion AmpliSeq technology and the Ion GeneStudio S5 Series, you can use as little as 1 ng input DNA or RNA.

Scalability
Why buy multiple systems for your lab when you can run all of your targeted sequencing or microbial applications on a single next-generation sequencer? The Ion 5 Series chips that run on the Ion GeneStudio S5 Series provide several throughput options, so your lab has the flexibility to scale from small to large projects, enabling multiple targeted sequencing applications on a single system.

Simple analysis and storage solutions
Torrent Suite Software and Ion Reporter Software make NGS easy for beginners and experts alike. Plan, monitor, track, and analyze your runs using Torrent Suite Software (pre-installed on the Torrent Server). Integrate, annotate, and interpret variants using Ion Reporter Software (Thermo Fisher Cloud or local options available based on your needs).

Services and support
With more than 2,000 field personnel, Thermo Fisher Scientific has the most extensive network of dedicated Field Application Specialists and Service Engineers to work with you, so you can be confident that we will be there when and where you need us most.

Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data

ABI PRISM 3100 Genetic Analyzer to 3130xl System Upgrade with computer (Applied Biosystems™)

This is a system upgrade, from the 3100 Genetic Analyzer to the Applied Biosystems® 3130xl System.

The 3130xl Genetic Analyzer is the latest generation of 16-capillary electrophoresis instruments for the medium throughput laboratories. The system offers industry-leading performance, plus sophisticated automation capabilities allowing researchers to save time, reduce costs and increase productivity.

Important Note: 3100 System Sequencers will be retired at the end of 2011. Take advantage of our limited time promotion to either TRADE-IN your 3100 for a brand new 3500 or UPGRADE to a 3130. Please contact your local Sales Representative to learn more.

For Research Use Only. Not for use in diagnostics procedures.

3500xL Genetic Analyzer for Protein Quality Analysis, with software (Applied Biosystems™)

The 3500xL Genetic Analyzer for Protein Quality Analysis is a capillary electrophoresis instrument with a 24-capillary array specifically designed to support the demanding performance needs of biopharmaceutical screening and quality control environments, while retaining the unsurpassed application versatility appreciated by scientists working in analytical and process development environments. The instrument enables parallel analysis of 24 samples without compromising on the run time per sample, thereby enabling high throughput analysis with high quality data. This system includes a 24-capillary instrument, GlycanAssure™ Acquisition and Analysis Software, a Dell Workstation and monitor, as well as reagent kits for system qualification.

Features include:
• A 24-capillary system for high throughput analysis
• Single-line, 505 nm, solid-state, long-life laser that utilizes a standard power supply and requires no heat-removal
• Powerful, integrated data acquisition and analysis software that provides real-time assessment of data
• Radio frequency identification (RFID) technology that tracks key consumables data and records administrative information
• Advanced multiplexing capabilities for glycan analysis with up to six unique dyes
• Unrivaled application flexibility—one array and one polymer are used for most applications
• Simple setup, operation, and maintenance

Request more information >

Accurate, precise, and reliable data quality
The 3500 Series systems significantly improve signal uniformity from instrument-to-instrument, run-to-run, and capillary array-to-capillary array. The system provides accurate, reproducible, and precise relative quantitation of glycan levels.

Ready-to-use consumables with RFID tags
The 3500 Series systems introduce ready-to-use consumables with pre-packaged polymer pouches, cathode and anode buffer containers, and easy-to-install capillary arrays. Each of these consumables is integrated with RFID tags that enable viewing, tracking, and reporting of critical information about reagents and consumables, including usage, lot number, part number, expiry date, and on-instrument lifetime within the GlycanAssure data acquisition software.

Easy-to-use data acquisition and data analysis software
GlycanAssure data acquisition software breaks new ground in user-friendly navigation with an intuitive dashboard design, highly visible buttons for common operations, easy-to-read graphical displays to monitor the state of consumables, simplified plate setup, and a handy maintenance scheduling calendar functionality. GlycanAssure data analysis software allows quick viewing of analyzed and un-analyzed samples and quick comparison of different analysis methods. It includes built-in primary analysis with quality control, so you can make decisions about the quality of data as it is produced without the need to transfer output files to other software.

