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CellLight™ Null (control), BacMam 2.0 (Invitrogen™)

CellLight® Null Control is intended as a negative transduction control for our CellLight® fluorescent protein live cell staining reagents. The CellLight® Null Control contains no mammalian promoter and no transgene. CellLight® reagents combine the utility and selectivity of fluorescent proteins with the transduction efficiency of BacMam technology, enabling unambiguous staining of organelles, cellular structures, and processes in live mammalian cells. They are provided in a ready-to-use format—simply add, incubate, and image—with highly efficient expression in cell lines, primary cells, stem cells, and neurons.

CellLight® Null Control is:

• Highly Efficient:
>90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
• Fast and Convenient: Simply add CellLight® Null Control reagent to your cells, incubate overnight, and evaluate how well your cells tolerate BacMam transduction
• Safe: CellLight® reagents are non-replicating in mammalian cells, lack of observable cytopathic effect, and are suitable for biosafety level (BSL) 1 handling

CellLight® Reagents Principle
Other CellLight® reagents are fusion constructs of signal peptides or cell structure proteins with premiere emGFP, TagRFP or CFP for accurate and specific targeting to sub-cellular compartments and structures. A variety of targets, including cytoskeleton, mitochondria, and secretory compartments, are available for convenient multiplexing, co-localization studies, and imaging of dynamic cellular processes where high spatial and temporal resolution is required. The CellLight® reagents tolerate fixation with formaldehyde and are therefore compatible with fixed-cell analysis.

BacMam Technology
The BacMam technology is based on an insect virus (baculovirus) for efficient transduction and transient expression in mammalian cells. The baculovirus has been modified to include an expression cassette containing the CellLight® fusion construct.

BacMam 2.0 incorporates elements that help greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry, an enhanced CMV promoter, and the Woodchuck hepatitis post-transcriptional regulatory element (WPRE).

Baculoviruses do not replicate in mammalian cells and thus have an excellent safety profile and lack cytopathic effects on cells.

Work Flow Convenience
Unlike expression vectors, BacMam reagents enable titratable and reproducible transient protein expression. There is no need for harsh transfection methods or tedious cloning. To achieve highly efficient expression even in sensitive cells, such as stem cells, neurons, and primary cells, just add CellLight® reagents to cells in complete medium, incubate, and image the next day. Alternatively, mix CellLight® reagent and cells at the time of plating.

Co-transduction efficiencies are high allowing multiple CellLight® reagents to be readily used in the same experiment when multiple structures or pathways need to be labeled. CellLight® reagents also tolerate fixation with formaldehyde and are thus compatible with antibody-based fixed-cell analysis.

Typically, transiently transduced cells express fusion protein for about five days, though in slowly dividing cells, such as some primary cell types, expression has been demonstrated for up to two weeks; in terminally differentiated neurons we have recorded images more than three weeks after transduction.

CellLight™ Actin-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Actin-RFP, BacMam 2.0, provides an easy method for the labeling of actin with Red Fluorescent Protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to human actin. You can observe actin-RFP behavior in live cells using a fluorescent imaging system and standard TRITC/RFP filter set. Study dynamic action of actin filaments in live cell, with almost no cytotoxicity. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Actin-RFP, BacMam 2.0, is a fusion construct of human actin and TagRFP, providing accurate and specific targeting to cellular actin-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Plasma Membrane-CFP, BacMam 2.0 (Invitrogen™)

CellLight® Plasma Membrane-CFP, BacMam 2.0, provides an easy way to label the plasma membrane with cyan fluorescent protein (CFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses CFP fused to the myristolyation/palmitoylation sequence from Lck tyrosine kinase. You can observe plasma membrane-CFP behavior in live cells without staining internal membranes, and also use multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Plasma Membrane-CFP, BacMam 2.0, is a fusion construct of the myristolyation/palmitoylation sequence from Lck tyrosine kinase and CFP, providing accurate and specific targeting to cellular plasma membrane-CFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Alexa Fluor™ 647 Phalloidin (Invitrogen™)

Alexa Fluor® 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 647 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 650/668 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 647 provides far-red fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 647 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 647 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 647 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

CellLight™ Nucleus-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Nucleus-RFP, BacMam 2.0, provides an easy way to label nuclei with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to the SV40 nuclear localization sequence. You can observe nucleus-RFP behavior in live cells without the cellular toxicity associated with intercalators and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Nucleus-RFP, BacMam 2.0, is a fusion construct of SV40 nuclear localization sequence and TagRFP, providing accurate and specific targeting to cellular nucleus-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Phalloidin (Invitrogen™)

Unlabeled phalloidin can be used as a control in blocking F-acting staining or in promoting actin polymerization. can be used as a control in blocking F-acting staining or in promoting actin polymerization.

