Shop All Biotin Labeled Nucleotides

Bio-11-UTP (10 mM) (Invitrogen™)

Ambion® Biotinylated UTPs are ideal for use as substrates in in vitro transcription reactions. These modified nucleotides can be utilized by a variety of RNA polymerases, including T7, T3, and SP6 RNA polymerases, as well as other RNA modifying enzymes. It is provided in one tube containing 25 μL (250 nmol). Biotinylated RNA can be used in place of radioactively labeled RNA in many applications, including cRNA amplification and labeling for microarray target preparation. Detection is then carried out via one of a variety of streptavidin-based methods. This Bio-UTP is confirmed free of non-specific endonuclease activity, exonuclease activity, and RNase activity and is functionally tested using the MessageAmp™ II aRNA Amplification Kit (SKU# AM1751).

Bio-11-UTP (75 mM) (Invitrogen™)

Ambion® Biotinylated UTPs are ideal for use as substrates in in vitro transcription reactions. These modified nucleotides can be utilized by a variety of RNA polymerases, including T7, T3, and SP6 RNA polymerases, as well as other RNA modifying enzymes. It is provided in one tube containing 30 µL (2,250 nmol). Biotinylated RNA can be used in place of radioactively labeled RNA in many applications, including cRNA amplification and labeling for microarray target preparation. Detection is then carried out via one of a variety of streptavidin-based methods. This Bio-UTP is confirmed free of non-specific endonuclease activity, exonuclease activity, and RNase activity and is functionally tested using the MessageAmp™ II aRNA Amplification Kit (SKU# AM1751).

Biotin-14-dATP (Invitrogen™)

Biotin-14-dATP is a patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP (1). Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase (2). The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation.

Performance and Quality Testing: Purity is evaluated by reverse-phase HPLC analysis.

Biotin-14-dCTP (Invitrogen™)

Biotin-14-dCTP is used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling. Advantages of using biotinylated DNA probes include:

• Convenience and safety—probe preparation generates no radioactive waste
• Stability—probes remain active longer than isotopically labeled probes
• Versatility—biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding

About Biotin-14-dCTP
Biotin-14-dCTP is a patented dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, and dTTP, labeling DNA with terminal deoxynucleotide transferase, or random primer synthesis. The amount of material provided is sufficient for labeling up to 50 µg of DNA.

Performance and quality testing
Purity is evaluated by reverse-phase HPLC.

Bio-16-UTP (10 mM) (Invitrogen™)

Ambion® Biotinylated UTPs are ideal for use as substrates in in vitro transcription reactions. These modified nucleotides can be utilized by a variety of RNA polymerases, including T7, T3, and SP6 RNA polymerases, as well as other RNA modifying enzymes. It is provided in one tube containing 25 μL (250 nmol). Biotinylated RNA can be used in place of radioactively labeled RNA in many applications, including cRNA amplification and labeling for microarray target preparation. Detection is then carried out via one of a variety of streptavidin-based methods. This Bio-UTP is confirmed free of non-specific endonuclease activity, exonuclease activity, and RNase activity and is functionally tested using the MessageAmp™ II aRNA Amplification Kit (SKU# AM1751).

Biotin-14-CTP (Invitrogen™)

Biotin-14-CTP is a CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker. Biotin-14-CTP can be efficiently incorporated into RNA transcripts using T7, SP6, or T3 RNA polymerase and the appropriate template in the presence of ATP, GTP, UTP, and Biotin-14-CTP (1). The biotinylated RNA can be used as a probe in membrane-based or in situ hybridizations (2). The amount of material provided is sufficient for up to 20 transcription reactions (1 µg template).

Performance and Quality Testing: Purity is evaluated by reverse-phase HPLC.