Shop All Fluorescently Labeled Nucleotides

MANT-ATP (2'-(or-3')-O-(N-Methylanthraniloyl) Adenosine 5'-Triphosphate, Trisodium Salt) (Invitrogen™)

The nucleotide analog MANT ATP is modified on the ribose moiety. The compact mature of the MANT fluorophore and its attachment position results in nucleotide analogs that induce minimal perturbation of nucleotide-protein interactions. Because MANT fluorescence is sensitive to the environment of the fluorophore, nucleotide-protein interactions may be directly detectable. MANT nucleotides are valuable probes of the structure and enzymatic activity of nucleotide-binding proteins.

Alexa Fluor™ 555-aha-dCTP (Invitrogen™)

The Alexa Fluor® 555 dyes, used to label nucleotides, are compatible with commonly used microarray scanners, and provide greater signal correlation (R2) valuesthan the spectrally similar Cy™3 and Cy™5 dye pair, improving the resolution of two-color microarray gene expression assays to 1.3-fold changes in expression. The exceptionally bright and photostable Alexa Fluor® dyes are also essentially insensitive tob pH and are highly water soluble.

Fluorescein-aha-dUTP - 1 mM in TE Buffer (Invitrogen™)

Labeled 5-aminohexylacrylamido-dUTP (aha-dUTP) nucleotides are modified at the C-5 position of uridine with a unique hexylacrylamide linker, which serves as a spacer between the nucleotide and the dye. This spacer reduces interactions between the nucleotide and the dye, resulting in brighter conjugates and increased hapten accessibility for secondary detection reagents. Labeled nucleotides can be used to generate labeled nucleic acid hybridization probes for many molecular biology and molecular cytogenetics applications, including multicolor techniques.

Alexa Fluor™ 647-aha-dCTP (Invitrogen™)

The Alexa Fluor® 647 dyes, used to label nucleotides, are compatible with commonly used microarray scanners, and provide greater signal correlation (R2) valuesthan the spectrally similar Cy™3 and Cy™5 dye pair, improving the resolution of two-color microarray gene expression assays to 1.3-fold changes in expression. The exceptionally bright and photostable Alexa Fluor® dyes are also essentially insensitive tob pH and are highly water soluble.

Guanosine 5'-O-(3-Thiotriphosphate), BODIPY™ FL Thioester, Sodium Salt (BODIPY™ FL GTP-γ-S, Thioester) (Invitrogen™)

BODIPY FL GTP-γ-S binds to GTP-binding proteins. To create this fluorescent nucleotide, the BODIPY FL fluorophore was linked through the γ-thiol of GTP-γ-S. The fluorescence of BODIPY FL GTP-γ-S is quenched ~90% relative to that of the free dye, but may be recovered on binding to G-proteins. In addition to its potential use for binding studies, the BODIPY FL GTP-γ-S thioether is an important new substrate for Fhit, a member of the histidine triad superfamily of nucleotide-binding proteins.

BODIPY™ FL ATP (Adenosine 5´-Triphosphate, BODIPY™ FL 2´-(or-3´)-O-(N-(2-Aminoethyl)Urethane), Trisodium Salt), 5 mM in buffer (Invitrogen™)

BODIPY FL adenosine 5'-triphosphate and BODIPY FL guanosine 5'-triphosphate (G-12411) are composed of a BODIPY FL fluorophore attached to the 2' or 3' position of the ribose ring via a linker. Interactions between the fluorophore and the purine base are evident from the spectroscopic properties of these nucleotide analogs. The fluorescence quantum yield of BODIPY FL GTP is only about one-tenth that of BODIPY FL ATP; however, this yield may be enhanced upon binding of BODIPY FL GTP to GTP-binding proteins.

Fluorescein-12-dUTP Solution (1 mM) (Thermo Scientific™)

Thermo Scientific Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution. Fluorescein-12-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment and DNA Polymerase I.

Applications

• Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.

