Shop All Fluorescent Protein - Based Reagents

CellLight™ Lysosomes-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Lysosomes-GFP, BacMam 2.0, provides an easy way to label lysosomes with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to lamp1 (lysosomal associated membrane protein 1). You can observe lysosomes-GFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Lysosomes-GFP, BacMam 2.0, is a fusion construct of Lamp1 (lysosomal associated membrane protein 1) and emGFP, providing accurate and specific targeting to cellular lysosomes-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Lysosomes-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Lysosomes-RFP, BacMam 2.0, provides an easy way to label lysosomes with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to lamp1 (lysosomal associated membrane protein 1). You can observe lysosomes-RFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Lysosomes-RFP, BacMam 2.0, is a fusion construct of Lamp1 (lysosomal associated membrane protein 1) and TagRFP, providing accurate and specific targeting to cellular lysosomes-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Early Endosomes-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Early Endosomes-RFP, BacMam 2.0, provides an easy way to label early endosomes with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to Rab5a. You can observe early endosomes-RFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Early Endosomes-RFP, BacMam 2.0, is a fusion construct of Rab5a and TagRFP, providing accurate and specific targeting to cellular Early Endosomes-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Tubulin-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Tubulin-GFP, BacMam 2.0, provides an easy way to label tubulin with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to human tubulin. You can observe tubulin-GFP behavior in live cells with almost no cytotoxicity and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Tubulin-GFP, BacMam 2.0, is a fusion construct of human tubulin and emGFP, providing accurate and specific targeting to cellular tubulin-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Mitochondria-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Mitochondria-GFP, BacMam 2.0, provides an easy way to label mitochondria with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to the leader sequence of E1 alpha pyruvate dehydrogenase. You can observe mitochondria-GFP behavior in live cells independently of mitochondrial membrane potential and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Mitochondria-GFP, BacMam 2.0, is a fusion construct of the Leader sequence of E1 alpha pyruvate dehydrogenase and emGFP, providing accurate and specific targeting to cellular mitochondria-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Late Endosomes-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Late Endosomes-GFP, BacMam 2.0, provides an easy way to label late endosomes with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to Rab7a. You can observe late endosomes-GFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology

CellLight® Late Endosomes-GFP, BacMam 2.0, is a fusion construct of Rab7a and emGFP, providing accurate and specific targeting to cellular Late Endosomes-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Tubulin-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Tubulin-RFP, BacMam 2.0, provides an easy way to label tubulin with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to human tubulin. You can observe tubulin-RFP behavior in live cells with almost no cytotoxicity and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Tubulin-RFP, BacMam 2.0, is a fusion construct of human tubulin and TagRFP, providing accurate and specific targeting to cellular tubulin-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Actin-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Actin-RFP, BacMam 2.0, provides an easy way to label actin with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology and expresses RFP fused to human actin with almost no cytotoxic effects. Study the dynamic action of actin filaments in live cells and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology

CellLight® Actin-RFP, BacMam 2.0, is a fusion construct of human actin and TagRFP, providing accurate and specific targeting to cellular actin-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Mitochondria-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Mitochondria-RFP, BacMam 2.0, provides an easy way to label mitochondria with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to the leader sequence of E1 alpha pyruvate dehydrogenase. You can observe mitochondria-RFP behavior in live cells independently of mitochondrial membrane potential and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Mitochondria-RFP, BacMam 2.0, is a fusion construct of the Leader sequence of E1 alpha pyruvate dehydrogenase and TagRFP, providing accurate and specific targeting to cellular mitochondria-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Nucleus-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Nucleus-RFP, BacMam 2.0, provides an easy way to label nuclei with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to the SV40 nuclear localization sequence. You can observe nucleus-RFP behavior in live cells without the cellular toxicity associated with intercalators and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Nucleus-RFP, BacMam 2.0, is a fusion construct of SV40 nuclear localization sequence and TagRFP, providing accurate and specific targeting to cellular nucleus-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Plasma Membrane-CFP, BacMam 2.0 (Invitrogen™)

CellLight® Plasma Membrane-CFP, BacMam 2.0, provides an easy way to label the plasma membrane with cyan fluorescent protein (CFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses CFP fused to the myristolyation/palmitoylation sequence from Lck tyrosine kinase. You can observe plasma membrane-CFP behavior in live cells without staining internal membranes, and also use multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Plasma Membrane-CFP, BacMam 2.0, is a fusion construct of the myristolyation/palmitoylation sequence from Lck tyrosine kinase and CFP, providing accurate and specific targeting to cellular plasma membrane-CFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Late Endosomes-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Late Endosomes-RFP, BacMam 2.0, provides an easy way to label late endosomes with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to Rab7a. You can observe late endosomes-RFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology

CellLight® Late Endosomes-RFP, BacMam 2.0, is a fusion construct of Rab7a and TagRFP, providing accurate and specific targeting to cellular Late Endosomes-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Golgi-GFP, BacMam 2.0 (Invitrogen™)

CellLight® Golgi-GFP, BacMam 2.0, provides an easy way to label golgi with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to human golgi resident enzyme (N-acetylgalactosaminyltransferase). You can observe golgi-GFP behavior in live cells using fluorescent imaging and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Golgi-GFP, BacMam 2.0, is a fusion construct of human golgi resident enzyme (N-acetylgalactosaminyltransferase) and emGFP, providing accurate and specific targeting to cellular golgi-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

CellLight™ Plasma Membrane-RFP, BacMam 2.0 (Invitrogen™)

CellLight® Plasma Membrane-RFP, BacMam 2.0, provides an easy way to label the plasma membrane with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to the myristolyation/palmitoylation sequence from Lck tyrosine kinase. You can observe plasma membrane-RFP behavior in live cells without staining internal membranes, and also use multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Plasma Membrane-RFP, BacMam 2.0, is a fusion construct of the myristolyation/palmitoylation sequence from Lck tyrosine kinase and TagRFP, providing accurate and specific targeting to cellular plasma membrane-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Premo™ FUCCI Cell Cycle Sensor (BacMam 2.0) (Invitrogen™)

Premo™ FUCCI Cell Cycle Sensor is a fluorescent, two-color sensor of cell cycle progression and division in live cells. It allows accurate and sensitive cell cycle analysis of individual cells or a population of cells by fluorescence microscopy, flow cytometry, or high-content imaging.