Additional products
The following products are required for use with this system:
A31278: Anode Buffer Container (ABC) for 3500 Series for Protein Quality Analysis
A31279: Cathode Buffer Container (CBC) for 3500 Series for Protein Quality Analysis
A30936: POP-7™ for Protein Quality Analysis (384 samples)
A31122: POP-7™ for Protein Quality Analysis (960 samples)



3730xl DNA Analyzer (factory refurbished) (Applied Biosystems™)

The 96-capillary 3730xl DNA Analyzer is the Gold Standard for high throughput genetic analysis. Use this for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample. This instrument is refurbished by the original manufacturer to meet both manufacturing and product specifications. This system includes the refurbished instrument, new Windows computer, Data Collection software, choice of one analysis software package, installation by an Applied Biosystems “5-Step Certified" Field Service Engineer, chemistry kit, training, new parts and a one year warranty. Availability may be limited.

• Higher optical sensitivity and advanced polymers enable you to obtain higher-quality sequencing data for less.
• Multiple automation features decrease costly human errors.
• Optimized polymers increase your productivity without compromising your results.
• Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation.

Large-Scale DNA Analysis on a Small-Scale Budget
By combining advances in automation with innovative optics and proprietary reagents to increase throughput, yield high-quality data, and minimize reagent consumption, the 3730xl Analyzer provides your laboratory and production facility with faster, better, cheaper analysis.

Streamline Your Workflow
The 3730xl DNA Analyzer system is engineered for highly reliable, unattended operation of up to 48 hours (applies to modules with run times longer than 30 minutes). To minimize the need for operator intervention and decrease the risk of human error, automation features include an integrated plate stacker, internal bar code reader, and onboard polymer delivery system.

Increase Productivity
The high signal-to-noise ratio ensures you get high-quality data – even when you use low-concentration samples and reagents. Labor-saving automation features minimize hands-on time and enable you to analyze more data more efficiently.

Get the Highest-Quality Sequencing and Genotyping Data
The enhanced optical design provides a higher signal-to-noise ratio and a more uniform signal profile across the array. This design, combined with our new, advanced polymer, enables the longest read lengths of any available system, and provides enhanced color balance for streamlined genotyping sample handling. In addition, exceptional sensitivity enables higher success rates across a wide range of sample template types and concentrations.

One Instrument, Multiple Applications
With the 3730xl DNA Analyzer, you get the highest-quality data from a wide array of applications. In addition to being ideal for high-throughput sequencing, the analyzer's optimized application assays, instrument, and analysis software also provide a complete solution for genotyping and resequencing.

Integrated Data Analysis Tools Reduce Time-to-Results
The 3730xl DNA Analyzer software suite allows you to generate more meaningful data with less work. This system's labor-saving software suite includes:
• Data Collection (supplied with the instrument): Manages your instrument setup, controls instrument operations, allows real-time data visualization, and performs diagnostics.
• Sequencing Analysis Software: Designed to base-call; assign quality values; trim, display, edit and print DNA sequencing data using the KB basecaller.
• Seqscape®: Provides everything you need to perform resequencing applications such as VariantSEQr™ Resequencing System.
• GeneMapper®: Enables configurable, automated allele calling – a plus for high-throughput genotyping and includes tools for SNPlex™ data analysis

New features include:
• Auto-analysis with GeneMapper® and SeqScape®
• Flexibility to use any choice in dye set option
• Tools to assist with regulatory and compliance requirements (In the United States, this assists with FDA 21CFR part 11).
• Additional optimized run modules covering more applications
• Support for fragment analysis applications on the 96-capillary array

The instrument comes with a one year limited warranty on parts and labor.

For Research Use Only. Not for use in diagnostics procedures.

3500 Genetic Analyzer for Sequence Typing & Fragment Analysis (Applied Biosystems™)

This 8-capillary Genetic Analyzer is specifically configured with software for both fragment analysis and sequence typing applications.

We set a new standard in capillary electrophoresis with the 8-capillary 3500 Genetic Analyzer. These instruments are specifically designed to support the demanding performance needs of validated and process controlled environments, while retaining the unsurpassed application versatility that life science researchers expect from us.

