Shop All Nucleotides

NTP Set, Tris buffered (Thermo Scientific™)

Thermo Scientific NTP Set, Tris buffered is a convenient set of 100 mM solutions of each of Tris buffered ATP, CTP, GTP and UTP. These extremely stable nucleotides are supplied as aqueous solutions titrated to pH 7.3-7.5 with Tris base. The nucleotides have greater than 99% purity, are free of nuclease activities and functionally tested in in vitro transcription.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of trace contaminating nucleotides, endo-, exodeoxyribonuclease and ribonuclease activities
• Higher in vitro transcription yields using Tris buffered NTPs in comparison to NTPs, titrated with NaOH
• High stability—stable for 3 years at -20°C and after multiple freeze-thaw cycles

Applications

• in vitro transcription
• RNA amplification
• siRNA synthesis
• aRNA synthesis

EU (5-Ethynyl Uridine) (Invitrogen™)

Detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide, ethynyl uridine (EU, E10345) or in kit form with Click-iT Nascent RNA capture kit, C-10365.
Both approaches use powerful click chemistry detection, a simple and robust two-step technique. In step one, the alkyne-containing biomolecule is fed to cells or animals and actively incorporated into RNA. Detection utilizes the chemoselective ligation or “click" reaction between an azide and an alkyne where the modified protein is detected with a corresponding azide-containing dye or hapten using the Click-iT® Cell Reaction Buffer Kit. Cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis (HCS).
By using the Click-iT Nascent RNA capture kit, the nascent pool of RNA can be isolated for transcriptome analysis, including RNAseq, ΔCT, RNA turnover, Nuclear run and run off, array analysis and more. Please see this link for more details on the Click-iT Nascent RNA capture kit: Click here.

Alexa Fluor™ 555-aha-dUTP, 1 mM in TE buffer (Invitrogen™)

Labeled 5-aminohexylacrylamido-dUTP (aha-dUTP) nucleotides are modified at the C-5 position of uridine with a unique hexylacrylamide linker, which serves as a spacer between the nucleotide and the dye. This spacer reduces interactions between the nucleotide and the dye, resulting in brighter conjugates and increased hapten accessibility for secondary detection reagents. Labeled nucleotides can be used to generate labeled nucleic acid hybridization probes for many molecular biology and molecular cytogenetics applications, including multicolor techniques.

5-(3-Aminoallyl)-dUTP (50 mM) (Invitrogen™)

When incorporated into DNA, 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.

Use of this Modified Nucleotide:
Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription. Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals.

dATP (10 mM) (Invitrogen™)

Invitrogen dATP is prepared to a final concentration of 10 mM in 0.9 mM Tris-HCl. 10 mM dATP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Invitrogen dNTP manufacturing meets the highest industry standards:
• Manufactured under ISO 9001
• Manufactured in Class D clean rooms

Features include:
• Chemically synthesized
• pH 7.5
• >99% purity confirmed by HPLC
• Stable for 2 years at –20°C
• Free from qPCR, PCR, reverse transcription inhibitors
• Free of DNases and RNases
• Free of human and E. coli DNA

Applications:
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA synthesis
• High-fidelity and long-range PCR
• Isothermal amplification
• DNA labeling
• Cloning
• Sanger and next-generation sequencing (NGS) sequencing

dNTPs in a different concentration or format ›

ChromaTide™ Fluorescein-12-dUTP (Invitrogen™)

Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis.

ChromaTide® Labeled Nucleotides Specifications:
• Ex/Em of dye: fluorescein-12-dUTP (495/525 nm)
• Length of alkynylamino linker: 12 atoms


Methods for Incorporating ChromaTide® Nucleotides Into Probes
• Nick translation
• Random primer labeling
• End-labeling with terminal deoxynucleotidyl transferase
• Reverse transcription
• PCR amplification


See Methods for Enzymatic Incorporation of ChromaTide® dUTPs for specific guidelines for each of these methods.

Alexa Fluor® and BODIPY® Fluorescent Dyes Make Excellent Probes
Probes made with labeled nucleotides can be used for multicolor techniques such as in situ hybridization and hybridization to arrays. Our proprietary BODIPY® and Alexa Fluor® dye conjugates are exceptionally bright, photostable, and essentially pH insensitive. The narrow emission profile of the BODIPY® dyes helps ensure minimal spectral overlap. The Alexa Fluor® dyes are highly water soluble, as are DNA probes made from them, making them the labels of choice for fluorescence in situ hybridization.

Long Linkers Improve Performance
The ChromaTide® dUTP and UTP nucleotides are modified at the C-5 position of uridine via a unique alkynylamino linker, which provides a spacer between the nucleotide and the dye to reduce interactions between them. The number in the product name, e.g., the “12” in fluorescein-12-dUTP, indicates the net length of the spacer, in atoms. These spacers result in brighter conjugates and increased hapten accessibility for secondary detection reagents.

