Shop All Other Cell Stains

DiIC18(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid) (Invitrogen™)

The far red fluorescent, lipophilic carbocyanine DiIC18(5)-DS contains sulfonate groups to improve water solubility. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. The sulfonate groups incorporated into this DiI analog improves water solubility. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

Rhodamine 123, 25 mg (Invitrogen™)

Rhodamine 123 is a cell-permeant, cationic, green-fluorescent dye that is readily sequestered by active mitochondria without cytotoxic effects. This product has been used to assay mitochondrial membrane potential in populations of apoptotic cells.

DiR'; DiIC18(7) (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindotricarbocyanine Iodide) (Invitrogen™)

The near IR fluorescent, lipophilic carbocyanine DiOC18(7) ("DiR") is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. The sulfonate groups incorporated into this DiI analog improves water solubility. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

DiOC6(3) (3,3'-Dihexyloxacarbocyanine Iodide) (Invitrogen™)

DiOC6(3) is a cell-permeant, green-fluorescent, lipophilic dye that is selective for the mitochondria of live cells, when used at low concentrations. At higher concentrations, the dye may be used to stain other internal membranes, such as the endoplasmic reticulum.

DiOC2(3) (3,3'-Diethyloxacarbocyanine Iodide) (Invitrogen™)

DiOC2(3) is a membrane potential probe. It has been used to analyze bacterial viability by flow cytometry using fluorescence emission ratio detection.

DASPEI (2-(4-(dimethylamino)styryl) -N-Ethylpyridinium Iodide) (Invitrogen™)

DASPEI is a styryl dye that stains mitochondria of live cells. This dye has a large fluorescence Stokes shift and is taken up relatively slowly as a function of membrane potential.

DiO'; DiOC18(3) (3,3'-Dioctadecyloxacarbocyanine Perchlorate) (Invitrogen™)

The green fluorescent, lipophilic carbocyanine DiOC18(3) is widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

Nα-(3-Maleimidylpropionyl)Biocytin (Invitrogen™)

The thiol-reactive Na-(3-maleimidylpropionyl) biocytin can be used to attach biotin to biomolecules that can be subsequently detected with avidin or streptavidin conjugates.

HPTS; pyranine (8-Hydroxypyrene-1,3,6-Trisulfonic Acid, Trisodium Salt) (Invitrogen™)

8-Hydroxypyrene-1,3,6-trisulfonic acid (HPTS) is an inexpensive, highly water-soluble membrane-impermeant pH indicator with a pKa of ~7.3 in aqueous buffers.

DiIC18(3)-DS) 1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine-5,5'-Disulfonic Acid) (Invitrogen™)

The orange fluorescent, lipophilic carbocyanine DiIC18(3)-DS is a DiI analog that contains sulfonate groups to improve water solubility. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. The sulfonate groups incorporated into this DiI analog improves water solubility. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

Vybrant™ DiD Cell-Labeling Solution (Invitrogen™)

The Vybrant® DiD cell-labeling solution is a dye delivery solution that can be added directly to normal culture media to uniformly label suspended or attached culture cells for use in cell-cell fusion, cellular adhesion and migration applications.

eBioscience™ Calcein AM Viability Dye (UltraPure Grade) (Invitrogen™)

Calcein AM is a membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein. Calcein is optimally excited at 495 nm and has a peak emission of 515 nm. For fluorescent microscopy, Calcein can be detected using the appropriate filter sets. For flow cytometric analysis, it can be excited off the blue laser line (488 nm) and detected using filters for FITC/Alexa Fluor™ 488 (530/30). For multicolor flow cytometry, concentrations in the range of 5 – 20 nM are recommended to ensure that compensation can be performed successfully. Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells. If single-color analysis is desired, improved resolution between live and dead/apoptotic cells may be obtained by using either Calcein Blue AM (cat. 65-0855) or Calcein Violet 450 AM (cat. 65-0854).

Molecular weight: 994.86
Peak excitation: 495 nm
Peak emission: 515 nm

Calcein AM should be reconstituted in high-quality, freshly opened DMSO.
Once reconstituted, it should be stored at -20°C with dessicant and used within a short period of time. Avoid freeze-thawing.

Purity
>95% as determined by HPLC.

Reported Application
Flow Cytometric Analysis, Microscopy

eBioscience™ Fixable Viability Dye eFluor™ 450 (Invitrogen™)

Fixable Viability Dye eFluor™ 450 is a viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells. Thus, using Fixable Viability Dyes allows dead cells to be excluded from analysis when intracellular targets are being studied. Fixable Viability Dyes may be used to label cells from all species.

Fixable Viability Dye eFluor™ 450 can be excited by the violet (405 nm) laser line and has a peak excitation of 450 nm that can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™). Please make sure that your instrument is capable of detecting this dye. For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye. If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.

Fixable Viability Dye eFluor™ 450 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture. Store at -70°C with dessicant. It may be freeze-thawed up to 20 times. Allow vial to equilibrate to room temperature before opening.

Reported Application
Flow Cytometric Analysis

DRAQ7™ Dye (Invitrogen™)

DRAQ7 Dye is a bright, easy-to-use fluorescent DNA dye used to exclude non-viable cells by flow cytometry. This dye has excitation maxima at 599/644 nm and emission maxima at 678/697 nm.

• Stable at room temperature
• Rapid staining of dead cells without wash steps
• No UV excitation and no emission spectral overlap with PE

DRAQ7 Dye is a membrane-impermeable dye that rapidly stains the double-stranded DNA (dsDNA) of dead or permeabilized cells. The dye can be excited by wavelengths from 488 to 647 nm, though it is optimally excited by red laser lines. Its emission maxima is at 697 nm when intercalated with dsDNA.

FM™ 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide) (Invitrogen™)

FM 1-43 membrane probe is an excellent reagent both for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling. This water-soluble dye, which is nontoxic to cells and virtually nonfluorescent in aqueous medium, is believed to insert into the outer leaflet of the cell membrane where it becomes intensely fluorescent. In a neuron that is actively releasing neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and the nerve terminals become brightly stained. The nonspecific staining of cell-surface membranes can simply be washed off prior to viewing. FM 1-43 membrane probe is also available as 1 mg packaged into 10 vials, each containing 100 µg (T-35356). FM 1-43FX membrane probe (F-35355), an analog of FM 1-43 membrane probe that can be fixed in place using aldehyde-based fixatives, is also available.