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Pierce™ Horseradish Peroxidase, Biotinylated Thermo Scientific™

Thermo Scientific Pierce Biotinylated Proteins include biotin-labeled proteins (BSA), enzymes (HRP, AP) and fluorophores (FITC) for use as controls or signal amplification in IHC via avidin-biotin complex (ABC) techniques.

Biotinylated HRP is most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method.

Biotinylated enzymes—biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and beta-galactosidase (B-bGal) for use in avidin-biotin complex (ABC) staining

Biotin, also known as vitamin H, is a small molecule (MW 244.3) that is present in tiny amounts in all living cells and is critical for a number of biological processes. The valeric acid side chain of the biotin molecule can be derivatized in order to incorporate various reactive groups that are used to attach biotin to other molecules. In the context of immunohistochemistry (IHC), biotin is conjugated to antibodies or to the enzyme reporters used to detect target antigens.

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Pierce™ Bovine Serum Albumin, Biotinylated
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Pierce™ Biotin-Fluorescein Conjugate
Pierce™ Biotin

Gelatin From Pig Skin, Oregon Green™ 488 Conjugate Invitrogen™

Molecular Probes® fluorescent conjugates of gelatin are designed for researchers studying not only collagen-binding proteins and collagen metabolism, but also for the study of gelatinases and collagenases (metalloproteins that digest collagen and gelatin). We offer two fluorescent conjugates of gelatin—one in which gelatin is coupled to fluorescein (G13187) and the other in which gelatin is coupled to our Oregon Green® 488 dye (G13186).

Gelatin Conjugate Specifications:
• Label (Ex/Em): Oregon Green® 488 (~499/519 nm)
• Degree of labeling is listed on the product label (based on molecular weight of 100,000)


Find More Probes for Following Receptor Binding and Cell Adhesion
Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 and Probes for Cell Adhesion, Chemotaxis, Multidrug Resistance and Glutathione—Section 15.6 in the Molecular Probes® Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Transferrin From Human Serum, Fluorescein Conjugate Invitrogen™

Transferrin is a monomeric serum glycoprotein (~80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways.

These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.

Transferrin Specifications:

Label (Ex/Em): Fluorescein (495/518)

Amount: 15 mg solid (contains 5 mg of transferrin conjugate)

Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 488 Streptavidin (Alexa Fluor™ 488 EGF complex) Invitrogen™

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor® 488 streptavidin (~495/519 nm)
• Number of fluorophore molecules on each EGF molecule: ~2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

Gelatin From Pig Skin, Fluorescein Conjugate Invitrogen™

Molecular Probes® fluorescent conjugates of gelatin are designed for researchers studying not only collagen-binding proteins and collagen metabolism, but also for the study of gelatinases and collagenases (metalloproteins that digest collagen and gelatin). We offer two fluorescent conjugates of gelatin—one in which gelatin is coupled to fluorescein (G13187) and the other in which gelatin is coupled to our Oregon Green® 488 dye (G13186).

Gelatin Conjugate Specifications:
• Label (Ex/Em): Fluorescein (~499/519 nm)
• Degree of labeling is listed on the product label (based on molecular weight of 100,000)


Find More Probes for Following Receptor Binding and Cell Adhesion
Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 and Probes for Cell Adhesion, Chemotaxis, Multidrug Resistance and Glutathione—Section 15.6 in the Molecular Probes® Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Vybrant™ Alexa Fluor™ 488 Lipid Raft Labeling Kit Invitrogen™

Vybrant Lipid Raft Labeling Kits are designed to provide convenient, reliable and extremely bright fluorescent labeling of lipid rafts in live cells. Kits include cholera toxin subunit B (CT-B) labeled with either the green-fluorescent Alexa Fluor 488, the orange-fluorescent Alexa Fluor 555 or the red-fluorescent Alexa Fluor 594 dye, as well as an antibody that specifically recognizes CT-B. A 10X solution of phosphate-buffered saline (10X PBS), pH 7.2, and detailed instructions are also included in the kits.

