Shop All Fluorescent Dye - Based Reagents

Texas Red™-X Phalloidin (Invitrogen™)

Texas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 591/608 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Texas Red®-X provides red fluorescence with superior brightness. Demonstrating very little nonspecific staining, Texas Red®-X phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Texas Red®-X phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Texas Red®-X phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Alexa Fluor™ 660 Phalloidin (Invitrogen™)

Alexa Fluor® 660 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, near-infrared fluorescent Alexa Fluor® 660 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 663/690 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 660 provides near-infrared fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 660 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 660 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 660 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Paclitaxel, Oregon Green™ 488 Conjugate (Oregon Green™ 488 Taxol, Flutax-2) (Invitrogen™)

This Oregon Green 488 paclitaxel derivative promises to be an important probe for labeling tubulin filaments in live cells. The fluorescent label on this new probe is attached to the 7-carbon of the paclitaxel, a strategy that permits selective binding of the probe to microtubules. Researchers have used similar fluorescent paclitaxel derivatives in a variety of systems.

Alexa Fluor™ 680 Phalloidin (Invitrogen™)

Alexa Fluor® 680 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, near-infrared fluorescent Alexa Fluor® 680 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 679/702 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 680 provides near-infrared fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 680 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 680 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 680 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Rhodamine Phalloidin (Invitrogen™)

Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC).

Selectively stains F-actin
Excitiation/Emission: 540/565 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples
Very widely cited fluorescent phalloidin conjugate

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).

Use in Multiple Applications
Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies. For research use only.

Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Prodan (6-Propionyl-2-Dimethylaminonaphthalene) (Invitrogen™)

When the lipophlilic prodan is incorporated into membranes, its fluorescence spectra is sensitive to the physical state of the surrounding phospholipids.

Biotin-XX Phalloidin (Invitrogen™)

Biotin-XX phalloidin is a high-affinity F-actin probe conjugated to biotin-XX.

Selectively stains F-actin
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Demonstrating very little nonspecific staining, Biotin-XX phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Biotin-XX phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Biotin-XX phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Tubulin Tracker™ Deep Red (Invitrogen™)

Tubulin Tracker Deep Red provides deep-red/far-red fluorescence when bound to polymerized tubulin in live cells. Tubulin Tracker Deep Red is based on Docetaxel conjugated with a bright, photostable deep-red fluorophore. Docetaxel belongs to the family of cytoskeletal drugs that target tubulin. Tubulin Tracker Deep Red absorbs and emits optimally at 652 nm and 669 nm, respectively and can be visualized with standard Cy5 filter settings using almost any fluorescent imaging instrument. It can be multiplexed with blue, green, orange, red, and near-IR fluorophores.

5-Hexadecanoylaminofluorescein (Invitrogen™)

The lipophilic 5-hexadecanoyl fluorescein binds to membranes with the fluorophore at the aqueous interface and the alkyl tail protruding into the lipid interior.

Alexa Fluor™ 647 Phalloidin (Invitrogen™)

Alexa Fluor® 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 647 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 650/668 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 647 provides far-red fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 647 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 647 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 647 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells (Invitrogen™)

SYTOX Deep Red Nucleic Acid Stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes and yet does not cross the membranes of live cells. With minimal cytoplasmic staining, SYTOX Deep Red stain is particularly useful in immunocytochemistry (ICC), immunohistochemistry (IHC), and immunofluorescence (IF) experiments for staining of nuclei of fixed cells. After a brief incubation with SYTOX Deep Red stain, the nucleic acids of dead or fixed cells fluoresce a bright, deep red/far red and can be detected with a CY5/Deep Red standard filter set or laser configuration.

