Shop All dNTPs

dNTPs for cDNA Probe Synthesis (10 mM) (Invitrogen™)

Ambion® dNTPs for cDNA Probe Synthesis (10 mM) are the same high-quality deoxynucleotide triphosphate solutions provided in the RETROscript® First Strand cDNA Synthesis Kit for RT-PCR (SKU# AM1710). They are subject to rigorous nuclease testing as well as a functional assay to ensure high levels of incorporation. Supplied as a set of 10 mM solutions in four individual tubes containing 75 μL each.

dATP (10 mM) (Invitrogen™)

Invitrogen dATP is prepared to a final concentration of 10 mM in 0.9 mM Tris-HCl. 10 mM dATP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Invitrogen dNTP manufacturing meets the highest industry standards:
• Manufactured under ISO 9001
• Manufactured in Class D clean rooms

Features include:
• Chemically synthesized
• pH 7.5
• >99% purity confirmed by HPLC
• Stable for 2 years at –20°C
• Free from qPCR, PCR, reverse transcription inhibitors
• Free of DNases and RNases
• Free of human and E. coli DNA

Applications:
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA synthesis
• High-fidelity and long-range PCR
• Isothermal amplification
• DNA labeling
• Cloning
• Sanger and next-generation sequencing (NGS) sequencing

dNTPs in a different concentration or format ›

dNTP Mix (2 mM each) (Thermo Scientific™)

Thermo Scientific dNTP Mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 2 mM. The nucleotides have greater than 99% purity, are free of nuclease activities, human and E. coli DNA. Mixes offer the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. They are designed for many different molecular biology applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA
• Highly stable
• Stable for years at -20°C
• Stable after multiple freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C; 3 minutes at 72°C)

Application tested in:
• Long range PCR (40 kb)
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR
• High fidelity PCR

Applications
• For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

dNTP Mix (10 mM each) (Thermo Scientific™)

Thermo Scientific dNTP Mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM. The nucleotides have greater than 99% purity, are free of nuclease activities, human and E. coli DNA. Mixes offer the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. They are designed for many different molecular biology applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA
• Highly stable
• Stable for years at -20°C
• Stable after multiple freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C; 3 minutes at 72°C)

Application tested in:
• Long range PCR (40 kb)
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR
• High fidelity PCR

Applications
• For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

dNTP Mix (2.5 mM) (Invitrogen™)

The same high-quality deoxynucleotide triphosphate solutions provided in the Ambion® RETROscript® First Strand cDNA Synthesis Kit for RT-PCR (SKU# AM1710). They are subject to rigorous nuclease testing as well as a functional assay to ensure high levels of incorporation. Supplied in one tube containing 0.3 mL, premixed at a concentration of 2.5 mM each.

dCTP (10 mM) (Invitrogen™)

Invitrogen dCTP is prepared to a final concentration of 10 mM in 0.9 mM Tris-HCl. 10 mM dCTP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Invitrogen dNTP manufacturing meets the highest industry standards:
• Manufactured under ISO 9001
• Manufactured in Class D clean rooms

Features include:
• Chemically synthesized
• pH 7.5
• >99% purity confirmed by HPLC
• Stable for 2 years at –20°C
• Free from qPCR, PCR, reverse transcription inhibitors
• Free of DNases and RNases
• Free of human and E. coli DNA

Applications:
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA synthesis
• High-fidelity and long-range PCR
• Isothermal amplification
• DNA labeling
• Cloning
• Sanger and next-generation sequencing (NGS) sequencing

dNTPs in a different concentration or format ›

dATP Solution (100 mM) (Thermo Scientific™)

Thermo Scientific dATP (2'-deoxyadenosine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. The nucleotide has greater than 99% purity, is free of nuclease activities, human and E. coli DNA. It is designed for many different molecular biology applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA
• Highly stable

Application tested in:
• Long range PCR (40 kb)
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR
• High fidelity PCR

Applications

• For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

dNTP Mix (25 mM each) (Thermo Scientific™)

Thermo Scientific dNTP Mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 25 mM. The nucleotides have greater than 99% purity, are free of nuclease activities, human and E. coli DNA. Mixes offer the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. They are designed for many different molecular biology applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA
• Highly stable
• Stable for years at -20°C
• Stable after multiple freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C; 3 minutes at 72°C)

Application tested in:
• Long range PCR (40 kb)
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR
• High fidelity PCR

Applications
• For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

dUTP Solution (100 mM) (Thermo Scientific™)

Thermo Scientific dUTP (2'-deoxyuridine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. The nucleotide has greater than 99% purity, is free of nuclease activities, human and E. coli DNA. It is designed for many different molecular biology applications.

