Shop All Cellular Structure Probes

CellLight™ Golgi-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Golgi-RFP, BacMam 2.0, provides an easy way to label golgi with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to human golgi resident enzyme (N-acetylgalactosaminyltransferase). You can observe golgi-RFP behavior in live cells using fluorescent imaging and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Golgi-RFP, BacMam 2.0, is a fusion construct of human golgi resident enzyme (N-acetylgalactosaminyltransferase) and TagRFP, providing accurate and specific targeting to cellular golgi-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Histone 2B-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Histone 2B-GFP, BacMam 2.0, provides an easy way to label histone 2b with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to histone 2B. You can observe histone 2B-GFP behavior in live cells using fluorescent imaging with almost no cytotoxicity.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Histone 2B-GFP, BacMam 2.0, is a fusion construct of histone 2B and emGFP, providing accurate and specific targeting to cellular histone 2B-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Alexa Fluor™ 546 Phalloidin (Invitrogen™)

Alexa Fluor® 546 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, orange-fluorescent Alexa Fluor® 546 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 556/570 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 546 provides orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 546 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 546 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 546 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Histone 2B-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Histone 2B-RFP, BacMam 2.0, provides an easy way to label histone 2b with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to histone 2B. You can observe histone 2B-RFP behavior in live cells using fluorescent imaging with almost no cytotoxicity.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Histone 2B-RFP, BacMam 2.0, is a fusion construct of histone 2B and TagRFP, providing accurate and specific targeting to cellular histone 2B-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

MitoProbe™ Transition Pore Assay Kit, for flow cytometry (Invitrogen™)

The MitoProbe™ Transition Pore Assay Kit provides a proven method (Petronilli V. et al. 1989, 1999) of measuring mitochondrial permeability transition pore opening. The kit includes a dye and a quencher that are easily loaded into cells. In healthy cells, the dye, but not the quencher, enters mitochondria. The mitochondria remain brightly fluorescent until mitochondrial pore activation permits quenching of the fluorescence.

View a selection guide for all apoptosis assays for flow cytometry.

Deoxyribonuclease I, Alexa Fluor™ 488 Conjugate (Invitrogen™)

The bright green fluorescent Alexa Fluor® 488 DNase I selectively binds to G-actin (globular) and can be used for the detection of unpolymerized actin in fixed cells.

Alexa Fluor™ 633 Phalloidin (Invitrogen™)

Alexa Fluor® 633 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 633 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 632/647 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 633 provides far-red fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 633 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 633 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 633 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Lysosomes-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Lysosomes-GFP, BacMam 2.0, provides an easy way to label lysosomes with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to lamp1 (lysosomal associated membrane protein 1). You can observe lysosomes-GFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Lysosomes-GFP, BacMam 2.0, is a fusion construct of Lamp1 (lysosomal associated membrane protein 1) and emGFP, providing accurate and specific targeting to cellular lysosomes-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Premo™ FUCCI Cell Cycle Sensor (BacMam 2.0) (Invitrogen™)

Premo™ FUCCI Cell Cycle Sensor is a fluorescent, two-color sensor of cell cycle progression and division in live cells. It allows accurate and sensitive cell cycle analysis of individual cells or a population of cells by fluorescence microscopy, flow cytometry, or high-content imaging.

The Premo™ FUCCI Cell Cycle Sensor is delivered by highly efficient BacMam 2.0 technology, enabling cell cycle studies in essentially any cell type. Provided in a ready-to-use format—simply add, incubate, and image—Premo™ FUCCI affords highly efficient transient expression in cell lines, primary cells, and stem cells.

