Shop All DNA⁄RNA Cell Stains

TO-PRO™3 Ready Flow™ Reagent (Invitrogen™)

TO-PRO™3 Ready Flow™ Reagent is a bright and easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. This dye has excitation/emission maxima of 642/661 nm, similar to Alexa Fluor™ 647 or allophycocyanin (APC).

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Rapid staining of dead cells without wash steps

TO-PRO 3 Ready Flow Reagent is a ready-to-use, direct-to-sample dead-cell stain that emits red fluorescence when bound to DNA. Add 2 drops form the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

TO-PRO 3 Ready Flow Reagent is membrane impermeant and therefore excluded from viable cells. It binds selectively to double-stranded DNA. This dye has excitation/emission maxima of 642/661 nm.

SYTO™ Orange Fluorescent Nucleic Acid Stain Sampler Kit - SYTO™ dyes 80-85 - 50 µL each (Invitrogen™)

The SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit contains our collection of cell-permeant, orange-fluorescent SYTO nucleic acid stains. Because the dyes may demonstrate different staining behaviors with various tissues and cells, it may be necessary to test the dyes to find the optimal dye for a specific application. The kit contains 50 µL each of SYTO 80—85 dyes.

SYTO™ 11 Green Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 11 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 (S-7572) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

TOTO™-1 Iodide (514/533) - 1 mM Solution in DMSO (Invitrogen™)

TOTO®-1 iodide is a cell-impermeant, high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~514/533 nm when bound to nucleic acids.

Hoechst 33258, Pentahydrate (bis-Benzimide) - FluoroPure™ Grade (Invitrogen™)

Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure Grade Hoechst 33258 is greater than or equal to 98% pure by HPLC.

SYTO™ 82 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.

SYTO™ 12 Green Fluorescent Nucleic Acid Stain - 4 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 12 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 (S-7572) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

DAPI (4',6-Diamidino-2-Phenylindole, Dilactate) (Invitrogen™)

DAPI, dilactate is a water-soluble form of the popular nuclear and chromosome counterstain, DAPI. The nuclear stain emits blue fluorescence upon binding to AT regions of DNA. Although the dye is cell impermeant, higher concentrations will enter a living cell.

TO-PRO™-1 Iodide (515/531) - 1 mM Solution in DMSO (Invitrogen™)

One of our carbocyanine monomers, TO-PRO®-1 iodide is a useful counterstain and dead cell indicator, with excitation/emission maxima ~515/531 nm.

Propidium Iodide - 1.0 mg/mL Solution in Water (Invitrogen™)

Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.

PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ~30–40 nm to the red and the fluorescence emission maximum is shifted ~15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm.

PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry.

Learn more about propidium iodide, and propidium iodide containing products

SYTO™ 64 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO (Invitrogen™)

The cell-permeant SYTO 64 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.

JO-PRO™-1 Iodide (530/546) - 1 mM Solution in DMSO (Invitrogen™)

JO-PRO-1 iodide is a cell-impermeant nucleic acid stain that exhibits bright, orange fluorescence (~545 nm) upon binding to nucleic acids.

SYTOX™ Green Dead Cell Stain, for flow cytometry (Invitrogen™)

SYTOX™ Green Dead Cell Stain is a bright, easy-to-use nucleic acid stain for distinguishing dead from live cells in flow cytometry assays. It is excitable at 488 nm with an emission profile similar to FITC, and has been tested in an assortment of cell types.

View a selection guide for all nonfixable viability dyes for flow cytometry.

You get these advantages with SYTOX™ Green Dead Cell Stain:
•  Bright Signal—Easily distinguishes dead cells from live cells.
•  Rapid Staining—Single step, No Wash Assay to assess viability of unfixed cells.
•  Validation—in Jurkat, ReH, CHO, Kg1a, 3T3, HeLa, HEK (primary cells), and lysed whole blood.
•  Optimization—Dye concentration has been optimized for use in flow cytometry.
•  Versatility—Tested in a variety of experimental applications.

