Shop All Gel & Membrane Stains

SYBR™ Safe DNA Gel Stain in 1X TAE (Invitrogen™)

SYBR Safe™ DNA gel stain was developed specifically for reduced mutagenicity—to be safer than ethidium bromide for staining DNA in agarose or acrylamide gels. SYBR Safe™ stain is not only less mutagenic than ethidium bromide, but SYBR Safe™ stain's detection sensitivity is better than that of ethidium bromide. SYBR Safe™ stain comes as a premixed solution that can be used just like an ethidium bromide solution, either in the gel during the electrophoresis run or as a post-run stain. Bound to nucleic acids, the green-fluorescent SYBR Safe™ stain has fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm.

InVision™ His-Tag In-Gel Staining Kit (Invitrogen™)

The InVision His-tag In-gel Staining Kit includes InVision His-tag In-gel Stain as well as BenchMark His-tagged Protein Standard. InVision His-tag In-gel Stain is a ready-to-use, fast, sensitive, and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis.

Features:
• Stain proteins fused to an oligohistidine sequence
• Eliminate western blotting—detect His-tagged proteins directly in the gel
• Detect nanogram levels of protein bands with a UV transilluminator or imaging equipment with appropriate filters or lasers
• Visualize results in under three hours

Compare all His-tag stains ›

Fast and easy staining
The InVision His-tag In-gel Stain protocol is easy to perform and requires very little hands-on-time, allowing for rapid and scalable protein expression screening in a variety of gel types. Following electrophoresis, the gel is fixed, incubated in the stain, washed, and the specific His-tagged fusion protein detected by exposing the gel to UV or visible light. In under three hours, nanogram levels of His-tagged fusion protein can be detected. After documenting your InVision Stain signal, total protein staining can be performed.

Multiplexed Proteomics™ Phosphoprotein Gel Stain Kit #2 (with Pro-Q™ Diamond and SYPRO™ Ruby Gel stains) (Invitrogen™)

Our new Pro-Q Diamond phosphoprotein gel stain provides a convenient method for selectively staining phosphoproteins in acrylamide gels, without the need for blotting or phosphoprotein specific antibodies and Western blot analysis. This reagent, when combined with our total protein stain – SYPRO Ruby protein gel stain – becomes a powerful combination for quantitative proteome analysis. This convenient Pro-Q Diamond phosphoprotein gel stain and SYPRO Ruby protein gel stain pack provides each stain in 200 mL amounts. This combination is also available in 1 liter amounts (M-33305).

SimplyBlue™ SafeStain (Invitrogen™)

SimplyBlue Safe Stain is a ready-to-use, fast, sensitive, and safe Coomassie G-250 stain for visualizing protein bands on polyacrylamide gels and on dry PVDF membranes. This is completely non-hazardous and does not require methanol or acetic acid fixatives or destains. Risk of hazardous exposure and unpleasant odors are eliminated, creating a healthier lab environment.

Compare all Coomassie stains ›

Easy-to-use protocol
The SimplyBlue SafeStain protocol is easy to perform and can be completed in less than three hours. For added speed, a simple microwave procedure can be completed in 12 minutes. Destaining is not required but may be performed with deionized water to achieve maximum sensitivity, especially when performing downstream analysis such as mass spectrometry or when a crystal-clear background is required.

Ethidium Bromide Solution (10 mg/mL) (Thermo Scientific™)

Thermo Scientific Pierce Ethidium Bromide is available as a concentrated (10 mg/mL) solution to accommodate most molecular biology applications for fluorescent DNA visualization.

Features of Ethidium Bromide Solution:

• A safe and convenient way to use ethidium bromide, while minimizing exposure

Related Products
Ethidium Bromide Solution (0.625 mg/mL)

UltraPure™ Ethidium Bromide, 10 mg/mL (Invitrogen™)

UltraPure™ Ethidium Bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and Caesium chloride (CsCl) gradients. It is available as an aqueous solution containing 10 mg/ml UltraPure™ Ethidium Bromide and high-purity water.
• Sensitive: Detect as little as little as 1 ng of nucleic acids in agarose gels
• Versatile: Detect DNA or RNA
• Easy to Use: Can be used as-is or diluted to the desired concentration

UltraPure™ Ethidium Bromide is an aromatic compound with a phenanthridine core (see structure below). Ethidium bromide has excitation maxima at 300 nm and 520 nm and an emission maximum at 600 nm (see fluorescence spectra).

