Shop All Other Cell Stains

Dil Stain (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate ('DiI'; DiIC18(3))) Invitrogen™

DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solution (V-22885), as a paste (N-22880) or as a solid (D-282).

4-Di-1-ASP (4-(4-(Dimethylamino)styryl) -N-Methylpyridinium Iodide) Invitrogen™

DASPMI (4-Di-1-ASP) is a styryl dye stains mitochondria of live cells. This dye has a large fluorescence Stokes shift and is taken up relatively slowly as a function of membrane potential.

FM™ 4-64FX, fixable analog of FM™ 4-64 membrane stain Invitrogen™

The lipophilic probe, FM™ 4-64FX exhibits low fluorescence in water but fluoresces intensely upon binding the outer leaf of the plasma membrane providing discrete plasma membrane staining. The binding is rapid and reversible and 'FX' denotes that this analog is fixable with aldehyde-based fixatives. It is also an excellent reagent for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling.

FM™ 5-95 (N-(3-Trimethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide) Invitrogen™

The increasing number of successful applications for our FM dyes has prompted us to synthesize FM 5-95, a slightly less lipophilic analog of FM 4-64 (T-3166, T-13320) with essentially identical spectroscopic properties.

Alkaline Phosphatase Live Stain Invitrogen™

Alkaline Phosphatase Live Stain is a stem cell imaging product that allows users to differentially stain pluripotent stem cells (PSCs). The dye is a cell-permeable fluorescent substrate for alkaline phosphatase (AP) that is non-toxic to cells, diffusing out over the course of two hours. The Alkaline Phosphatase Live Stain consists of 50 µL of a 500X fluorescein-based dye in DMSO, sufficient for staining two 24-well plates, four 6 cm dishes or two 10 cm dishes.

This Alkaline Phosphatase Live Stain allows you to:

• Identify pluripotent stem cells with a strong signal above background
• Stain PSCs typically in 20 minutes or less
• Use the first alkaline phosphatase stain marketed that maintains cell viability

Alkaline Phosphatase Pluripotent Stem Cell Detection Method
AP is a phenotypic marker of PSCs, including undifferentiated embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and embryonic germ cells (EGCs). While AP is expressed in most cell types, its expression is highly elevated in PSCs. AP staining has therefore been used to differentially stain PSCs to easily distinguish them from mouse embryonic fibroblasts (MEFs) used as feeders and parental fibroblasts commonly used in reprogramming experiments.

Screen for Pluripotency Without Sacrificing Precious Colonies
The Alkaline Phosphatase Live Stain is ideal for screening colonies during early stages of the reprogramming workflow since the stain maintains stem cell viability. It can also be used in later stages as a negative selection tool for identifying undifferentiated cells.

Stain Cells Quickly
The Alkaline Phosphatase Live Stain utilizes an easy, non-permanent, cell-viable protocol for identifying PSCs in your experiments. Simply dilute the dye in media, apply to cells, gently wash, and the cells are ready for fluorescent imaging.

For Research Use Only. Not for use in diagnostics procedures.

FM™ 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide) Invitrogen™

FM 1-43 membrane probe is an excellent reagent both for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling. This water-soluble dye, which is nontoxic to cells and virtually nonfluorescent in aqueous medium, is believed to insert into the outer leaflet of the cell membrane where it becomes intensely fluorescent. In a neuron that is actively releasing neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and the nerve terminals become brightly stained. The nonspecific staining of cell-surface membranes can simply be washed off prior to viewing. FM 1-43 membrane probe is also available as 1 mg in a single vial (T-3163). FM 1-43FX membrane probe (F-35355), an analog of FM 1-43 membrane probe that can be fixed in place using aldehyde-based fixatives, is also available.

Tetramethylrhodamine, Methyl Ester, Perchlorate (TMRM) Invitrogen™

Tetramethylrhodamine, methyl ester (TMRM) is a cell-permeant, cationic, red-orange fluorescent dye that is readily sequestered by active mitochondria.

4-Di-10-ASP (4-(4-(Didecylamino)styryl)-N-Methylpyridinium Iodide) Invitrogen™

The lipophilic aminostyryl probe 4-Di-10-ASP contains a C10 alkyl chain and inserts into membranes with its two alkyl tails and its fluorophore orientted parallel to the phospholipid acyl chain. When this dialkylaminostyryl probe binds to membranes, it exhibits a strong fluorescence enhancement; its fluorescence in water is minimal.

Di-8-ANEPPS Invitrogen™

ANEP dyes are molecules that fluoresce in response to electrical potential changes in their environment. These are fast-response probes that operate by means of a change in their electronic structure, and consequently their fluorescence properties, in response to a change in the surrounding electric field. Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells, and intact brains. However, the magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show a 2-10% fluorescence change per 100 mV. Furthermore, these dyes display a potential-dependent shift in their excitation spectra, thus permitting the quantitation of membrane potential using excitation ratio measurements.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Potential-Sensitive ANEP Dye Specifications:
• Zwitterionic molecule; is less susceptible to internalization than di-4-ANEPPS, permitting extended observation
• Ex/Em maxima bound to model phospholipid membranes are ~465/635 nm (but spectral properties are highly dependent on environment)
• Nonfluorescent until bound to membranes
• Soluble in ethanol, DMSO, and DMF (di-2-ANEPEQ is a water-soluble ANEP dye)
• Dye is introduced into cells by direct addition of stock solution to cell culture medium, by using Pluronic® F-127, or by retrograde labeling
• Fast-response probe, suitable for detecting submillisecond membrane potential changes

Applications for Potentiometric Probes
The plasma membrane of a cell typically has a transmembrane potential of approximately -70 mV (negative inside) as a consequence of K+, Na+, and Cl- concentration gradients that are maintained by active transport processes. Potentiometric probes offer an indirect method of detecting the translocation of these ions.

