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CTP Solution (100 mM) (Thermo Scientific™)

Thermo Scientific CTP (cytidine 5'-triphosphate) is an extremely stable nucleotide with more than 99% purity. It is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. This nucleotide is designed for a variety of molecular biology applications.

Highlights
• Greater than 99% purity confirmed by HPLC
• Functionally tested in in vitro transcription
• High stability – stable for two years at -20°C and after multiple freeze-thaw cycles

Applications
• aRNA synthesis
• siRNA synthesis
• RNA amplification
in vitro transcription

dNTP Mix (2 mM each) (Thermo Scientific™)

Thermo Scientific dNTP Mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 2 mM. The nucleotides have greater than 99% purity, are free of nuclease activities, human and E. coli DNA. Mixes offer the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. They are designed for many different molecular biology applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA
• Highly stable
• Stable for years at -20°C
• Stable after multiple freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C; 3 minutes at 72°C)

Application tested in:
• Long range PCR (40 kb)
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR
• High fidelity PCR

Applications
• For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

GTP Solution (100 mM) (Thermo Scientific™)

Thermo Scientific GTP (guanosine 5'-triphosphate) is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. It is more than 99% pure and highly stable. You can use GTP in different molecular biology applications.

Highlights
• Greater than 99% purity confirmed by HPLC
• Functionally tested in in vitro transcription
• High stability – stable for two years at -20°C and after multiple freeze-thaw cycles

Applications
• aRNA synthesis
• siRNA synthesis
• RNA amplification
in vitro transcription

Bio-11-UTP (75 mM) (Invitrogen™)

Ambion® Biotinylated UTPs are ideal for use as substrates in in vitro transcription reactions. These modified nucleotides can be utilized by a variety of RNA polymerases, including T7, T3, and SP6 RNA polymerases, as well as other RNA modifying enzymes. It is provided in one tube containing 30 µL (2,250 nmol). Biotinylated RNA can be used in place of radioactively labeled RNA in many applications, including cRNA amplification and labeling for microarray target preparation. Detection is then carried out via one of a variety of streptavidin-based methods. This Bio-UTP is confirmed free of non-specific endonuclease activity, exonuclease activity, and RNase activity and is functionally tested using the MessageAmp™ II aRNA Amplification Kit (SKU# AM1751).

ATP Solution (10 mM)

ATP is an essential component for kinase assays using LanthaScreen® Technology. Invitrogen's ATP is 10mM in water.

UTP Solution (100 mM) (Thermo Scientific™)

Thermo Scientific UTP (uridine 5'-triphosphate) is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. This highly stable nucleotide is more than 99% pure. You can use UTP in many molecular biology applications.

Highlights
• Greater than 99% purity confirmed by HPLC
• Functionally tested in in vitro transcription
• High stability – stable for two years at -20°C and after multiple freeze-thaw cycles

Applications
• aRNA synthesis
• siRNA synthesis
• RNA amplification
in vitro transcription

Bio-16-UTP (10 mM) (Invitrogen™)

Ambion® Biotinylated UTPs are ideal for use as substrates in in vitro transcription reactions. These modified nucleotides can be utilized by a variety of RNA polymerases, including T7, T3, and SP6 RNA polymerases, as well as other RNA modifying enzymes. It is provided in one tube containing 25 μL (250 nmol). Biotinylated RNA can be used in place of radioactively labeled RNA in many applications, including cRNA amplification and labeling for microarray target preparation. Detection is then carried out via one of a variety of streptavidin-based methods. This Bio-UTP is confirmed free of non-specific endonuclease activity, exonuclease activity, and RNase activity and is functionally tested using the MessageAmp™ II aRNA Amplification Kit (SKU# AM1751).

NTP Set, 100 mM Solution (Thermo Scientific™)

The Thermo Scientific NTP set is a convenient set of 100 mM solutions of each ATP, CTP, GTP and UTP. These highly stable nucleotides are more than 99% pure, and can be used in a variety of molecular biology applications.

Highlights
• Greater than 99% purity confirmed by HPLC
• Functionally tested in in vitro transcription
• High stability – stable for two years at -20°C and after multiple freeze-thaw cycles

Applications
• aRNA synthesis
• siRNA synthesis
• RNA amplification
in vitro transcription

EU (5-Ethynyl Uridine) (Invitrogen™)

Detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide, ethynyl uridine (EU, E10345) or in kit form with Click-iT Nascent RNA capture kit, C-10365.
Both approaches use powerful click chemistry detection, a simple and robust two-step technique. In step one, the alkyne-containing biomolecule is fed to cells or animals and actively incorporated into RNA. Detection utilizes the chemoselective ligation or “click" reaction between an azide and an alkyne where the modified protein is detected with a corresponding azide-containing dye or hapten using the Click-iT® Cell Reaction Buffer Kit. Cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis (HCS).
By using the Click-iT Nascent RNA capture kit, the nascent pool of RNA can be isolated for transcriptome analysis, including RNAseq, ΔCT, RNA turnover, Nuclear run and run off, array analysis and more. Please see this link for more details on the Click-iT Nascent RNA capture kit: Click here.

