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SYTOX™ Blue Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

SYTOX® Blue nucleic acid stain is an excellent blue-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. The excitation/emission maxima for the dye bound to DNA are 444/480 nm.

SYTO™ Blue Fluorescent Nucleic Acid Stain Sampler Kit (SYTO™ dyes 40, 41, 42, 45) (Invitrogen™)

The SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit contains a collection of cell-permeant, blue-fluorescent SYTO nucleic acid stains (SYTO® dyes 40, 41, 42, 45). Because the dyes may demonstrate different staining behaviors with various tissues and cells, it may be necessary to test the dyes to find the optimal dye for a specific application. The kit contains 50 µL of each SYTO dye.

SYTO™ 40 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 40 blue fluorescent nucleic acid stain exhibits bright, blue fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary from one cell type to another, we offer the SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit (S-11350) to enable researchers to find the dye that works best for a particular application.

SYTO™ 41 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 41 blue fluorescent nucleic acid stain exhibits bright, blue fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary from one cell type to another, we offer the SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit (S-11350) to enable researchers to find the dye that works best for a particular application.

SYTO™ 16 Green Fluorescent Nucleic Acid Stain - 1 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 16 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 (S-7572) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

eBioscience™ Fixable Viability Dye eFluor™ 506/780 Sample Pack (Invitrogen™)

Fixable Viability Dye eFluor™ 506 and 780 are viability dyes that can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells. Thus, using Fixable Viability Dyes allows dead cells to be excluded from analysis when intracellular targets are being studied. Fixable Viability Dyes may be used to label cells from all species.

Fixable Viability Dye eFluor™ 506 can be excited by the violet (405 nm) laser line and has a peak emission of 506 nm that can be detected using a 510/50 band pass filter (equivalent to AmCyan). Fixable Viability Dye eFluor™ 780 can be excited by the red (633 nm) laser line and has a peak emission of 780 nm that can be detected using a 780/60 band pass filter (equivalent to APC-eFluor™ 780 or APC-Alexa Fluor™ 750). Please make sure that your instrument is capable of detecting these dyes. For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye. If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.

Fixable Viability Dye eFluor™ 506 and 780 are supplied as a pre-diluted solutions prepared in high-quality, anhydrous DMSO. They should be protected from light and moisture. Store at less than or equal to -70°C with dessicant. They may be freeze-thawed up to 20 times. Allow vials to equilibrate to room temperature before opening.

Reported Application
Flow Cytometric Analysis

SYTO™ Orange Fluorescent Nucleic Acid Stain Sampler Kit - SYTO™ dyes 80-85 - 50 µL each (Invitrogen™)

The SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit contains our collection of cell-permeant, orange-fluorescent SYTO nucleic acid stains. Because the dyes may demonstrate different staining behaviors with various tissues and cells, it may be necessary to test the dyes to find the optimal dye for a specific application. The kit contains 50 µL each of SYTO 80—85 dyes.

SYTO™ 17 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 17 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.

Sulforhodamine 101 (Invitrogen™)

Sulforhodamine 101 is a water-soluble, nonfixable red fluorescent (excitation/emission maxima ~ 586/605 nm) polar tracer that can be used for investigating neuronal morphology, preparing fluorescent liposomes, studying cell-cell communications and following phagosome-lysosome uptake and fusion.

FluoVolt™ Membrane Potential Kit (Invitrogen™)

The FluoVolt™ Membrane Potential Kit represents the next generation in voltage sensitive fluorescent probe-based kits. The FluoVolt™ probe brings together the best characteristics of the fast and slow response membrane potential fluorescent probes. The FluoVolt™ probe responds to changes in membrane potential in sub-milliseconds and displays a high magnitude of response.

Features of the FluoVolt™ Membrane Potential Probe include:

• Fast—responds to changes in membrane potential within sub-milliseconds
• High sensitivity—response range is typically 25% per 100 mV
• Emission/excitation works with standard FITC settings
• Can be used in imaging or patch clamp applications

Changes in membrane potential play a central role in many physiological processes, including nerve-impulse propagation, muscle contraction, and cell signaling. Potentiometric fluorescent probes are important tools for studying these processes and are generally characterized as slow- or fast-response probes.

The Best Characteristics of Slow- and Fast-Response Probes
Slow-response probes function by entering depolarized cells, binding to proteins or membranes, and exhibiting enhanced fluorescence. This membrane translocation event decreases the ability of these reporters to respond to changes in membrane potential and introduces a capacitive load that can affect cell health. However, these probes display a high magnitude of response; typically in the 1% per mV range.

Fast-response probes are molecules that change their structure in response to the surrounding electric field and detect transient (millisecond) potential changes. However, when compared to the slow-response probes, the fast-response probes have a magnitude of potential-dependent fluorescence change that is often small (2–10% fluorescence change per 100 mV).

The FluoVolt™ Membrane Potential Probe displays the best properties of the slow- and fast-response probes. The FluoVolt™ probe is a fast-response probe with a superior potential-dependent fluorescence response. The response is fast enough to detect transient (sub-millisecond) potential changes in excitable cells and generates a signal change in excess of 25% per 100 mV.

PowerLoad™ and Background Suppressor Solutions Also Included
For easy cell loading, the FluoVolt™ Membrane Potential Kit contains PowerLoad™ Concentrate. Due to the unique nature of the PowerLoad™ solution, it can be used in the presence of complete culture media, thus reducing the negative effects of replacing media or loading in serum-free media.

Baseline autofluorescence caused by components within growth media can be greatly reduced by the addition of the included Neuro Background Supressor. This solution has been specifically formulated for use with neuronal cells and will not cause osmotic shock. Additionally, the Neuro Background Suppressor has been used successfully with many different cell types to efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.

Propidium Iodide - FluoroPure™ Grade (Invitrogen™)

Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure grade propidium iodide is greater than or equal to 98% pure by HPLC.

SYTO™ 82 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.

Laurdan (6-Dodecanoyl-2-Dimethylaminonaphthalene) (Invitrogen™)

When the lipophlilic probe laurdan is incorporated into membranes, its fluorescence spectra is sensitive to the physical state of the surrounding phospholipids

DiIC18(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid) (Invitrogen™)

The far red fluorescent, lipophilic carbocyanine DiIC18(5)-DS contains sulfonate groups to improve water solubility. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. The sulfonate groups incorporated into this DiI analog improves water solubility. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

FM™ 5-95 (N-(3-Trimethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide) (Invitrogen™)

The increasing number of successful applications for our FM dyes has prompted us to synthesize FM 5-95, a slightly less lipophilic analog of FM 4-64 (T-3166, T-13320) with essentially identical spectroscopic properties.