Shop All Nucleotides

Bio-11-UTP (75 mM) (Invitrogen™)

Ambion® Biotinylated UTPs are ideal for use as substrates in in vitro transcription reactions. These modified nucleotides can be utilized by a variety of RNA polymerases, including T7, T3, and SP6 RNA polymerases, as well as other RNA modifying enzymes. It is provided in one tube containing 30 µL (2,250 nmol). Biotinylated RNA can be used in place of radioactively labeled RNA in many applications, including cRNA amplification and labeling for microarray target preparation. Detection is then carried out via one of a variety of streptavidin-based methods. This Bio-UTP is confirmed free of non-specific endonuclease activity, exonuclease activity, and RNase activity and is functionally tested using the MessageAmp™ II aRNA Amplification Kit (SKU# AM1751).

Bio-16-UTP (10 mM) (Invitrogen™)

Ambion® Biotinylated UTPs are ideal for use as substrates in in vitro transcription reactions. These modified nucleotides can be utilized by a variety of RNA polymerases, including T7, T3, and SP6 RNA polymerases, as well as other RNA modifying enzymes. It is provided in one tube containing 25 μL (250 nmol). Biotinylated RNA can be used in place of radioactively labeled RNA in many applications, including cRNA amplification and labeling for microarray target preparation. Detection is then carried out via one of a variety of streptavidin-based methods. This Bio-UTP is confirmed free of non-specific endonuclease activity, exonuclease activity, and RNase activity and is functionally tested using the MessageAmp™ II aRNA Amplification Kit (SKU# AM1751).

GeneAmp™ dNTP Blend (2.5 mM ea) (Applied Biosystems™)

GeneAmp® dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. Each vial consists of 1 mL of 2.5 mM each of dATP, dCTP, dGTP, and dTTP, yielding a 10 mM blend of dNTPs. Key advantages of GeneAmp® dNTPs:

• dNTPs are of high purity, making them ideal for use in PCR reactions or other applications
• Guaranteed for PCR performance when used with GeneAmp® PCR core reagents and GeneAmp® instrument systems
• Premixed dNTP blends are available in various concentrations, offering ease of use

MANT-ATP (2'-(or-3')-O-(N-Methylanthraniloyl) Adenosine 5'-Triphosphate, Trisodium Salt) (Invitrogen™)

The nucleotide analog MANT ATP is modified on the ribose moiety. The compact mature of the MANT fluorophore and its attachment position results in nucleotide analogs that induce minimal perturbation of nucleotide-protein interactions. Because MANT fluorescence is sensitive to the environment of the fluorophore, nucleotide-protein interactions may be directly detectable. MANT nucleotides are valuable probes of the structure and enzymatic activity of nucleotide-binding proteins.

GeneAmp™ dNTP Blend (100 mM) (Applied Biosystems™)

GeneAmp® dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH.

dNTPs have high purity, making them ideal for use in PCR reactions or other applications

• Guaranteed for PCR performance when used with GeneAmp® PCR core reagents and GeneAmp® instrument systems
• Premixed dNTP blends are available in various concentrations, offering ease of use

GeneAmp® dNTP Blend (100 mM) contains 1 mL of a 100 mM solution of dNTP (25 mM each of dATP, dCTP, dGTP and dTTP). Sufficient for 100 PCR amplifications, 100 µL each.

Alexa Fluor™ 555-aha-dCTP (Invitrogen™)

The Alexa Fluor® 555 dyes, used to label nucleotides, are compatible with commonly used microarray scanners, and provide greater signal correlation (R2) valuesthan the spectrally similar Cy™3 and Cy™5 dye pair, improving the resolution of two-color microarray gene expression assays to 1.3-fold changes in expression. The exceptionally bright and photostable Alexa Fluor® dyes are also essentially insensitive tob pH and are highly water soluble.

dNTP Mix (2.5 mM) (Invitrogen™)

The same high-quality deoxynucleotide triphosphate solutions provided in the Ambion® RETROscript® First Strand cDNA Synthesis Kit for RT-PCR (SKU# AM1710). They are subject to rigorous nuclease testing as well as a functional assay to ensure high levels of incorporation. Supplied in one tube containing 0.3 mL, premixed at a concentration of 2.5 mM each.

