Shop All Cell Stains

DiIC18(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid) (Invitrogen™)

The far red fluorescent, lipophilic carbocyanine DiIC18(5)-DS contains sulfonate groups to improve water solubility. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. The sulfonate groups incorporated into this DiI analog improves water solubility. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

Vybrant™ DyeCycle™ Violet Stain (Invitrogen™)

Vybrant® DyeCycle™ Violet Stain is a cell permeable DNA dye that can be used for cell cycle analysis and stem cell side population by flow cytometry.

• Precise—accurate cell cycle analysis in living cells

• Safe—low cytotoxicity for cell sorting and additional live cell experiments

• Minimal compensation—easier multiplexing

• Flexible

• Simple, robust staining protocol

View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.

Precise

Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant® DyeCycle™ Violet Stain is an ideal tool for DNA content analysis in living cells since the stain is cell permeable and, after binding double-stranded DNA, emits a fluorescent signal that is proportional to the DNA mass (see figure).

Low Cytotoxicity

Unlike stains that require high concentrations or have chemical structures that are toxic to cells, Vybrant® DyeCycle™ stains exhibit relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.

Minimal Compensation

Well-suited for the popular violet laser line, the Vybrant® DyeCycle™ Violet Stain/DNA complex has fluorescence excitation and emission maxima of 369/437 nm, respectively (see figure).

The violet excitation and narrow emission of Vybrant® DyeCycle™ Violet Stain make it ideal for multiplexing due to the limited spectral overlap with other common dyes (Alexa Fluor® 488, FITC, and RPE) and fluorescent proteins (Green Fluorescent Protein (GFP) and mCherry) . Vybrant® DyeCycle™ Violet Stain can also be used with UV excitation, having emission at ~440 nm.

Flexible

Vybrant® DyeCycle™ Violet Stain has been shown to not only work for both live cells and fixed cells in cell cycle assays, but to identify stem cell side populations and early progenitors in mammalian hematopoietic tissues (see figure).

Simple, Robust Staining Protocol

For cell analysis, simply prepare flow cytometry tubes each containing 1 mL of cells suspended in complete media at a cell concentration of 1 × 106 cells/mL. To each tube, add 1 µL of Vybrant® DyeCycle™ Violet Stain and mix well. Final stain concentration is 5 µM. Incubate at 37°C for 30 minutes, protected from light. Keep cells at 37°C until acquisition. Analyze samples without washing or fixing on a flow cytometer using ~405 nm excitation and ~440 nm emission.

DiR'; DiIC18(7) (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindotricarbocyanine Iodide) (Invitrogen™)

The near IR fluorescent, lipophilic carbocyanine DiOC18(7) ("DiR") is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. The sulfonate groups incorporated into this DiI analog improves water solubility. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

Vybrant™ DyeCycle™ Green Stain (Invitrogen™)

Vybrant® DyeCycle™ Green Stain is a cell-permeable DNA dye that can be used for cell cycle analysis on a 488 nm spectral line of the argon-ion laser, which is commonly used on all flow cytometers.

• Precise—accurate cell cycle analysis in living cells

• Safe—low cytotoxicity for cell sorting and additional live cell experiments

• Simple, robust staining protocol

View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.

Precise

Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant® DyeCycle™ Green Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).

Low Cytotoxicity

Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant® DyeCycle™ Green Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.

Simple, Robust Staining Protocol

For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 106 cells/mL. To each tube add 2 µL of Vybrant® DyeCycle™ Green Stain. Final stain concentration is 10 µM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on a flow cytometer using 488 nm excitation and green emission.

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) (Invitrogen™)

A popular nuclear and chromosome counterstain, DAPI emits blue fluorescence upon binding to AT regions of DNA. Although the dye is cell impermeant, higher concentrations will enter a live cell.

Vybrant™ DyeCycle™ Orange Stain (Invitrogen™)

Vybrant® DyeCycle™ Orange Stain is a cell permeable DNA dye that can be used for cell cycle analysis on 488 nm or 532 nm laser lines.

• Precise—accurate cell cycle analysis in living cells

• Safe—low cytotoxicity for cell sorting and additional live cell experiments

• Simple, robust staining protocol

View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.

Precise

Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant® DyeCycle™ Orange Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).

Low Cytotoxicity

Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant® DyeCycle™ Orange Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.

