Shop All Kinase & Phosphatase Substrates

Fluorescein-P53 (Ser15) Peptide Substrate

A fluorescein-labeled peptide substrate based upon p53 containing Ser15. p53 is phosphorylated at Ser15 by a wide range of kinases including PI3K/AKT pathway kinases such DNA-PK, ATM, and ATR.

For use in combination with the LanthaScreen® Tb-anti-phospho-p53 [pSer15] antibody (PV5130) to evaluate inhibitors in medium to high throughput screening applications.

Fluorescein-Crosstide Substrate

A general substrate for Ser/Thr kinases, the Crosstide peptide substrate is based upon GSK-3alpha/beta.

For use in combination with the LanthaScreen® Tb-pCrosstide Antibody (PV3564) to evaluate inhibitors in medium to high throughput screening applications.

Fluorescein-Poly GT

A general fluorescein peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase activity.

For use in combination with the LanthaScreen® Tb-pY20 Antibody (PV3552) to evaluate inhibitors in medium to high throughput screening applications.

PI Lipid Kinase Substrate

Specifically optimized for use with the Class II (PIK3C2A & PIK3C2B) PI3 kinases. These vesicles consist of 100% phosphatidylinositol (PI). For use with the Adapta™ Universal Kinase Assay (PV5099).

GFP-C-Jun (1-179)

A truncated version (residues 1-179) of c-Jun has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a substrate for the JNK family of kinases.

For use in combination with the LanthaScreen® Tb-anti-c-Jun [pSer73] antibody (PV4453) to evaluate inhibitors in medium to high throughput screening applications.

Alexa Fluor™ 647-Poly-GT Peptide Substrate

A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.
For use in combination with the LanthaScreen® Eu-PY20 Antibody (PV5696) to evaluate inhibitors in medium to high throughput screening applications.

Fluorescein-Poly GAT

A general fluorescein peptide substrate consisting of a polymer of glutamate, alanine, and tyrosine that is used to monitor tyrosine kinase activity.

For use in combination with the LanthaScreen® Tb-pY20 Antibody (PV3552) to evaluate inhibitors in medium to high throughput screening applications.

GFP-STAT1

STAT1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for the Janus family of tyrosine kinases.

For use in combination with the LanthaScreen® Tb-anti-STAT1 [pTyr701] Antibody (PV4844) to evaluate inhibitors in medium to high throughput screening applications.

GFP-EIF2A

EIF2A has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for the EIF2AK family of kinases including PERK (EIF2AK3) and PKR (EIF2AK2).

For use in combination with the LanthaScreen® Tb-anti-eIF2alpha [pSer52] Antibody (PV4810) to evaluate inhibitors in medium to high throughput screening applications.

Sphingosine Kinase Substrate

Sphingosine substrate specifically optimized for use with the Sphingosine kinases (SPHK1 & SPHK2). For use with the Adapta™ Universal Kinase Assay (PV5099).

Fluorescein-PKC Substrate

A fluorescein-labeled peptide substrate for the Protein Kinase C (PKC) family.

For use in combination with the LanthaScreen® Tb-pSer (PKC Substrate) antibody (PV3560) to evaluate inhibitors in medium to high throughput screening applications.

Fluorescein-MAP2K1

Inactive MAP2K1 (MEK1) has been labeled with fluorescein, and is a substrate for MAPK family members.

For use in combination with the LanthaSreen™ Tb-MAP2K1 [pSer217/221] Antibody (PV4813) to evaluate inhibitors in medium to high throughput screening applications.

PIP2:PS Lipid Kinase Substrate

Specifically optimized for use with the Class I PI3 kinases (PIK3CA, PIK3CB, PIK3CD, and PIK3CG), this substrate consists of lipid vesicles of a 1:19 molar ratio of phosphoinositol-4,5-bisphosphate (PIP2) with phosphoserine (PS).

For use with the Adapta™ Universal Kinase Assay (PV5099).

DDAO Phosphate (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) Phosphate, Diammonium Salt) (Invitrogen™)

The phosphatase substrate DDAO phosphate [9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) phosphate, diammonium salt] yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ~645/660). Although the substrate itself is fluorescent (excitation/emission maxima ~460/610 nm), the difference between the substrate's excitation maximum and that of its hydrolysis product is over 200 nm, allowing the two species to be easily distinguished.

Histone H3 Peptide (1-20)

Matched and validated lipid and peptide substrates are available to complement the Adapta® Universal Kinase Assay. The assays developed with these reagents allow for primary or secondary screening of kinase inhibitors.