• An 8 capillary system that can easily be upgraded to a 24-capillary system when you’re ready.
• New single-line 505 nm, solid-state long-life laser – utilizes a standard power supply; requires no heat-removal ducting.
• Powerful, integrated data collection and primary analysis software provides real-time assessment of data quality.
• Radio Frequency Identification (RFID) technology tracks key consumables data and records administrative information.
• Advanced multiplexing capabilities for DNA fragment analysis with up to six unique dyes.
• Unrivaled application flexibility – one array and one polymer are used for most applications.
• Simple setup, operation and maintenance – the easiest-to-run, easiest-to-own DNA sequencer to date.

Accurate Reliable Data Quality
The 3500 series systems significantly improves signal uniformity from instrument-to-instrument, run-to-run, and capillary-to-capillary. With a combination of intelligent hardware, powerful new algorithm and reagent combinations, these methods provide major reduction in the range of signal peak heights obtained across multiple 3500 series instruments (see figure 1).

Improved Sizing Precision
New thermal sub-system design gives enhanced performance in demanding fragment applications enabling improved sizing precision versus previous generations of capillary electrophoresis platforms (see figure 2).

Ready to Use Consumables With RFID Tags
The 3500 series introduces ready-to-use consumables with pre-packaged polymer pouches, cathode and anode buffer containers and easy-to-install capillary arrays. Each of these consumables is integrated with RFID tags that enable viewing, tracking and reporting of critical information about reagents and consumables including usage, lot number, part number, expiry date and on-instrument lifetime within the 3500 Series Data Collection Software (see figures 3 and 4).

Easy to Use New Data Collection Software
3500 Series Data Collection software breaks new ground in user-friendly navigation with an intuitive dashboard design, highly visible buttons for common operations, easy-to-read graphical displays to monitor the state of consumables and handy maintenance scheduling calendar functionality. New functionality includes simplified plate set-up, built-in primary analysis with quality control, so you can make decisions about the quality of data as it is produced on the instrument without the need to transfer output files into secondary analysis software packages (see figures 4 & 5).

For Research Use Only. Not for use in diagnostics procedures.

Ion PGM™ Dx System (Ion Torrent™)

The Ion PGM™ Dx System is a reliable and robust in vitro diagnostic (IVD) next-generation sequencing (NGS) platform that is designed to deliver the proven performance and simplicity of the Ion Torrent™ PGM system, while empowering clinical laboratories with the flexibility to develop molecular diagnostic assays to help meet their evolving needs.

The Ion PGM™ Dx System:
• Offers a complete, simple, and cost-effective IVD NGS instrument system solution
• Enables highly reproducible and accurate genetic variant analysis with as little as 10 ng of input DNA
• Provides rapid turnaround time from sample to variant analysis in <2 days
• Enables molecular diagnostic assay developers the flexibility to publish protocols in a locked workflow environment
• Helps increase laboratory efficiency with integrated software that supports sample and reagent tracking, auditing, and traceability compliance
• Supports role-based workflows to help ensure a higher level of results integrity

Proven technology and performance
The Ion PGM™ Dx System is built on proven Ion Torrent™ Post-Light™ Ion Semiconductor Sequencing technology that is well-established and adopted globally in life sciences and clinical research. With this system, the technology has been refined and designed specifically to help meet the increasing needs of the diagnostic laboratory environment. The system provides a comprehensive set of instruments, reagents & consumables, and integrated software developed under stringent design control with a complete design history file, and manufactured under GMP to help ensure performance and quality standards are met.

Results generated using the Ion PGM™ Dx System proved highly reproducible in a validation study across multiple sites, operators, and reagent kits. Overall system performance was excellent with an average SNV sensitivity of >99.5%. The system was validated using challenging germline variants, with library generation based on proven Ion AmpliSeq™ technology, enabling accurate and reliable genetic analysis even with input DNA amounts as low as 10 ng.

Flexibility & scalability
The Ion PGM™ Dx platform empowers clinical laboratories with the flexibility and scalability to reliably develop and implement their own molecular diagnostic assays using the Ion PGM™ Dx System and GMP manufactured reagent sets combined with their choice of targeted analyte specific reagents. Leveraging Ion Ampliseq™ ultrahigh-multiplex PCR targeted library generation technology, the system enables assay developers to define and customize their assay primer sets of any panel size, and to validate, lock, and publish protocols in a role-based workflow environment for implementation into routine use.

