Shop All Beta-Glucuronidase (GUS) Substrates

Fluorescein Di-β-D-Glucuronide (FDGlcU) (Invitrogen™)

Fluorescein di-β-D-glucuronide (FDGlcU) is colorless and nonfluorescent until it is hydrlozed to the monoglucuronide and then to the highly fluorescent fluorescein.

X-GlcU, CHA (5-Bromo-4-Chloro-3-Indolyl β-D-Glucuronide, Cyclohexylammonium Salt) (Invitrogen™)

X-GlcU is a widely used chromogenic substrate for β-glucuronidase (GUS). It yields a dark blue precipitate at the site of enzymatic activity. X-GlcU is routinely used to detect GUS expression in transformed plant cells and tissues. X-GlcU can also be used to detect E. coli contamination in food and water.


• Chromogenic substrate
• Cell permeant
• Useful to detect GUS expression in transformed plant cells and tissues


Additional Information About Glycosidase Enzymes
Glycosidase enzymes exhibit very high selectivity for hydrolysis of their preferred sugars. For example, β-galactosidase rapidly hydrolyzes β-D-galactopyranosides but usually does not hydrolyze either the anomeric α-D-galactopyranosides or the isomeric β-D-glucopyranosides. Endogenous glycosidase activity is frequently used to characterize strains of microorganisms and to selectively label organelles of mammalian cells; defects in glycosidase activity are characteristic of several diseases.

In addition, glycosidases are important reporter gene markers. Specifically, lacZ, which encodes β-galactosidase, is extensively used as a reporter gene in animals and yeast, whereas the β-glucuronidase (GUS) gene is a popular reporter gene in plants. Glycosidase substrates are also used in conjunction with glycosidase-conjugated secondary detection reagents in immunohistochemical techniques and enzyme-linked immunosorbent assays (ELISAs). See all of our Molecular Probes® fluorogenic and chromogenic glycosidase substrates.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

X-Gluc (Thermo Scientific™)

Thermo Scientific X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo.

Applications

• Detection of GUS expression in plant cells and tissues
• Detection of infections caused by E. coli
• Detection of bacterial contamination in food and water samples.

Note
• Preparation of a 0.1 M stock solution in dimethylformamide or dimethylsulfoxide is recommended.