BODIPY™ FL GDP (Guanosine 5'-Diphosphate, BODIPY™ FL 2'-(or-3')-O-(N-(2-Aminoethyl) Urethane), Bis (Triethylammonium) Salt) (Invitrogen™)

The mixed-isomer analog of BODIPY® FL GDP is comprised of the BODIPY® FL fluorophore attached to the 2’ or 3’ position of the ribose ring via an aminoethylcarbamoyl linker. BODIPY® FL exhibits spectral characteristics similar to fluorescein (FITC) or the Alexa Fluor® 488 dye.

Alexa Fluor™ 555-aha-dUTP, 1 mM in TE buffer (Invitrogen™)

Labeled 5-aminohexylacrylamido-dUTP (aha-dUTP) nucleotides are modified at the C-5 position of uridine with a unique hexylacrylamide linker, which serves as a spacer between the nucleotide and the dye. This spacer reduces interactions between the nucleotide and the dye, resulting in brighter conjugates and increased hapten accessibility for secondary detection reagents. Labeled nucleotides can be used to generate labeled nucleic acid hybridization probes for many molecular biology and molecular cytogenetics applications, including multicolor techniques.

MANT-GTP (2'-(or-3')-O-(N-Methylanthraniloyl) Guanosine 5'-Triphosphate, Trisodium Salt) (Invitrogen™)

The nucleotide analog MANT GTP is modified on the ribose moiety. The compact mature of the MANT fluorophore and its attachment position results in nucleotide analogs that induce minimal perturbation of nucleotide-protein interactions. Because MANT fluorescence is sensitive to the environment of the fluorophore, nucleotide-protein interactions may be directly detectable. MANT nucleotides are valuable probes of the structure and enzymatic activity of nucleotide-binding proteins.

ChromaTide™ Alexa Fluor™ 488-5-UTP (Invitrogen™)

Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis.

ChromaTide® Labeled Nucleotides Specifications:
• Ex/Em of dye: Alexa Fluor® 488-5-UTP (490/520 nm)
• Length of alkynylamino linker: 5 atoms


Methods for Incorporating ChromaTide® Nucleotides Into Probes
• Nick translation
• Random primer labeling
• End-labeling with terminal deoxynucleotidyl transferase
• Reverse transcription
• PCR amplification


See Methods for Enzymatic Incorporation of ChromaTide® dUTPs for specific guidelines for each of these methods.

Alexa Fluor® and BODIPY® Fluorescent Dyes Make Excellent Probes
Probes made with labeled nucleotides can be used for multicolor techniques such as in situ hybridization and hybridization to arrays. Our proprietary BODIPY® and Alexa Fluor® dye conjugates are exceptionally bright, photostable, and essentially pH insensitive. The narrow emission profile of the BODIPY® dyes helps ensure minimal spectral overlap. The Alexa Fluor® dyes are highly water soluble, as are DNA probes made from them, making them the labels of choice for fluorescence in situ hybridization.

Long Linkers Improve Performance
The ChromaTide® dUTP and UTP nucleotides are modified at the C-5 position of uridine via a unique alkynylamino linker, which provides a spacer between the nucleotide and the dye to reduce interactions between them. The number in the product name, e.g., the “12” in fluorescein-12-dUTP, indicates the net length of the spacer, in atoms. These spacers result in brighter conjugates and increased hapten accessibility for secondary detection reagents.

For complete listing of our ChromaTide® Reagents: Molecular Probes ChromaTide® and aha labeled nucleotides—Table 8.5.
For additional information on these labeling reagents, read Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

ChromaTide™ Alexa Fluor™ 594-5-dUTP (Invitrogen™)

Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis.

ChromaTide® Labeled Nucleotides Specifications:
• Ex/Em of dye: Alexa Fluor® 594-5-dUTP (590/615 nm)
• Length of alkynylamino linker: 5 atoms


Methods for Incorporating ChromaTide® Nucleotides Into Probes
• Nick translation
• Random primer labeling
• End-labeling with terminal deoxynucleotidyl transferase
• Reverse transcription
• PCR amplification


See Methods for Enzymatic Incorporation of ChromaTide® dUTPs for specific guidelines for each of these methods.

