Shop All Cellular Structure Probes

Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, Bis-Acetate), for live-cell imaging (Invitrogen™)

Tubulin Tracker™ Green (Oregon Green® 488 Taxol, bis-acetate) provides green-fluorescent staining of polymerized tubulin in live cells. Tubulin Tracker™ Green is an uncharged, nonfluorescent compound that easily passes through the plasma membrane of live cells. Once inside the cell, the lipophilic blocking group is cleaved by non-specific esterases, resulting in a green-fluorescent, charged form.

Tubulin Tracker™ Deep Red (Invitrogen™)

Tubulin Tracker Deep Red provides deep-red/far-red fluorescence when bound to polymerized tubulin in live cells. Tubulin Tracker Deep Red is based on Docetaxel conjugated with a bright, photostable deep-red fluorophore. Docetaxel belongs to the family of cytoskeletal drugs that target tubulin. Tubulin Tracker Deep Red absorbs and emits optimally at 652 nm and 669 nm, respectively and can be visualized with standard Cy5 filter settings using almost any fluorescent imaging instrument. It can be multiplexed with blue, green, orange, red, and near-IR fluorophores.

Premo™ FUCCI Cell Cycle Sensor (BacMam 2.0) (Invitrogen™)

Premo™ FUCCI Cell Cycle Sensor is a fluorescent, two-color sensor of cell cycle progression and division in live cells. It allows accurate and sensitive cell cycle analysis of individual cells or a population of cells by fluorescence microscopy, flow cytometry, or high-content imaging.

At 2 x 200 µL, this is a smaller pack size version (1/5 volume) of the popular full-size product and perfect for testing its performance in your system.

The Premo™ FUCCI Cell Cycle Sensor is delivered by highly efficient BacMam 2.0 technology, enabling cell cycle studies in essentially any cell type. Provided in a ready-to-use format—simply add, incubate, and image. Premo™ FUCCI affords highly efficient transient expression in cell lines, primary cells, and stem cells.

Premo™ FUCCI Cell Cycle Sensor is:

Accurate: Cell-cycle controlled expression of bright GFP and RFP indicators for live cell analysis of individual cells or populations
Highly Efficient: >90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and Convenient: Simply add Premo™ FUCCI Cell Cycle Sensor to your cells in complete medium, incubate overnight, and analyze
Safe: BacMam reagents are non-integrating and non-replicating in mammalian cells, lack observable cytopathic effects, and are suitable for biosafety level (BSL) 1 handling

Study Cell Cycle in the Relevant Cellular Context
If you are studying cell cycle regulators, cell differentiation, or are developing anti-cancer compounds against cell cycle-related targets, Premo™ FUCCI is a superbly sensitive and accurate sensor tool. The efficient transduction and transient expression allows you to conduct these experiments in the biologically relevant cell types.

Simple, Quick Cell Cycle Visualization
Simply add the ready-to-use Premo™ FUCCI cell cycle sensor to your cells in complete medium, incubate overnight, then visualize cell cycle progression in populations of cells using fluorescence microscopy, flow cytometry, or high content imaging instruments. Cells change from red in the G1 to yellow in the G1/S interphase and green in S, G2, and M phases, as fusions of emGFP and TagRFP coupled to two cell cycle-regulated proteins are expressed and degraded.

How It Works
Premo™ FUCCI is derived from the fluorescence ubiquitination cell cycle indicator (FUCCI), developed by Miyawaki and colleagues (Ref 1). FUCCI is based on two cell cycle-regulated proteins, geminin and Cdt1, fused to one green (emGFP) and red (TagRFP) fluorescent protein, respectively. As Cdt1 and geminin are present only during specific phases of the cell cycle, the fluorescent protein partner is similarly cell-cycle dependent. Ubiquitination by specific ubiquitin E3 ligases target the fusion constructs in the proteasome for degradation. E3 ligases display temporal regulation of activity, resulting in biphasic cycling of geminin and Cdt1 levels during the cell cycle. Geminin-GFP is degraded in the G1 phase; the presence of Cdt1-TagRFP is indicated by red fluorescence within nuclei. During the S, G2, and M phases, however, Cdt1 is degraded and only geminin-GFP remains, giving cells green-fluorescent nuclei. During the G1/S transition, when Cdt1 levels are decreasing and geminin levels are increasing, both proteins are present, giving a yellow-fluorescent nuclear signal. This dynamic color change (red to yellow to green) clearly displays progression through the cell cycle and division.

