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Pump (for 3.5 L bottles of GelCode™ Blue Stain Reagent) (Thermo Scientific™)

This is a complementary reagent dispensing pump attachment for the 3.5 liter GelCode Blue Stain Reagent.

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GelCode™ Blue Stain Reagent

SYPRO™ Red Protein Gel Stain (5000X in DMSO) (Invitrogen™)

SYPRO Red Protein Gel Stain is a sensitive, ready-to-use fluorescent stain for proteins in 1D gels. It offers many advantages over Coomassie Blue and silver staining, including a fast, one-step staining protocol requiring no destaining; a linear dynamic range over three orders of magnitude; and very little protein-to-protein variation in staining. Stained proteins can be viewed with a standard UV or blue-light transilluminator or imaging equipment containing the appropriate filers or laser.

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Features:
Protocol—fast, one-step staining protocol requiring no destaining
• Linear quantitation range over three orders of magnitude
• Very little protein-to-protein variation in staining

UltraPure™ Ethidium Bromide, 0.625 mg/mL (Invitrogen™)

UltraPure™ Ethidium Bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and cesium chloride (CsCl) gradients. It is offered here as an aqueous solution containing 0.625 mg/mL UltraPure™ Ethidium Bromide in a convenient dropper format. This eliminates the use of pipette tips and reduces exposure to concentrated ethidium bromide solutions.

• Sensitive—detect as little as 1 ng of nucleic acid
• Versatile—detect DNA or RNA
• Easy to use—41 uL of solution is delivered per drop. One drop is sufficient for up to 50 mL agarose and two drops for up to 100 mL

UltraPure™ Ethidium Bromide is an aromatic compound with a phenanthridine core. It has excitation maxima at 300 nm and 520 nm and an emission maximum at 600 nm.

Coomassie Fluor™ Orange Protein Gel Stain (Ready-To-Use Solution) (Invitrogen™)

Molecular Probes' proprietary Coomassie Fluor Orange protein gel stain provides fast, simple, sensitive staining of proteins separated by SDS-PAGE. After electrophoresis, the gel is stained, rinsed and photographed. Staining is complete in less than an hour – no separate fixation or destaining steps are required, and there is no risk of overstaining the gel. Stained proteins can be visualized using a standard 300 nm UV transilluminator or a laser-based scanner. Coomassie Fluor Orange protein gel stain is also available in a 5 liter size (Cat. no. C-33251).

Krypton™ Fluorescent Protein Stain (Thermo Scientific™)

Thermo Scientific Krypton Fluorescent Protein Stain enables sensitive visualization with fluorescence imagers (520nm excitation; 580nm emission) of proteins separated in polyacrylamide gels or transferred to membranes.

Features of Krypton Fluorescent Protein Stain:

Intense fluorescent stain—excitation and emission maxima of 520nm and 580nm, respectively (compatible with 532nm excitation light sources and 580 to 600nm emission filters)
Instrument-compatible—effective with popular laser-based and filter-based fluorescence imagers
Broad dynamic range—linear quantitative range spans three to four orders of magnitude Sensitive—detect as little as 0.25ng protein with the standard protocol (approx. 2.5 hours); at least as sensitive as fluorescent stains from other suppliers
Fast—standard (2.5 hours) and rapid (30 minutes) protocols are significantly faster than procedures of other popular fluorescent stains
Versatile—compatible with proteomics workflows involving downstream mass spectrometry
Compact—supplied as a 10X solution to save storage space; just dilute with water before use

This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis (SDS-PAGE). Krypton Stain uses a protein-binding dye with fluorescence properties (Ex/Em = 520/580nm) suitable for visualization with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0.25ng per band in a typical mini-gel lane (greater than 2ng for the rapid staining protocol). Krypton Stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.

For best results, detect bands using visible laser-based imagers equipped with a 532nm laser light source. Although 580nm-filters are optimal for emission, 600nm-filters are also compatible. Stained gels can be imaged on any platform with the respective excitation and emission filters.

GelCode™ Blue Stain Reagent (Thermo Scientific™)

GelCode Blue Stain Reagent is a ready-to-use protein stain based on colloidal coomassie dye G-250 that provides nanogram-level detection in polyacrylamide gels and for in-blot detection.

