Shop All Cellular Structure Probes

Latrunculin A (Invitrogen™)

Latrunculin A disrupts microfilament organization in cultured cells by binding to monomeric G-actin in a 1:1 complex at submicromolar concentrations. Its physiological effects include inhibition of fertilization, early embryological development and phagocytic internalization of immune complexes.

Deoxyribonuclease I, Alexa Fluor™ 488 Conjugate (Invitrogen™)

The bright green fluorescent Alexa Fluor® 488 DNase I selectively binds to G-actin (globular) and can be used for the detection of unpolymerized actin in fixed cells.

SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells (Invitrogen™)

SYTOX Deep Red Nucleic Acid Stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes and yet does not cross the membranes of live cells. With minimal cytoplasmic staining, SYTOX Deep Red stain is particularly useful in immunocytochemistry (ICC), immunohistochemistry (IHC), and immunofluorescence (IF) experiments for staining of nuclei of fixed cells. After a brief incubation with SYTOX Deep Red stain, the nucleic acids of dead or fixed cells fluoresce a bright, deep red/far red and can be detected with a CY5/Deep Red standard filter set or laser configuration.

Features of SYTOX Deep Red Nucleic Acid Stain include:
• Impermeant to live cells; permeant to dead or fixed cells
• Excitation/emission peak: 660/682 nm
• Use with Cy5 traditional filter set or 647 laser line
• Works with mammalian cells in mono layer or 3D cell culture, animal tissue, and bacteria
• Detectable with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers

SYTOX Deep Red Nucleic Acid Stain is multiplexable with blue, green, orange, red, and near IR fluorophores, when compatible fluorescent filter/laser configurations are used. SYTOX Deep Red stain shows increased fluorescence with increasing concentrations of dsDNA, but does not show much affinity for RNA or ssDNA. It has a bright initial signal and excellent photo stability in a typical imaging experiment. These properties make SYTOX Deep Red stain a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers.

SYTOX Deep Red Nucleic Acid Stain had been used successfully in mono-layer cells, thin-sliced tissue, thick-sliced tissue, 3D cell cultures/spheroids, and bacteria. It is supplied at a 1 mM concentration in DMSO (~2000X concentration). A concentration of 0.5 µM (1X) is recommended for most applications and cell types. In fluorescent imaging of various mammalian cells, a concentration range of 2.5 µM to 0.25 µM has been used. For viability testing by flow cytometry, a concentration of 1 µM to 10 nM has been used for Jurkat cells and a range of 4 µM to 0.5 µM has been used with E. coli.

Biotin-XX Phalloidin (Invitrogen™)

Biotin-XX phalloidin is a high-affinity F-actin probe conjugated to biotin-XX.

Selectively stains F-actin
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Demonstrating very little nonspecific staining, Biotin-XX phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Biotin-XX phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Biotin-XX phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.