Powered by BacMam 2.0 Technology
BacMam technology is based on an insect virus (baculovirus) for efficient transduction and transient expression in mammalian cells. The Premo™ FUCCI cell cycle sensor uses BacMam 2.0 technology to enable cell cycle studies in essentially any cell type, from cell lines to stem cells by incorporation of a mammalian expression cassette for the FUCCI construct. Compared with BacMam 1.0 technology, BacMam 2.0 does not require the use of an enhancer and can be used in complete medium. There is no need to purify plasmid or worry about vector integrity and quality. No lipids, dye-loading chemicals, or other potentially harmful treatments are required. BacMam 2.0 incorporates elements that greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry, an enhanced CMV promoter, and the Woodchuck hepatitis post-transcriptional regulatory element (WPRE).

Titratable Expression that Lasts Up to Two Weeks
The transient transgene expression lasts from about 5 days in transformed cell lines to up to two weeks in slowly dividing cells, such as some primary cell types; we have imaged terminally differentiated neurons more than three weeks after transduction. In addition, BacMam technology permits defined optimization because it gives you the ability to precisely titrate expression levels. Baculoviruses do not replicate in mammalian cells, nor are viral genes expressed, giving them an excellent safety profile and lack of cytopathic effects on mammalian cells.

Learn more about the Premo™ FUCCI Cell Cycle Sensor

Reference:

1. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell. 2008 Feb 8;132(3):487-98. PubMed PMID: 18267078

Related products
We offer a range of BacMam-based reagents beyond CellLight® reagents, including the BacMam GFP Transduction Control that is ideal to test out the technology and optimize transduction conditions, Premo™ Biosensors, including Premo™ Autophagy Sensor, ion channel drug targets, pathway analysis kits, and more.

Learn more about these products and the BacMam technology

See other imaging tools and reagents

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetate) (Invitrogen™)

Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetate) provides green-fluorescent staining of polymerized tubulin in live cells. Tubulin Tracker Green is an uncharged, non-fluorescent compound that easily passes through the plasma membrane of live cells. Once inside the cell, the lipophilic blocking group is cleaved by non-specific esterases, resulting in a green-fluorescent, charged form, inhibiting it from escaping the live cells. TubulinTracker Green reagent utilizes Oregon Green 488 dye, which absorbs and emits optimally at 494 nm and 522 nm, respectively, and fluorescence can be visualized with standard GFP/FITC filter settings using almost any fluorescent imaging instrument. It can be multiplex with blue, orange, red, deep-red, and near-IR fluorophores.

CellLight™ Plasma Membrane-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Plasma Membrane-GFP, BacMam 2.0, provides an easy way to label the plasma membrane with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to the myristolyation/palmitoylation sequence from Lck tyrosine kinase. You can observe plasma membrane-GFP behavior in live cells without staining internal membranes, and also use multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Plasma Membrane-GFP, BacMam 2.0, is a fusion construct of the myristolyation/palmitoylation sequence from Lck tyrosine kinase and emGFP, providing accurate and specific targeting to cellular Plasma Membrane-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Tubulin Tracker™ Variety Pack (Invitrogen™)

The Tubulin Tracker Variety Pack is a kit containing Tubulin Tracker Green and Tubulin Tracker Deep Red, which provide green and deep-red fluorescence, respectively, when bound to polymerized tubulin in live cells. Tubulin Tracker Green provides fluorescence with excitation maxima of 494 nm and emission maxima of 522 nm and can be detected with standard FITC/GFP filter settings using a fluorescence microscope. Tubulin Tracker Deep Red provides fluorescence with excitation maxima of 652 nm and emission maxima of 669 nm and can be detected using standard Cy5 filter settings.

CellLight™ Mitochondria-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Mitochondria-GFP, BacMam 2.0, provides an easy way to label mitochondria with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to the leader sequence of E1 alpha pyruvate dehydrogenase. You can observe mitochondria-GFP behavior in live cells independently of mitochondrial membrane potential and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Mitochondria-GFP, BacMam 2.0, is a fusion construct of the Leader sequence of E1 alpha pyruvate dehydrogenase and emGFP, providing accurate and specific targeting to cellular mitochondria-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ ER-RFP, BacMam 2.0 (Invitrogen™)

CellLight® ER-RFP, BacMam 2.0, provides an easy way to label endoplasmic reticulum (ER) with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology and expresses RFP fused to the ER signal sequence of calreticulin and KDEL (ER retention signal) with minimal cellular disruption. You can observe ER-RFP behavior in live cells using fluorescent imaging and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® ER-RFP, BacMam 2.0, is a fusion construct of ER signal sequence of calreticulin and KDEL (ER retention signal) and TagRFP, providing accurate and specific targeting to cellular ER-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Plasma Membrane-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Plasma Membrane-RFP, BacMam 2.0, provides an easy way to label the plasma membrane with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to the myristolyation/palmitoylation sequence from Lck tyrosine kinase. You can observe plasma membrane-RFP behavior in live cells without staining internal membranes, and also use multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Plasma Membrane-RFP, BacMam 2.0, is a fusion construct of the myristolyation/palmitoylation sequence from Lck tyrosine kinase and TagRFP, providing accurate and specific targeting to cellular plasma membrane-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Oregon Green™ 488 Phalloidin (Invitrogen™)