CellLight™ Actin-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Actin-GFP, BacMam 2.0, provides an easy way to label actin with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology and expresses GFP fused to human actin with almost no cytotoxic effects. Study the dynamic action of actin filaments in live cells and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Actin-GFP, BacMam 2.0, is a fusion construct of human actin and emGFP, providing accurate and specific targeting to cellular actin-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Early Endosomes-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Early Endosomes-GFP, BacMam 2.0, provides an easy way to label early endosomes with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to Rab5a. You can observe early endosomes-GFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Early Endosomes-GFP, BacMam 2.0, is a fusion construct of Rab5a and emGFP, providing accurate and specific targeting to cellular Early Endosomes-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Alexa Fluor™ 594 Phalloidin (Invitrogen™)

Alexa Fluor® 594 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Alexa Fluor® 594 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 581/609 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 594 provides red fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor 594® phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 594 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 594 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells (Invitrogen™)

SYTOX Deep Red Nucleic Acid Stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes and yet does not cross the membranes of live cells. With minimal cytoplasmic staining, SYTOX Deep Red stain is particularly useful in immunocytochemistry (ICC), immunohistochemistry (IHC), and immunofluorescence (IF) experiments for staining of nuclei of fixed cells. After a brief incubation with SYTOX Deep Red stain, the nucleic acids of dead or fixed cells fluoresce a bright, deep red/far red and can be detected with a CY5/Deep Red standard filter set or laser configuration.

Features of SYTOX Deep Red Nucleic Acid Stain include:
• Impermeant to live cells; permeant to dead or fixed cells
• Excitation/emission peak: 660/682 nm
• Use with Cy5 traditional filter set or 647 laser line
• Works with mammalian cells in mono layer or 3D cell culture, animal tissue, and bacteria
• Detectable with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers

SYTOX Deep Red Nucleic Acid Stain is multiplexable with blue, green, orange, red, and near IR fluorophores, when compatible fluorescent filter/laser configurations are used. SYTOX Deep Red stain shows increased fluorescence with increasing concentrations of dsDNA, but does not show much affinity for RNA or ssDNA. It has a bright initial signal and excellent photo stability in a typical imaging experiment. These properties make SYTOX Deep Red stain a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers.

SYTOX Deep Red Nucleic Acid Stain had been used successfully in mono-layer cells, thin-sliced tissue, thick-sliced tissue, 3D cell cultures/spheroids, and bacteria. It is supplied at a 1 mM concentration in DMSO (~2000X concentration). A concentration of 0.5 µM (1X) is recommended for most applications and cell types. In fluorescent imaging of various mammalian cells, a concentration range of 2.5 µM to 0.25 µM has been used. For viability testing by flow cytometry, a concentration of 1 µM to 10 nM has been used for Jurkat cells and a range of 4 µM to 0.5 µM has been used with E. coli.

CellLight™ Synaptophysin-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Synaptophysin-RFP, BacMam 2.0, provides an easy method for the labeling of synaptophysin with Red Fluorescent Protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to synaptophysin. You can observe synaptophysin-RFP behavior in live cells using a fluorescent imaging system and standard TRITC/RFP filter set. Study synapses in action in live neurons with minimum cellular toxicity or cellular disruption associated with neurotracers. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Synaptophysin-RFP, BacMam 2.0, is a fusion construct of synaptophysin and TagRFP, providing accurate and specific targeting to cellular synaptophysin-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Biotin-XX Phalloidin (Invitrogen™)

Biotin-XX phalloidin is a high-affinity F-actin probe conjugated to biotin-XX.

Selectively stains F-actin
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Demonstrating very little nonspecific staining, Biotin-XX phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Biotin-XX phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Biotin-XX phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetate) (Invitrogen™)

Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetate) provides green-fluorescent staining of polymerized tubulin in live cells. Tubulin Tracker Green is an uncharged, non-fluorescent compound that easily passes through the plasma membrane of live cells. Once inside the cell, the lipophilic blocking group is cleaved by non-specific esterases, resulting in a green-fluorescent, charged form, inhibiting it from escaping the live cells. TubulinTracker Green reagent utilizes Oregon Green 488 dye, which absorbs and emits optimally at 494 nm and 522 nm, respectively, and fluorescence can be visualized with standard GFP/FITC filter settings using almost any fluorescent imaging instrument. It can be multiplex with blue, orange, red, deep-red, and near-IR fluorophores.

CellLight™ Golgi-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Golgi-GFP, BacMam 2.0, provides an easy way to label golgi with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to human golgi resident enzyme (N-acetylgalactosaminyltransferase). You can observe golgi-GFP behavior in live cells using fluorescent imaging and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Golgi-GFP, BacMam 2.0, is a fusion construct of human golgi resident enzyme (N-acetylgalactosaminyltransferase) and emGFP, providing accurate and specific targeting to cellular golgi-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Alexa Fluor™ 532 Phalloidin (Invitrogen™)

Alexa Fluor® 532 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, yellow fluorescent Alexa Fluor® 532 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 531/554 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 532 provides yellow fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor®532 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 532 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 532 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.