For research use only. Not for human or animal therapeutic or diagnostic use.

Pro-Q™ Emerald 300 Glycoprotein Gel Stain Kit, with SYPRO™ Ruby Protein Gel Stain (Invitrogen™)

Molecular Probes' proprietary Pro-Q Emerald 300 and Pro-Q Emerald 488 Glycoprotein Stain Kits provide the most advanced technology available for detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. In less than three hours, it is possible to detect as little as 300 pg of glycoprotein per band, depending on the degree of glycosylation, making these stains at least 50-fold more sensitive than standard fuchsin staining.

Coomassie Fluor™ Orange Protein Gel Stain (Ready-To-Use Solution) (Invitrogen™)

Molecular Probes' proprietary Coomassie Fluor Orange protein gel stain provides fast, simple, sensitive staining of proteins separated by SDS-PAGE. After electrophoresis, the gel is stained, rinsed and photographed. Staining is complete in less than an hour – no separate fixation or destaining steps are required, and there is no risk of overstaining the gel. Stained proteins can be visualized using a standard 300 nm UV transilluminator or a laser-based scanner. Coomassie Fluor Orange protein gel stain is also available in a 5 liter size (Cat. no. C-33251).

Pro-Q™ Diamond Phosphoprotein Gel Staining Kit (includes 1 L Stain, 40 µL Standard) (Invitrogen™)

The Pro-Q Diamond Phosphoprotein Gel Staining Kit, includes 1L of the Pro-Q Diamond Phosphoprotein Gel Stain and 40 µL of the PeppermintStick™ phosphoprotein molecular weight standards. The Pro-Q® Diamond phosphoprotein gel stain is a fluorescent stain (excitation/emission maxima ~555/580 nm) that can detect as little as 1-16 ng of phosphoprotein per band with a signal that is linear over 3 orders of magnitude. The PeppermintSTick™ phosphoprotein molecular weight standards are a mixture of phosphorylated and nonphosphorylated proteins with molecular weights from 14,000 to 116,250 daltons that serve both as molecular weight markers and as positive and negative controls.

SilverQuest™ Silver Staining Kit (Invitrogen™)

The SilverQuest Silver Staining Kit provides a rapid and easy method to silver stain proteins and DNA in polyacrylamide gels. It is specifically designed to provide sensitive silver staining compatible with mass spectrometry analysis.

Compare all silver stains ›

Features of this kit:
Compatible—staining compatible with mass spectrometry analysis
Protocol—protein bands (0.3 ng of protein) can be visualized within an hour or for added speed a microwave procedure can be completed in ~30 minutes
Color-coded solutions—color-coded solutions for easy staining protocol

Mass spectrometry-compatible reagents
The SilverQuest Silver Staining Kit has been specifically designed to make protein detection compatible with mass spectrometry analysis. The SilverQuest reagent set contains a sensitizer that contains no glutaraldehyde or formaldehyde, so it leaves lysine and arginine side-chains unmodified. Additionally, the SilverQuest destaining solution effectively removes silver from protein bands in polyacrylamide gels. Peptides remain as efficient targets for in-gel trypsin digestion and gel extraction and yield better coverage during mass spectrometry analysis.

GelCode™ Blue Safe Protein Stain (Thermo Scientific™)

GelCode Blue Safe Protein Stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping.