Oregon Green® 448 phalloidin is a high-affinity F-actin probe conjugated to green fluorescent Oregon Green® 488 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 496/520 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples
pH insensitive under physiological conditions

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Oregon Green® 488 provides green fluorescence with brightness superior to fluorescein. Oregon Green® 488 is a fluorinated analog of fluorescein with greater photostability and a lower pKa, making its fluorescence essentially pH insensitive in the physiological pH range. Demonstrating very little nonspecific staining, Oregon Green® 488 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Oregon Green® 448 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Oregon Green® 448 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Alexa Fluor™ 546 Phalloidin (Invitrogen™)

Alexa Fluor® 546 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, orange-fluorescent Alexa Fluor® 546 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 556/570 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 546 provides orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 546 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 546 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 546 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Actin-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Actin-RFP, BacMam 2.0, provides an easy method for the labeling of actin with Red Fluorescent Protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to human actin. You can observe actin-RFP behavior in live cells using a fluorescent imaging system and standard TRITC/RFP filter set. Study dynamic action of actin filaments in live cell, with almost no cytotoxicity. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Actin-RFP, BacMam 2.0, is a fusion construct of human actin and TagRFP, providing accurate and specific targeting to cellular actin-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Paclitaxel, Oregon Green™ 488 Conjugate (Oregon Green™ 488 Taxol, Flutax-2) (Invitrogen™)

This Oregon Green 488 paclitaxel derivative promises to be an important probe for labeling tubulin filaments in live cells. The fluorescent label on this new probe is attached to the 7-carbon of the paclitaxel, a strategy that permits selective binding of the probe to microtubules. Researchers have used similar fluorescent paclitaxel derivatives in a variety of systems.

Alexa Fluor™ Plus 555 Phalloidin (Invitrogen™)

Alexa Fluor Plus 555 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, orange/TRITC fluorescent Alexa Fluor Plus 555 dye. For the best possible sensitivity and specificity, Alexa Fluor Plus 555 Phalloidin is synthesized to maximize the units of Alexa Fluor Plus 555 Phalloidin per length of F-actin.

View our complete selection of Alexa Fluor and Alexa Fluor Plus conjugated phalloidin for F-actin staining ›

• Selectively stains F-actin with 3–4 times the sensitivity of Alexa Fluor 555 Phalloidin
• Outstanding fluorescence performance for sharp actin cytoskeleton images
• Excitation/emission: 555/565 nm. Compatible with standard TRITC/RFP filter set or laser settings
• More sensitive and specific than antibody staining against actin
• Optimal for fixed and permeabilized samples

Enables superior results in your actin staining studies
Phalloidin is a bi-cyclic peptide belonging to a family of toxins originating from the deadly Amanita phalloides "death cap" mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 555 dye provides orange/TRITC fluorescence of unparalleled brightness and photostability.

Use in multiple applications
Alexa Fluor Plus 555 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.

MitoProbe™ JC-1 Assay Kit (Invitrogen™)

The MitoProbe™ JC-1 Assay Kit is a quick and reliable assay used to detect changes in mitochondria by flow cytometry.

View a selection guide for all apoptosis assays for flow cytometry.

Selective to mitochondrial changes
The membrane-permeant JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. JC-1 dye exhibits potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (~529 nm) to red (~590 nm). Consequently, mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates.

Unlike other mitochondrial stains that are affected by plasma membrane potential, the ratio of green to red fluorescence of JC-1 depends only on the membrane potential and not other factors such as mitochondrial size, shape, and density, which may influence single-component fluorescence signals. Use of fluorescence ratio detection allows researchers to make comparative measurements of membrane potential and determine the percentage of mitochondria within a population that respond to an applied stimulus.

Indicator of early apoptosis
A distinctive feature of the early stages of programmed cell death is the disruption of active mitochondria. This mitochondrial disruption includes changes in the membrane potential and alterations to the oxidation-reduction potential of the mitochondria. Changes in the membrane potential are presumed to be due to the opening of the mitochondrial permeability transition pore (MPTP), allowing passage of ions and small molecules. The resulting equilibration of ions leads in turn to the decoupling of the respiratory chain and the release of cytochrome c into the cytosol.