Simple, Robust Staining Protocol

For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 106 cells/mL. To each tube add 2 µL of Vybrant® DyeCycle™ Orange Stain. Final stain concentration is 10 µM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on flow cytometer using 488 nm excitation and orange emission.

DRAQ7™ Dye (Invitrogen™)

DRAQ7 Dye is a bright, easy-to-use fluorescent DNA dye used to exclude non-viable cells by flow cytometry. This dye has excitation maxima at 599/644 nm and emission maxima at 678/697 nm.

• Stable at room temperature
• Rapid staining of dead cells without wash steps
• No UV excitation and no emission spectral overlap with PE

DRAQ7 Dye is a membrane-impermeable dye that rapidly stains the double-stranded DNA (dsDNA) of dead or permeabilized cells. The dye can be excited by wavelengths from 488 to 647 nm, though it is optimally excited by red laser lines. Its emission maxima is at 697 nm when intercalated with dsDNA.

ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging (Invitrogen™)

ER-Tracker™ Green dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® FL dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K+ channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.

DiIC12(3) (1,1'-Didodecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate) (Invitrogen™)

The red-orange fluorescent, lipophilic carbocyanine DiIC12(3) is a shorter-chain DiI (DiIC18(3)) analog that may incorporate into membranes more easily than DiI. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

SYTO™ 12 Green Fluorescent Nucleic Acid Stain - 4 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 12 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 (S-7572) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

FAST DiO™ Solid; DiOΔ9,12-C18(3), ClO4 (3,3'-Dilinoleyloxacarbocyanine Perchlorate) (Invitrogen™)

FAST DiO is an unsaturated analog of DiO (DiOC18(3)) that reportedly migrates ~50% faster. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

eBioscience™ Indo-1 AM Calcium Sensor Dye (UltraPure Grade) (Invitrogen™)

Indo-1 AM Calcium Sensor Dye is a membrane-permeable dye used for determining changes in calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Once Indo-1 enters the cell, esterases cleave the AM group yielding a membrane-impermeable dye. Unbound Indo-1 has a peak emission at 475 nm. Upon binding calcium, the peak emission shifts down to 410 nm.

In flow cytometry this can be measured over time and can be represented as a ratio of the two emission wavelengths. To optimize the ratio between the two emissions, unbound Indo-1 fluorescence should be collected using a filter above 475nm (525 nm is a good option) while bound Indo-1 fluorescence should be collected using a filter below 400 nm. Because the emission profile of Indo-1 is broad, multicolor flow analysis using eFluor ™ 450, eVolve™ 605 or eVolve™ 655 is not possible, however fluorochrome conjugated antibodies utilizing the 488nm or 633 nm are quite compatible with Indo-1.

Molecular Weight: 1009.91
Peak Excitation: 346 nm
Peak Emission: 475 nm

Indo-1 AM should be reconstituted in high-quality, freshly opened DMSO. Recommended concentration: 1 mM.
Once reconstituted, it should be protected from light and stored at -20°C; avoid freeze-thaws.

Purity
>95% as determined by HPLC

Reported Application
Flow Cytometric Analysis, Microscopy, Immunohistochemical Staining

DRAQ7 DROP & GO™ Dye (Invitrogen™)

DRAQ7 DROP & GO Dye is a bright, easy-to-use fluorescent DNA dye used to exclude non-viable cells by flow cytometry. This dye has excitation maxima at 599/644 nm and emission maxima at 678/697 nm.

• Stable at room temperature
• Convenient, ready-to-use dropper-bottle format—no need to dilute, weigh, or pipette
• No UV excitation and no emission spectral overlap with PE

DRAQ7 Dye is a membrane-impermeable dye that rapidly stains the double-stranded DNA (dsDNA) of dead or permeabilized cells. The dye can be excited by wavelengths from 488 to 647 nm, though it is optimally excited by red laser lines. Its emission maxima is at 697 nm when intercalated with dsDNA.

SYTO™ 13 Green Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 13 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 (S-7572) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

MitoTracker™ Green FM - Special Packaging (Invitrogen™)

MitoTracker Green FM is green-fluorescent mitochondrial stain which appears to localize to mitochondria regardless of mitochondrial membrane potential. The dye will stain live cells but is not well-retained after aldehyde fixation.