Shop All Conjugated Biomolecules

Lipopolysaccharides From Escherichia coli Serotype 055:B5, Alexa Fluor™ 568 Conjugate Invitrogen™

This bright, orange-red—fluorescent Alexa Fluor 568 conjugate can be used to follow lipopolysaccharide binding, transport and cell-internalization processes.

CaptureSelect™ Biotin Anti-Prothrombin Conjugate Thermo Scientific™

CaptureSelect™ Biotin Anti-Prothrombin Conjugate consists of a 13 kDa camelid antibody fragment (affinity ligand) that specifically binds with high affinity and selectivity to human prothrombin (coagulation factor II). The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces. This conjugate allows you to detect, quantitate, and characterize native and recombinant human prothrombin.

Applications for CaptureSelect Biotin Anti-Prothrombin Conjugate include ELISA, immunoprecipitation with streptavidin-coupled Dynabeads™, MSIA Streptavidin D.A.R.T.’S, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

α-Bungarotoxin, biotin-XX conjugate Invitrogen™

α-Bungarotoxin, a 74-amino acid peptide extracted from Bungarus multicinctus venom, binds with high affinity to the α-subunit of the nicotinic acetylcholine receptor (AChR) of neuromuscular junctions. The biotin-XX α-bungarotoxin can be used in conjunction with labeled streptavidin or avidin to visualize this receptor. Labeled -bungarotoxin conjugates can be used to facilitate identification of nicotinic AChRs and to localize neuromuscular junctions.

Lipopolysaccharides From Escherichia coli Serotype 055:B5, Alexa Fluor™ 594 Conjugate Invitrogen™

This bright, red-fluorescent Alexa Fluor 594 conjugate can be used to follow lipopolysaccharide binding, transport and cell-internalization processes.

Fluorescein-TRAP220/DRIP-1 peptide

LanthaScreen® fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen® TR-FRET Nuclear Receptor Coregulator Assays. Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.

How they work
Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen® panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide. In the LanthaScreen® TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide (Figure 1). The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio (Figure 2).

Contents and Storage:

LanthaScreen® fluorescent nuclear receptor coregulator peptides are supplied as 100 µM solutions in peptide-appropriate buffers. Store peptides at -20°C.

CaptureSelect™ Biotin CH1-XL Conjugate Thermo Scientific™

CaptureSelect Biotin CH1-XL Conjugate consists of a 14-kDa camelid antibody fragment (affinity ligand) that specifically binds to the CH1 domain of all four human IgG subclasses. The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

CaptureSelect Biotin CH1-XL Conjugate is an improved version of CaptureSelect Biotin Anti-IgG-CH1 for applications that require target elution at a milder pH. The specificity is the same, but it features a wider elution range between pH 3.0 and 4.5.

This conjugate allows you to detect, quantitate, and characterize all human IgG antibodies (subclasses 1 to 4) and their Fab fragments, independent of light chain type, and total IgG in human plasma and/or serum samples.

Applications for CaptureSelect Biotin CH1-XL Conjugate include ELISA, western blot, Gyrolab™-based immunoassays, immunoprecipitation with streptavidin-coupled Dynabeads, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Additional information and other CaptureSelect biotin ligands ›

Wheat Germ Agglutinin, Oregon Green™ 488 Conjugate Invitrogen™

Wheat germ agglutinin (WGA) is one of the most widely used lectins in cell biology. Our Oregon Green® 488 conjugate of WGA exhibits the bright, green fluorescence of the Oregon Green® 488 dye (excitation/emission maxima ~496/524 nm). Oregon Green® 488 WGA binds to sialic acid and N-acetylglucosaminyl residues.

View complete list of fluorescent dye-conjugated lectins ›

Fluorescein DHPE (N-(Fluorescein-5-Thiocarbamoyl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) Invitrogen™

The phospholipid, fluorescein DHPE is labeled on the head group with the green-fluorescent pH-sensitive fluorophore, fluorescein (excitation/emission maxima ~496/519 nm).

Concanavalin A, succinylated, Alexa Fluor™ 488 Conjugate Invitrogen™

This green-fluorescent Alexa Fluor 488 conjugate of succinylated concanavalin A (Con A) selectively binds to α-mannopyranosyl and α-glucopyranosyl residues. In acidic solutions (pH 4.5—5.6), Con A exists as a dimer with molecular weight of approximately 52,000 daltons; above pH 7, it is primarily a tetramer with a molecular weight of 104,000 daltons. Succinylation of Con A prevents tetramer formation, thus greatly reducing the lectin's agglutination activity but not its reactivity with glucose and mannose residues.