Simple & efficient workflow to help maximize throughput
The Ion PGM™ Dx System delivers the speed and simplicity of the Ion Torrent™ platform, combined with key workflow advantages designed specifically for regulated laboratory environments and in vitro diagnostic applications. A simplified instrument and assay workflow, as well as intuitive software, further automate the testing turnaround time, providing rapid sample-to-variant analysis results in as little as 2 days. Additionally, the validated workflow enables multiplexing of up to 16 samples within a single sequencing run, making it a cost-effective and flexible approach.

Integrated secure software helps ensure high integrity results
Ion PGM™ Dx Torrent Suite™ software is an integrated software system that manages and tracks the entire run process from sample definition to variant analysis, providing full data traceability and auditable run analysis—a necessary part of 21CFR Part 11 compliance. With a user-friendly intuitive software interface, multi-tier role-based workflows, and a suite of parameter controls, the software aids clinical laboratories in maintaining high performance standards and helps ensure a higher level of results integrity.

The unique capabilities and features of the Ion PGM™ Dx System designed specifically for regulated laboratory environments make it a suitable next-generation sequencing platform-of-choice for nearly every clinical diagnostic laboratory.

Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data

Intended use
The Ion PGM™ Dx Instrument System is composed of a sequencing instrument that measures the hydrogen ions that are generated during the incorporation of nucleotides in the DNA sequencing reaction and the ancillary instrumentation necessary for sample processing. This instrument system is used in conjunction with the instrument-specific Ion PGM™ Dx Library Kit, Ion OneTouch™ Dx Template Kit, Ion PGM™ Dx Sequencing Kit, and Ion 318™ Dx Chip Kit, and data analysis software. The Ion PGM™ Dx Instrument System is intended for targeted sequencing of human genomic DNA from peripheral whole-blood samples. The Ion PGM™ Dx Instrument System is not intended for whole genome or de novo sequencing.

HID Ion Chef™ Instrument (Ion Torrent™)

The HID Ion Chef System is the next generation of workflow simplification products for the HID Ion GeneStudio S5 Series sequencers. The HID Ion Chef System provides automated library preparation, template preparation, and chip loading for users at any experience level. In less than 15 minutes of up-front hands-on time and with the use of pre-packaged library preparation reagent kits, the HID Ion Chef System provides a convenient walk-away workflow resulting in equalized, pooled libraries ready for templating. The automated workflow supports Precision ID panels for both one- and two-pool designs. After library preparation, less than 15 minutes of hands-on time is required to set up the HID Ion Chef System to perform fully automated template preparation, producing one or two chips ready for sequencing. Automating your sample preparation workflows on the HID Ion Chef System results in significantly higher productivity.

The HID Ion Chef System:
• Simplifies the workflow
• Minimizes sources of user-introduced variability
• Helps save time and labor
• Supports sequencing preparation for the HID Ion GeneStudio S5 Systems

The HID Ion Chef System features include:
• Automated Precision ID library preparation, template preparation, and chip loading
• Cartridge-based reagent system
• Sample- and reagent-tracking capability
• Intuitive user interface
• On-board diagnostics

Simplify the forensic NGS workflow
The HID Ion Chef System simplifies the forensic next-generation sequencing (NGS) workflow by integrating several manual and instrument steps into a single process. The HID Ion Chef System workflow now incorporates all steps of library preparation (targeted PCR amplification with respective primer panel, partial digestion of primer sequences, adapter/barcode ligation, purification, library equalization and pooling), as well as all steps of template preparation and chip loading. The simple, automated workflow provides easy access for users of any experience level.

Minimize variability and load chips reproducibly
The HID Ion Chef System provides reproducible chip loading by automating multiple manual chip-loading steps. The sophisticated visual system inside the HID Ion Chef System is designed to help reduce user-introduced error and variability by providing barcode reading and positive identification of samples, chips, and reagents. The visual system performs the setup check before the run, as well as the reagents and consumables load and unload check, calibration, and diagnostics.