At 2 x 200 µL, this is a smaller pack size version (1/5 volume) of the popular full-size product and perfect for testing its performance in your system.

The Premo™ FUCCI Cell Cycle Sensor is delivered by highly efficient BacMam 2.0 technology, enabling cell cycle studies in essentially any cell type. Provided in a ready-to-use format—simply add, incubate, and image. Premo™ FUCCI affords highly efficient transient expression in cell lines, primary cells, and stem cells.

Premo™ FUCCI Cell Cycle Sensor is:

Accurate: Cell-cycle controlled expression of bright GFP and RFP indicators for live cell analysis of individual cells or populations
Highly Efficient: >90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and Convenient: Simply add Premo™ FUCCI Cell Cycle Sensor to your cells in complete medium, incubate overnight, and analyze
Safe: BacMam reagents are non-integrating and non-replicating in mammalian cells, lack observable cytopathic effects, and are suitable for biosafety level (BSL) 1 handling

Study Cell Cycle in the Relevant Cellular Context
If you are studying cell cycle regulators, cell differentiation, or are developing anti-cancer compounds against cell cycle-related targets, Premo™ FUCCI is a superbly sensitive and accurate sensor tool. The efficient transduction and transient expression allows you to conduct these experiments in the biologically relevant cell types.

Simple, Quick Cell Cycle Visualization
Simply add the ready-to-use Premo™ FUCCI cell cycle sensor to your cells in complete medium, incubate overnight, then visualize cell cycle progression in populations of cells using fluorescence microscopy, flow cytometry, or high content imaging instruments. Cells change from red in the G1 to yellow in the G1/S interphase and green in S, G2, and M phases, as fusions of emGFP and TagRFP coupled to two cell cycle-regulated proteins are expressed and degraded.

How It Works
Premo™ FUCCI is derived from the fluorescence ubiquitination cell cycle indicator (FUCCI), developed by Miyawaki and colleagues (Ref 1). FUCCI is based on two cell cycle-regulated proteins, geminin and Cdt1, fused to one green (emGFP) and red (TagRFP) fluorescent protein, respectively. As Cdt1 and geminin are present only during specific phases of the cell cycle, the fluorescent protein partner is similarly cell-cycle dependent. Ubiquitination by specific ubiquitin E3 ligases target the fusion constructs in the proteasome for degradation. E3 ligases display temporal regulation of activity, resulting in biphasic cycling of geminin and Cdt1 levels during the cell cycle. Geminin-GFP is degraded in the G1 phase; the presence of Cdt1-TagRFP is indicated by red fluorescence within nuclei. During the S, G2, and M phases, however, Cdt1 is degraded and only geminin-GFP remains, giving cells green-fluorescent nuclei. During the G1/S transition, when Cdt1 levels are decreasing and geminin levels are increasing, both proteins are present, giving a yellow-fluorescent nuclear signal. This dynamic color change (red to yellow to green) clearly displays progression through the cell cycle and division.

Powered by BacMam 2.0 Technology
BacMam technology is based on an insect virus (baculovirus) for efficient transduction and transient expression in mammalian cells. The Premo™ FUCCI cell cycle sensor uses BacMam 2.0 technology to enable cell cycle studies in essentially any cell type, from cell lines to stem cells by incorporation of a mammalian expression cassette for the FUCCI construct. Compared with BacMam 1.0 technology, BacMam 2.0 does not require the use of an enhancer and can be used in complete medium. There is no need to purify plasmid or worry about vector integrity and quality. No lipids, dye-loading chemicals, or other potentially harmful treatments are required. BacMam 2.0 incorporates elements that greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry, an enhanced CMV promoter, and the Woodchuck hepatitis post-transcriptional regulatory element (WPRE).

Titratable Expression that Lasts Up to Two Weeks
The transient transgene expression lasts from about 5 days in transformed cell lines to up to two weeks in slowly dividing cells, such as some primary cell types; we have imaged terminally differentiated neurons more than three weeks after transduction. In addition, BacMam technology permits defined optimization because it gives you the ability to precisely titrate expression levels. Baculoviruses do not replicate in mammalian cells, nor are viral genes expressed, giving them an excellent safety profile and lack of cytopathic effects on mammalian cells.

Learn more about the Premo™ FUCCI Cell Cycle Sensor

Reference:

1. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell. 2008 Feb 8;132(3):487-98. PubMed PMID: 18267078

Related products
We offer a range of BacMam-based reagents beyond CellLight® reagents, including the BacMam GFP Transduction Control that is ideal to test out the technology and optimize transduction conditions, Premo™ Biosensors, including Premo™ Autophagy Sensor, ion channel drug targets, pathway analysis kits, and more.

Learn more about these products and the BacMam technology

See other imaging tools and reagents

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.