Shop All Organelle Stains

Image-iT™ TMRM Reagent (mitochondrial membrane potential indicator) (Invitrogen™)

Healthy mitochondrial membranes maintain a difference in electrical potential between the interior and exterior of the organelle, referred to as a membrane potential. Tetramethylrhodamine, methyl ester (TMRM) is a cell-permeant dye that accumulates in active mitochondria with intact membrane potentials. If the cells are healthy and have functioning mitochondria, the signal will be bright. Upon loss of the mitochondrial membrane potential, TMRM accumulation will cease and the signal will dim or disappear. TMRM signal can be detected with fluorescence microscopy, flow cytometry, cell sorting, high-throughput screening, and high content analysis.

• For live-cell dynamic study of mitochondrial membrane potential (not fixable)
• Detectable with RFP/TRITC filter set (absorbance peak: 548 nm, emission peak: 574 nm)
• Mutiplexable with green, deep red, violet, and blue emission dyes, such as GFP, DAPI, Hoechst, HCS LipidTOX, and LysoTracker Deep Red

LysoSensor™ Yellow/Blue dextran, 10,000 MW, Anionic, Fixable (Invitrogen™)

LysoSensor Yellow/Blue dextran is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. This dextran conjugate is taken up by the cells through endocytosis. The LysoSensor dye produces blue fluorescence in neutral environments, but changes to yellow fluorescence in more acidic environments. The pKa of LysoSensor Yellow/Blue dextran is ~3.9.

MitoTracker™ Orange CMTMRos - Special Packaging (Invitrogen™)

MitoTracker® Orange CMTMRos is an orange-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.

Yeast Mitochondrial Stain Sampler Kit (Invitrogen™)

The Yeast Mitochondrial Stain Sampler Kit contains sample quantities of five different cell-permeant probes that have been found to label mitochondria in live yeast. In addition to the well characterized mitochondrial stains rhodamine 123, and DiOC6(3), this kit includes rhodamine B hexyl ester, MitoTracker® Green FM and SYTO® 18 yeast mitochondrial stain, a proprietary green fluorescent nucleic acid stain. Each of these probes is sequestered in functioning mitochondria.

LysoTracker™ Red DND-99 - Special Packaging (Invitrogen™)

LysoTracker® Red DND-99 is a red-fluorescent dye for labeling and tracking acidic organelles in live cells.

Features of all LysoTracker® probes include:

 High selectivity for acidic organelles
•  Good retention after aldehyde fixation
•  Effective labeling of live cells at nanomolar concentrations
•  Live cell imaging
•  Excitation/Emission: 577/590 nm

Simple, Highly Specific, One-Step Staining and Tracking of Acidic Organelles
The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This allows LysoTracker® probes to freely permeate cell membranes enabling them to label live cells. LysoTracker® probes are highly selective for acidic organelles, and unlike dinitrophenyl (DNP), LysoTracker® probes do not require use of a second antibody for detection.

LysoTracker® probes are available in a variety of fluorescent colors.

Related Links

•  See characteristics of all LysoTracker and LysoSensor Probes.
•  See other Molecular Probes Fluorescent Organelle Stains.
•  Create a Cellular Masterpiece with LysoTracker® Red DND-99 using the Cell Paint Tool.

CellMask™ Orange Plasma membrane Stain (Invitrogen™)

The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The stain survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies.

MitoTracker™ Orange CM-H2TMRos - Special Packaging (Invitrogen™)

MitoTracker® Orange CM-H2TMRos is a reduced, nonfluorescent version of MitoTracker Orange (M-7510) that fluoresces upon oxidation. This dye also stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.

Yeast Vacuole Marker Sampler Kit (Invitrogen™)

The Yeast Vacuole Marker Sampler Kit contains sample quantities of a series of both novel and well established vacuole marker probes for the study of yeast cell biology. There are two blue-fluorescent coumarin-based stains that selectively stain the lumen of the yeast vacuole. There is also a green-fluorescent yeast vacuole membrane marker MDY-64 for staining the vacuole membrane. Finally, this kit also includes the commonly used vaculate marker 5-(and-6)-carboxy-2',7-dicholorofluorescein diacetate (carboxy-DCFDA) as a standard.

LysoTracker™ Deep Red (Invitrogen™)

LysoTracker® Deep Red is a deep red-fluorescent dye for labeling and tracking acidic organelles in live cells.

Features of all LysoTracker® probes include:

• High selectivity for acidic organelles
• Effective labeling of live cells at nanomolar concentrations
• Live-cell imaging
• With excitation/emission of 647/668 nm, the same filter set can be used as used for Cy5 or MitoTracker® Deep Red

Simple, Highly Specific, One-Step Staining and Tracking of Acidic Organelles
The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This allows LysoTracker® probes to freely permeate cell membranes enabling them to label live cells. LysoTracker® probes are highly selective for acidic organelles, and unlike dinitrophenyl (DNP), LysoTracker® probes do not require use of a second antibody for detection.

LysoTracker® probes are available in a variety of fluorescent colors.

LysoSensor™ Blue DND-167 - Special Packaging (Invitrogen™)

LysoSensor Blue DND-167 can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye becomes more fluorescent in acidic environments. The pKa of LysoSensor Blue DND-167 is ~5.1.

CellMask™ Deep Red Plasma membrane Stain (Invitrogen™)

The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The stain survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies.

MitoTracker™ Red CMXRos - Special Packaging (Invitrogen™)

MitoTracker Red CMXRos is a red-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.

Yeast Vacuole Membrane Marker MDY-64 (Invitrogen™)

MDY-64 is a green-fluorescent yeast vacuole membrane stain.

LysoTracker™ Blue DND-22 - Special Packaging (Invitrogen™)

LysoTracker® Blue DND-22 is a blue fluorescent dye that stains acidic compartments in live cells with excitation/emission maxima ~373/422 nm.

LysoSensor™ Green DND-189 - Special Packaging (Invitrogen™)

LysoSensor™ Green DND-189 can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye becomes more fluorescent in acidic environments. The pKa of LysoSensor™ Green DND-189 is ~5.2.