Save time and labor
The HID Ion Chef System enables significant time and labor savings by offering quick and easy setup for both library preparation and templating that typically requires less than 15 minutes of hands-on time for each step.

Support for all Ion Torrent sequencing workflows
The flexible HID Ion Chef System is designed to provide automated and consistent Precision ID library preparation and template preparation for sequencing on the HID Ion GeneStudio S5 systems using any of the corresponding chips or template preparation and sequencing chemistries.

Services and support
With more than 2,000 field personnel, Thermo Fisher Scientific has the most extensive network of dedicated Field Application Specialists and Service Engineers to work with you, so you can be confident that we will be there when and where you need us most.

Service and support plans—comprehensive plans to maximize instrument performance and help ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data

3500 Genetic Analyzer for Protein Quality Analysis, with software (Applied Biosystems™)

The 3500 Genetic Analyzer for Protein Quality Analysis is a capillary electrophoresis instrument with an 8-capillary array specifically designed to support the demanding performance needs of biopharmaceutical screening and quality control environments, while retaining the unsurpassed application versatility appreciated by scientists working in analytical and process development environments. The instrument enables parallel analysis of 8 samples without compromising on the run time per sample, thereby enabling high throughput analysis with high quality data. This system includes an 8-capillary instrument, GlycanAssure™ Acquisition and Analysis Software, a Dell Workstation and monitor, as well as reagent kits for system qualification.

Features include:
• An 8-capillary system that can easily be upgraded to a 24-capillary system when you need higher throughput
• Single-line, 505 nm, solid-state, long-life laser that utilizes a standard power supply and requires no heat removal
• Powerful, integrated data acquisition and analysis software that provides real-time assessment of data
• Radio frequency identification (RFID) technology that tracks key consumables data and records administrative information
• Advanced multiplexing capabilities for glycan analysis with up to six unique dyes
• Unrivaled application flexibility—one array and one polymer are used for most applications
• Simple setup, operation, and maintenance

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Accurate, precise, and reliable data quality
The 3500 Series systems significantly improve signal uniformity from instrument-to-instrument, run-to-run, and capillary array-to-capillary array. The systems provide accurate, reproducible, and precise relative quantitation of glycan levels.

Ready-to-use consumables with RFID tags
The 3500 Series systems introduce ready-to-use consumables with pre-packaged polymer pouches, cathode and anode buffer containers, and easy-to-install capillary arrays. Each of these consumables is integrated with RFID tags that enable viewing, tracking, and reporting of critical information about reagents and consumables, including usage, lot number, part number, expiry date, and on-instrument lifetime within the GlycanAssure data acquisition software.

Easy-to-Use data acquisition and data analysis software
GlycanAssure data acquisition software breaks new ground in user-friendly navigation with an intuitive dashboard design, highly visible buttons for common operations, easy-to-read graphical displays to monitor the state of consumables, simplified plate setup, and a handy maintenance scheduling calendar functionality. GlycanAssure data analysis software allows quick viewing of analyzed and un-analyzed samples and quick comparison of different analysis methods. It includes built-in primary analysis with quality control, so you can make decisions about the quality of data as it is produced without the need to transfer output files to other software.

Additional products
The following products are required for use with this system:
A31278: Anode Buffer Container (ABC) for 3500 Series for Protein Quality Analysis
A31279: Cathode Buffer Container (CBC) for 3500 Series for Protein Quality Analysis
A30936: POP-7™ for Protein Quality Analysis (384 samples)
A31122: POP-7™ for Protein Quality Analysis (960 samples)



3730xl DNA Analyzer (Applied Biosystems™)


See the latest version of the 3730xl DNA Analyzer ›

The 96-capillary 3730xl DNA Analyzer is the Gold Standard for high throughput genetic analysis. Use this for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample.
• Higher optical sensitivity and advanced polymers enable you to obtain higher-quality sequencing data for less.
• Multiple automation features decrease costly human errors.
• Optimized polymers increase your productivity without compromising your results.
• Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation.

Large-Scale DNA Analysis on a Small-Scale Budget
By combining advances in automation with innovative optics and proprietary reagents to increase throughput, yield high-quality data, and minimize reagent consumption, the 3730xl Analyzer provides your laboratory and production facility with faster, better, cheaper analysis.