Simple, streamlined protocol
One of the biggest challenges to working with dual-emitting dyes like JC-1 is compensation. The MitoProbe™ JC-1 Assay Kit provides a mitochondrial membrane-potential disrupter, CCCP, in order to produce compensation controls leading to a correctly compensated green-to-red fluorescence ratio. The stain protocol is simple and straightforward: incubate the cells with JC-1 dye and then analyze the fluorescent signal in the FITC and PE channels of of a 488 nm laser.

CellLight™ Mitochondria-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Mitochondria-RFP, BacMam 2.0, provides an easy way to label mitochondria with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to the leader sequence of E1 alpha pyruvate dehydrogenase. You can observe mitochondria-RFP behavior in live cells independently of mitochondrial membrane potential and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Mitochondria-RFP, BacMam 2.0, is a fusion construct of the Leader sequence of E1 alpha pyruvate dehydrogenase and TagRFP, providing accurate and specific targeting to cellular mitochondria-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Alexa Fluor™ 488 Phalloidin (Invitrogen™)

Alexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 495/518 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Your Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor® 488 provides green fluorescence of unparalleled brightness and photostability.

Use in Multiple Applications
Alexa Fluor® 488 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 488 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot® nanocrystals and other Alexa Fluor® conjugates including secondary antibodies.

Read about labeling F-Actin with phallotoxins

Find out more about our Alexa Fluor® 488 products

Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool

See the full line of Alexa Fluor® Dye products

CellLight™ Golgi-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Golgi-GFP, BacMam 2.0, provides an easy way to label golgi with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to human golgi resident enzyme (N-acetylgalactosaminyltransferase). You can observe golgi-GFP behavior in live cells using fluorescent imaging and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Golgi-GFP, BacMam 2.0, is a fusion construct of human golgi resident enzyme (N-acetylgalactosaminyltransferase) and emGFP, providing accurate and specific targeting to cellular golgi-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Jasplakinolide (Invitrogen™)

Jasplakinolide is a macrocyclic peptide isolated from the marine sponge Jaspis johnstoni and is a potent inducer of actin polymerization in vitro by stimulating actin filament nucleation. Jasplakinolide competes with phalloidin for actin binding (Kd = 15 nM).

CellLight™ Synaptophysin-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Synaptophysin-RFP, BacMam 2.0, provides an easy method for the labeling of synaptophysin with Red Fluorescent Protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to synaptophysin. You can observe synaptophysin-RFP behavior in live cells using a fluorescent imaging system and standard TRITC/RFP filter set. Study synapses in action in live neurons with minimum cellular toxicity or cellular disruption associated with neurotracers. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Synaptophysin-RFP, BacMam 2.0, is a fusion construct of synaptophysin and TagRFP, providing accurate and specific targeting to cellular synaptophysin-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

ActinRed™ 555 ReadyProbes™ Reagent (Invitrogen™)

ActinRed 555 ReadyProbes Reagent is a selective, high-affinity F-actin probe (phalloidin) conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). ActinRed 555 reagent is formulated as a room temperature-stable solution of Rhodamine Phalloidin (Cat. No. R415) and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells.

• High-affinity staining of F-actin with superior specificity compared to antibody methods
• Outstanding fluorescence performance (Ex/Em: 540/565 nm)
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area.

See other ReadyProbes reagents for cell staining
Learn more about other stains for actin

Cell imaging applications
Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, ActinRed 555 reagent allows high-contrast discrimination of actin staining.

Suggestions for use
• ActinRed 555 reagent may be added directly to fixed cells in full media or buffer solutions
• In most cases 2 drops/ml and an incubation time of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• ActinRed 555 dye is excited with a maximum at 540 nm and has an emission maximum at 565 nm. It is detected through standard TRITC (Rhodamine/DiI/Cy3/Alexa Fluor 546) filters.

Alexa Fluor™ 633 Phalloidin (Invitrogen™)

Alexa Fluor® 633 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 633 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 632/647 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 633 provides far-red fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 633 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 633 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 633 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Tubulin-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Tubulin-RFP, BacMam 2.0, provides an easy method for the labeling of tubulin with Red Fluorescent Protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to human tubulin. You can observe tubulin-RFP behavior in live cells using a fluorescent imaging system and standard TRITC/RFP filter set. Study dynamic action of tubulin filaments in live cells with almost no cytotoxicity. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Tubulin-RFP, BacMam 2.0, is a fusion construct of human tubulin and TagRFP, providing accurate and specific targeting to cellular tubulin-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Lissamine™ Rhodamine B 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt (rhodamine DHPE) (Invitrogen™)

The phospholipid, rhodamine DHPE is labeled on the head group with the red-fluorescent rhodamine B fluorophore with excitation/emission maxima ~560/580 nm.