Features:
Sensitivity—detect down to 9 ng of protein per band in 15 minutes
Safety—non-corrosive to skin, non-flammable, and safe to ship and store until activated
Additional reagents—water washes only; no acid-fixative or methanol destain solutions required
Compatibility—suitable for staining 1D and 2D polyacrylamide gels and nitrocellulose and PVDF membranes (multiple protocols provided in the instructions)
Applications—qualitative visualization, quantitative densitometry, and downstream excision and analysis by mass spectrometry
Easy to use—add and dissolve activator crystals before first use, then simply add reagent to stain gels
Stable—store non-activated or activated stain at room temperature for up to one year

Compare all Coomassie stains ›

Because the supplied activator crystals are packaged and shipped dry in a separate bottle, GelCode Blue Safe Protein Stain is classified as nonhazardous under the U.S. Department of Transportation (DOT) shipping requirements. For proper protein staining, the activator crystals must be dissolved in the staining reagent before use. Add the entire contents of the activator crystals bottle into the GelCode Blue Safe Protein Stain bottle. Dissolve the crystals by mixing end-over-end for one minute, and then allow it to remain at room temperature for five minutes before use. Store the activated stain at room temperature.

SYPRO™ Orange Protein Gel Stain (5,000X Concentrate in DMSO) (Invitrogen™)

SYPRO Orange Protein Gel Stain is a sensitive, ready-to-use fluorescent stain for total protein detection in 1D gels. The sensitivity of SYPRO Orange Protein Stain is as good as or better than traditional silver staining techniques. Stained proteins can be viewed with a standard UV or blue-light transilluminator or imaging equipment containing the appropriate filters or lasers.

Features:
Protocol—fast, one-step staining protocol requiring no destaining
• Linear quantitation range over three orders of magnitude
• Very little protein-to-protein variation in staining

Compare all fluorescent stains ›

Pro-Q™ Emerald 300 Glycoprotein Gel and Blot Stain Kit (Invitrogen™)

Pro-Q Emerald 300 Glycoprotein Stain provides direct detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. Pro-Q Emerald glycoprotein stain reacts with periodate-oxidized carbohydrate groups, creating a bright green-fluorescent signal on glycoproteins. The staining procedure requires only three steps: fixation, oxidation, and staining—no reduction step is required. Depending on the nature and degree of glycosylation, the Pro-Q Emerald 300 stain allows the detection of as little as 1 ng of a glycoprotein per band in gels, making this stain about 50 times more sensitive than the standard periodic acid–Schiff base method using acidic fuchsin dye. Signal can be visualized using a 300 nm UV transilluminator.

Compare all glycoprotein stains ›

This stain can be combined with general protein stains (for example SYPRO Ruby) for dichromatic detection of glycoproteins and total proteins in gel and on blots.

SYBR™ Green Nucleic Acid Gel Stain Starter Kit (Invitrogen™)

The SYBR Green Nucleic Acid Stain Starter Kit makes it easy for first-time users to try our two SYBR Green gel stains. The kit contains: 50 µL of SYBR Green I nucleic acid gel stain (S-7567), 50 µL of SYBR Green II RNA gel stain (S-7568) and a SYBR Green/Gold gel stain photographic filter (S-7569).

Coomassie Brilliant Blue R-250 Dye (Thermo Scientific™)

Thermo Scientific Pierce Coomassie Brilliant Blue R-250 is one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains.

Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye. Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol. In acidic conditions, the dye binds to proteins primarily through basic amino acids (primarily arginine, lysine and histidine), and the number of coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Protein-binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm).

Features of Coomassie Brilliant Blue R-250 and G-250 Dyes:

Easy detection—Develops intensely colored complexes with proteins
High sensitivity—Can determine as little as 0.5 µg/cm2 of protein present in a gel matrix
Reversible staining—Anion of Coomassie Brilliant Blue formed in the acidic staining medium combines with the protonated amino groups of proteins by electrostatic interaction; resulting complex is reversible under the proper conditions
Differentiation between bound and unbound dye—When dissolved in 0.01M citrate buffer at pH 3.0, has an absorption maximum at 555nm; protein-dye complex is characterized by a peak slightly broader than that of the free dye with a maximum at 549 nm

Related Products
Coomassie Brilliant Blue G-250 Dye