Streamline Your Workflow
The 3730xl DNA Analyzer system is engineered for highly reliable, unattended operation of up to 48 hours (applies to modules with run times longer than 30 minutes). To minimize the need for operator intervention and decrease the risk of human error, automation features include an integrated plate stacker, internal bar code reader, and onboard polymer delivery system.

Increase Productivity
The high signal-to-noise ratio ensures you get high-quality data – even when you use low-concentration samples and reagents. Labor-saving automation features minimize hands-on time and enable you to analyze more data more efficiently.

Get the Highest-Quality Sequencing and Genotyping Data
The enhanced optical design provides a higher signal-to-noise ratio and a more uniform signal profile across the array. This design, combined with our new, advanced polymer, enables the longest read lengths of any available system, and provides enhanced color balance for streamlined genotyping sample handling. In addition, exceptional sensitivity enables higher success rates across a wide range of sample template types and concentrations.

One Instrument, Multiple Applications
With the 3730xl DNA Analyzer, you get the highest-quality data from a wide array of applications. In addition to being ideal for high-throughput sequencing, the analyzer's optimized application assays, instrument, and analysis software also provide a complete solution for genotyping and resequencing.

Integrated Data Analysis Tools Reduce Time-to-Results
The 3730xl DNA Analyzer software suite allows you to generate more meaningful data with less work. This system's labor-saving software suite includes:
• Data Collection (supplied with the instrument) – Manages your instrument setup, controls instrument operations, allows real-time data visualization, and performs diagnostics.
• Sequencing Analysis Software – Designed to base-call; assign quality values; trim, display, edit and print DNA sequencing data using the KB basecaller
• Seqscape® – Provides everything you need to perform resequencing applications such as VariantSEQr™ Resequencing System
• GeneMapper® – Enables configurable, automated allele calling; a plus for high-throughput genotyping and includes tools for SNPlex™ data analysis

New features include:
• Auto-analysis with GeneMapper® and SeqScape®
• Flexibility to use any choice in dye set option
• Tools to assist with regulatory and compliance requirements (In the United States, this assists with FDA 21CFR part 11).
• Additional optimized run modules covering more applications
• Support for fragment analysis applications on the 96-capillary array

The instrument comes with a one year limited warranty on parts and labor.

For Research Use Only. Not for use in diagnostics procedures.

Ion Personal Genome Machine™ (PGM™) System (Ion Torrent™)

The Ion Personal Genome Machine (PGM) System combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and making it accessible to your lab. This established system leverages the exponential improvements in the semiconductor industry (known as Moore’s Law) to provide scalability and flexibility for various applications. The Ion PGM System uses natural sequencing chemistry, which eliminates the need for expensive optics and reduces complex chemistries to measure natural DNA extension. The system leverages direct, real-time sequencing detection, providing sequencing results typically in 3–7 hours.

Affordable sequencing for small genomes and targeted panels
Other sequencing technologies rely on optical systems as bridges between chemical and digital sequence information. These systems typically entail significant additional costs; the elimination of the optical system by the Ion PGM System reduces these costs. With its small benchtop footprint, weight of about 65 lbs (30 kg), low-cost sequencing reagents, and simple touchscreen user interface, the Ion PGM System brings next-generation sequencing to your lab. Coupled with the Torrent Suite Software, the Ion PGM System performs preliminary reference genome alignment and outputs data in BAM format for easy downstream analysis with optional third party analysis tools. It can also provide access to a web portal for data review and sharing.

More information on the Ion Torrent Server >

Ion power: PostLight sequencing produces bits from bases
The sequencing technology underlying the Ion PGM System exploits a well-characterized biochemical process: when a polymerase incorporates a nucleotide into the growing strand of DNA, a hydrogen ion (H+) is released as a byproduct. This hydrogen ion carries a charge which the Ion PGM chip—essentially the world's smallest solid-state pH meter—can detect. As the sequencer floods the chip with one nucleotide after another, any nucleotide added to a DNA template will be detected as a voltage change, and the Ion PGM System will call the base. If a nucleotide is not a match for a particular template, no voltage change will be detected and no base will be called for that template.

