Shop All Miscellaneous Conjugates

Alexa Fluor™ 594 Biocytin, Sodium Salt (Biocytin Alexa Fluor™ 594) (Invitrogen™)

The cell-impermeant, fixable, polar tracer Alexa Fluor® 594 biocytin combines the red-fluorescent Alexa Fluor® 594 fluorophore with biotin and an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. This water-soluble tracer can be introduced into cells by whole-cell patch clamping, iontophoresis, osmotic lysis of pinocytic vesicles or comparable methods. The biotin allows for detection and subsequent amplification with enzyme-modified streptavidins including horseradish (HRP) and alkaline phosphatase (AP) that can be used with TSA and ELF® technologies, respectively.

5(6)-CFDA (5-(and-6)-Carboxyfluorescein Diacetate), mixed isomers (Invitrogen™)

5(6)-CFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, this AM ester also yields carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells.

DiIC1(5) (1,1',3,3,3',3'-Hexamethylindodicarbocyanine Iodide) (Invitrogen™)

The cationic carbocyanine DiIC1(5) can be used as a slow-response potential-sensitive probe. This dye accumulates on hyperpolarized membranes and is translocated into the lipid bilyaer. Aggregation within the confined membrane interior usually results in decreased fluorescence and absorption shifts. This far red-fluorescent dye exhibits excitation/emission maxima of ~638/658 nm.

Calcein, AM, cell-permeant dye (Invitrogen™)

Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to green-fluorescent calcein, after acetoxymethyl ester hydrolysis by intracellular esterases. This dye is also available in our special packaging (C-3100) and resuspended in DMSO (C-3099). For a longer wavelength version of this dye check out our new CellTrace calcein red-orange AM (C-34851).

5-CFDA, AM (5-Carboxyfluorescein Diacetate, Acetoxymethyl Ester) (Invitrogen™)

5-CFDA, AM is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. The electronically neutral AM ester of CFDA can be loaded into cells at lower concentrations than CFDA. Upon hydrolysis by intracellular esterases, this AM ester also yields carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells.

Biocytin Alexa Fluor™ 488 (Alexa Fluor™ 488 Biocytin, Disodium Salt) (Invitrogen™)

The cell-impermeant, fixable, polar tracer Alexa Fluor® 488 biocytin combines the green-fluorescent Alexa Fluor® 488 fluorophore with biotin and an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. This water-soluble tracer can be introduced into cells by whole-cell patch clamping, iontophoresis, osmotic lysis of pinocytic vesicles or comparable methods. The biotin allows for detection and subsequent amplification with enzyme-modified streptavidins including horseradish (HRP) and alkaline phosphatase (AP) that can be used with TSA and ELF technologies, respectively.

Calcein, AM (Invitrogen™)

Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent calcein, after acetoxymethyl ester hydrolysis by intracellular esterases. This dye is also available in our special packaging (C-3100) and as 1 mg of the solid (C-1430). For a longer wavelength version of this dye, check out our new CellTrace calcein red-orange AM (Cat. no. C-34851).

Biocytin Hydrazide (Invitrogen™)

Biocytin hydrazide an be used as microinjectable anterograde and transneuronal tracers that is fixable with aldehyde-based fixatives. Additionally, the primary aliphatic amine of this molecule can be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be reduced to a stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3) to form new biotinylated probes. Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC (E2247).

Calcein, high purity (Invitrogen™)

Calcein is a polyanionic fluorescein derivative that exhibits fluorescence that is essentially independent of pH between 6.5 and 12. This versatile green fluorophore is cell impermeant and is commonly used for cell tracing and in studies of endocytosis and gap junctions.

Calcein Blue, AM (Invitrogen™)

Calcein Blue, AM is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Calcein blue AM is only weakly fluorescent (excitation/emission maxima ~322/435 nm). Upon cleavage of the AM esters by intracellular esterases, this tracer becomes relatively polar and is retained by cells for several hours. In addition, its fluorescence intensity increases and its fluorescence spectra shift to longer wavelengths, with excitation/emission maxima of ~360/449 nm.

Biocytin (ε-Biotinoyl-L-Lysine) (Invitrogen™)

Biocytin can be used as microinjectable anterograde and transneuronal tracers that is fixable with aldehyde-based fixatives. Additionally the primary amine on the lysine can be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3). Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC (E2247).

Biocytin TMR (5-(and-6)-Tetramethylrhodamine Biocytin) (Invitrogen™)

The water-soluble neuronal tracer 5-(and-6)-tetramethylrhodamine biocytin (biocytin TMR) can be detected in fixed tissues by using avidin or streptavidin probes. However, tetramethylrhodamine biocytin can also be observed directly in fixed or unfixed samples because the attached tetramethylrhodamine dye produces bright, orange-fluorescence (absorption/emission maxima ~554/581 nm).

5(6)-CFDA, SE; CFSE (5-(and-6)-Carboxyfluorescein Diacetate, Succinimidyl Ester), mixed isomers (Invitrogen™)

CFDA, SE is a fixable-cell-permeant, fluorescein-based tracer for very long-term cell labeling. The dye is also available in our convenient, easy-to-use Vybrant CFDA-SE Cell Tracer Kit (V12883).

Lucifer Yellow Biocytin, Potassium Salt (Biocytin Lucifer Yellow) (Invitrogen™)

The water-soluble neuronal tracer lucifer yellow biocytin can be detected in fixed tissues by using avidin or streptavidin probes. However, lucifer yellow biocytin can also be observed directly in fixed or unfixed samples because the attached lucifer yellow dye produces yellow fluorescence (absorption/emission maxima ~428/536 nm).

Fluorescein Diacetate (FDA) (Invitrogen™)

Fluorescein diacetate (FDA) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, this AM ester yields fluorescein.