Features of SYTOX Deep Red Nucleic Acid Stain include:
• Impermeant to live cells; permeant to dead or fixed cells
• Excitation/emission peak: 660/682 nm
• Use with Cy5 traditional filter set or 647 laser line
• Works with mammalian cells in mono layer or 3D cell culture, animal tissue, and bacteria
• Detectable with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers

SYTOX Deep Red Nucleic Acid Stain is multiplexable with blue, green, orange, red, and near IR fluorophores, when compatible fluorescent filter/laser configurations are used. SYTOX Deep Red stain shows increased fluorescence with increasing concentrations of dsDNA, but does not show much affinity for RNA or ssDNA. It has a bright initial signal and excellent photo stability in a typical imaging experiment. These properties make SYTOX Deep Red stain a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers.

SYTOX Deep Red Nucleic Acid Stain had been used successfully in mono-layer cells, thin-sliced tissue, thick-sliced tissue, 3D cell cultures/spheroids, and bacteria. It is supplied at a 1 mM concentration in DMSO (~2000X concentration). A concentration of 0.5 µM (1X) is recommended for most applications and cell types. In fluorescent imaging of various mammalian cells, a concentration range of 2.5 µM to 0.25 µM has been used. For viability testing by flow cytometry, a concentration of 1 µM to 10 nM has been used for Jurkat cells and a range of 4 µM to 0.5 µM has been used with E. coli.

Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetate) (Invitrogen™)

Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetate) provides green-fluorescent staining of polymerized tubulin in live cells. Tubulin Tracker Green is an uncharged, non-fluorescent compound that easily passes through the plasma membrane of live cells. Once inside the cell, the lipophilic blocking group is cleaved by non-specific esterases, resulting in a green-fluorescent, charged form, inhibiting it from escaping the live cells. TubulinTracker Green reagent utilizes Oregon Green 488 dye, which absorbs and emits optimally at 494 nm and 522 nm, respectively, and fluorescence can be visualized with standard GFP/FITC filter settings using almost any fluorescent imaging instrument. It can be multiplex with blue, orange, red, deep-red, and near-IR fluorophores.

ActinRed™ 555 ReadyProbes™ Reagent (Invitrogen™)

ActinRed™ 555 ReadyProbes® Reagent is a selective, high-affinity F-actin probe conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). Like other ReadyProbes® reagents, ActinRed™ 555 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells.

• High-affinity staining of F-actin with superior specificity compared to antibody methods
• Outstanding fluorescence performance (Ex/Em: 540/565 nm)
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area.

See other ReadyProbes® reagents for cell staining
Learn more about other stains for actin

Cell imaging applications
Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, ActinRed™ 555 reagent allows high-contrast discrimination of actin staining.

Suggestions for use
• ActinRed™ 555 reagent may be added directly to fixed cells in full media or buffer solutions
• In most cases 2 drops/ml and an incubation time of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• ActinRed™ 555 dye is excited with a maximum at 540 nm and has an emission maximum at 565 nm. It is detected through standard TRITC (Rhodamine/DiI/Cy3™/Alexa Fluor® 546) filters.

Fluorescein Phalloidin (Invitrogen™)

Fluorescein phalloidin is a high-affinity F-actin probe conjugated to the green fluorescent dye, fluorescein (FITC).

Selectively stains F-actin
Excitation/Emission: 496/516 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while fluorescein provides green fluorescence. Demonstrating very little nonspecific staining, fluorescein phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Fluorescein phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Fluorescein phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies. For research use only.

Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

ActinGreen™ 488 ReadyProbes™ Reagent (Invitrogen™)

ActinGreen™ 488 ReadyProbes® Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. Like other ReadyProbes® reagents, the ActinGreen™ 488 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells.

• High-affinity staining of F-actin with superior specificity compared to antibody methods
• Outstanding fluorescence performance (Ex/Em: 495/518 nm)
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area

See other ReadyProbes® reagents for cell staining
Learn more about other stains for actin

Cell imaging applications
Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor® 488 dye provides green fluorescence of unparalleled brightness and photostability.

Suggestions for use
• ActinGreen™ 488 reagent may be added directly to fixed cells in full media or buffer solutions.
• In most cases, 2 drops/ml and an incubation time of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• ActinGreen™ 488 dye is excited with a maximum at 495 nm and has an emission maximum at 518 nm. It is detected through standard GFP and FITC filters.