Next-generation sequencing on your bench
A principal component of the Ion PGM System is the sequencing chip. This microprocessor chip incorporates an extremely dense array of >1 million micro-machined wells married to our proprietary ion sensor. Each well contains a different DNA template, allowing massively parallel sequencing. The chips can scale in density for specific applications from targeted panels to small genomes.

The right tool to get results easily, reliably, and quickly
Since it detects nucleotide incorporation without the use of light, the Ion PGM System uses the simplest sequencing chemistry possible: natural nucleotides. There is no need for expensive and error-prone modified bases, enzymatic cascades, chemiluminescence, or fluorescence. Direct detection also means the incorporation of each nucleotide is recorded in seconds. As a result, the Ion PGM System runs finish within hours rather than days.

Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data

For a more simplified next-generation sequencing workflow, see the Ion S5 System.

Learn more about the Ion PGM sequencing chips >

3730 DNA Analyzer (factory refurbished) (Applied Biosystems™)

The Applied Biosystems 3730 (48-capillary) Genetic Analyzer was refurbished by the original manufacturer to meet both manufacturing and product specifications. Availability may be limited.

The 48-capillary 3730 DNA Analyzer is the Gold Standard in medium-to-high throughput genetic analysis. Use the 3730 DNA Analyzer for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample. This is upgradeable to 96 capillaries.

• Higher optical sensitivity and advanced polymers enable you to obtain higher-quality data at a lower cost.
• Multiple automation features decrease costly human errors.
• Optimized polymers increase your productivity without compromising your results.
• Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation.

Expand Your Laboratory's Possibilities
By dramatically improving your data quality, significantly reducing your cost per sample, and enabling you to perform more runs per day, the fully automated 48-capillary 3730 system gives your laboratory a tremendous technological and financial advantage. Which means you can pursue an exciting diversity of projects (and you can always upgrade to 96 capillaries when you need to).

Streamline Your Workflow
The 3730 DNA Analyzer system is engineered for highly reliable, unattended operation of up to 48 hours (applies to modules with run times longer than 30 minutes). Automation features which minimize the need for operator intervention and decrease the risk of human error include an integrated plate stacker, internal bar code reader, and onboard polymer delivery system.

Increase Productivity
The high signal-to-noise ratio ensures you get high-quality data – even when you use low-concentration samples and reagents. Labor-saving automation features minimize hands-on time and enable you to analyze more data more efficiently.

Get the Highest-Quality Sequencing and Genotyping Data
The enhanced optical design provides a higher signal-to-noise ratio and a more uniform signal profile across the array. This design, combined with our new, advanced polymer, enables the longest read lengths of any available system, and provides enhanced color balance for streamlined genotyping sample handling. In addition, exceptional sensitivity enables higher success rates across a wider range of sample templates and concentrations than other currently available systems.

One Instrument, Multiple Applications
With the 3730 DNA Analyzer, you get the highest-quality data from a wide array of applications. In addition to being ideal for high-throughput sequencing, the analyzer's optimized application assays, instrument, and analysis software also provide a complete solution for genotyping and resequencing.

Integrated Data Analysis Tools Reduce Time-to-Results
The 3730 DNA Analyzer software suite allows you to generate more meaningful data with less work. This system's labor-saving software suite includes:
• Data Collection (supplied with the instrument): Manages your instrument setup, controls instrument operations, allows real-time data visualization, and performs diagnostics. New features include: Auto-analysis with GeneMapper® and SeqScape®
• Tools to assist with regulatory and compliance requirements (In the United States, this assists with FDA 21CFR part 11).
Flexibility to use any choice in dye set option.
• Additional optimized sequencing run modules covering more applications.
• Sequencing Analysis – Designed to base-call; assign quality values; trim, display, edit and print DNA sequencing data using the KB basecaller
• Seqscape® – Provides everything you need to perform resequencing applications such as VariantSEQr™ Resequencing System
• GeneMapper® – Enables configurable, automated allele calling – a plus for high-throughput genotyping and includes tools for SNPlex™ data analysis

The instrument comes with a one year limited warranty on parts and labor.

For Research Use Only. Not for use in diagnostics procedures.