Premo™ FUCCI Cell Cycle Sensor is:

Accurate: Cell-cycle controlled expression of bright GFP and RFP indicators for live cell analysis of individual cells or populations
Highly Efficient: >90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and Convenient: Simply add Premo™ FUCCI Cell Cycle Sensor to your cells in complete medium, incubate overnight, and analyze
Safe: BacMam reagents are non-integrating and non-replicating in mammalian cells, lack observable cytopathic effects, and are suitable for biosafety level (BSL) 1 handling

Study Cell Cycle in the Relevant Cellular Context
If you are studying cell cycle regulators, cell differentiation, or are developing anti-cancer compounds against cell cycle-related targets, Premo™ FUCCI is a superbly sensitive and accurate sensor tool. The efficient transduction and transient expression allows you to conduct these experiments in the biologically relevant cell types.

Simple, Quick Cell Cycle Visualization
Simply add the ready-to-use Premo™ FUCCI cell cycle sensor to your cells in complete medium, incubate overnight, then visualize cell cycle progression in populations of cells using fluorescence microscopy, flow cytometry, or high content imaging instruments. Cells change from red in the G1 to yellow in the G1/S interphase and green in S, G2, and M phases, as fusions of emGFP and TagRFP coupled to two cell cycle-regulated proteins are expressed and degraded.

How It Works
Premo™ FUCCI is derived from the fluorescence ubiquitination cell cycle indicator (FUCCI), developed by Miyawaki and colleagues (Ref 1). FUCCI is based on two cell cycle-regulated proteins, geminin and Cdt1, fused to one green (emGFP) and red (TagRFP) fluorescent protein, respectively. As Cdt1 and geminin are present only during specific phases of the cell cycle, the fluorescent protein partner is similarly cell-cycle dependent. Ubiquitination by specific ubiquitin E3 ligases target the fusion constructs in the proteasome for degradation. E3 ligases display temporal regulation of activity, resulting in biphasic cycling of geminin and Cdt1 levels during the cell cycle. Geminin-GFP is degraded in the G1 phase; the presence of Cdt1-TagRFP is indicated by red fluorescence within nuclei. During the S, G2, and M phases, however, Cdt1 is degraded and only geminin-GFP remains, giving cells green-fluorescent nuclei. During the G1/S transition, when Cdt1 levels are decreasing and geminin levels are increasing, both proteins are present, giving a yellow-fluorescent nuclear signal. This dynamic color change (red to yellow to green) clearly displays progression through the cell cycle and division.

Powered by BacMam 2.0 Technology
BacMam technology is based on an insect virus (baculovirus) for efficient transduction and transient expression in mammalian cells. The Premo™ FUCCI cell cycle sensor uses BacMam 2.0 technology to enable cell cycle studies in essentially any cell type, from cell lines to stem cells by incorporation of a mammalian expression cassette for the FUCCI construct. Compared with BacMam 1.0 technology, BacMam 2.0 does not require the use of an enhancer and can be used in complete medium. There is no need to purify plasmid or worry about vector integrity and quality. No lipids, dye-loading chemicals, or other potentially harmful treatments are required. BacMam 2.0 incorporates elements that greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry, an enhanced CMV promoter, and the Woodchuck hepatitis post-transcriptional regulatory element (WPRE).

Titratable Expression that Lasts Up to Two Weeks
The transient transgene expression lasts from about 5 days in transformed cell lines to up to two weeks in slowly dividing cells, such as some primary cell types; we have imaged terminally differentiated neurons more than three weeks after transduction. In addition, BacMam technology permits defined optimization because it gives you the ability to precisely titrate expression levels. Baculoviruses do not replicate in mammalian cells, nor are viral genes expressed, giving them an excellent safety profile and lack of cytopathic effects on mammalian cells.

Learn more about the Premo™ FUCCI Cell Cycle Sensor

Reference:

1. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell. 2008 Feb 8;132(3):487-98. PubMed PMID: 18267078

Related products
We offer a range of BacMam-based reagents beyond CellLight® reagents, including the BacMam GFP Transduction Control that is ideal to test out the technology and optimize transduction conditions, Premo™ Biosensors, including Premo™ Autophagy Sensor, ion channel drug targets, pathway analysis kits, and more.

