Shop All BrdU, EdU & EU Reagents

BrdU (5-Bromo-2´-Deoxyuridine) (Invitrogen™)

Cells can naturally incorporate the thymidine analog BrdU into their cells during cell division, making this nucleoside analog an excellent marker of by cell cycle and proliferation. Analysis of incorporated BrdU can be with an anti-BrdU antibody.

EU (5-Ethynyl Uridine) (Invitrogen™)

Detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide, ethynyl uridine (EU, E10345) or in kit form with Click-iT Nascent RNA capture kit, C-10365.
Both approaches use powerful click chemistry detection, a simple and robust two-step technique. In step one, the alkyne-containing biomolecule is fed to cells or animals and actively incorporated into RNA. Detection utilizes the chemoselective ligation or “click" reaction between an azide and an alkyne where the modified protein is detected with a corresponding azide-containing dye or hapten using the Click-iT® Cell Reaction Buffer Kit. Cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis (HCS).
By using the Click-iT Nascent RNA capture kit, the nascent pool of RNA can be isolated for transcriptome analysis, including RNAseq, ΔCT, RNA turnover, Nuclear run and run off, array analysis and more. Please see this link for more details on the Click-iT Nascent RNA capture kit: Click here.

BrdU Labeling Reagent

Application: Labeling with BrdU in vivo or in cultured cells

EdU (5-ethynyl-2’-deoxyuridine) (Invitrogen™)

EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows for more reproducible results, a simpler and quicker protocol and measurements which can easily be multiplexed with relevant antibody based targets including phospho-histone H3, Ki-67, and cyclin B1 as well as dual-pulse experiments with BrdU by flow cytometry, fluorescence microscopy, microplate (HTS) or high-throughput imaging (HCS).

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

BrUPT (5-Bromouridine 5´-Triphosphate), 10 mM in TE buffer (Invitrogen™)

BrUTP is an excellent substrate for RNA polymerase and has been used to monitor nucleolar transcription in situ. BrUTP can be detected with anti-BrdU antibodies.

BrdUTP (5-Bromo-2´-Deoxyuridine 5´-Triphosphate), 10 mM in TE buffer (Invitrogen™)

BrdUTP can be used in conjunction with tetrminal deoxynucleotidyl transferase (TdT) to label apoptotic cells in the TUNEL assay. BrdUTP can be detected with anti-BrdU antibodies.