Shop All Other Cell Stains

RH 237 (N-(4-Sulfobutyl)-4-(6-(4-(Dibutylamino)phenyl)hexatrienyl)Pyridinium, Inner Salt) (Invitrogen™)

The fast-response potential-sensitive probe RH 237 can be used for imaging functional neurons.

NeuroTrace™ 530/615 Red Fluorescent Nissl Stain - Solution in DMSO (Invitrogen™)

Nissl staining is a standard histological method for visualizing neurons in the brain and spinal cord. Composed of ribosomal RNA associated with the rough endoplasmic reticulum in neuronal perikarya and dendrites, the Nissl substance redistributes within the cell body in injured or regenerating neurons, providing a marker for the physiological state of the neuron. Our NeuroTrace 530/615 red fluorescent Nissl stain is selective for the Nissl substance characteristic of neurons and provides more sensitivity than traditional histological dyes like toluidine blue or cresyl violet.

DiA; 4-Di-16-ASP (4-(4-(Dihexadecylamino)styryl)-N-Methylpyridinium Iodide) (Invitrogen™)

The lipophilic aminostyryl probe DiA inserts into membranes with its two alkyl tails and its fluorophore oriented parallel to the phospholipid acyl chain. When this dialkylaminostyryl probe binds to membranes, it exhibits a strong fluorescence enhancement; its fluorescence in water is minimal.

Fluorescein-5-(and-6)-Sulfonic Acid, Trisodium Salt (Invitrogen™)

The cell-impermeant fluorescein-5-(and-6)-sulfonic acid can be used as a non-fixable polar tracer to investigate fusion, lysis and gap-junctional communication.

FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Invitrogen™)

The styryl dye FM 4-64 has been reported to selectively stain yeast vacuolar membranes with red fluorescence (excitation/emission maxima ~515/640 nm). This lipophilic dye is an important tool for visualizing vacuolar organelle morphology and dynamics, for studying the endocytic pathway and for screening and characterizing yeast endocytosis mutants. FM 4-64 is also available as 1 mg in a single vial (T-3166).

eBioscience™ Fixable Viability Dye eFluor™ 450 (Invitrogen™)

Fixable Viability Dye eFluor™ 450 is a viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells. Thus, using Fixable Viability Dyes allows dead cells to be excluded from analysis when intracellular targets are being studied. Fixable Viability Dyes may be used to label cells from all species.

Fixable Viability Dye eFluor™ 450 can be excited by the violet (405 nm) laser line and has a peak excitation of 450 nm that can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™). Please make sure that your instrument is capable of detecting this dye. For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye. If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.

Fixable Viability Dye eFluor™ 450 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture. Store at -70°C with dessicant. It may be freeze-thawed up to 20 times. Allow vial to equilibrate to room temperature before opening.

Reported Application
Flow Cytometric Analysis

NeuroTrace™ 640/660 Deep-Red Fluorescent Nissl Stain - Solution in DMSO (Invitrogen™)

Nissl staining is a standard histological method for visualizing neurons in the brain and spinal cord. Composed of ribosomal RNA associated with the rough endoplasmic reticulum in neuronal perikarya and dendrites, the Nissl substance redistributes within the cell body in injured or regenerating neurons, providing a marker for the physiological state of the neuron. Our NeuroTrace 640/660 deep-red fluorescent Nissl stain is selective for the Nissl substance characteristic of neurons and provides more sensitivity than traditional histological dyes like toluidine blue or cresyl violet.

FAST DiO™ Solid; DiOΔ9,12-C18(3), ClO4 (3,3'-Dilinoleyloxacarbocyanine Perchlorate) (Invitrogen™)

FAST DiO is an unsaturated analog of DiO (DiOC18(3)) that reportedly migrates ~50% faster. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

alamarBlue™ Cell Viability Reagent (Invitrogen™)

alamarBlue Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability. Resazurin, the active ingredient of alamarBlue reagent, is a non-toxic, cell-permeable compound that is blue in color and virtually non-fluorescent. Upon entering living cells, resazurin is reduced to resorufin, a compound that is red in color and highly fluorescent. Changes in viability can be easily detected using either an absorbance- or fluorescence-based plate reader. alamarBlue Cell Viability Reagent has broad applicability and can be used with various human and animal cell lines, bacteria, plant, and fungi.

Features include:
• Robust and reliable performance—results in a large, highly reproducible dynamic range
• Highly sensitive reagent with a linear response—detects as few as 50 cells per well
• Convenient add-and-read format—no mixing, no washing, no cell lysis
• Compatible with either fluorescence- or absorbance-based instrumentation
• Measures viability from many diverse cell types—including mammalian cells, bacteria, plant, and fungi

Cell health can be monitored by numerous methods. Plasma membrane integrity, DNA synthesis, DNA content, enzyme activity, presence of ATP, and cellular reducing conditions are known indicators of cell viability and death. alamarBlue Cell Viability Reagent is an indigo-colored, non-toxic reagent that detects metabolically active cells and is used for the quantitative analysis of cell viability and proliferation. alamarBlue reagent has broad applicability and can be used with various human and animal cell lines, bacteria, plant, and fungi.

