Shop All Genetic Assay Sample Preparation Kits

QuantiGene™ Sample Processing Kit, cultured cells (Invitrogen™)

This QuantiGene Sample Processing Kit for cultured cells contains reagents and instructions for the preparation of cell lysates for use in QuantiGene Singleplex and QuantiGene Plex assays for RNA targets and QuantiGene Plex DNA assays for DNA targets.

• Easy to follow instructions
• Preserves the integrity of the RNA/DNA without degradation
• Choose the method that matches your cultured cell sample

What you get
The QuantiGene Sample Processing Kit for cultured cells contains our proprietary lysis mixture, proteinase K, and detailed instructions for preparing cell lysates from cultured cells.

Success in cell lysis
Key factors that influence the complete lysis of the cells include:
• The lysis mixture
• Proteinase K
• Vortexing or pipetting of the sample
• Storage of the lysates at -80°C
• Incubation at 50°C

The protocols provided with the QuantiGene Sample Processing Kit for cultured cells use a combination of these factors to ensure complete lysis of the samples. In addition, the lysis mixture provides the proper ionic conditions for the overnight hybridization in the QuantiGene assays.

QuantiGene™ Sample Processing Kit, FFPE samples (Invitrogen™)

The QuantiGene Sample Processing Kit for formalin-fixed paraffin-embedded (FFPE) samples contains reagents and instructions for the preparation of tissue homogenates from FFPE tissue samples for use in QuantiGene Singleplex and QuantiGene Plex assays for RNA or DNA targets. H&E stained slides are compatible with QuantiGene assays.

• Easy to follow instructions
• Preserves the integrity of the RNA/DNA without degradation
• Choose the method that matches your FFPE tissue sample

What you get
The QuantiGene Sample Processing Kit for FFPE samples contains our proprietary homogenizing solution, proteinase K, and detailed instructions for preparing tissue homogenates from FFPE tissue sections.

QuantiGene™ Sample Processing Kit, tissues (Invitrogen™)

The QuantiGene Sample Processing Kit for tissues contains reagents and instructions for the preparation of tissue homogenates from fresh or frozen animal or plant tissues for use in QuantiGene Singleplex and QuantiGene Plex assays for RNA or DNA targets.

• Easy to follow instructions
• Preserves the integrity of the RNA/DNA without degradation
• Choose the method that matches your tissue sample

What you get
The QuantiGene Sample Processing Kit for tissues contains our proprietary homogenizing solution, proteinase K, and detailed instructions for preparing homogenized tissue from a variety of tissue sources.

QuantiGene™ Homogenizing Solution (Invitrogen™)

QuantiGene Homogenizing Solution is used to prepare tissue homogenates from fresh, frozen, or formalin-fixed paraffin-embedded (FFPE) tissues. Homogenization of tissues using QuantiGene Homogenizing Solution releases RNA for detection and quantitation with QuantiGene assays. The homogenizing solution is one of two components in the QuantiGene Sample Processing Kit for tissues, and is used to supplement this kit when extra volume is needed.

Proteinase K (50 µg/µL) (Invitrogen™)

This Proteinase K is used in QuantiGene assays and is one of two components in the QuantiGene Sample Processing kits. Proteinase K digests proteins such as ribonucleases that could interfere with the quantitation of RNA using QuantiGene assays.

QuantiGene™ Sample Processing Kit, blood samples (Invitrogen™)

This QuantiGene Sample Processing Kit for blood samples contains reagents and instructions for the preparation of cell lysates from whole blood samples for use in QuantiGene Singleplex and QuantiGene Plex assays for RNA or DNA targets.

• Easy to follow instructions
• Preserves the integrity of the RNA/DNA without degradation
• Choose the method that matches your whole blood or dried blood spot sample

What you get
The kit contains our proprietary lysis mixture, proteinase K, and detailed instructions for preparing whole blood from heparin, citrate, or EDTA tubes, PAXgene blood RNA tubes, Tempus™ blood RNA tubes, or from dried blood spots prepared from standard anticoagulant venous blood or finger sticks.