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Pierce™ Mini Gel Power Staining Kit (Thermo Scientific™)

The Thermo Scientific™ Pierce™ Power Staining kits are designed for fast Coomassie dye staining of protein gels and optimized to work exclusively with the Pierce Power Stain Cassette and Pierce Power Station. The Pierce™ Mini Gel Power Staining Kit enables rapid Coomassie dye staining of proteins mini-size polyacrylamide gels and the removal of unbound stain from the gel matrix. Two mini gels having the same formulation can be accommodated and stained simultaneous in the same power stain cassette.

• High performance—performance is equivalent to, or better than, traditional Coomassie dye staining techniques
• Fast—optimized ionic solutions enable Coomassie dye staining of protein and gel destaining within 6 to 11 minutes when used with the Pierce Power Stainer
• Compatible—verified to work with commonly used precast and homemade SDS-PAGE gels

Traditional Coomassie dye staining techniques require staining for one hour to overnight and prolonged destaining for desired results. When used in conjunction with Thermo Scientific Pierce Power Stain Solution, Destain Solution, and Gel Pads, the Pierce Power Stainer enables efficient staining and destaining in 6 to 11 minutes with results equivalent to, or better than, traditional Coomassie dye staining techniques.

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Pierce™ Midi Gel Power Staining Kit

SYPRO™ Protein Gel Stain Starter Kit (Invitrogen™)

The SYPRO Protein Gel Stain Starter Kit makes it easy for first-time users to try our three SDS-PAGE stains. The kit contains: 50 µL of SYPRO Orange dye concentrate (S-6650), 50 µL of SYPRO Red dye concentrate (S-6653), 50 µL of SYPRO Tangerine dye concentrate (S-12010) and a SYPRO protein gel stain photographic filter (S-6656).

UltraPure™ Ethidium Bromide, 10 mg/mL (Invitrogen™)

UltraPure™ Ethidium Bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and Caesium chloride (CsCl) gradients. It is available as an aqueous solution containing 10 mg/ml UltraPure™ Ethidium Bromide and high-purity water.
• Sensitive: Detect as little as little as 1 ng of nucleic acids in agarose gels
• Versatile: Detect DNA or RNA
• Easy to Use: Can be used as-is or diluted to the desired concentration

UltraPure™ Ethidium Bromide is an aromatic compound with a phenanthridine core (see structure below). Ethidium bromide has excitation maxima at 300 nm and 520 nm and an emission maximum at 600 nm (see fluorescence spectra).

For research use only. Not for human or animal therapeutic or diagnostic use.

Krypton™ Fluorescent Protein Stain (Thermo Scientific™)

Thermo Scientific Krypton Fluorescent Protein Stain enables sensitive visualization with fluorescence imagers (520nm excitation; 580nm emission) of proteins separated in polyacrylamide gels or transferred to membranes.

Features of Krypton Fluorescent Protein Stain:

Intense fluorescent stain—excitation and emission maxima of 520nm and 580nm, respectively (compatible with 532nm excitation light sources and 580 to 600nm emission filters)
Instrument-compatible—effective with popular laser-based and filter-based fluorescence imagers
Broad dynamic range—linear quantitative range spans three to four orders of magnitude Sensitive—detect as little as 0.25ng protein with the standard protocol (approx. 2.5 hours); at least as sensitive as fluorescent stains from other suppliers
Fast—standard (2.5 hours) and rapid (30 minutes) protocols are significantly faster than procedures of other popular fluorescent stains
Versatile—compatible with proteomics workflows involving downstream mass spectrometry
Compact—supplied as a 10X solution to save storage space; just dilute with water before use

This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis (SDS-PAGE). Krypton Stain uses a protein-binding dye with fluorescence properties (Ex/Em = 520/580nm) suitable for visualization with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0.25ng per band in a typical mini-gel lane (greater than 2ng for the rapid staining protocol). Krypton Stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.

For best results, detect bands using visible laser-based imagers equipped with a 532nm laser light source. Although 580nm-filters are optimal for emission, 600nm-filters are also compatible. Stained gels can be imaged on any platform with the respective excitation and emission filters.

