Shop All Enzyme Substrates

TMB Solution (BLOT) (Invitrogen™)

TMB (Tetramethyl Benzidine) chromogen solution may be used with horseradish peroxidase (HRP)-based immunodetection procedures.

Alexa Fluor™ 647-Poly-GT Peptide Substrate

A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.
For use in combination with the LanthaScreen® Eu-PY20 Antibody (PV5696) to evaluate inhibitors in medium to high throughput screening applications.

TMB Chromogen Solution (for ELISA) (Invitrogen™)

TMB (3,3', 5,5;-tetramethylbenzidine) chromogen solution may be used as a substrate for horseradish peroxidase (HRP) in ELISAs.

Using TMB
Use this reagent as supplied, no dilution or further preparation is required. In the presence of peroxidase (HRP, POD), TMB will be converted to a blue color that can be read at OD650 nm.

ABTS, solution (Invitrogen™)

May be used as a substrate for horseradish peroxidase (HRP) in ELISAs.

ELISA-Light™ Immunoassay System with CDP-Star™ and Sapphire-II™ Substrate/Enhancer Solution (Applied Biosystems™)

The ELISA-Light™ Immunoassay System with CDP-Star® and Sapphire-II™ Substrate⁄Enhancer Solution is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

• Heterogeneous assay format increases sensitivity and dynamic range and reduces interference by test compounds in the detection portion of the assay (as compared with homogeneous assay formats)
• Chemiluminescent immunoassay detection allows greater sensitivity and improved dynamic range as compared to radioimmunoassays and immunoassays that employ colorimetric or fluorescent detection
• High sensitivity and wide dynamic range permit sensitive detection with fewer assays, because sample dilution to fit the dynamic range of the assay is unnecessary
• A variety of ready-to-use substrate formulations lets the user precisely optimize signal intensity to the exact requirements of an assay
• The kinetics of the glow-assay provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used
• Chemiluminescent immunoassay detection provides versatile applications such as sandwich immunoassays, competitive immunoassays, and DNA probe capture assays
• Non-radioactive immunoassays eliminate concerns over use of radioisotopes

Extend the Limits of Detection
The ELISA-Light™ system offers an excellent combination of high sensitivity and throughput for assays measured in a luminometer. The ELISA-Light™ system significantly extends the limits of detection of most immunoassays previously performed with colorimetric and isotopic methods.

Multiple Substrate⁄Enhancer Formulations
The kit contains reagents optimized for chemiluminescent ELISAs employing alkaline phosphatase labels and is available with a choice of ready-to-use chemiluminescent substrate formulations containing CSPD® or CDP-Star® substrate with Sapphire-II™ or Emerald-II™ luminescence enhancers. Multiple substrate⁄enhancer formulations provide options that let the user optimize specific assays or luminometers. The specific formulation that will provide the highest sensitivity for a specific assay can vary. Therefore, an ELISA sampler kit containing all four substrate⁄enhancer formulations is available to facilitate assay development.

For Research Use Only. Not for use in diagnostics procedures.

GFP-ATF2 (19-96)

A truncated version (residues 19-96) of ATF2 has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a substrate for the JNK and p38 families of kinases.

For use in combination with the LanthaScreen® Tb-anti-ATF2 [pThr71] antibody (PV4451) to evaluate inhibitors in medium to high throughput screening applications.

Galacton-Star™ Substrate (50X Concentrate) (Invitrogen™)

Galacton-Star Substrate is a chemiluminescent substrate designed for the rapid, sensitive detection of β-galactosidase in cell lysates. The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays. Light emission from Galacton-Star Substrate reaches a maximum in 60-90 minutes and remains constant for at least 1 hour. After incubation at room temperature, the signal is measured in a luminometer.

As few as 10 femtograms of β-galactosidase (20,000 molecules) are detectable. High sensitivity makes this substrate ideal for detection of weak expression and for transfection normalization with other sensitive reporter gene assays.

