Shop All Fluorescent Dye - Based Reagents

Deoxyribonuclease I, Alexa Fluor™ 594 Conjugate (Invitrogen™)

The bright red fluorescent Alexa Fluor® 594 DNase I selectively binds to G-actin (globular) and can be used for the detection of unpolymerized actin in fixed cells.

5-Hexadecanoylaminofluorescein (Invitrogen™)

The lipophilic 5-hexadecanoyl fluorescein binds to membranes with the fluorophore at the aqueous interface and the alkyl tail protruding into the lipid interior.

Bis-ANS (4,4'-Dianilino-1,1'-Binaphthyl-5,5'-Disulfonic Acid, Dipotassium Salt) (Invitrogen™)

Bis-ANS is superior to 1-8-ANS as a probe for nonpolar cavities in proteins, often binding with an affinity that is orders-of-magnitude higher. Anilinonaphthalene sulfonates (ANS) are essentially nonfluorescent in water, only becoming appreciably fluorescent when bound to membranes. This property makes it a sensitive indicator of protein folding, conformational changes and other processes that modify the exposure of the probe to water.

Rhodamine Phalloidin (Invitrogen™)

Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC).

Selectively stains F-actin
Excitiation/Emission: 540/565 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples
Very widely cited fluorescent phalloidin conjugate

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).

Use in Multiple Applications
Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies. For research use only.

Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

SYTO™ Deep Red Nucleic Acid Stain, for live cells (Invitrogen™)

SYTO Deep Red Nucleic Acid Stain is cell-permeant dye that specifically stains the nuclei of live, dead, or fixed cells. It can be used in live-cell fluorescent imaging workflows and in fixed cell workflows, including immunocytochemistry, immunohistochemistry, and immunofluorescence experiments. After a brief incubation with the stain, the nuclei of live, dead, or fixed cells fluoresce with a deep red/far red signal that is detectable with a Cy5/deep red standard filter set or laser configuration.

• Cell-permeant dye that stains nuclei of live, dead, or fixed cells
• Detectable with deep red/Cy5 fluorecence filter set, with excitation/emission maxima of 652/669 nm
• Five times greater fluorescent signal when staining dsDNA vs RNA

SYTO Deep Red Nucleic Acid Stain can be mulitplexed with blue, green, orange, red, and near-IR fluorophores when compatible fluorescent filter/laser configurations are used. The stain increases in fluorescence with increasing concentrations of dsDNA. SYTO Deep Red Nucleic Acid Stain displays up to five times more fluorescence when incubated with dsDNA vs RNA, unlike DRAQ or other live-cell deep red nucleic acid stains. It has a bright initial signal and excellent photo stability in a typical imaging experiment. These properties make the stain ideal as a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence microscopes, fluorimeters, fluorescence microplate readers, and flow cytometers. The stain has been successfully used on live monolayer cells and spheroid cells and as a nuclear counter-stain in immunocytochemistry.

Texas Red™-X Phalloidin (Invitrogen™)

Texas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 591/608 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Texas Red®-X provides red fluorescence with superior brightness. Demonstrating very little nonspecific staining, Texas Red®-X phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Texas Red®-X phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Texas Red®-X phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

BODIPY™ 558/568 Phalloidin (Invitrogen™)

BODIPY® 558/568 phalloidin is a high-affinity F-actin probe conjugated to our bright, red fluorescent BODIPY® 558/568 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 558/569 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while BODIPY® 558/568 provides red fluorescence with superior brightness. Demonstrating very little nonspecific staining, BODIPY® 558/568 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
BODIPY® 558/568 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. BODIPY® 558/568 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Alexa Fluor™ 680 Phalloidin (Invitrogen™)

Alexa Fluor® 680 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, near-infrared fluorescent Alexa Fluor® 680 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 679/702 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 680 provides near-infrared fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 680 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 680 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 680 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Alexa Fluor™ 568 Phalloidin (Invitrogen™)

Alexa Fluor® 568 phalloidin is a high-affinity F-actin probe conjugated to our superior Alexa Fluor® 568 dye. Alexa Fluor® 568 dye is photostable and brighter than other red-orange fluorescent dyes like Lissamine rhodamine B and Texas Red-X dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 578/600 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 568 provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 568 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 568 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 568 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Oregon Green™ 488 Phalloidin (Invitrogen™)

Oregon Green® 448 phalloidin is a high-affinity F-actin probe conjugated to green fluorescent Oregon Green® 488 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 496/520 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples
pH insensitive under physiological conditions

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Oregon Green® 488 provides green fluorescence with brightness superior to fluorescein. Oregon Green® 488 is a fluorinated analog of fluorescein with greater photostability and a lower pKa, making its fluorescence essentially pH insensitive in the physiological pH range. Demonstrating very little nonspecific staining, Oregon Green® 488 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Oregon Green® 448 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Oregon Green® 448 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

ActinGreen™ 488 ReadyProbes™ Reagent (Invitrogen™)

ActinGreen 488 ReadyProbes Reagent is a selective, high-affinity F-actin probe (phalloidin) conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. ActinGreen 488 reagent is formulated as a room temperature-stable solution of Alexa Fluor 488 Phalloidin (Cat. No. A12379) and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells.

• High-affinity staining of F-actin with superior specificity compared to antibody methods
• Outstanding fluorescence performance (Ex/Em: 495/518 nm)
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area

See other ReadyProbes reagents for cell staining
Learn more about other stains for actin

Cell imaging applications
Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor 488 dye provides green fluorescence of unparalleled brightness and photostability.

Suggestions for use
• ActinGreen 488 reagent may be added directly to fixed cells in full media or buffer solutions.
• In most cases, 2 drops/ml and an incubation time of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• ActinGreen 488 dye is excited with a maximum at 495 nm and has an emission maximum at 518 nm. It is detected through standard GFP and FITC filters.

MitoProbe™ Transition Pore Assay Kit, for flow cytometry (Invitrogen™)

The MitoProbe™ Transition Pore Assay Kit provides a proven method (Petronilli V. et al. 1989, 1999) of measuring mitochondrial permeability transition pore opening. The kit includes a dye and a quencher that are easily loaded into cells. In healthy cells, the dye, but not the quencher, enters mitochondria. The mitochondria remain brightly fluorescent until mitochondrial pore activation permits quenching of the fluorescence.

View a selection guide for all apoptosis assays for flow cytometry.

Alexa Fluor™ Plus 405 Phalloidin (Invitrogen™)

Alexa Fluor Plus 405 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, violet-fluorescent Alexa Fluor 405 dye.

• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/emission: 405/450 nm. Compatible with standard DAPI filter set or 405/violet excitation laser.
• Superior to antibody staining
• Optimal for fixed and permeabilized samples

Get superior results in your actin staining studies
Phalloidin is a bi-cyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides ""death cap"" mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 405 provides violet fluorescence of unparalleled brightness and photostability.

Use in multiple applications
Alexa Fluor Plus 405 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.

Alexa Fluor™ 647 Phalloidin (Invitrogen™)

Alexa Fluor® 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 647 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 650/668 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 647 provides far-red fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor® 647 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 647 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 647 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Alexa Fluor™ 594 Phalloidin (Invitrogen™)

Alexa Fluor® 594 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Alexa Fluor® 594 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 581/609 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 594 provides red fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor 594® phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 594 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 594 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.