Colloidal Blue Staining Kit (Invitrogen™)

The Colloidal Blue Staining Kit allows detection of nanogram levels of protein with minimal effort and requires only water to destain. With the Colloidal Blue Staining Kit, you get:
  • Five times more sensitivity than Coomassie® R-250—detect as little as 10 ng of BSA
  • Easy-to-use, mass spectrometry–compatible reagents
  • Great densitometry resultsThe Colloidal Blue stain uses colloidal chemistry that reduces free dye in solution and improves the protein-to-dye binding ratio. Samples are intensely stained and visible within 3 hours. Background staining is virtually eliminated by destaining overnight with water. The kit requires one easy solution preparation. Methanol is required in the staining step, but is not included in the kit.
  • Pierce™ 6xHis Protein Tag Stain Reagent Set (Thermo Scientific™)

    The Thermo Scientific Pierce 6xHis Protein Tag Stain Reagent Set is a fluorescent stain for preferential detection of histidine-tagged (His-tagged) recombinant fusion proteins in protein polyacrylamide gels.

    The unique fluorescent stain specifically detects histidine-tagged fusion proteins that have been electrophoresed in polyacrylamide gels (e.g., SDS-PAGE). With this stain, expression of His-tagged recombinant proteins can be assessed directly in-gel, eliminating membrane transfer and Western blotting steps that are typically used to confirm purity and yield of tagged proteins. Although the lower limit of detection depends on which UV lamp (300nm) and detection equipment (CCD camera best) are used, the stain is convenient for in-process assessment of His-tagged protein production and is compatible with subsequent staining with coomassie dye and other total protein stains.

    Features of the 6xHis Protein Tag Stain Reagent Set:

    Two- to three-times faster than Western blotting—get results sooner and save hours of valuable research time
    Detects directly on the gel—can eliminate the need for a membrane transfer and Western blot step for the detection of at least 0.2 µg of 6xHis-tagged protein
    Ready-to-use, two-reagent formula—no mixing, no diluting and no fuss; guarantees a simple-to-perform, mistake-free detection protocol
    Specific fluorescent detection of 6xHis-tagged proteins—see only what you want to see (CCD camera recommended for low abundance proteins; UV-transilluminator for abundant proteins)
    Compatible with double-staining—follow His-tagged protein staining with Thermo Scientific GelCode Blue Stain Reagent for a total protein profile determination

    • Stain and developer solutions (500 mL each)

    • UV lamp source (300nm) and CCD camera

    SYBR™ Safe DNA Gel Stain (Invitrogen™)

    SYBR® Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA in agarose or acrylamide gels. SYBR® Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can utilize either blue light or UV excitation.

    • Reduce your exposure to highly mutagenic ethidium bromide and harmful UV light
    • Increase your sensitivity by reducing nonspecific background fluorescence
    • Use in place of ethidium bromide for all staining applications, including RNA staining

    A better DNA stain
    SYBR® Safe DNA Gel Stain is a better nucleic acid staining reagent for all of your molecular biology needs. Not only is SYBR® Safe DNA Gel Stain better for you and the environment, it’s better for your sample and your institution. Read more as to why SYBR® Safe DNA gel stain is your better option.

    Safer than ethidium bromide
    SYBR® Safe DNA Gel Stain has been specifically formulated and evaluated in a battery of toxicity tests with results indicating so that it is less mutagenic and safer for you to work with than ethidium bromide. An independent laboratory showed reduced mutagenicity of SYBR® Safe stain compared to ethidium bromide using the Ames test (see figure). You can further decrease your exposure risk by using visible blue-light illumination with SYBR® Safe stain rather than UV illumination. This is especially valuable when performing exposure-intensive protocols like cutting bands out of gels.

    Excellent sensitivity
    SYBR® Safe stain offers excellent sensitivity in nucleic acid visualization and documentation applications with either UV excitation or blue-light excitation. When bound to nucleic acids, the green-fluorescent SYBR® Safe stain has fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm (see figure). Plus, when used with blue light illumination, SYBR® Safe stain has less background fluorescence than ethidium bromide–stained gels illuminated with UV light.

    Easy to use
    SYBR® Safe stain is supplied as a 10,000X concentrate in DMSO which can be used just like a solution of ethidium bromide. SYBR® Safe stain can be mixed into an agarose gel for staining during electrophoresis or the gel can be incubated in a solution of SYBR® Safe stain following electrophoresis. SYBR® Safe stain can be stored at room temperature in its original packaging to avoid excessive light exposure. We also offer SYBR® Safe E-Gel pre-cast agarose gels for the ultimate in convenience.

    InVision™ His-Tag In-Gel Stain (Invitrogen™)

    InVision™ His-tag In-gel Stain is a ready-to-use, fast, sensitive and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis. Using InVision™ His-tag In-gel Stain you'll:

    • Stain proteins fused to an oligohistidine sequence
    • Eliminate western blotting-detect His-tagged proteins directly in the gel
    • Detect nanogram levels of protein bands with a UV transilluminator, equipped with a standard camera, or a laser-based scanner
    • Visualize results in under 3 hours

    Convenienty provided as one ready-to-use solution, InVision™ His-tag In-gel Stain does not require mixing, dilution, or solution preparation.