ATP (Invitrogen™)

ATP, CTP, GTP, UTP (nucleoside 5´-triphosphates), and 10 mM NTP Mix are suitable for use in in vitro transcription reactions with SP6, T3, and T7 RNA polymerases for production of RNA probes, or RNA transcripts for translation in in vitro translation extracts. These nucleotides are supplied as convenient, ready-to-use solutions at a concentration of 10 mM in 1 mM Tris-HCl (pH 7.5).

Fluorescein-aha-dUTP - 1 mM in TE Buffer (Invitrogen™)

Labeled 5-aminohexylacrylamido-dUTP (aha-dUTP) nucleotides are modified at the C-5 position of uridine with a unique hexylacrylamide linker, which serves as a spacer between the nucleotide and the dye. This spacer reduces interactions between the nucleotide and the dye, resulting in brighter conjugates and increased hapten accessibility for secondary detection reagents. Labeled nucleotides can be used to generate labeled nucleic acid hybridization probes for many molecular biology and molecular cytogenetics applications, including multicolor techniques.

Aminoallyl-dUTP Solution (50 mM) (Thermo Scientific™)

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.

Applications

• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension

dCTP Solution (100 mM) (Thermo Scientific™)

Thermo Scientific dCTP (2'-deoxycytidine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. The nucleotide has greater than 99% purity, is free of nuclease activities, human and E. coli DNA. It is designed for many different molecular biology applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of human and E.coli DNA
• Highly stable

Application tested in:
• Long range PCR (40 kb)
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR
• High fidelity PCR

Applications

For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

GeneAmp™ dNTP Blend with dUTP (12.5 mM) (Applied Biosystems™)

GeneAmp® dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. GeneAmp® dNTPs with dUTP can be used to generate dU-containing PCR products in a carry-over prevention strategy in conjunction with AmpErase ® Uracil N-glycosylase (UNG).

• dNTPs have high purity, making them ideal for use in PCR reactions or other applications.
• dNTPs with dUTP are optimized for use in carry-over prevention.
• Guaranteed for PCR performance when used with GeneAmp® PCR core reagents and GeneAmp® instrument systems.
• Premixed dNTP blends are available in various concentrations, offering ease of use.

GeneAmp® dNTP Blend, 12.5 mM with dUTP contains 1 mL of a 12.5 mM solution of dNTP (2.5 mM each of dATP, dCTP, dGTP and 5.0 mM of dUTP). Sufficient for 100 PCR amplifications, 100 µL each

The PCR process is covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd.

For Research Use Only. Not for use in diagnostics procedures.

Alexa Fluor™ 647-aha-dCTP (Invitrogen™)

The Alexa Fluor® 647 dyes, used to label nucleotides, are compatible with commonly used microarray scanners, and provide greater signal correlation (R2) valuesthan the spectrally similar Cy™3 and Cy™5 dye pair, improving the resolution of two-color microarray gene expression assays to 1.3-fold changes in expression. The exceptionally bright and photostable Alexa Fluor® dyes are also essentially insensitive tob pH and are highly water soluble.

NTP Mix (10 mM) (Invitrogen™)

ATP, CTP, GTP, UTP (nucleoside 5´-triphosphates), and 10 mM NTP Mix are suitable for use in in vitro transcription reactions with SP6, T3, and T7 RNA polymerases for production of RNA probes, or RNA transcripts for translation in in vitro translation extracts. These nucleotides are supplied as convenient, ready-to-use solutions at a concentration of 10 mM in 1 mM Tris-HCl (pH 7.5).

EdU (5-ethynyl-2’-deoxyuridine) (Invitrogen™)

EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows for more reproducible results, a simpler and quicker protocol and measurements which can easily be multiplexed with relevant antibody based targets including phospho-histone H3, Ki-67, and cyclin B1 as well as dual-pulse experiments with BrdU by flow cytometry, fluorescence microscopy, microplate (HTS) or high-throughput imaging (HCS).

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.