Alexa Fluor™ 555 Phalloidin (Invitrogen™)

Alexa Fluor® 555 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red-orange fluorescent Alexa Fluor® 555 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 555/565 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 555 provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 555 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 555 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 555 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Peroxisome-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Peroxisome-GFP, BacMam 2.0, provides an easy way to label peroxisomes with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to the peroxisomal C terminal targeting sequence. You can observe peroxisome-GFP behavior in live cells and use multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Peroxisome-GFP, BacMam 2.0, is a fusion construct of peroxisomal C terminal targeting sequence and emGFP, providing accurate and specific targeting to cellular peroxisome-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Late Endosomes-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Late Endosomes-RFP, BacMam 2.0, provides an easy way to label late endosomes with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to Rab7a. You can observe late endosomes-RFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology

CellLight® Late Endosomes-RFP, BacMam 2.0, is a fusion construct of Rab7a and TagRFP, providing accurate and specific targeting to cellular Late Endosomes-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

MitoProbe™ Transition Pore Assay Kit, for flow cytometry (Invitrogen™)

The MitoProbe™ Transition Pore Assay Kit provides a proven method (Petronilli V. et al. 1989, 1999) of measuring mitochondrial permeability transition pore opening. The kit includes a dye and a quencher that are easily loaded into cells. In healthy cells, the dye, but not the quencher, enters mitochondria. The mitochondria remain brightly fluorescent until mitochondrial pore activation permits quenching of the fluorescence.

View a selection guide for all apoptosis assays for flow cytometry.

CellLight™ Nucleus-CFP, BacMam 2.0 (Invitrogen™)

CellLight® Nucleus-CFP, BacMam 2.0, provides an easy way to label nuclei with cyan fluorescent protein (CFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses CFP fused to the SV40 nuclear localization sequence. You can observe nucleus-CFP behavior in live cells without the cellular toxicity associated with intercalators and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology

CellLight® Nucleus-CFP, BacMam 2.0, is a fusion construct of SV40 nuclear localization sequence and CFP, providing accurate and specific targeting to cellular nucleus-CFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Nucleus-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Nucleus-GFP, BacMam 2.0, provides an easy way to label nuclei with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to the SV40 nuclear localization sequence. You can observe nucleus-GFP behavior in live cells without the cellular toxicity associated with intercalators and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Nucleus-GFP, BacMam 2.0, is a fusion construct of SV40 nuclear localization sequence and emGFP, providing accurate and specific targeting to cellular nucleus-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Phalloidin (Invitrogen™)

Unlabeled phalloidin can be used as a control in blocking F-acting staining or in promoting actin polymerization. can be used as a control in blocking F-acting staining or in promoting actin polymerization.

Alexa Fluor™ 568 Phalloidin (Invitrogen™)

Alexa Fluor® 568 phalloidin is a high-affinity F-actin probe conjugated to our superior Alexa Fluor® 568 dye. Alexa Fluor® 568 dye is photostable and brighter than other red-orange fluorescent dyes like Lissamine rhodamine B and Texas Red-X dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 578/600 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 568 provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 568 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 568 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 568 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Golgi-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Golgi-RFP, BacMam 2.0, provides an easy way to label golgi with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to human golgi resident enzyme (N-acetylgalactosaminyltransferase). You can observe golgi-RFP behavior in live cells using fluorescent imaging and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Golgi-RFP, BacMam 2.0, is a fusion construct of human golgi resident enzyme (N-acetylgalactosaminyltransferase) and TagRFP, providing accurate and specific targeting to cellular golgi-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Talin-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Talin-GFP, BacMam 2.0, provides an easy way to label talin with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to the c terminus of human talin. You can observe talin-GFP behavior in live cells to study the focal adhesion of integrins and their interaction with actin filaments using multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Talin-GFP, BacMam 2.0, is a fusion construct of the c terminus of human talin and emGFP, providing accurate and specific targeting to cellular talin-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

SYTO™ Deep Red Nucleic Acid Stain, for live cells (Invitrogen™)

SYTO Deep Red Nucleic Acid Stain is cell-permeant dye that specifically stains the nuclei of live, dead, or fixed cells. It can be used in live-cell fluorescent imaging workflows and in fixed cell workflows, including immunocytochemistry, immunohistochemistry, and immunofluorescence experiments. After a brief incubation with the stain, the nuclei of live, dead, or fixed cells fluoresce with a deep red/far red signal that is detectable with a Cy5/deep red standard filter set or laser configuration.