Alexa Fluor® and BODIPY® Fluorescent Dyes Make Excellent Probes
Probes made with labeled nucleotides can be used for multicolor techniques such as in situ hybridization and hybridization to arrays. Our proprietary BODIPY® and Alexa Fluor® dye conjugates are exceptionally bright, photostable, and essentially pH insensitive. The narrow emission profile of the BODIPY® dyes helps ensure minimal spectral overlap. The Alexa Fluor® dyes are highly water soluble, as are DNA probes made from them, making them the labels of choice for fluorescence in situ hybridization.

Long Linkers Improve Performance
The ChromaTide® dUTP and UTP nucleotides are modified at the C-5 position of uridine via a unique alkynylamino linker, which provides a spacer between the nucleotide and the dye to reduce interactions between them. The number in the product name, e.g., the “12” in fluorescein-12-dUTP, indicates the net length of the spacer, in atoms. These spacers result in brighter conjugates and increased hapten accessibility for secondary detection reagents.

For complete listing of our ChromaTide® Reagents: Molecular Probes ChromaTide® and aha labeled nucleotides—Table 8.5.
For additional information on these labeling reagents, read Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

ChromaTide™ Alexa Fluor™ 488-5-dUTP (Invitrogen™)

Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis.

ChromaTide® Labeled Nucleotides Specifications:
• Ex/Em of dye: Alexa Fluor® 488-5-dUTP (490/520 nm)
• Length of alkynylamino linker: 5 atoms


Methods for Incorporating ChromaTide® Nucleotides Into Probes
• Nick translation
• Random primer labeling
• End-labeling with terminal deoxynucleotidyl transferase
• Reverse transcription
• PCR amplification


See Methods for Enzymatic Incorporation of ChromaTide® dUTPs for specific guidelines for each of these methods.

Alexa Fluor® and BODIPY® Fluorescent Dyes Make Excellent Probes
Probes made with labeled nucleotides can be used for multicolor techniques such as in situ hybridization and hybridization to arrays. Our proprietary BODIPY® and Alexa Fluor® dye conjugates are exceptionally bright, photostable, and essentially pH insensitive. The narrow emission profile of the BODIPY® dyes helps ensure minimal spectral overlap. The Alexa Fluor® dyes are highly water soluble, as are DNA probes made from them, making them the labels of choice for fluorescence in situ hybridization.

Long Linkers Improve Performance
The ChromaTide® dUTP and UTP nucleotides are modified at the C-5 position of uridine via a unique alkynylamino linker, which provides a spacer between the nucleotide and the dye to reduce interactions between them. The number in the product name, e.g., the “12” in fluorescein-12-dUTP, indicates the net length of the spacer, in atoms. These spacers result in brighter conjugates and increased hapten accessibility for secondary detection reagents.

For complete listing of our ChromaTide® Reagents: Molecular Probes ChromaTide® and aha labeled nucleotides—Table 8.5.
For additional information on these labeling reagents, read Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

BODIPY™ FL ATP-γ-S, Thioester (Adenosine 5'-O-(3-Thiotriphosphate), BODIPY™ FL Thioester, Sodium Salt) (Invitrogen™)

BODIPY® FL ATP-γ-S binds to ATP-binding proteins. To create this fluorescent nucleotide, the BODIPY FL fluorophore was linked through the γ-thiol of ATP-γ-S. In addition to its potential use for binding studies, the BODIPY FL ATP-γ-S thioether is an important new substrate for Fhit, a member of the histidine triad superfamily of nucleotide-binding proteins.

Alexa Fluor™ 647-aha-dUTP, 1 mM in TE buffer (Invitrogen™)

Labeled 5-aminohexylacrylamido-dUTP (aha-dUTP) nucleotides are modified at the C-5 position of uridine with a unique hexylacrylamide linker, which serves as a spacer between the nucleotide and the dye. This spacer reduces interactions between the nucleotide and the dye, resulting in brighter conjugates and increased hapten accessibility for secondary detection reagents. Labeled nucleotides can be used to generate labeled nucleic acid hybridization probes for many molecular biology and molecular cytogenetics applications, including multicolor techniques.