Learn more about these products and the BacMam technology

See other imaging tools and reagents

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Bis-ANS (4,4'-Dianilino-1,1'-Binaphthyl-5,5'-Disulfonic Acid, Dipotassium Salt) (Invitrogen™)

Bis-ANS is superior to 1-8-ANS as a probe for nonpolar cavities in proteins, often binding with an affinity that is orders-of-magnitude higher. Anilinonaphthalene sulfonates (ANS) are essentially nonfluorescent in water, only becoming appreciably fluorescent when bound to membranes. This property makes it a sensitive indicator of protein folding, conformational changes and other processes that modify the exposure of the probe to water.

CellLight™ Late Endosomes-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Late Endosomes-RFP, BacMam 2.0, provides an easy way to label late endosomes with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to Rab7a. You can observe late endosomes-RFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology

CellLight® Late Endosomes-RFP, BacMam 2.0, is a fusion construct of Rab7a and TagRFP, providing accurate and specific targeting to cellular Late Endosomes-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Deoxyribonuclease I, Alexa Fluor™ 594 Conjugate (Invitrogen™)

The bright red fluorescent Alexa Fluor® 594 DNase I selectively binds to G-actin (globular) and can be used for the detection of unpolymerized actin in fixed cells.

Alexa Fluor™ 660 Phalloidin (Invitrogen™)

Alexa Fluor® 660 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, near-infrared fluorescent Alexa Fluor® 660 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 663/690 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 660 provides near-infrared fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 660 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 660 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 660 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Lysosomes-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Lysosomes-RFP, BacMam 2.0, provides an easy way to label lysosomes with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to lamp1 (lysosomal associated membrane protein 1). You can observe lysosomes-RFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Lysosomes-RFP, BacMam 2.0, is a fusion construct of Lamp1 (lysosomal associated membrane protein 1) and TagRFP, providing accurate and specific targeting to cellular lysosomes-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Jasplakinolide (Invitrogen™)

Jasplakinolide is a macrocyclic peptide isolated from the marine sponge Jaspis johnstoni and is a potent inducer of actin polymerization in vitro by stimulating actin filament nucleation. Jasplakinolide competes with phalloidin for actin binding (Kd = 15 nM).

CellLight™ Mitochondria-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Mitochondria-GFP, BacMam 2.0, provides an easy method for the labeling of mitochondria with Green Fluorescent Protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to the Leader sequence of E1 alpha pyruvate dehydrogenase. You can observe mitochondria-GFP behavior in live cells using a fluorescent imaging system and standard FITC/GFP filter set. Study staining of mitochondria, which does not depend upon mitochondrial membrane potential. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Mitochondria-GFP, BacMam 2.0, is a fusion construct of the Leader sequence of E1 alpha pyruvate dehydrogenase and emGFP, providing accurate and specific targeting to cellular mitochondria-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Tubulin Trackerâ„¢ Deep Red (Invitrogen™)

Tubulin Tracker Deep Red provides deep-red/far-red fluorescence when bound to polymerized tubulin in live cells. Tubulin Tracker Deep Red is based on Docetaxel conjugated with a bright, photostable deep-red fluorophore. Docetaxel belongs to the family of cytoskeletal drugs that target tubulin. Tubulin Tracker Deep Red absorbs and emits optimally at 652 nm and 669 nm, respectively and can be visualized with standard Cy5 filter settings using almost any fluorescent imaging instrument. It can be multiplexed with blue, green, orange, red, and near-IR fluorophores.