• Cell-permeant dye that stains nuclei of live, dead, or fixed cells
• Detectable with deep red/Cy5 fluorecence filter set, with excitation/emission maxima of 652/669 nm
• Five times greater fluorescent signal when staining dsDNA vs RNA

SYTO Deep Red Nucleic Acid Stain can be mulitplexed with blue, green, orange, red, and near-IR fluorophores when compatible fluorescent filter/laser configurations are used. The stain increases in fluorescence with increasing concentrations of dsDNA. SYTO Deep Red Nucleic Acid Stain displays up to five times more fluorescence when incubated with dsDNA vs RNA, unlike DRAQ or other live-cell deep red nucleic acid stains. It has a bright initial signal and excellent photo stability in a typical imaging experiment. These properties make the stain ideal as a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence microscopes, fluorimeters, fluorescence microplate readers, and flow cytometers. The stain has been successfully used on live monolayer cells and spheroid cells and as a nuclear counter-stain in immunocytochemistry.

CellLight™ Histone 2B-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Histone 2B-RFP, BacMam 2.0, provides an easy way to label histone 2b with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to histone 2B. You can observe histone 2B-RFP behavior in live cells using fluorescent imaging with almost no cytotoxicity.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Histone 2B-RFP, BacMam 2.0, is a fusion construct of histone 2B and TagRFP, providing accurate and specific targeting to cellular histone 2B-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Alexa Fluor™ Plus 405 Phalloidin (Invitrogen™)

Alexa Fluor Plus 405 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, violet-fluorescent Alexa Fluor 405 dye.

• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/emission: 405/450 nm. Compatible with standard DAPI filter set or 405/violet excitation laser.
• Superior to antibody staining
• Optimal for fixed and permeabilized samples

Get superior results in your actin staining studies
Phalloidin is a bi-cyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides ""death cap"" mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 405 provides violet fluorescence of unparalleled brightness and photostability.

Use in multiple applications
Alexa Fluor Plus 405 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.

CellLight™ Early Endosomes-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Early Endosomes-GFP, BacMam 2.0, provides an easy way to label early endosomes with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to Rab5a. You can observe early endosomes-GFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Early Endosomes-GFP, BacMam 2.0, is a fusion construct of Rab5a and emGFP, providing accurate and specific targeting to cellular Early Endosomes-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Tubulin-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Tubulin-GFP, BacMam 2.0, provides an easy way to label tubulin with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to human tubulin. You can observe tubulin-GFP behavior in live cells with almost no cytotoxicity and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Tubulin-GFP, BacMam 2.0, is a fusion construct of human tubulin and emGFP, providing accurate and specific targeting to cellular tubulin-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Alexa Fluor™ 660 Phalloidin (Invitrogen™)

Alexa Fluor® 660 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, near-infrared fluorescent Alexa Fluor® 660 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 663/690 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 660 provides near-infrared fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 660 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 660 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 660 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Lysosomes-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Lysosomes-RFP, BacMam 2.0, provides an easy method for the labeling of lysosomes with Red Fluorescent Protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to Lamp1 (lysosomal associated membrane protein 1). You can observe lysosomes-RFP behavior in live cells using a fluorescent imaging system and standard TRITC/RFP filter set. Study staining of lysosomes, which does not depend upon organelle pH or some other parameter, that can be different from one cell type to other. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Lysosomes-RFP, BacMam 2.0, is a fusion construct of Lamp1 (lysosomal associated membrane protein 1) and TagRFP, providing accurate and specific targeting to cellular lysosomes-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Lysosomes-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Lysosomes-GFP, BacMam 2.0, provides an easy method for the labeling of lysosomes with Green Fluorescent Protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to Lamp1 (lysosomal associated membrane protein 1). You can observe lysosomes-GFP behavior in live cells using a fluorescent imaging system and standard FITC/GFP filter set. Study staining of lysosomes, which does not depend upon organelle pH or some other parameter, that can be different from one cell type to other. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Lysosomes-GFP, BacMam 2.0, is a fusion construct of Lamp1 (lysosomal associated membrane protein 1) and emGFP, providing accurate and specific targeting to cellular lysosomes-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Octadecyl Rhodamine B Chloride (R18) (Invitrogen™)

The lipophilic octadecyl rhodamine B binds to membranes with the fluorophore at the aqueous interface and the alkyl tail protruding into the lipid interior.

Rhodamine Phalloidin (Invitrogen™)

Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC).

Selectively stains F-actin
Excitiation/Emission: 540/565 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples
Very widely cited fluorescent phalloidin conjugate

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).

Use in Multiple Applications
Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies. For research use only.

Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Plasma Membrane-CFP, BacMam 2.0 (Invitrogen™)

CellLight® Plasma Membrane-CFP, BacMam 2.0, provides an easy way to label the plasma membrane with cyan fluorescent protein (CFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses CFP fused to the myristolyation/palmitoylation sequence from Lck tyrosine kinase. You can observe plasma membrane-CFP behavior in live cells without staining internal membranes, and also use multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Plasma Membrane-CFP, BacMam 2.0, is a fusion construct of the myristolyation/palmitoylation sequence from Lck tyrosine kinase and CFP, providing accurate and specific targeting to cellular plasma membrane-CFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ ER-GFP, BacMam 2.0 (Invitrogen™)

CellLight® ER-GFP, BacMam 2.0, provides an easy way to label endoplasmic reticulum (ER) with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology and expresses GFP fused to the ER signal sequence of calreticulin and KDEL (ER retention signal) with minimal cellular disruption. You can observe ER-GFP behavior in live cells using fluorescent imaging and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® ER-GFP, BacMam 2.0, is a fusion construct of ER signal sequence of calreticulin and KDEL (ER retention signal) and emGFP, providing accurate and specific targeting to cellular ER-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

ActinGreen™ 488 ReadyProbes™ Reagent (Invitrogen™)

ActinGreen 488 ReadyProbes Reagent is a selective, high-affinity F-actin probe (phalloidin) conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. ActinGreen 488 reagent is formulated as a room temperature-stable solution of Alexa Fluor 488 Phalloidin (Cat. No. A12379) and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells.

• High-affinity staining of F-actin with superior specificity compared to antibody methods
• Outstanding fluorescence performance (Ex/Em: 495/518 nm)
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area

See other ReadyProbes reagents for cell staining
Learn more about other stains for actin

Cell imaging applications
Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor 488 dye provides green fluorescence of unparalleled brightness and photostability.

Suggestions for use
• ActinGreen 488 reagent may be added directly to fixed cells in full media or buffer solutions.
• In most cases, 2 drops/ml and an incubation time of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• ActinGreen 488 dye is excited with a maximum at 495 nm and has an emission maximum at 518 nm. It is detected through standard GFP and FITC filters.

Alexa Fluor™ 532 Phalloidin (Invitrogen™)

Alexa Fluor® 532 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, yellow fluorescent Alexa Fluor® 532 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 531/554 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 532 provides yellow fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor®532 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 532 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 532 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Fluorescein Phalloidin (Invitrogen™)

Fluorescein phalloidin is a high-affinity F-actin probe conjugated to the green fluorescent dye, fluorescein (FITC).

Selectively stains F-actin
Excitation/Emission: 496/516 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while fluorescein provides green fluorescence. Demonstrating very little nonspecific staining, fluorescein phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Fluorescein phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Fluorescein phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies. For research use only.

Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

MitoProbe™ DiIC1(5) Assay Kit - For Flow Cytometry - 100 Assays (Invitrogen™)

The MitoProbe™ DiIC1(5) Assay Kit provides solutions of the membrane-potential-sensitive cyanine dye DiIC1(5), and a mitochondrial membrane-potential disrupter, CCCP, for the study of mitochondrial membrane potential. DiIC1(5) penetrates the cytosol of eukaryotic cells and accumulates primarily in mitochondria with active membrane potentials, producing bright, far-red fluorescence. DiIC1(5) staining intensity decreases with decreased mitochondrial membrane potential.

View a selection guide for all apoptosis assays for flow cytometry.

5-Hexadecanoylaminofluorescein (Invitrogen™)

The lipophilic 5-hexadecanoyl fluorescein binds to membranes with the fluorophore at the aqueous interface and the alkyl tail protruding into the lipid interior.

Alexa Fluor™ 635 Phalloidin (Invitrogen™)

Alexa Fluor® 635 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 635 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 633/647 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 635 provides far-red fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 635 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 635 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 635 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Nucleus-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Nucleus-RFP, BacMam 2.0, provides an easy way to label nuclei with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to the SV40 nuclear localization sequence. You can observe nucleus-RFP behavior in live cells without the cellular toxicity associated with intercalators and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Nucleus-RFP, BacMam 2.0, is a fusion construct of SV40 nuclear localization sequence and TagRFP, providing accurate and specific targeting to cellular nucleus-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Late Endosomes-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Late Endosomes-GFP, BacMam 2.0, provides an easy way to label late endosomes with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to Rab7a. You can observe late endosomes-GFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology

CellLight® Late Endosomes-GFP, BacMam 2.0, is a fusion construct of Rab7a and emGFP, providing accurate and specific targeting to cellular Late Endosomes-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Alexa Fluor™ Plus 750 Phalloidin (Invitrogen™)

Alexa Fluor Plus 750 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, near-IR Alexa Fluor Plus 750 dye.

• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/emission: 758/784 nm. Compatible with standard Cy7 filter set.
• Superior to antibody staining
• Optimal for fixed and permeabilized samples

Get superior results in your actin staining studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides ""death cap"" mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 750 provides violet fluorescence of unparalleled brightness and photostability.

Use in multiple applications
Alexa Fluor 750 Plus Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.