    Fast and Easy Staining
    The InVision™ His-tag In-gel Stain protocol is easy to perform and requires very little hands-on-time, allowing for rapid and scalable protein expression screening in a variety of gel types. Following electrophoresis, the gel is fixed, incubated in the stain, washed, and the specific His-tagged fusion protein detected by exposing the gel to UV or visible light. In under 3 hours, nanogram levels of His-tagged fusion protein can be detected (Figure 1). After documenting your InVision™ Stain signal, total protein staining can be performed.

    SYPRO™ Ruby Protein Gel Stain (Invitrogen™)

    SYPRO® Ruby Protein Gel Stain is a highly sensitive, ready-to-use fluorescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). SYPRO® Ruby stain is ideal for use in 1D and 2D PAGE. The sensitivity of SYPRO® Ruby Gel Stain is as good as or better than the best silver staining techniques, and this stain offers several advantages over silver staining:

    • Simple staining procedure—no destaining or timed steps required
    • Linear quantitation range over three orders of magnitude
    • Compatibility with mass spectrometry and microsequencing

    Stained proteins can be viewed with a standard UV or blue-light transilluminator or with a laser scanner. For optimal sensitivity with Polaroid film, use of the SYPRO® Photographic Filter (Cat. No. S-6656) is recommended.

    Multiplexed Proteomics™ Phosphoprotein Gel Stain Kit #1 (with Pro-Q™ Diamond and SYPRO™ Ruby Gel Stains) (Invitrogen™)

    Our new Pro-Q Diamond phosphoprotein gel stain provides a convenient method for selectively staining phosphoproteins in acrylamide gels, without the need for blotting or phosphoprotein specific antibodies and Western blot analysis. This reagent, when combined with our total protein stain – SYPRO Ruby protein gel stain – becomes a powerful combination for quantitative proteome analysis. This convenient Pro-Q Diamond phosphoprotein gel stain and SYPRO Ruby protein gel stain pack provides each stain in 1 liter amounts. This combination is also available in 200 mL amounts (M-33306).

    SYPRO™ Orange Protein Gel Stain (5,000X Concentrate in DMSO) (Invitrogen™)

    SYPRO® Orange Protein Gel Stain is a sensitive, ready-to-use fluorescent stain for proteins in 1D gels. It offers many advantages over Coomassie® Blue staining:

    • Fast, one-step staining protocol requiring no destaining
    • Linear range over three orders of magnitude
    • Very little protein-to-protein variation in staining

    Using SYPRO® Orange Protein Gel Stain
    Stained proteins can be viewed with a standard UV or blue-light transilluminator or with a laser scanner. For optimal sensitivity with Polaroid film, we recommend using the SYPRO® Photographic Filter (S6656).

    Pierce™ Reversible Protein Stain Kit for Nitrocellulose Membranes (Thermo Scientific™)

    The Thermo Scientific Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes is a rapid and sensitive alternative to Ponceau S stain for protein detection on nitrocellulose membrane after transfer from polyacrylamide gels.

    Features of the Reversible Protein Stain Kit for Nitrocellulose Membranes:

    Better than Ponceau S—more sensitive, easier to document, permanent until reversed (does not fade)
    Sensitive—high-avidity, total-protein stain; lower limit of detection equals 25 to 50 ng per band
    Specific—detects only protein; does not bind or interact with other electrophoresis or sample components
    Rapid—stain in less than 5 minutes; completely erase and reverse staining in less than 15 minutes
    Stable—components are stable at room temperature, saving refrigerator space and eliminating equilibration steps

    This kit for membrane staining uses a nondestructive, reversible, reliable and sensitive method to stain and detect proteins on nitrocellulose membranes. The kit is a superior alternative to Ponceau S stains for evaluating the efficiency of protein transfer following SDS-PAGE and before immunoblotting. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The staining protocol is simple, quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed.

    The Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes has excellent avidity for a broad range of proteins, resulting in low protein-to-protein variability and enabling most proteins to be detected at very low levels (25 to 50ng per band). Pierce Membrane Stain is superior to other protein stains available for nitrocellulose membranes. For example, Ponceau S is less sensitive and results in red bands that easily fade and are difficult to photograph. Coomassie dye is a sensitive stain, but it permanently binds to proteins and can interfere with western blotting.