GTP Soln. (10 mM) (Invitrogen™)

This Ambion® ribonucleotide triphosphate (rNTP) solution is a component of the MAXIscript® Kits (SKU#s AM1312, AM1316, AM1314, AM1326, AM1324, AM1318, AM1322, AM1320, AM1310, and AM1308) and is supplied in one tube containing 100 μL. Each is subjected to RNase testing as well as a functional assay to ensure high levels of incorporation.

CTP (Invitrogen™)

ATP, CTP, GTP, UTP (nucleoside 5´-triphosphates), and 10 mM NTP Mix are suitable for use in in vitro transcription reactions with SP6, T3, and T7 RNA polymerases for production of RNA probes, or RNA transcripts for translation in in vitro translation extracts. These nucleotides are supplied as convenient, ready-to-use solutions at a concentration of 10 mM in 1 mM Tris-HCl (pH 7.5).

dATP (10 mM) (Invitrogen™)

Invitrogen dATP is prepared to a final concentration of 10 mM in 0.9 mM Tris-HCl. 10 mM dATP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Invitrogen dNTP manufacturing meets the highest industry standards:
• Manufactured under ISO 9001
• Manufactured in Class D clean rooms

Features include:
• Chemically synthesized
• pH 7.5
• >99% purity confirmed by HPLC
• Stable for 2 years at –20°C
• Free from qPCR, PCR, reverse transcription inhibitors
• Free of DNases and RNases
• Free of human and E. coli DNA

Applications:
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA synthesis
• High-fidelity and long-range PCR
• Isothermal amplification
• DNA labeling
• Cloning
• Sanger and next-generation sequencing (NGS) sequencing

dNTPs in a different concentration or format ›

ChromaTide™ Texas Red™-12-dUTP (Invitrogen™)

Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis.

ChromaTide® Labeled Nucleotides Specifications:
• Ex/Em of dye: Texas Red®-12-dUTP (595/615 nm)
• Length of alkynylamino linker: 12 atoms


Methods for Incorporating ChromaTide® Nucleotides Into Probes
• Nick translation
• Random primer labeling
• End-labeling with terminal deoxynucleotidyl transferase
• Reverse transcription
• PCR amplification


See Methods for Enzymatic Incorporation of ChromaTide® dUTPs for specific guidelines for each of these methods.

Alexa Fluor® and BODIPY® Fluorescent Dyes Make Excellent Probes
Probes made with labeled nucleotides can be used for multicolor techniques such as in situ hybridization and hybridization to arrays. Our proprietary BODIPY® and Alexa Fluor® dye conjugates are exceptionally bright, photostable, and essentially pH insensitive. The narrow emission profile of the BODIPY® dyes helps ensure minimal spectral overlap. The Alexa Fluor® dyes are highly water soluble, as are DNA probes made from them, making them the labels of choice for fluorescence in situ hybridization.

Long Linkers Improve Performance
The ChromaTide® dUTP and UTP nucleotides are modified at the C-5 position of uridine via a unique alkynylamino linker, which provides a spacer between the nucleotide and the dye to reduce interactions between them. The number in the product name, e.g., the “12” in fluorescein-12-dUTP, indicates the net length of the spacer, in atoms. These spacers result in brighter conjugates and increased hapten accessibility for secondary detection reagents.

For complete listing of our ChromaTide® Reagents: Molecular Probes ChromaTide® and aha labeled nucleotides—Table 8.5.
For additional information on these labeling reagents, read Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

dCTP Solution (100 mM) (Thermo Scientific™)

Thermo Scientific dCTP (2'-deoxycytidine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. The nucleotide has greater than 99% purity, is free of nuclease activities, human and E. coli DNA. It is designed for many different molecular biology applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of human and E.coli DNA
• Highly stable

Application tested in:
• Long range PCR (40 kb)
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR
• High fidelity PCR

Applications

For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

UTP Soln. (10 mM) (Invitrogen™)

This Ambion® ribonucleotide triphosphate (rNTP) solution is a component of the MAXIscript® Kits (SKU#s AM1312, AM1316, AM1314, AM1326, AM1324, AM1318,#AM1322, AM1320, AM1310, and AM1308) and is supplied in one tube containing 100 μL. Each is subjected to RNase testing as well as a functional assay to ensure high levels of incorporation.