CellLight™ ER-GFP, BacMam 2.0 (Invitrogen™)

CellLight® ER-GFP, BacMam 2.0, provides an easy way to label endoplasmic reticulum (ER) with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology and expresses GFP fused to the ER signal sequence of calreticulin and KDEL (ER retention signal) with minimal cellular disruption. You can observe ER-GFP behavior in live cells using fluorescent imaging and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® ER-GFP, BacMam 2.0, is a fusion construct of ER signal sequence of calreticulin and KDEL (ER retention signal) and emGFP, providing accurate and specific targeting to cellular ER-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Lissamine™ Rhodamine B 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt (rhodamine DHPE) (Invitrogen™)

The phospholipid, rhodamine DHPE is labeled on the head group with the red-fluorescent rhodamine B fluorophore with excitation/emission maxima ~560/580 nm.

CellLight™ Tubulin-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Tubulin-RFP, BacMam 2.0, provides an easy way to label tubulin with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to human tubulin. You can observe tubulin-RFP behavior in live cells with almost no cytotoxicity and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Tubulin-RFP, BacMam 2.0, is a fusion construct of human tubulin and TagRFP, providing accurate and specific targeting to cellular tubulin-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Alexa Fluor™ Plus 750 Phalloidin (Invitrogen™)

Alexa Fluor Plus 750 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, near-IR Alexa Fluor Plus 750 dye.

• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/emission: 758/784 nm. Compatible with standard Cy7 filter set.
• Superior to antibody staining
• Optimal for fixed and permeabilized samples

Get superior results in your actin staining studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides ""death cap"" mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 750 provides violet fluorescence of unparalleled brightness and photostability.

Use in multiple applications
Alexa Fluor 750 Plus Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.

SYTO™ Deep Red Nucleic Acid Stain, for live cells (Invitrogen™)

SYTO Deep Red Nucleic Acid Stain is cell-permeant dye that specifically stains the nuclei of live, dead, or fixed cells. It can be used in live-cell fluorescent imaging workflows and in fixed cell workflows, including immunocytochemistry, immunohistochemistry, and immunofluorescence experiments. After a brief incubation with the stain, the nuclei of live, dead, or fixed cells fluoresce with a deep red/far red signal that is detectable with a Cy5/deep red standard filter set or laser configuration.

• Cell-permeant dye that stains nuclei of live, dead, or fixed cells
• Detectable with deep red/Cy5 fluorecence filter set, with excitation/emission maxima of 652/669 nm
• Five times greater fluorescent signal when staining dsDNA vs RNA

SYTO Deep Red Nucleic Acid Stain can be mulitplexed with blue, green, orange, red, and near-IR fluorophores when compatible fluorescent filter/laser configurations are used. The stain increases in fluorescence with increasing concentrations of dsDNA. SYTO Deep Red Nucleic Acid Stain displays up to five times more fluorescence when incubated with dsDNA vs RNA, unlike DRAQ or other live-cell deep red nucleic acid stains. It has a bright initial signal and excellent photo stability in a typical imaging experiment. These properties make the stain ideal as a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence microscopes, fluorimeters, fluorescence microplate readers, and flow cytometers. The stain has been successfully used on live monolayer cells and spheroid cells and as a nuclear counter-stain in immunocytochemistry.

Alexa Fluor™ 680 Phalloidin (Invitrogen™)

Alexa Fluor® 680 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, near-infrared fluorescent Alexa Fluor® 680 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 679/702 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 680 provides near-infrared fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 680 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 680 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 680 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Tubulin-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Tubulin-GFP, BacMam 2.0, provides an easy method for the labeling of tubulin with Green Fluorescent Protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to human tubulin. You can observe tubulin-GFP behavior in live cells using a fluorescent imaging system and standard FITC/GFP filter set. Study dynamic action of tubulin filaments in live cells with almost no cytotoxicity. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Tubulin-GFP, BacMam 2.0, is a fusion construct of human tubulin and emGFP, providing accurate and specific targeting to cellular tubulin-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Texas Red™-X Phalloidin (Invitrogen™)

Texas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 591/608 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Texas Red®-X provides red fluorescence with superior brightness. Demonstrating very little nonspecific staining, Texas Red®-X phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Texas Red®-X phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Texas Red®-X phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

BacMam GFP Transduction Control (BacMam 2.0) (Invitrogen™)

The BacMam 2.0 Transduction Control lets you experience the power and convenience of enhanced BacMam technology, with the convenient readout of emerald GFP (emGFP); our Aequorea victoria emGFP is extremely bright and photo stable and this non-targeted form is ideal for testing transduction. Check out how well BacMam 2.0 works with GFP in your model before advancing to the more specialized reagents.