ELISA-Light™ Immunoassay System with CDP-Star™ and Emerald-II™ Substrate/Enhancer Solution (Applied Biosystems™)

The ELISA-Light™ Immunoassay System with CDP-Star® and Emerald-II™ Substrate⁄Enhancer Solution is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

• Heterogeneous assay format increases sensitivity and dynamic range and reduces interference by test compounds in the detection portion of the assay (as compared with homogeneous assay formats)
• Chemiluminescent immunoassay detection allows greater sensitivity and improved dynamic range as compared to radioimmunoassays and immunoassays that employ colorimetric or fluorescent detection
• High sensitivity and wide dynamic range permit sensitive detection with fewer assays, because sample dilution to fit the dynamic range of the assay is unnecessary
• A variety of ready-to-use substrate formulations lets the user precisely optimize signal intensity to the exact requirements of an assay
• The kinetics of the glow-assay provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used
• Chemiluminescent immunoassay detection provides versatile applications such as sandwich immunoassays, competitive immunoassays, and DNA probe capture assays
• Non-radioactive immunoassays eliminate concerns over use of radioisotopes

Extend the Limits of Detection
The ELISA-Light™ system offers an excellent combination of high sensitivity and throughput for assays measured in a luminometer. The ELISA-Light™ system significantly extends the limits of detection of most immunoassays previously performed with colorimetric and isotopic methods.

Multiple Substrate⁄Enhancer Formulations
The kit contains reagents optimized for chemiluminescent ELISAs employing alkaline phosphatase labels and is available with a choice of ready-to-use chemiluminescent substrate formulations containing CSPD® or CDP-Star® substrate with Sapphire-II™ or Emerald-II™ luminescence enhancers. Multiple substrate⁄enhancer formulations provide options that let the user optimize specific assays or luminometers. The specific formulation that will provide the highest sensitivity for a specific assay can vary. Therefore, an ELISA sampler kit containing all four substrate⁄enhancer formulations is available to facilitate assay development.

For Research Use Only. Not for use in diagnostics procedures.

GFP-C-Jun (1-179)

A truncated version (residues 1-179) of c-Jun has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a substrate for the JNK family of kinases.

For use in combination with the LanthaScreen® Tb-anti-c-Jun [pSer73] antibody (PV4453) to evaluate inhibitors in medium to high throughput screening applications.

AEC Solution (IHC)

This Single Solution utilizes aminoethyl carbazole (AEC), an alcohol soluble chromogen, and generates a reddish deposit at the reaction site on tissue or cell preparations. Single Solution AEC is suitable for use with manual or automated staining systems, and is provided as a liquid in a single bottle that can be stored at -8°C.

pNPP Powder (Invitrogen™)

pNPP may be used as a substrate for alkaline phosphatase (AP) in enzyme immunoassays.

Novex™ HRP Chromogenic Substrate (TMB) (Invitrogen™)

Novex® HRP Chromogenic Substrate is a ready to use single component reagent used for sensitive detection of a horse radish peroxidase (HRP) on western blot and dot blot membranes. Consisting of a ready-to-use solution of TMB, the Novex® HRP Chromogenic Substrate forms a blue precipatate up reaction with horse radish peroxidase (HRP).

Vivid™ OOMR Substrate

Vivid® Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs. Cytochrome P450 metabolism at either of two potential oxidation sites releases the highly fluorescent product. They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. When used in combination with CYP450 BACULOSOMES® Plus Reagents as part of the Vivid® CYP450 Screening Kits, the Vivid® Substrates enable rapid, robust detection of cytochrome P450 inhibitors. This particular substrate (Vivid ® OOMR Substrate) yields a product that emits a red fluorescence.

More About Vivid® CYP450 Screening Kits
Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagent. CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

>> View all Vivid® CYP450 Screening Kits

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

OPD (o-phenylenediamine dihydrochloride) (Thermo Scientific™)

Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

OPD yields a water soluble yellow-orange product when reacted with peroxidase with an absorbance maximum of 492. OPD can easily be dissolved in a substrate buffer such as Stable Peroxide Substrate Buffer (Part No. 34062).

Related Products
OPD Substrate Tablets (o-phenylenediamine dihydrochloride)

PED-A1 (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) (Phospholipase A1 Selective Substrate) (Invitrogen™)

Molecular Probes® phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2. Fluorescently labeled versions of the cleavage product generated from these substrates are also available for use as standards for evaluating percentage substrate conversion in enzymatic reactions.

Fluorogenic Phospholipase A Substrates Specifications:

• Label (Ex/Em): PED-A1
• Specificity: PLA1
• Cleavage product: D3834
• Lyophilized product can be dissolved in DMSO for use
• Fluorescence is measured either in microplate assays or using flow cytometry

Find More Probes for Lipid Metabolism and Fatty Acid Analogs
Review Fatty Acid Analogs and Phospholipids—Section 13.2 and Probes for Lipid Metabolism and Signaling—Section 17.4 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.