View complete list of fluorescent dye-conjugated lectins ›

CaptureSelect™ Biotin Anti-LC-kappa (Human) Conjugate Thermo Scientific™

CaptureSelect™ Biotin Anti-LC-kappa (Hu) Conjugate consists of a 13 kDa llama antibody fragment (affinity ligand) that specifically binds to the constant domain of human kappa light chains. The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect™ Biotin Anti-LC-kappa (Hu) Conjugate allows you to:

Detect, quantitate, and characterize all human antibody isotypes and subclasses (for example, IgG1 to 4, IgM, IgA, IgD, and IgE), and Fab fragments thereof, that contain a kappa light chain; as well as recombinant human Fab fragments and/or Fab and Fab2 fragments obtained after papain or pepsin digestion
Avoid cross-binding with human antibodies that possess a lambda light chain or IgG from bovine sources such as FCS
Screen antibody-antigen interactions—biotin anti-LC-kappa (Hu) forms a stable [low k(diss)] complex with, for example, human IgG antibodies through bivalent binding

Applications for CaptureSelect™ Biotin Anti-LC-kappa (Hu) Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Lipopolysaccharides from Salmonella Minnesota, Alexa Fluor™ 488 conjugate Invitrogen™

This bright, green-fluorescent Alexa Fluor 488 conjugate can be used to follow lipopolysaccharide binding, transport and cell-internalization processes.

FL - RIP140L6 Peptide, 100µM

LanthaScreen® fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen® TR-FRET Nuclear Receptor Coregulator Assays. Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.

How they work
Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen® panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide. In the LanthaScreen® TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide (Figure 1). The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio (Figure 2).

Contents and Storage:

LanthaScreen® fluorescent nuclear receptor coregulator peptides are supplied as 100 µM solutions in peptide-appropriate buffers. Store peptides at -20°C.

Wheat Germ Agglutinin Sampler Kit - Four Fluorescent Conjugates, 1 mg each Invitrogen™

Wheat germ agglutinin (WGA) is one of the most widely used lectins in cell biology. The Wheat Germ Agglutinin Sampler Kit contains 1 mg each of the following WGA fluorescent conjugates, blue-fluorescent Alexa Fluor® 350 WGA, green-fluorescent Oregon Green® 488 WGA, orange-fluorescent tetramethylrhodamine WGA and red-fluorescent Texas Red® WGA. WGA binds to sialic acid and N-acetylglucosaminyl residues.

View complete list of fluorescent dye-conjugated lectins ›

Dextran, Texas Red™, 40,000 MW, Neutral Invitrogen™

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Dextran Specifications:

Label (Ex/Em): Texas Red® (595/615)
Size: 40,000 MW
Charge: Zwitterionic
Fixable: Nonfixable

High Manufacturing Standards of Molecular Probes® Dextrans
We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

In most cases, Molecular Probes® fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

A Wide Selection of Substituents and Molecular Weights
Molecular Probes® dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor® dyes (Molecular Probes dextran conjugates–Table 14.4) and are available in these nominal molecular weights (MW): 3,000; 10,000; 40,000; 70,000; 500,000; and 2,000,000 daltons.

Dextran Net Charge and Fixability
We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor® 488 dextrans results in the final product being neutral, anionic, or cationic. The Alexa Fluor®, Cascade Blue®, lucifer yellow, fluorescein, and Oregon Green® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red® dyes are essentially neutral. To produce more highly anionic dextrans, we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers; these products are designated "polyanionic" dextrans.

Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. For these applications, we offer "lysine-fixable" versions of most of our dextran conjugates of fluorophores or biotin. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques. We have also shown that all of our 10,000 MW Alexa Fluor® dextran conjugates can be fixed with aldehyde-based fixatives.

Key Applications Using Labeled Dextrans
There are a multitude of citations describing the use of labeled dextrans. Some of the most common uses include:

Neuronal tracing (anterograde and retrograde) in live cells
Cell lineage tracing in live cells
Neuroanatomical tracing
Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
Investigating vascular permeability and blood–brain barrier integrity
Tracking endocytosis
Monitoring acidification (some dextran–dye conjugates are pH-sensitive)
Studying the hydrodynamic properties of the cytoplasmic matrix

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Lectin PNA From Arachis hypogaea (peanut), Alexa Fluor™ 647 Conjugate Invitrogen™

Lectin PNA is specific for terminal β-galactose. It will agglutinate human erythrocytes, but only after neuraminidase treatment.

View complete list of fluorescent dye-conjugated lectins ›

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