BacMam 2.0 provides several advantages over other gene transfer methods:

• Efficient: highly efficient (>90%) transduction of a wide range of mammalian cell lines, including primary cells, stem cells and neuronal cells
Fast and convenient: Simply add the BacMam 2.0 together with cells to your cover slip or microtiter plate, incubate overnight and assay – or store frozen, assay-ready cells for later use
Safe: non-replicating in mammalian cells, lack of observable cytopathic effect and biosafety level (BSL) 1 handling
Enables Flexiblity: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies and tightly control expression levels by just varying the dose

The BacMam technology is based on an insect virus (baculovirus) to help efficiently deliver and express genes in mammalian cells. The baculovirus has been modified to include an expression cassette for transgene expression in mammalian cells. BacMam 2.0 incorporate elements that help greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry and genetic elements (enhanced CMV promoter and Woodchuck Post-transcriptional Regulatory Element), that boost expression levels. Baculoviruses do not replicate in mammalian cells and thus have an excellent safety profile and lack cytopathic effects on cells.

Cell types previously not compatible with the technology (neuronal cells, T-cells), or only poorly transduced (some stem cells, CHO) are now transduced quantitatively in a simple, one step process.

We offer a range of BacMam-based reagents, including Cellular Lights™ cell labeling reagents, Premo™ Biosensors, ion channel drug targets, pathway analysis kits, and more. To learn more about these products, please visit www.invitrogen.com ⁄bacmam.

Today the other BacMam 2.0-based reagent is our enabling Premo™ Autophagy Sensor for autophagy research in live cells.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

CellLight™ Plasma Membrane-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Plasma Membrane-RFP, BacMam 2.0, provides an easy way to label the plasma membrane with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to the myristolyation/palmitoylation sequence from Lck tyrosine kinase. You can observe plasma membrane-RFP behavior in live cells without staining internal membranes, and also use multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Plasma Membrane-RFP, BacMam 2.0, is a fusion construct of the myristolyation/palmitoylation sequence from Lck tyrosine kinase and TagRFP, providing accurate and specific targeting to cellular plasma membrane-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ ER-RFP, BacMam 2.0 (Invitrogen™)

CellLight® ER-RFP, BacMam 2.0, provides an easy way to label endoplasmic reticulum (ER) with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology and expresses RFP fused to the ER signal sequence of calreticulin and KDEL (ER retention signal) with minimal cellular disruption. You can observe ER-RFP behavior in live cells using fluorescent imaging and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® ER-RFP, BacMam 2.0, is a fusion construct of ER signal sequence of calreticulin and KDEL (ER retention signal) and TagRFP, providing accurate and specific targeting to cellular ER-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Alexa Fluor™ 350 Phalloidin (Invitrogen™)

Alexa Fluor® 350 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, blue fluorescent Alexa Fluor® 350 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 346/442 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 350 provides blue fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 350 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 350 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 350 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Mitochondria-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Mitochondria-RFP, BacMam 2.0, provides an easy method for the labeling of mitochondria with Red Fluorescent Protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to the Leader sequence of E1 alpha pyruvate dehydrogenase. You can observe mitochondria-RFP behavior in live cells using a fluorescent imaging system and standard TRITC/RFP filter set. Study staining of mitochondria, which does not depend upon mitochondrial membrane potential. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Mitochondria-RFP, BacMam 2.0, is a fusion construct of the Leader sequence of E1 alpha pyruvate dehydrogenase and TagRFP, providing accurate and specific targeting to cellular mitochondria-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.