A bright stain in an easy to use, no wash assay
SYTOX™ Green Dead Cell Stain is a cell-impermeant nucleic acid stain. By flow cytometry, dead cells can be easily distinguished from live cells by the increased fluorescent signal due to dsDNA binding of SYTOX™ Green (Fig 1). Because it is non-fluorescent in aqueous media, SYTOX™ Green Dead Cell Stain can be applied to cells and visualized with minimal background staining and without an additional wash step.

Validated in flow cytometry
SYTOX™ Green is designed for flow cytometry using the 488 nm blue laser with emission in the 530/30 BP filter (similar to FITC and GFP). The dye concentration has been optimized for samples with concentrations ranging from 1x104 to 1x106 cells per ml.

SYTOX™ Green Cell Stain has been validated in several cell types including:
•  Jurkat
•  ReH
•  CHO
•  Kg1a
•  3T3
•  HeLa
•  HEK (primary cells)
•  Lysed whole blood

SYTOX™ Green Dead Cell Stain has been tested in several applications including:
•  Apoptosis (Fig 2)
•  Cell cycle
•  Cell proliferation
•  Immunophenotyping assays

Part of the SYTOX™ family of cell viability stains
SYTOX™ Dead Cell Stains come in an assortment of excitation/emission characteristics and have been incorporated into a number of assays for apoptosis, cell viability, and metabolism.
•  SYTOX™ Blue Dead Cell Stain (S34857): For the 405 nm violet laser.
•  SYTOX™ AADvanced™ Dead Cell Stain (S10274, S10309): For the 488 nm laser with red emission.
•  SYTOX™ Red Dead Cell Stain (S34854): For the 633/5 nm red laser.
•  SYTOX™ Orange Dead Cell Stain (S34861): For the 532 nm green laser.
•  SYTOX™ Dead Cell Stain Sampler Kit (S34862).

Related Link
•  Learn about all SYTOX™ Products.

Hoechst 33342, Trihydrochloride, Trihydrate, 100 mg (Invitrogen™)

Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570).

Propidium Iodide ReadyProbes™ Reagent (Invitrogen™)

Propidium iodide is a commonly used dead-cell stain that emits red fluorescence when bound to DNA. Propidium iodide is also used as a counterstain in multicolor fluorescent techniques. With Propidium Iodide ReadyProbes® Reagent, we provide a room temperature-stable solution of classic DNA stain that comes in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells.

Features of Propidium Iodide ReadyProbes® Reagent:
• Ready-to-use liquid propidium iodide formulation
• Rapid staining of dead cells without wash steps
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stable at room temperature—keep handy at your work station or cell culture area

Classic Dead Cell Stain
Propidium iodide is a classic fluorescent dye used extensively for staining of dead cells (Figure 1). It exhibits a sufficiently large Stokes shift to allow simultaneous detection of nuclear DNA and FITC or Alexa Fluor® 488-labeled antibodies.

Packaged for Convenience
Propidium Iodide ReadyProbes® Reagent is supplied ready-to-use and can be stored right next to your flow cytometer or in your cell culture lab. A liquid formulation in an ultra-convenient dropper bottle (Figure 2) allows you to stain your cells whenever and wherever they are ready. Just add two drops per ml for optimally bright and distinct nuclear counter-staining.

Multiple Applications
Propidium iodide is membrane-impermeant and excluded from viable cells. It is therefore commonly used for identifying dead cells in a population. Its spectral properties, selectivity for dsDNA, and increased fluorescence when bound to DNA, make propidium iodide ideal for use with green (Alexa Fluor® 488, FITC, GFP) fluorophores in multicolor experiments. Because of its high affinity for DNA, it is also frequently used for counting cells, measuring apoptosis, and sorting cells based on DNA content.

Suggestions for Use
• In most cases, 2 drops/1×106 cells of Propidium Iodide ReadyProbes® Reagent and an incubation time of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• When bound to nucleic acids, the fluorescence excitation maximum for propidium iodide is 535 nm and the emission maximum is 617 nm. Propidium iodide can be excited with a xenon or mercury-arc lamp or with the 488 line of an argon-ion laser. Generally, propidium iodide fluorescence is detected in the FL2 channel of flow cytometers.
• As a preferred red nuclear stain in dead cell analyses using flow cytometry, Propidium Iodide ReadyProbes® Reagent is ideal for use with antibody-based applications.

See other Molecular Probes® imaging tools and reagents.