For complete listing of our ChromaTide® Reagents: Molecular Probes ChromaTide® and aha labeled nucleotides—Table 8.5.
For additional information on these labeling reagents, read Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

CTP (Invitrogen™)

ATP, CTP, GTP, UTP (nucleoside 5´-triphosphates), and 10 mM NTP Mix are suitable for use in in vitro transcription reactions with SP6, T3, and T7 RNA polymerases for production of RNA probes, or RNA transcripts for translation in in vitro translation extracts. These nucleotides are supplied as convenient, ready-to-use solutions at a concentration of 10 mM in 1 mM Tris-HCl (pH 7.5).

ATP Solution (100 mM) (Thermo Scientific™)

Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. This nucleotide is used in different molecular biology applications, and it has greater than 99% purity.

Highlights
• Greater than 99% purity confirmed by HPLC
• Functionally tested in in vitro transcription
• High stability – stable for two years at -20°C and after multiple freeze-thaw cycles

Applications
in vitro transcription
• aRNA synthesis
• siRNA synthesis
• RNA amplification
• Ligation
• Phosphorylation

MANT-GTP (2'-(or-3')-O-(N-Methylanthraniloyl) Guanosine 5'-Triphosphate, Trisodium Salt) (Invitrogen™)

The nucleotide analog MANT GTP is modified on the ribose moiety. The compact mature of the MANT fluorophore and its attachment position results in nucleotide analogs that induce minimal perturbation of nucleotide-protein interactions. Because MANT fluorescence is sensitive to the environment of the fluorophore, nucleotide-protein interactions may be directly detectable. MANT nucleotides are valuable probes of the structure and enzymatic activity of nucleotide-binding proteins.

Alexa Fluor™ 647 ATP (Adenosine 5'-Triphosphate, Alexa Fluor™ 647 2'-(or-3')-O-(N-(2-Aminoethyl) Urethane), Hexa(Triethylammonium) Salt) (Invitrogen™)

Alexa Fluor® 647 adenosine 5´triphosphate (Alexa Fluor® 647 ATP) is a mixed-isomer comprised of the Alexa Fluor® 647 fluorophore, an exceptionally bright far-red fluorescent dye attached to the 2´ or 3´ position of the ribose ring via an aminoethylcarbamoyl linker.

dNTP Set, 100 mM Solutions (Thermo Scientific™)

Thermo Scientific dNTP Set is a convenient set of 100 mM aqueous solutions of each of dATP, dCTP, dGTP and dTTP, supplied in separate vials. The solutions of these extremely stable nucleotides are titrated to pH 7.3-7.5 with NaOH. The nucleotides have greater than 99% purity, are free of nuclease activities, human and E. coli DNA. They are designed for many different molecular biology applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA

Application tested in:
• Long range PCR (40 kb)
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR
• High fidelity PCR

Highly stable
• Stable after multiple freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 min at 72°C)

Applications

• For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

Alexa Fluor™ 647-aha-dUTP, 1 mM in TE buffer (Invitrogen™)

Labeled 5-aminohexylacrylamido-dUTP (aha-dUTP) nucleotides are modified at the C-5 position of uridine with a unique hexylacrylamide linker, which serves as a spacer between the nucleotide and the dye. This spacer reduces interactions between the nucleotide and the dye, resulting in brighter conjugates and increased hapten accessibility for secondary detection reagents. Labeled nucleotides can be used to generate labeled nucleic acid hybridization probes for many molecular biology and molecular cytogenetics applications, including multicolor techniques.

Biotin-14-dCTP (Invitrogen™)

Biotin-14-dCTP is used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling. Advantages of using biotinylated DNA probes include:

• Convenience and safety—probe preparation generates no radioactive waste
• Stability—probes remain active longer than isotopically labeled probes
• Versatility—biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding

About Biotin-14-dCTP
Biotin-14-dCTP is a patented dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, and dTTP, labeling DNA with terminal deoxynucleotide transferase, or random primer synthesis. The amount of material provided is sufficient for labeling up to 50 µg of DNA.

Performance and quality testing
Purity is evaluated by reverse-phase HPLC.

BODIPY™ FL GDP (Guanosine 5'-Diphosphate, BODIPY™ FL 2'-(or-3')-O-(N-(2-Aminoethyl) Urethane), Bis (Triethylammonium) Salt) (Invitrogen™)

The mixed-isomer analog of BODIPY® FL GDP is comprised of the BODIPY® FL fluorophore attached to the 2’ or 3’ position of the ribose ring via an aminoethylcarbamoyl linker. BODIPY® FL exhibits spectral characteristics similar to fluorescein (FITC) or the Alexa Fluor® 488 dye.

Bio-11-UTP (10 mM) (Invitrogen™)

Ambion® Biotinylated UTPs are ideal for use as substrates in in vitro transcription reactions. These modified nucleotides can be utilized by a variety of RNA polymerases, including T7, T3, and SP6 RNA polymerases, as well as other RNA modifying enzymes. It is provided in one tube containing 25 μL (250 nmol). Biotinylated RNA can be used in place of radioactively labeled RNA in many applications, including cRNA amplification and labeling for microarray target preparation. Detection is then carried out via one of a variety of streptavidin-based methods. This Bio-UTP is confirmed free of non-specific endonuclease activity, exonuclease activity, and RNase activity and is functionally tested using the MessageAmp™ II aRNA Amplification Kit (SKU# AM1751).