Shop All Organelle Stains

ER-Tracker™ Blue-White DPX, for live-cell imaging (Invitrogen™)

ER-Tracker Blue-White DPX dye is a photostable probe that is selective for the endoplasmic reticulum (ER) in live cells. The cells can be aldehyde-fixed after staining with the dye but significant fluorescence signal will be lost. With excitation at ~374 nm, the environmentally-sensitive probe exhibits emission between 430 and 640 nm. A long-pass DAPI filter works best.

LysoTracker™ Deep Red (Invitrogen™)

LysoTracker® Deep Red is a deep red-fluorescent dye for labeling and tracking acidic organelles in live cells.

Features of all LysoTracker® probes include:

• High selectivity for acidic organelles
• Effective labeling of live cells at nanomolar concentrations
• Live-cell imaging
• With excitation/emission of 647/668 nm, the same filter set can be used as used for Cy5 or MitoTracker® Deep Red

Simple, Highly Specific, One-Step Staining and Tracking of Acidic Organelles
The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This allows LysoTracker® probes to freely permeate cell membranes enabling them to label live cells. LysoTracker® probes are highly selective for acidic organelles, and unlike dinitrophenyl (DNP), LysoTracker® probes do not require use of a second antibody for detection.

LysoTracker® probes are available in a variety of fluorescent colors.

Image-IT™ LIVE Mitochondrial Transition Pore Assay Kit, for microscopy (Invitrogen™)

The Image-iT® LIVE Mitochondrial Transition Pore Assay Kit provides a proven method[REF PN36389 ,PN32679] of measuring mitochondrial permeability transition pore opening. The kit includes a dye and a quencher that are easily loaded into cells. In healthy cells, the dye, but not the quencher, enters mitochondria. The mitochondria remain brightly fluorescent until mitochondrial pore activation permits quenching of the fluorescence.

CellMask™ Orange Plasma membrane Stain (Invitrogen™)

The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The stain survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies.

CellMask™ Green Plasma Membrane Stain (Invitrogen™)

The CellMask™ Green Plasma Membrane Stain enables fast and uniform labeling of the plasma membrane in almost all cell types. This stain works rapidly and can be detected using a standard FITC filter set with any imaging instrument. Depending upon the cell type and experimental conditions, the plasma membrane staining will last for 60–90 minutes without detectable internalization, providing enough time for most live cell dynamic studies. The stain survives fixation, but not permeabilization.

Features of the CellMask™ Green Plasma Membrane Stain include:

• Sensitivity—specific and rapid plasma membrane staining
• Excellent retention—staining lasts for 60–90 minutes
• Emission/excitation (522/535 nm) works with standard FITC settings

Drawbacks of Other Methods
The plasma membrane is a convenient marker of cell boundaries and as such, a number of probes have been used for staining of the membrane. Typically, dyes of a lipophilic nature are used; however, they internalize rapidly, offering a very narrow window for imaging. Fluorescently labeled lectins, such as wheat germ agglutinin, have also been employed as plasma membrane stains. Conjugated lectins depend on cell surface sugars for staining and as a result, stain inconsistently with variation across cell types. Robust plasma membrane staining is important for a range of applications including translocation assays, plasma membrane dynamics, and as a general tool for cell identification in traditional and automated imaging and analysis.

How CellMask™ Plasma Membrane Stains Work
CellMask™ Green Plasma Membrane Stain is designed to deliver uniform staining of the plasma membrane across a wide variety of mammalian cell types and is slow to internalize, particularly compared to traditional approaches such as DiI, DiO, and labeled wheat germ agglutinin. CellMask™ plasma membrane stains are amphipathic molecules containing a lipophilic moiety for excellent membrane loading and a negatively charged hydrophilic dye for "anchoring" of the probe in the plasma membrane. While the CellMask™ plasma membrane stains provide ample opportunity for live cell imaging, the staining pattern is also maintained after fixation with formaldehyde, enabling additional multiparametric imaging options. However, staining with CellMask™ plasma membrane stains does not survive detergent extraction and therefore cannot be used in conjunction with probes that require permeabilization.

Yeast Vacuole Membrane Marker MDY-64 (Invitrogen™)

MDY-64 is a green-fluorescent yeast vacuole membrane stain.

Yeast Mitochondrial Stain Sampler Kit (Invitrogen™)

The Yeast Mitochondrial Stain Sampler Kit contains sample quantities of five different cell-permeant probes that have been found to label mitochondria in live yeast. In addition to the well characterized mitochondrial stains rhodamine 123, and DiOC6(3), this kit includes rhodamine B hexyl ester, MitoTracker® Green FM and SYTO® 18 yeast mitochondrial stain, a proprietary green fluorescent nucleic acid stain. Each of these probes is sequestered in functioning mitochondria.

Image-iT™ TMRM Reagent (mitochondrial membrane potential indicator) (Invitrogen™)

Healthy mitochondrial membranes maintain a difference in electrical potential between the interior and exterior of the organelle, referred to as a membrane potential. Tetramethylrhodamine, methyl ester (TMRM) is a cell-permeant dye that accumulates in active mitochondria with intact membrane potentials. If the cells are healthy and have functioning mitochondria, the signal will be bright. Upon loss of the mitochondrial membrane potential, TMRM accumulation will cease and the signal will dim or disappear. TMRM signal can be detected with fluorescence microscopy, flow cytometry, cell sorting, high-throughput screening, and high content analysis.

• For live-cell dynamic study of mitochondrial membrane potential (not fixable)
• Detectable with RFP/TRITC filter set (absorbance peak: 548 nm, emission peak: 574 nm)
• Mutiplexable with green, deep red, violet, and blue emission dyes, such as GFP, DAPI, Hoechst, HCS LipidTOX, and LysoTracker Deep Red

CellMask™ Deep Red Plasma membrane Stain (Invitrogen™)

The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The stain survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies.

ER-Tracker™ Red (BODIPY™ TR Glibenclamide), for live-cell imaging (Invitrogen™)

ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K+ channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.

MitoTracker™ Green FM - Special Packaging (Invitrogen™)

MitoTracker Green FM is green-fluorescent mitochondrial stain which appears to localize to mitochondria regardless of mitochondrial membrane potential. The dye will stain live cells but is not well-retained after aldehyde fixation.

MitoTracker™ Orange CMTMRos - Special Packaging (Invitrogen™)

MitoTracker® Orange CMTMRos is an orange-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.

LysoSensor™ Green DND-189 - Special Packaging (Invitrogen™)

LysoSensor™ Green DND-189 can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye becomes more fluorescent in acidic environments. The pKa of LysoSensor™ Green DND-189 is ~5.2.

LysoTracker™ Yellow HCK-123 - Special Packaging (Invitrogen™)

LysoTracker® Yellow HCK-123 is a yellow fluorescent dye that stains acidic compartments in live cells with excitation/emission maxima ~465/535 nm.

Yeast Vacuole Marker Sampler Kit (Invitrogen™)

The Yeast Vacuole Marker Sampler Kit contains sample quantities of a series of both novel and well established vacuole marker probes for the study of yeast cell biology. There are two blue-fluorescent coumarin-based stains that selectively stain the lumen of the yeast vacuole. There is also a green-fluorescent yeast vacuole membrane marker MDY-64 for staining the vacuole membrane. Finally, this kit also includes the commonly used vaculate marker 5-(and-6)-carboxy-2',7-dicholorofluorescein diacetate (carboxy-DCFDA) as a standard.