Features:
• No methanol-acetic acid destaining required
Fast, one-step, one-hour staining—ready-to-use stain reagent; band development is visible directly in the staining tray; no background from overnight staining; flexible fixing and washing protocol; no gel shrinkage; stained gel can be dried
Water wash increases staining sensitivity—optional step provides crystal-clear gel background and makes weakly stained bands more visible

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This unique GelCode Blue Stain Reagent stains only protein and allows bands to be viewed directly in the gel during the one hour gel staining process. Because the destaining step does not fix the protein, GelCode Blue Stain is compatible with mass spectrometry analysis and N-terminal sequence analysis.

Pro-Q™ Diamond Phosphoprotein Gel Stain (Invitrogen™)

Pro-Q Diamond Phosphoprotein Gel Stain provides a convenient method for selectively staining phosphoproteins in acrylamide gels, without the need for blotting or use of phosphoprotein specific antibodies. It is ideal for the identification of kinase targets in signal transduction pathways and for phosphoproteomic studies. This fluorescent stain allows direct, in-gel detection of phosphate groups attached to tyrosine, serine, or threonine residues. Pro-Q Diamond Phosphoprotein Gel Stain can be used with standard SDS-polyacrylamide gels or with 2D gels.

The simple and reliable staining protocol delivers results in as little as 4 to 5 hours. The stain is also compatible with mass spectrometry, allowing analysis of the phosphorylation state of entire proteomes. Pro-Q Diamond Phosphoprotein Gel Stain can be used with SYPRO Ruby protein gel stain on the same gel for multiparameter staining.

Pro-Q Diamond Phosphoprotein Gel Stain detection is compatible with a visible light-scanning instruments, imaging equipment with appropriate filters, blue-LED transilluminators, or (with reduced sensitivity) 300-nm UV transilluminators.

Pro-Q™ Emerald 300 Glycoprotein Gel and Blot Stain Kit (Invitrogen™)

Pro-Q Emerald 300 Glycoprotein Stain provides direct detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. Pro-Q Emerald glycoprotein stain reacts with periodate-oxidized carbohydrate groups, creating a bright green-fluorescent signal on glycoproteins. The staining procedure requires only three steps: fixation, oxidation, and staining—no reduction step is required. Depending on the nature and degree of glycosylation, the Pro-Q Emerald 300 stain allows the detection of as little as 1 ng of a glycoprotein per band in gels, making this stain about 50 times more sensitive than the standard periodic acid–Schiff base method using acidic fuchsin dye. Signal can be visualized using a 300 nm UV transilluminator.

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This stain can be combined with general protein stains (for example SYPRO Ruby) for dichromatic detection of glycoproteins and total proteins in gel and on blots.

SYBR™ Green II RNA Gel Stain, 10,000X concentrate in DMSO (Invitrogen™)

SYBR Green II RNA gel stain is a sensitive nucleic acid gel stain that has bright fluorescence when bound to RNA and low background in gels, making it ideal for use with either formaldehyde/agarose or polyacrylamide gels using laser scanners or standard UV transilluminators. 500 µL stains 50 minigels. A 1 mL unit size (S-7568) and a special packaging (S-7586) are available.

Colloidal Blue Staining Kit (Invitrogen™)

The Colloidal Blue Staining Kit allows detection of nanogram levels of proteins in 1D or 2D PAGE gels with minimal effort and requires only water to destain. The Colloidal Blue stain uses colloidal chemistry that reduces free dye in solution and improves the protein-to-dye binding ratio. Samples are intensely stained and visible within three hours. Background staining is virtually eliminated by destaining overnight with water. The kit requires one easy solution preparation. Methanol is required in the staining step, but is not included in the kit.

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Pierce™ Mini Gel Power Staining Kit (Thermo Scientific™)

The Thermo Scientific™ Pierce™ Power Staining kits are designed for fast Coomassie dye staining of protein gels and optimized to work exclusively with the Pierce Power Stain Cassette and Pierce Power Station. The Pierce™ Mini Gel Power Staining Kit enables rapid Coomassie dye staining of proteins mini-size polyacrylamide gels and the removal of unbound stain from the gel matrix. Two mini gels having the same formulation can be accommodated and stained simultaneous in the same power stain cassette.