Increases and decreases in membrane potential-referred to as membrane hyperpolarization and depolarization, respectively-play a central role in many physiological processes, including nerve-impulse propagation, muscle contraction, cell signaling and ion-channel gating. Potentiometric probes are important tools for studying these processes.

Find More ANEP Dyes
We offer ANEP dyes in various forms. Review Fast-Response Probes-Section 22.2 in the Molecular Probes® Handbook for more information on these probes.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

HPTS; pyranine (8-Hydroxypyrene-1,3,6-Trisulfonic Acid, Trisodium Salt) Invitrogen™

8-Hydroxypyrene-1,3,6-trisulfonic acid (HPTS) is an inexpensive, highly water-soluble membrane-impermeant pH indicator with a pKa of ~7.3 in aqueous buffers.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Rhodamine 123, FluoroPure™ grade Invitrogen™

Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure grade rhodamine 123 is greater than or equal to 98% pure by HPLC.

ACMA (9-Amino-6-Chloro-2-Methoxyacridine) Invitrogen™

ACMA (9-amino-6-chloro-2-methoxyacridine) is a DNA intercalator that selective binds to poly (d(A-T)). Excitation of the ACMA-DNA complex (excitation/emission maxima ~419/483 nm) is possible with most UV-light sources, making it compatible for use with both shorter- and longer-wavelength dyes. ACMA also apparently binds to membranes in the energized state and becomes quenched if a pH gradient forms. It has been extensively employed to follow cation and anion movement across membranes and to study the proton-pumping activity of various membrane-bound ATPases.

alamarBlue™ Cell Viability Reagent Invitrogen™

alamarBlue Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability. Resazurin, the active ingredient of alamarBlue reagent, is a non-toxic, cell-permeable compound that is blue in color and virtually non-fluorescent. Upon entering living cells, resazurin is reduced to resorufin, a compound that is red in color and highly fluorescent. Changes in viability can be easily detected using either an absorbance- or fluorescence-based plate reader. alamarBlue Cell Viability Reagent has broad applicability and can be used with various human and animal cell lines, bacteria, plant, and fungi.

Features include:
• Robust and reliable performance—results in a large, highly reproducible dynamic range
• Highly sensitive reagent with a linear response—detects as few as 50 cells per well
• Convenient add-and-read format—no mixing, no washing, no cell lysis
• Compatible with either fluorescence- or absorbance-based instrumentation
• Measures viability from many diverse cell types—including mammalian cells, bacteria, plant, and fungi

Cell health can be monitored by numerous methods. Plasma membrane integrity, DNA synthesis, DNA content, enzyme activity, presence of ATP, and cellular reducing conditions are known indicators of cell viability and death. alamarBlue Cell Viability Reagent is an indigo-colored, non-toxic reagent that detects metabolically active cells and is used for the quantitative analysis of cell viability and proliferation. alamarBlue reagent has broad applicability and can be used with various human and animal cell lines, bacteria, plant, and fungi.

When added to cells, alamarBlue Cell Viability Reagent is modified by the reducing environment of viable cells and turns red in color and becomes highly fluorescent. This color change and increased fluorescence can be detected using absorbance (detected at 570 and 600 nm) or fluorescence (using an excitation between 530–560 and an emission at 590 nm). To assay for viability, simply add the pre-mixed alamarBlue reagent to cells in complete media (no wash or cell lysis steps required), incubate for one to four hours, and read using either an absorbance- or fluorescence-based plate reader. If necessary, longer incubation times may be used for greater sensitivity without compromising cell health.

The homogeneous, add-and-read assay format of the alamarBlue Cell Viability Reagent is fast, convenient, and readily amenable to automation and high-throughput assays. The color change and fluorescence increase resulting from cell viability changes allow the detection of alamarBlue reagent by either an absorbance- or fluorescence-based instrumentation.

NeuroTrace™ DiI Tissue-Labeling Paste Invitrogen™

NeuroTrace tissue labeling pastes consist of DiI, DiO (N-22881) or DiD (N-22882) mixed into an inert, water-resistant gel. The pastes are ready to use as supplied and can be applied directly to live or fixed tissue specimens using the tip of a needle.

NeuroTrace™ BDA-10,000 Neuronal Tracer Kit Invitrogen™

The NeuroTrace™ BDA-10,000 Neuronal Tracer Kit contains the neuronal tracer, biotin dextran amine (BDA), and the detection reagents, avidin-horseradish peroxidase (avidin-HRP) and diaminobenzedine (DAB) for subsequent visualization by light or electron microscopy.
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