Shop All Cell Stains

SYTOX™ AADvanced™ Dead Cell Stain Kit (Invitrogen™)

SYTOX™ AADvanced™ Dead Cell Stain (S10274, S10349) is a new high-affinity nucleic acid stain for the detection of dead cells and analysis of cell cycle using the common 488 nm blue laser in flow cytometry. The dye is spectrally similar to 7-AAD but with rapid kinetics of uptake and relatively low CVs. SYTOX™ AADvanced™ Dead Cell Stain penetrates cells more efficiently than 7-AAD providing better separation of live and dead cells. SYTOX™ AADvanced™ Dead Cell Stain can also be used with fixed cells for DNA content analysis when paired with RNAse treatment. SYTOX™ AADvanced™ Dead Cell Stain Kits contain a vial(s) of dried dye and anhydrous DMSO providing stable shelf life.

View a selection guide for all nonfixable viability dyes for flow cytometry.

Neutral Red, high purity (Invitrogen™)

The high-purity neutral red is a common lysosomal probe that stains lysosomes a fluorescent red.

Image-IT™ DEAD Green™ Viability Stain (Invitrogen™)

Image-iT® DEAD™ Green viability stain is a fast, sensitive and easy-to-use viability indicator that is also amenable to fixation and permeabilization, thus allowing for multiplexing with other biomarkers of cytotoxicity. Image-iT® DEAD™ Green is an impermeant dye to healthy cells that becomes permeant when the plasma membrane of cells is compromised.

SYTO™ 17 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 17 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.

FAST DiI™ solid; DiIΔ9,12-C18(3), CBS (1,1'-Dilinoleyl-3,3,3',3'-Tetramethylindocarbocyanine, 4-Chlorobenzenesulfonate) (Invitrogen™)

FAST DiI is an unsaturated analog of DiI (DiIC18(3)) that reportedly migrates ~50% faster. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.This is the solid form of D3899 to facilitate direct application of crystals to membranes.

NucBlue™ Live ReadyProbes™ Reagent (Invitrogen™)

Hoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue® Live ReadyProbes® Reagent we have formulated this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells.

• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area
• Excited by UV light and emits blue fluorescence at 460 nm when bound to DNA nm

See other ReadyProbes® reagents for cell staining
See other nuclear stains for imaging

Cell imaging applications
The spectral properties of Hoechst 33342 (2'-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole), including a large Stokes shift, make it ideal for use with green (Alexa Fluor® 488, FITC, GFP) and red (Alexa Fluor® 594, Texas Red®, rhodamine, mCherry, mKate-2) fluorophores in multicolor experiments. Because of its high affinity to DNA, Hoechst 33342 is also frequently used in cell counting, cell cycle, and cell replication studies to distinguish condensed nuclei in apoptotic cells, for cell-cycle studies in combination with Click-iT® EdU or BrdU staining, as a nuclear segmentation tool in high content imaging analysis, and to sort cells based on their DNA content..

Suggestions for use
NucBlue® Live Cell Stain may be added directly to cells in full media, or buffer solutions.
• In most cases 2 drops/ml and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer, drops until the optimal staining intensity is obtained. In most cases, staining intensity increases with time if cells are not washed prior to imaging.
• NucBlue® Live Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter, blue GFP filters, or the Semrock BrightLine® Alexa Fluor® 350 Dye filter set.

Vybrant™ DyeCycle™ Violet Ready Flow™ Reagent (Invitrogen™)

Vybrant DyeCycle Violet Ready Flow Reagent is a bright, easy-to-use, double-stranded DNA stain for cell cycle analysis in living cells using flow cytometry. It is excitable by a 405-nm laser with an emission peak at 437 nm.

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Accurate cell-cycle analysis in living cells
• Low toxicity for cell sorting and additional live cell experiments

Vybrant DyeCycle Violet Ready Flow Reagent is a ready-to-use, direct-to-sample DNA stain. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

As a cell-permeable dye, Vybrant DyeCycle Violet Ready Flow enters live cells and selectively binds dsDNA and emits a signal proportional to the DNA mass. The dye can be detected when excited with a 405-nm laser, and the emission captured at a peak of 437 nm. Vybrant DyeCycle Violet reagent is an ideal choice for DNA content analysis and can be included in multi-color cell cycle studies.

Lucifer Yellow CH, Potassium Salt (Invitrogen™)

Lucifer yellow CH, potassium salt is a water-soluble dye with excitation/emission peaks of 428/536 nm. It is a favorite tool for studying neuronal morphology, because it contains a carbohydrazide (CH) group that allows it to be covalently linked to surrounding biomolecules during aldehyde fixation.

HCS LipidTOX™ Green Neutral Lipid Stain, for cellular imaging (Invitrogen™)

The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Green neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid stains can also be used to monitor the formation and differentiation of adipocytes, a process called adipogenesis.

YO-PRO™-3 Iodide (612/631) - 1 mM Solution in DMSO (Invitrogen™)

One of our carbocyanine monomers, YO-PRO®-3 iodide is a useful counterstain and dead cell indicator, with excitation/emission maxima ~612/631 nm.

DiD' solid; DiIC18(5) solid (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt) (Invitrogen™)

The far-red fluorescent, lipophilic carbocyanine DiD is a longer-wavelength DiI analog. It is supplied as a solid form to facilitate direct application of crystals to membranes and is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

Hoechst 33258, Pentahydrate (bis-Benzimide), 100 mg (Invitrogen™)

Hoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. Its uses are similar to Hoechst 33342 (H-1399) for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant. This dye is also available in solution (H-3569).

NucBlue™ Fixed Cell ReadyProbes™ Reagent (Invitrogen™)

DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue® Fixed Cell ReadyProbes® Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells.

• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area• DAPI is excited with UV light and detected through a blue/cyan filter

See other ReadyProbes® reagents for cell staining
See other nuclear stains for imaging

Cell imaging applications
DAPI is a classic fluorescent dye used extensively for nuclear staining of fixed cells. In fluorescence microscopy, DAPI is excited with UV light and detected through a blue/cyan filter (Figure 1)..

Suggestions for use
In most cases 2 drops/ml of NucBlue® Fixed Cell Stain and an incubation of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more, or fewer, drops until the optimal staining intensity is obtained.
• NucBlue® Fixed Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter, blue GFP filters, or the Semrock BrightLine® Alexa Fluor® 350 Dye filter set.
• As a preferred blue nuclear stain in fixed cell imaging experiments, NucBlue® Fixed Cell Stain is ideal for use with antibody-based applications.

SYTO™ 12 Green Fluorescent Nucleic Acid Stain - 4 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 12 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 (S-7572) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

ER-Tracker™ Red (BODIPY™ TR Glibenclamide), for live-cell imaging (Invitrogen™)

ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K+ channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.