Shop All Kinase & Phosphatase Substrates

Fluorescein-Poly GAT

A general fluorescein peptide substrate consisting of a polymer of glutamate, alanine, and tyrosine that is used to monitor tyrosine kinase activity.

For use in combination with the LanthaScreen® Tb-pY20 Antibody (PV3552) to evaluate inhibitors in medium to high throughput screening applications.

Fluorescein Diphosphate, Tetraammonium Salt (FDP) (Invitrogen™)

FDP is a colorless and nonfluorescent substrate for alkaline phosphatases. Sequential alkaline phosphatase mediated hydrolysis of its two phosphate substituents yields weakly fluorescent fluorescein monophosphate followed by strongly fluorescent fluorescein (excitation/emission ~490/514 nm).

Fluorescein-ERM (LRRKtide)

A fluorescein-labeled peptide substrate based upon ezrin/radixin/moesin (ERM) that is phosphorylated by LRRK2 and LRRK2 mutants.

For use in combination with the LanthaScreen® Tb-anti-ERM (pLRRKtide) Antibody (PV4899) to evalute inhibitors in medium to high throughput screening applications.

Alexa Fluor™ 647-Poly-GT Peptide Substrate

A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.
For use in combination with the LanthaScreen® Eu-PY20 Antibody (PV5696) to evaluate inhibitors in medium to high throughput screening applications.

Fluorescein-PKC Substrate

A fluorescein-labeled peptide substrate for the Protein Kinase C (PKC) family.

For use in combination with the LanthaScreen® Tb-pSer (PKC Substrate) antibody (PV3560) to evaluate inhibitors in medium to high throughput screening applications.

PI:PS Lipid Kinase Substrate

Specifically optimized for use with the Class III PI3 kinase (PIK3C3 (hVPS34)) and Type III PI4 lipid kinases, this substrate can also be used as a general substrate for the Class I lipid kinases. The lipid vesicles consist of a 1:9 molar ratio of phosphatidylinositol (PI) to phosphoserine (PS).

For use with the Adapta™ Universal Kinase Assay (PV5099).

Histone H3 Peptide (1-20)

Matched and validated lipid and peptide substrates are available to complement the Adapta® Universal Kinase Assay. The assays developed with these reagents allow for primary or secondary screening of kinase inhibitors.

ERM (LRRKtide) Substrate

A peptide substrate based upon ezrin/radixin/moesin (ERM) that is phosphorylated by LRRK2 and LRRK2 mutants. For use with the Adapta™ Universal Kinase Assay (PV5099).

Fluorescein-IKK Substrate

A fluorescein-labeled peptide substrate that is primarily used with the IkappaB kinase (IKK) family.

For use in combination with the LanthaScreen® Tb-IkappaBalpha [pSer32] antibody (PV3562) to evaluate inhibitors in medium to high throughput screening applications.

GFP-EIF2A

EIF2A has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for the EIF2AK family of kinases including PERK (EIF2AK3) and PKR (EIF2AK2).

For use in combination with the LanthaScreen® Tb-anti-eIF2alpha [pSer52] Antibody (PV4810) to evaluate inhibitors in medium to high throughput screening applications.

PI(5)P Lipid Kinase Substrate (Invitrogen™)

The PI(5)P Lipid Kinase Substrate has been specifically optimized for use with Type II PIP kinases (e.g., PIP4K2A). The substrate consists of solubilized phosphatidylinositol 5-phosphate (PI(5)P) in an aqueous buffer, and is used in combination with our Adapta™ Universal Kinase Assay to help identify unique kinase inhibitors.

• Validated for use with Thermo Fisher Scientific lipid kinases
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen™ Kinase Profiling Service

Sphingosine Kinase Substrate

Sphingosine substrate specifically optimized for use with the Sphingosine kinases (SPHK1 & SPHK2). For use with the Adapta™ Universal Kinase Assay (PV5099).

PIP2:PS Lipid Kinase Substrate

Specifically optimized for use with the Class I PI3 kinases (PIK3CA, PIK3CB, PIK3CD, and PIK3CG), this substrate consists of lipid vesicles of a 1:19 molar ratio of phosphoinositol-4,5-bisphosphate (PIP2) with phosphoserine (PS).

For use with the Adapta™ Universal Kinase Assay (PV5099).

Fluorescein-Poly GT

A general fluorescein peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase activity.

For use in combination with the LanthaScreen® Tb-pY20 Antibody (PV3552) to evaluate inhibitors in medium to high throughput screening applications.

MUP (4-Methylumbelliferyl Phosphate, Free Acid) (Invitrogen™)

MUP is a fluorescent substrate for alkaline phosphatases. Alkaline phosphatase mediated hydrolysis of its phosphate substituent yields the blue-fluorescent 4-methylumbelliferyl (excitation/emission ~386/448 nm).