CellLight™ Null (control), BacMam 2.0 (Invitrogen™)

CellLight® Null Control is intended as a negative transduction control for our CellLight® fluorescent protein live cell staining reagents. The CellLight® Null Control contains no mammalian promoter and no transgene. CellLight® reagents combine the utility and selectivity of fluorescent proteins with the transduction efficiency of BacMam technology, enabling unambiguous staining of organelles, cellular structures, and processes in live mammalian cells. They are provided in a ready-to-use format—simply add, incubate, and image—with highly efficient expression in cell lines, primary cells, stem cells, and neurons.

CellLight® Null Control is:

• Highly Efficient:
>90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
• Fast and Convenient: Simply add CellLight® Null Control reagent to your cells, incubate overnight, and evaluate how well your cells tolerate BacMam transduction
• Safe: CellLight® reagents are non-replicating in mammalian cells, lack of observable cytopathic effect, and are suitable for biosafety level (BSL) 1 handling

CellLight® Reagents Principle
Other CellLight® reagents are fusion constructs of signal peptides or cell structure proteins with premiere emGFP, TagRFP or CFP for accurate and specific targeting to sub-cellular compartments and structures. A variety of targets, including cytoskeleton, mitochondria, and secretory compartments, are available for convenient multiplexing, co-localization studies, and imaging of dynamic cellular processes where high spatial and temporal resolution is required. The CellLight® reagents tolerate fixation with formaldehyde and are therefore compatible with fixed-cell analysis.

BacMam Technology
The BacMam technology is based on an insect virus (baculovirus) for efficient transduction and transient expression in mammalian cells. The baculovirus has been modified to include an expression cassette containing the CellLight® fusion construct.

BacMam 2.0 incorporates elements that help greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry, an enhanced CMV promoter, and the Woodchuck hepatitis post-transcriptional regulatory element (WPRE).

Baculoviruses do not replicate in mammalian cells and thus have an excellent safety profile and lack cytopathic effects on cells.

Work Flow Convenience
Unlike expression vectors, BacMam reagents enable titratable and reproducible transient protein expression. There is no need for harsh transfection methods or tedious cloning. To achieve highly efficient expression even in sensitive cells, such as stem cells, neurons, and primary cells, just add CellLight® reagents to cells in complete medium, incubate, and image the next day. Alternatively, mix CellLight® reagent and cells at the time of plating.

Co-transduction efficiencies are high allowing multiple CellLight® reagents to be readily used in the same experiment when multiple structures or pathways need to be labeled. CellLight® reagents also tolerate fixation with formaldehyde and are thus compatible with antibody-based fixed-cell analysis.

Typically, transiently transduced cells express fusion protein for about five days, though in slowly dividing cells, such as some primary cell types, expression has been demonstrated for up to two weeks; in terminally differentiated neurons we have recorded images more than three weeks after transduction.

Alexa Fluor™ 594 Phalloidin (Invitrogen™)

Alexa Fluor® 594 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Alexa Fluor® 594 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 581/609 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 594 provides red fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor 594® phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 594 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 594 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Texas Red™-X Phalloidin (Invitrogen™)

Texas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 591/608 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Texas Red®-X provides red fluorescence with superior brightness. Demonstrating very little nonspecific staining, Texas Red®-X phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Texas Red®-X phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Texas Red®-X phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Alexa Fluor™ 647 Phalloidin (Invitrogen™)

Alexa Fluor® 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 647 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 650/668 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 647 provides far-red fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 647 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 647 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 647 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Alexa Fluor™ Plus 647 Phalloidin (Invitrogen™)

Alexa Fluor Plus 647 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, Cy5/deep red fluorescent Alexa Fluor Plus 647 dye. For the best possible sensitivity and specificity, Alexa Fluor Plus 647 Phalloidin is synthesized to maximize the units of Alexa Fluor Plus 647 Phalloidin per length of F-actin.

View our complete selection of Alexa Fluor and Alexa Fluor Plus conjugated phalloidin for F-actin staining ›

• Selectively stains F-actin with 2–3 times the sensitivity of Alexa Fluor 647 Phalloidin
• Outstanding fluorescence performance for sharp actin cytoskeleton images
• Excitation/emission: 650/668 nm. Compatible with standard Cy5/deep red/far red filter set or laser settings
• More sensitive and specific than antibody staining against actin
• Optimal for fixed and permeabilized samples

Enables superior results in your actin staining studies
Phalloidin is a bi-cyclic peptide belonging to a family of toxins originating from the deadly Amanita phalloides "death cap" mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 555 dye provides orange/TRITC fluorescence of unparalleled brightness and photostability.

Use in multiple applications
Alexa Fluor Plus 555 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.