Shop All Biochemical, Drug & Antibiotic Conjugates
The green-fluorescent fluorescein methotrexate (absorption⁄emission maxima ~496⁄516 nm) can be used to probe dihydrofolate reductase (DHFR) expression by fluorescence microscopy or flow cytometry.
BOCILLIN™ FL Penicillin, Sodium Salt (Invitrogen™)
Penicillin-binding proteins, present on the cytoplasmic membranes of eubacteria, can be detected with our BOCILLIN FL penicillin and BOCILLIN 650/665 penicillin (B-13234). BOCILLIN FL penicillin, synthesized from penicillin V and BODIPY FL dye (which is spectrally similar to fluorescein), has been used to determine the penicillin-binding protein profiles of a number of different bacteria types.
Thapsigargin is a naturally occurring sesquiterpene lactone isolated from the umbelliferous plant Thapsia garganica. This tumor promoter releases Ca2+ from intracellular stores by specifically inhibiting the endoplasmic reticulum Ca2+-ATPase; it does not directly affect plasma membrane Ca2+-ATPases, Ins 1,4,5-P3 production or protein kinase C activity. Thapsigargin is also available as a special packaging, T7459.
BODIPY™ FL Prazosin (Invitrogen™)
Prazosin is a high-affinity antagonist for the a1-adrenergic receptor. The green-fluorescent BODIPY® FL prazosin derivative can be used to localize and detect the a1-adrenergic receptor.
BODIPY™ FL Ouabain (Invitrogen™)
Ouabain is a member of a class of glycosylated steroids collectively known as cardiac glycosides due to their therapeutic efficacy in the treatment of congestive heart failure. Ouabain inhibits the ion transport activity of the plasma membrane Na+/K+-ATPase via binding to its catalytic α-subunit. This BODIPY FL ouabain derivative offers some valuable properties. Because the BODIPY FL fluorophore is nonpolar and relatively small, labeled ligands typically have superior receptor-binding properties over fluorescein- and NBD-labeled derivatives.
Vancomycin, BODIPY™ FL Conjugate (BODIPY™ FL Vancomycin) (Invitrogen™)
BODIPY® FL vancomycin, which contains a single BODIPY® dye per vancoymycin molecule, is a green-fluorescent analog of this important antibiotic, which is active against gram-positive bacteria, including entereococci. Although its biological activity has not been reported, it may be useful for detecting vancomycin binding sites and transport, for fluorescence polarization assays, and for the study and detection of vancomycin-resistant enterococci.
Dexamethasone Fluorescein (Invitrogen™)
The synthetic steroid hormone dexamethasone binds to the glucocorticoid receptor, producing a steroid-receptor complex that then localizes in the nucleus and regulates gene transcription. The green fluorescent analog, fluorescein dexamethasone should be useful for studying the mechanism of glucocorticoid receptor activation, although at a slower rate than unmodified dexamethasone.