Shop All Lipid Conjugates

NBD-PE (N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) (Invitrogen™)

The phospholipid, NBD-PE is labeled on the head group with the environment sensitive fluorophore, NBD (excitation/emission maxima ~463/536 nm).

Biotin-X DHPE (N-((6-(Biotinoyl)amino)hexanoyl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) (Invitrogen™)

The biotinylated phospholipid, biotin-X DHPE can be used to couple avidin or streptavidin to cell membranes, liposomes and lipoid monolayers.

D-Myo-Inositol 1,4,5-Triphosphate, P4(5)-(1-(2-Nitrophenyl)ethyl) Ester, tris(triethylammonium) Salt (NPE-Caged Ins 1,4,5-P3) (Invitrogen™)

NPE-caged inositol 1,4,5-triphosphate is biologically inactive before photolysis. Flash photolysis of the caging group with UV illumination at 355 nm results in a rapid and highly localized release of free INS 1,4,5-P3 at the site of illumination.

β-BODIPY™ 500/510 C12-HPC (2-(4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoyl)-1-Hexadecanoyl-sn-Glycero-3-Phosphocholine) (Invitrogen™)

The intensely fluorescent BODIPY® (4,4-difluoro-3a,4a-diaza-s-indacene) fluorophore is intrinsically lipophilic, unlikemost other long-wavelength dyes. Consequently, probes incorporating this fluorophore are more likely to mimic the properties of natural lipids. BODIPY® fatty acid analogs like BODIPY® 500/510 dodecanoic acid (D-3823) and BODIPY FL dodecanoic acid (D-3822) undergo native-like transport and metabolism in cells. They are therefore effective tracers of lipid trafficking, as well as being useful general-purpose membrane probes.

Texas Red™ 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt (Texas Red™ DHPE) (Invitrogen™)

The phospholipid, Texas Red® 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Texas Red® DHPE) is labeled on the head group with the bright, red-fluorescent Texas Red® dye (excitation/emission maxima ~595/615 nm).

BODIPY™ 505/515 (4,4-Difluoro-1,3,5,7-Tetramethyl-4-Bora-3a,4a-Diaza-s-Indacene) (Invitrogen™)

With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY® 505/515 can be used as a stain for netural lipds and as a tracer for oil and other nonpolar lipids.

BODIPY™ FL DHPE (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionyl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) (Invitrogen™)

BODIPY® DHPE has the green-fluorescent dye attached to the head group of the phospholipid. BODIPY® FL can form excimers at high concentrations and unlike fluorophores like pyrene, DPH and NBD, BODIPY® FL is relatively environment insensitive and is fluorescent in both aqueous and lipid environments.

BODIPY™ 500/510 C4, C9 (5-Butyl-4,4-Difluoro-4-Bora-3a,4a-Diaza-s-Indacene-3-Nonanoic Acid) (Invitrogen™)

The green-fluorescent fatty acid BODIPY® 500/510 C4, C9 has the fluorophore linked within the fatty acid chain. BODIPY® fatty acids are readily metabolized by living cells to phospholipids, di- and triacylglycerols, cholesteryl esters and other natural lipids.

NBD C6-Ceramide Complexed to BSA (Invitrogen™)

NBD C6-ceramide can be used to study sphingolipid transport and metabolism mechanisms. It is also used as a selective stain for the Golgi apparatus in live and fixed cells. Complexing fluorescent lipids with bovine serum albuin (BSA) facilitates cell labeling by eliminating the need for organic solvents to dissolve the lipophilic probe - the BSA-complexed probe can be directly dissolved in water. The environment sensitive NBD dye is weakly fluorescent in water but increases in aprotic solvents and other nonpolar environments with excitation/emission maxima ~466/536 nm.

PED6 (N-((6-(2,4-Dinitrophenyl)amino)hexanoyl)-2-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)-1-Hexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) (Invitrogen™)

Molecular Probes® phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2. Fluorescently labeled versions of the cleavage product generated from these substrates are also available for use as standards for evaluating percentage substrate conversion in enzymatic reactions.

Fluorogenic Phospholipase A Substrates Specifications:
• Label (Ex/Em): PED6
• Specificity: PLA2 (this substrate is cleaved by PLA1, but this reaction does not separate the fluorophore from the quencher)
• Cleavage product: D3834
• Lyophilized product can be dissolved in DMSO for use
• Fluorescence is measured either in microplate assays or using flow cytometry


Find More Probes for Lipid Metabolism and Fatty Acid Analogs
Review Fatty Acid Analogs and Phospholipids—Section 13.2 and Probes for Lipid Metabolism and Signaling—Section 17.4 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

β-Py-C10-HPC (1-Hexadecanoyl-2-(1-Pyrenedecanoyl)-sn-Glycero-3-Phosphocholine) (Invitrogen™)

This phospholipid analog of phosphocholine, ß-py-C10-HPC is labeled with pyrene on the sn-2 acyl chain. Pyrene forms excimers at high concentrations, excimer emission ~470nm. Pyrene is environment sensitive, essentially nonfluorescent in water.

BODIPY™ FL C5 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoic Acid) (Invitrogen™)

The green fluorescent fatty acid, BODIPY® FL C5 can be used as a synthetic precursor to a wide variety of fluorescent phospholipids. Unlike fluorophores like pyrene, DPH and NBD, BODIPY® dyes are relatively environment insensitive and is fluorescent in both aqueous and lipid environments.

NBD C6-Ceramide (6-((N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)amino)hexanoyl)Sphingosine) (Invitrogen™)

NBD C6-ceramide can be used to study sphingolipid transport and metabolism mechanisms. It is also used as a selective stain for the Golgi apparatus in live and fixed cells. The environment sensitive NBD dye is weakly fluorescent in water but increases in aprotic solvents and other nonpolar environments with excitation/emission maxima ~466/536 nm.

BODIPY™ TR Ceramide complexed to BSA (Invitrogen™)

The red-fluorescent BODIPY® TR C5 ceramide complexed to BSA can be used in sphingolipid transport and metabolism mechanisms. Complexing fluorescent lipids with bovine serum albuin (BSA) facilitates cell labeling by eliminating the need for organic solvents to dissolve the lipophilic probe - the BSA-complexed probe can be directly dissolved in water.

BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) (Invitrogen™)

With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY® 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids.