Shop All Cell Stains

Ethidium Homodimer-1 (EthD-1) (Invitrogen™)

The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ~528/617).

Ethidium Homodimer-2 (EthD-2) - 1 mM Solution in DMSO (Invitrogen™)

The cell-impermeant viability indicator ethdium homodimer-2 (EthD-2) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ~535/624).

Dihydroethidium (Hydroethidine) (Invitrogen™)

The superoxide indicator dihydroethdium, also called hydroethidine, exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red.

DiIC18(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid) (Invitrogen™)

The far red fluorescent, lipophilic carbocyanine DiIC18(5)-DS contains sulfonate groups to improve water solubility. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. The sulfonate groups incorporated into this DiI analog improves water solubility. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

Rhodamine 123, 25 mg (Invitrogen™)

Rhodamine 123 is a cell-permeant, cationic, green-fluorescent dye that is readily sequestered by active mitochondria without cytotoxic effects. This product has been used to assay mitochondrial membrane potential in populations of apoptotic cells.

NucBlue™ Live ReadyProbes™ Reagent (Invitrogen™)

Hoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue® Live ReadyProbes® Reagent we have formulated this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells.

• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area
• Excited by UV light and emits blue fluorescence at 460 nm when bound to DNA nm

See other ReadyProbes® reagents for cell staining
See other nuclear stains for imaging

Cell imaging applications
The spectral properties of Hoechst 33342 (2'-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole), including a large Stokes shift, make it ideal for use with green (Alexa Fluor® 488, FITC, GFP) and red (Alexa Fluor® 594, Texas Red®, rhodamine, mCherry, mKate-2) fluorophores in multicolor experiments. Because of its high affinity to DNA, Hoechst 33342 is also frequently used in cell counting, cell cycle, and cell replication studies to distinguish condensed nuclei in apoptotic cells, for cell-cycle studies in combination with Click-iT® EdU or BrdU staining, as a nuclear segmentation tool in high content imaging analysis, and to sort cells based on their DNA content..

Suggestions for use
NucBlue® Live Cell Stain may be added directly to cells in full media, or buffer solutions.
• In most cases 2 drops/ml and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer, drops until the optimal staining intensity is obtained. In most cases, staining intensity increases with time if cells are not washed prior to imaging.
• NucBlue® Live Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter, blue GFP filters, or the Semrock BrightLine® Alexa Fluor® 350 Dye filter set.

DiR'; DiIC18(7) (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindotricarbocyanine Iodide) (Invitrogen™)

The near IR fluorescent, lipophilic carbocyanine DiOC18(7) ("DiR") is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. The sulfonate groups incorporated into this DiI analog improves water solubility. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

Hoechst 34580 (Invitrogen™)

This bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. Hoechst 34580 has somewhat longer-wavelength spectra than the other Hoechst dyes when bound to nucleic acids [Howard Shapiro, personal communication].

DiOC6(3) (3,3'-Dihexyloxacarbocyanine Iodide) (Invitrogen™)

DiOC6(3) is a cell-permeant, green-fluorescent, lipophilic dye that is selective for the mitochondria of live cells, when used at low concentrations. At higher concentrations, the dye may be used to stain other internal membranes, such as the endoplasmic reticulum.

NucBlue™ Fixed Cell ReadyProbes™ Reagent (Invitrogen™)

DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue® Fixed Cell ReadyProbes® Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells.

• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area• DAPI is excited with UV light and detected through a blue/cyan filter

See other ReadyProbes® reagents for cell staining
See other nuclear stains for imaging

Cell imaging applications
DAPI is a classic fluorescent dye used extensively for nuclear staining of fixed cells. In fluorescence microscopy, DAPI is excited with UV light and detected through a blue/cyan filter (Figure 1)..

Suggestions for use
In most cases 2 drops/ml of NucBlue® Fixed Cell Stain and an incubation of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more, or fewer, drops until the optimal staining intensity is obtained.
• NucBlue® Fixed Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter, blue GFP filters, or the Semrock BrightLine® Alexa Fluor® 350 Dye filter set.
• As a preferred blue nuclear stain in fixed cell imaging experiments, NucBlue® Fixed Cell Stain is ideal for use with antibody-based applications.

DiOC2(3) (3,3'-Diethyloxacarbocyanine Iodide) (Invitrogen™)

DiOC2(3) is a membrane potential probe. It has been used to analyze bacterial viability by flow cytometry using fluorescence emission ratio detection.

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) (Invitrogen™)

A popular nuclear and chromosome counterstain, DAPI emits blue fluorescence upon binding to AT regions of DNA. Although the dye is cell impermeant, higher concentrations will enter a live cell.

DASPEI (2-(4-(dimethylamino)styryl) -N-Ethylpyridinium Iodide) (Invitrogen™)

DASPEI is a styryl dye that stains mitochondria of live cells. This dye has a large fluorescence Stokes shift and is taken up relatively slowly as a function of membrane potential.

Hoechst 33258, Pentahydrate (bis-Benzimide) - FluoroPure™ Grade (Invitrogen™)

Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure Grade Hoechst 33258 is greater than or equal to 98% pure by HPLC.

DiO'; DiOC18(3) (3,3'-Dioctadecyloxacarbocyanine Perchlorate) (Invitrogen™)

The green fluorescent, lipophilic carbocyanine DiOC18(3) is widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.