• High performance—performance is equivalent to, or better than, traditional Coomassie dye staining techniques
• Fast—optimized ionic solutions enable Coomassie dye staining of protein and gel destaining within 6 to 11 minutes when used with the Pierce Power Stainer
• Compatible—verified to work with commonly used precast and homemade SDS-PAGE gels

Traditional Coomassie dye staining techniques require staining for one hour to overnight and prolonged destaining for desired results. When used in conjunction with Thermo Scientific Pierce Power Stain Solution, Destain Solution, and Gel Pads, the Pierce Power Stainer enables efficient staining and destaining in 6 to 11 minutes with results equivalent to, or better than, traditional Coomassie dye staining techniques.

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Pierce™ Midi Gel Power Staining Kit

Coomassie Brilliant Blue R-250 Dye (Thermo Scientific™)

Thermo Scientific Pierce Coomassie Brilliant Blue R-250 is one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains.

Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye. Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol. In acidic conditions, the dye binds to proteins primarily through basic amino acids (primarily arginine, lysine and histidine), and the number of coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Protein-binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm).

Features of Coomassie Brilliant Blue R-250 and G-250 Dyes:

Easy detection—Develops intensely colored complexes with proteins
High sensitivity—Can determine as little as 0.5 µg/cm2 of protein present in a gel matrix
Reversible staining—Anion of Coomassie Brilliant Blue formed in the acidic staining medium combines with the protonated amino groups of proteins by electrostatic interaction; resulting complex is reversible under the proper conditions
Differentiation between bound and unbound dye—When dissolved in 0.01M citrate buffer at pH 3.0, has an absorption maximum at 555nm; protein-dye complex is characterized by a peak slightly broader than that of the free dye with a maximum at 549 nm

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Coomassie Brilliant Blue G-250 Dye

Pro-Q™ Diamond Phosphoprotein Blot Stain Kit (Invitrogen™)

The Pro-Q Diamond Phosphoprotein Blot Stain Kit provides a rapid and simple method for directly detecting phosphoproteins on polyvinylidene difluoride (PVDF) or nitrocellulose membranes. The fluorescent stain binds selectively to the phosphate moiety and binding is independent of the amino acid sequence context. Because it is a direct stain, you don't need expensive antibodies or radioisotopes. The Pro-Q Diamond Phosphoprotein Blot Stain Kit can be used to analyze the native phosphorylation state of proteins obtained from tissue specimens or from body fluids.

Pierce™ Reversible Protein Stain Kit for Nitrocellulose Membranes (Thermo Scientific™)

The Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes is a rapid and sensitive alternative to Ponceau S stain for protein detection on nitrocellulose membranes after transfer from polyacrylamide gels.

Features:
Sensitive—high-avidity, total-protein stain; lower limit of detection equals 25 to 50 ng per band
Specific—detects only protein; does not bind or interact with other electrophoresis or sample components
Protocol—stain in less than five minutes; completely erase and reverse staining in less than 15 minutes
Stability—components are stable at room temperature, saving refrigerator space and eliminating equilibration steps

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This kit for membrane staining uses a nondestructive, reversible, reliable, and sensitive method to stain and detect proteins on nitrocellulose membranes. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The stain has minimal nonspecific interactions with the membrane surface and protein-transfer reagents. The staining protocol is simple and quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed. In addition, the stain is compatible with N-terminal sequence analysis of proteins excised and eluted from membrane.

Pro-Q™ Emerald 488 Glycoprotein Gel and Blot Stain Kit (Invitrogen™)

Pro-Q Emerald 488 Glycoprotein Stain provides direct detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. Pro-Q Emerald glycoprotein stain reacts with periodate-oxidized carbohydrate groups, creating a bright green-fluorescent signal on glycoproteins. The staining procedure requires only three steps: fixation, oxidation, and staining—no reduction step is required. Depending on the nature and degree of glycosylation, the Pro-Q Emerald 488 stain allows the detection of as little as 4 ng of a glycoprotein per band in gels, making this stain about 50 times more sensitive than the standard periodic acid–Schiff base method using acidic fuchsin dye. Signal can be visualized with visible light with wavelengths near its 510 nm excitation maximum.

Compare all glycoprotein stains ›

This stain can be combined with general protein stains (for example SYPRO Ruby) for dichromatic detection of glycoproteins and total proteins in gel and on blots.