When added to cells, alamarBlue Cell Viability Reagent is modified by the reducing environment of viable cells and turns red in color and becomes highly fluorescent. This color change and increased fluorescence can be detected using absorbance (detected at 570 and 600 nm) or fluorescence (using an excitation between 530–560 and an emission at 590 nm). To assay for viability, simply add the pre-mixed alamarBlue reagent to cells in complete media (no wash or cell lysis steps required), incubate for one to four hours, and read using either an absorbance- or fluorescence-based plate reader. If necessary, longer incubation times may be used for greater sensitivity without compromising cell health.

The homogeneous, add-and-read assay format of the alamarBlue Cell Viability Reagent is fast, convenient, and readily amenable to automation and high-throughput assays. The color change and fluorescence increase resulting from cell viability changes allow the detection of alamarBlue reagent by either an absorbance- or fluorescence-based instrumentation.

eBioscience™ Fixable Viability Dye eFluor™ 660 (Invitrogen™)

Fixable Viability Dye eFluor™ 660 is a viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells. Thus, using Fixable Viability Dyes allows dead cells to be excluded from analysis when intracellular targets are being studied. These dyes may be used to label cells from all species.

Fixable Viability Dye eFluor™ 660 can be excited by the red (633 nm) laser line and has a peak emission of 660 nm that can be detected using a 660/20 band pass filter (equivalent to APC or Alexa Fluor™ 647). Please make sure that your instrument is capable of detecting this dye. For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye. If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.

Fixable Viability Dye eFluor™ 660 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture. Store at -70°C with dessicant. It may be freeze-thawed up to 20 times. Allow the vial to equilibrate to room temperature before opening.

Reported Application
Flow Cytometric Analysis

FAST DiI™ oil; DiIΔ9,12-C18(3), ClO4 (1,1'-Dilinoleyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate) (Invitrogen™)

FAST DiI is an unsaturated analog of DiI (DiIC18(3)) that reportedly migrates ~50% faster. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

eBioscience™ Fura-2 AM Dye (Invitrogen™)

Fura-2 is a calcium imaging dye that binds to free Ca2+. Fura-2 AM is the cell-permeable acetoxymethyl (AM) ester form of Fura-2. Fura-2 is the preferred dye for ratiometric imaging microscopy with digital image analysis, especially when the alteration of excitation wavelengths is more practical than the detection of multiple emission wavelengths. Upon binding Ca2+, the excitation spectrum of Fura-2 shifts to shorter wavelengths between 300 and 400 nm, while the peak emission remains steady around 510 nm. The Kd of Fura-2 is highly dependent on pH, temperature, ionic strength and viscosity of the cytosol, thus great care should be taken when non-standard conditions are used.

Molecular weight: 1001.86
Peak excitation: variable depending on the concentration of free Ca2+, between 300 and 400 nm
Peak emission: 510 nm

Fura-2 AM should be reconstituted in high-quality, freshly opened DMSO. Once reconstituted, it should be protected from light and stored at -20°C. Avoid freeze-thawing.

Reported Application
Microscopy, Cell Labeling

FM™ 5-95 (N-(3-Trimethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide) (Invitrogen™)

The increasing number of successful applications for our FM dyes has prompted us to synthesize FM 5-95, a slightly less lipophilic analog of FM 4-64 (T-3166, T-13320) with essentially identical spectroscopic properties.

FM™ 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide) (Invitrogen™)

FM 1-43 membrane probe is an excellent reagent both for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling. This water-soluble dye, which is nontoxic to cells and virtually nonfluorescent in aqueous medium, is believed to insert into the outer leaflet of the cell membrane where it becomes intensely fluorescent. In a neuron that is actively releasing neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and the nerve terminals become brightly stained. The nonspecific staining of cell-surface membranes can simply be washed off prior to viewing. FM 1-43 membrane probe is also available as 1 mg in a single vial (T-3163). FM 1-43FX membrane probe (F-35355), an analog of FM 1-43 membrane probe that can be fixed in place using aldehyde-based fixatives, is also available.

4-Di-10-ASP (4-(4-(Didecylamino)styryl)-N-Methylpyridinium Iodide) (Invitrogen™)

The lipophilic aminostyryl probe 4-Di-10-ASP contains a C10 alkyl chain and inserts into membranes with its two alkyl tails and its fluorophore orientted parallel to the phospholipid acyl chain. When this dialkylaminostyryl probe binds to membranes, it exhibits a strong fluorescence enhancement; its fluorescence in water is minimal.