    The kit uses a dye that has a high affinity for protein but does not permanently bind. The stain has minimal nonspecific interactions with the membrane surface and protein-transfer reagents, and the technique is compatible with general western blot systems. The treated membrane does not interfere with conventional chemiluminescent or chromogenic detection using HRP and alkaline phosphatase substrates. In addition, the stain is compatible with N-terminal sequence analysis of proteins excised and eluted from membrane.

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    Pierce™ Reversible Protein Stain Kit for PVDF Membranes

    SYBR™ Green I Nucleic Acid Gel Stain - 10,000X concentrate in DMSO (Invitrogen™)

    SYBR® Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels. Because SYBR® Green I has greater sensitivity for dsDNA, it is especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. With exceptionally low background fluorescence and spectral characteristics that closely match light sources and filter sets in existing instruments, SYBR® Green I stain is ideal for use with laser scanners. SYBR® Green I stain provides:

    Sensitivity—at least four times greater than ethidium bromide for DNA in agarose gels
    Ease of use—gels soaked in diluted stain can be visualized without desalting
    Compatibility—with UV transilluminators, gel documentation systems, and laser scanners
    Flexibility—for use with a broad range of applications, including DNA typing, PCR-based assays, DNA damage assays, analysis of complex samples, and real-time PCR detection

    Product use
    One mL stains 100 minigels. Various pack sizes are available.

    SYPRO™ Ruby Protein Blot Stain (Invitrogen™)

    SYPRO® Ruby protein blot stain is a highly sensitive, ready-to-use fluorescent stain for proteins on nitrocellulose or PVDF membranes. It is compatible with immunostaining (Western blotting), microsequencing and mass spectrometry. Stained proteins can be viewed with a standard UV transilluminator or with a laser scanner. For optimal sensitivity with Polaroid film, use of the SYPRO protein gel stain photographic filter (S-6656) is recommended.

    Pierce™ Silver Stain for Mass Spectrometry (Thermo Scientific™)

    Thermo Scientific Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis.

    Features of Pierce Silver Stain for Mass Spectrometry :

    Sensitive and fast staining—the low-background, easy-to-use silver stain provides subnanogram sensitivity, detecting proteins at less than 0.25ng per spot in 30 minutes after fixing
    Flexible staining protocol—fix gel for 15 to 30 minutes or overnight without any affect on staining performance; stain for 1 to 30 minutes (typically 2 to 3 minutes)
    Robust—effective silver stain for even difficult-to-stain basic proteins, such as lysozyme (pI 10) and chymotrypsinogen A (pI 9.2), which are detectable at 0.2ng and 0.5ng, respectively
    Simple, trouble-free spot preparation—stained spots in excised gel-pieces are easily destained and made ready for tryptic digestion in one hour
    MS compatible—reagents and protocol are optimized to provide excellent performance in mass spectrometry following extraction of peptides from stained 1D or 2D gels (SDS-PAGE)
    Complete kit—contains all reagents necessary for staining and destaining before in-gel proteolysis and peptide recovery for analysis by mass spectrometry
    Convenient—kit-components are stable at room temperature; saves valuable refrigerator space and eliminates the need to equilibrate reagents before use

    This silver staining kit for mass spectrometry (MS) bundles components of the high-performance Pierce Silver Stain Kit with optimized reagents to destain spots for subsequent in-gel tryptic digestion to recover peptide fragments for proteomics analysis. The resulting MS-compatible product and protocol deliver outstanding sensitivity and maintains favorable conditions for the high-yield recovery and identification (sequence coverage) of proteins by mass spectrometry. Silver staining of 2D gels is now an important intermediate step in a set of procedures that leads ultimately to identification of specific proteins in the proteome by mass fingerprinting methods. The Pierce Silver Stain protocol provides peak staining performance, flexibility, reliability and robustness for applications such as MALDI-MS.

    The Pierce Silver Stain Kit for Mass Spectrometry enables both first-time and experienced users to achieve consistent and reliable staining using high-, low- and gradient-percentage polyacrylamide gels in single-dimension and 2D formats. The optimized staining method ensures extremely sensitive staining while minimizing covalent crosslinking of protein to the gel matrix, which can inhibit protein-peptide recovery following in-gel proteolysis. The destaining reagents facilitate complete removal of silver from stained protein bands and maximum protein recovery for subsequent mass spectrometry analysis.

    Silver staining methods generally use either glutaraldehyde or formaldehyde, which cause some covalent crosslinking of protein to each other and the gel matrix. To the extent that this crosslinking occurs, extraction or elution of protein from the gel will be inhibited. Pierce Silver Stain uses formaldehyde in the stain and developer working solutions. However, the procedure accompanying the Kit for Mass Spectrometry is optimized to maximize polypeptide recovery without greatly sacrificing sensitivity.