Albumin from Bovine Serum (BSA), FITC conjugate Invitrogen™

This albumin from bovine serum (BSA), fluorescein conjugate is suited for applications in which well-defined molecular weight tracers are needed. These applications include studying the rate of endocytosis and exocytosis and following intracellular protein processing.

Transferrin From Human Serum, Alexa Fluor™ 555 Conjugate Invitrogen™

Transferrin is a monomeric serum glycoprotein (~80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways.

These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.

Transferrin Specifications:

Label (Ex/Em): Alexa Fluor® 555 (555/565)

Amount: 15 mg solid (contains 5 mg of transferrin conjugate)

Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Transferrin From Human Serum, Tetramethylrhodamine Conjugate Invitrogen™

Transferrin is a monomeric serum glycoprotein (~80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways.

These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.

Transferrin Specifications:

Label (Ex/Em): Tetramethylrhodamine (555/580)

Amount: 15 mg solid (contains 5 mg of transferrin conjugate)

Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Transferrin From Human Serum, Biotin-XX Conjugate Invitrogen™

Transferrin is a monomeric serum glycoprotein (~80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways.

These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.

Transferrin Specifications:

Label (Ex/Em): Biotin-XX (contains a 14 atom spacer separating the biotin from the protein)

Amount: 15 mg solid (contains 5 mg of transferrin conjugate)

Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Actin, from rabbit muscle, Alexa Fluor™ 488 conjugate, in solution Invitrogen™

The green-fluorescent Alexa Fluor® 488 actin conjugate is an important tool for investigating cytoskeleton dynamics in-vivo. This fluorescent conjugate is prepared by labeling the amine residues of polymerized F-actin. After labeling, the conjugates are subjected to depolymerization and subsequent polymerization to ensure that the actin conjugates are able to assemble properly. The labeled actin that polymerizes is then separated from remaining monomeric actin by centrifugation, depolymerized and packaged in monomeric form.

Heparin, Fluorescein Conjugate Invitrogen™

The green-fluorescent fluorescein heparin conjugate should be a useful tool for studying binding of this mucopolysaccharide in cells and tissue.

Epidermal Growth Factor, Biotin-XX Conjugate (biotin EGF) Invitrogen™

This purified epidermal growth factor (EGF) contains a single biotin-XX molecule on the N-terminal amino acid. Biotin-XX EGF contains a long spacer arm that enhances the probe’s affinity for the EGF receptor and facilitates binding of a label such as a dye–streptavidin molecule, a Qdot® nanocrystals–streptavidin molecule, or an enzyme-conjugated streptavidin. Labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more.

Epidermal Growth Factor Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Number of biotin-XX molecules on each EGF molecule: 1


Find Fluorescent EGF Conjugates
In addition to biotinylated EGF, we offer biotinylated EGF precomplexed to selected fluorescent streptavidin labels and EGF directly labeled with some fluorescent dyes. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Vybrant™ Alexa Fluor™ 555 Lipid Raft Labeling Kit Invitrogen™

Vybrant Lipid Raft Labeling Kits are designed to provide convenient, reliable and extremely bright fluorescent labeling of lipid rafts in live cells. Kits include cholera toxin subunit B (CT-B) labeled with either the green-fluorescent Alexa Fluor 488, the orange-fluorescent Alexa Fluor 555 or the red-fluorescent Alexa Fluor 594 dye, as well as an antibody that specifically recognizes CT-B. A 10X solution of phosphate-buffered saline (10X PBS), pH 7.2, and detailed instructions are also included in the kits.

Albumin from Bovine Serum (BSA), Alexa Fluor™ 647 conjugate Invitrogen™

This albumin from bovine serum (BSA), Alexa Fluor® 647 conjugate is suited for applications in which well-defined molecular weight tracers are needed. These applications include studying the rate of endocytosis and exocytosis and following intracellular protein processing.
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