Highlights

• Greater than 99% purity—confirmed by HPLC
• Free of human and E. coli DNA
• Highly stable

Application tested in:
• Long range PCR (40 kb)
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR
• High fidelity PCR
• Applications
• For use in all molecular biology applications, including PCR, real-time PCR and RT-PCR with Taq DNA polymerase, cDNA synthesis, and primer extension.

Note: dUTP is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.

dGTP (10 mM) (Invitrogen™)

Invitrogen dGTP is prepared to a final concentration of 10 mM in 0.9 mM Tris-HCl. 10 mM dGTP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Invitrogen dNTP manufacturing meets the highest industry standards:
• Manufactured under ISO 9001
• Manufactured in Class D clean rooms

Features include:
• Chemically synthesized
• pH 7.5
• >99% purity confirmed by HPLC
• Stable for 2 years at –20°C
• Free from qPCR, PCR, reverse transcription inhibitors
• Free of DNases and RNases
• Free of human and E. coli DNA

Applications:
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA synthesis
• High-fidelity and long-range PCR
• Isothermal amplification
• DNA labeling
• Cloning
• Sanger and next-generation sequencing (NGS) sequencing

dNTPs in a different concentration or format ›

dITP Solution (100 mM) (Thermo Scientific™)

Thermo Scientific dITP (2'-deoxyinosine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. The nucleotide has greater than 99% purity, is free of nuclease activities, human and E. coli DNA. It is designed for many different molecular biology applications.

Applications

• For use in all molecular biology applications, including PCR and RT-PCR with Taq DNA polymerase, cDNA synthesis, SNP genotyping, and primer extension.

Note
• dITP is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.

dTTP (10 mM) (Invitrogen™)

Invitrogen dTTP is prepared to a final concentration of 10 mM in 0.9 mM Tris-HCl. 10 mM dTTP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Invitrogen dNTP manufacturing meets the highest industry standards:
• Manufactured under ISO 9001
• Manufactured in Class D clean rooms

Features include:
• Chemically synthesized
• pH 7.5
• >99% purity confirmed by HPLC
• Stable for 2 years at –20°C
• Free from qPCR, PCR, reverse transcription inhibitors
• Free of DNases and RNases
• Free of human and E. coli DNA

Applications:
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA synthesis
• High-fidelity and long-range PCR
• Isothermal amplification
• DNA labeling
• Cloning
• Sanger and next-generation sequencing (NGS) sequencing

dNTPs in a different concentration or format ›

dGTP (100 mM) (Invitrogen™)

Invitrogen dGTP is prepared to a final concentration of 100 mM in highly purified water. 100 mM dGTP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Invitrogen dNTP manufacturing meets the highest industry standards:
• Manufactured under ISO 9001
• Manufactured in Class D clean rooms

Features include:
• Chemically synthesized
• pH 7.5
• >99% purity confirmed by HPLC
• Stable for 2 years at –20°C
• Free from qPCR, PCR, reverse transcription inhibitors
• Free of DNases and RNases
• Free of human and E. coli DNA

Applications:
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA synthesis
• High-fidelity and long-range PCR
• Isothermal amplification
• DNA labeling
• Cloning
• Sanger and next-generation sequencing (NGS) sequencing

dNTPs in a different concentration or format ›

GeneAmp™ dNTP Blend (2.5 mM ea) (Applied Biosystems™)

GeneAmp® dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. Each vial consists of 1 mL of 2.5 mM each of dATP, dCTP, dGTP, and dTTP, yielding a 10 mM blend of dNTPs. Key advantages of GeneAmp® dNTPs:

• dNTPs are of high purity, making them ideal for use in PCR reactions or other applications
• Guaranteed for PCR performance when used with GeneAmp® PCR core reagents and GeneAmp® instrument systems
• Premixed dNTP blends are available in various concentrations, offering ease of use

dNTP/dUTP Mix (Thermo Scientific™)

Thermo Scientific dNTP/dUTP Mix is a solution containing dATP, dCTP and dGTP, each at a final concentration of 2 mM and dUTP of 4 mM. All nucleotides have greater than 99% purity, are free of nuclease activities, human and E. coli DNA. The mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. It is designed for direct use in PCR with Taq DNA Polymerase, reverse transcription, and other applications.

Highlights

Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA

Highly stable
• Stable for a minimum 100 freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 min at 72°C)

Application tested in:
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR

Applications

• For use in all molecular biology applications, including PCR, real-time PCR & RT-PCR with Taq DNA Polymerase, cDNA synthesis, MDA, and DNA sequencing.

Note
• dNTP/dUTP Mix is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.