Shop All DNA⁄RNA Cell Stains

Propidium Iodide - FluoroPure™ Grade Invitrogen™

Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure grade propidium iodide is greater than or equal to 98% pure by HPLC.

Also available as a room-temperature-stable, ready-to-use solution: Propidium Iodide Ready Probes Reagent.
See other Ready Probes ready-to-use imaging reagents and accessories ›

POPO™-1 Iodide (434/456) - 1 mM Solution in DMSO Invitrogen™

POPO™-1 iodide is a high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~434/456 nm when bound to nucleic acids.

SYTO™ RNASelect™ Green Fluorescent cell Stain - 5 mM Solution in DMSO Invitrogen™

The SYTO RNASelect green fluorescent cell stain is a cell-permeant nucleic acid stain that selectively stains RNA. Although virtually nonfluorescent in the absence of nucleic acids, SYTO RNASelect stain exhibits bright green fluorescence when bound to RNA (absorption/emission maxima ~490/530 nm), but only a weak fluorescent signal when bound to DNA. Filter sets that are suitable for imaging cells labeled with fluorescein (FITC) will work well for imaging cells stained with SYTO RNASelect stain.

Vybrant™ DyeCycle™ Violet Stain Invitrogen™

Vybrant® DyeCycle™ Violet Stain is a cell permeable DNA dye that can be used for cell cycle analysis and stem cell side population by flow cytometry.

• Precise—accurate cell cycle analysis in living cells

• Safe—low cytotoxicity for cell sorting and additional live cell experiments

• Minimal compensation—easier multiplexing

• Flexible

• Simple, robust staining protocol

View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.

Precise

Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant® DyeCycle™ Violet Stain is an ideal tool for DNA content analysis in living cells since the stain is cell permeable and, after binding double-stranded DNA, emits a fluorescent signal that is proportional to the DNA mass (see figure).

Low Cytotoxicity

Unlike stains that require high concentrations or have chemical structures that are toxic to cells, Vybrant® DyeCycle™ stains exhibit relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.

Minimal Compensation

Well-suited for the popular violet laser line, the Vybrant® DyeCycle™ Violet Stain/DNA complex has fluorescence excitation and emission maxima of 369/437 nm, respectively (see figure).

The violet excitation and narrow emission of Vybrant® DyeCycle™ Violet Stain make it ideal for multiplexing due to the limited spectral overlap with other common dyes (Alexa Fluor® 488, FITC, and RPE) and fluorescent proteins (Green Fluorescent Protein (GFP) and mCherry) . Vybrant® DyeCycle™ Violet Stain can also be used with UV excitation, having emission at ~440 nm.

Flexible

Vybrant® DyeCycle™ Violet Stain has been shown to not only work for both live cells and fixed cells in cell cycle assays, but to identify stem cell side populations and early progenitors in mammalian hematopoietic tissues (see figure).

Simple, Robust Staining Protocol

For cell analysis, simply prepare flow cytometry tubes each containing 1 mL of cells suspended in complete media at a cell concentration of 1 × 106 cells/mL. To each tube, add 1 µL of Vybrant® DyeCycle™ Violet Stain and mix well. Final stain concentration is 5 µM. Incubate at 37°C for 30 minutes, protected from light. Keep cells at 37°C until acquisition. Analyze samples without washing or fixing on a flow cytometer using ~405 nm excitation and ~440 nm emission.

SYTO™ 84 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO Invitrogen™

The cell-permeant SYTO 84 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.

PO-PRO™-1 Iodide (435/455) - 1 mM Solution in DMSO Invitrogen™

One of our carbocyanine monomers, PO-PRO™-1 stain is a useful counterstain and dead cell indicator, with blue fluorescence (excitation/emission maxima ~435/455 nm).

TOTO™-3 Iodide (642/660) - 1 mM Solution in DMSO Invitrogen™

TOTO®-3 stain is a carbocyanine dimer with far-red fluorescence similar to Alexa Fluor® 647 or Cy® 5 dyes that is easily distinguished from fluorescein and rhodamine. It is useful as a nuclear counterstain and dead cell indicator, and is among the highest-sensitivity probes for nucleic acid detection.

Important Features of TOTO®-3 Include:

• Nonfluorescent in the absence of nucleic acids
• Very bright fluorescence signal
• Ideal for flow cytometry and fluorescence microscopy
• Long-wavelength fluorescence is well separated from green and red fluorophores
• Cell-impermeant
• Excitation⁄Emission: 640⁄660 nm


Many Applications
TOTO®-3 stain and the other dimeric cyanine stains have wide applicability due to their low background and bright fluorescence. Uses include staining of nucleic acids on solid supports, prestaining of samples for gel or capillary electrophoresis, and nuclear counterstaining in multiple-label experiments. Electrophoresis of samples prestained with TOTO®-3 or other dimeric cyanine dyes is possible due to the unusual stability of the dye-Nucleic Acid complexes.

TOTO®-3 Stain has High Selectivity for Nuclear Over Cytoplasmic Staining
TOTO®-3 stain gives strong and selective nuclear staining in cultured cells and in paraffin sections. TOTO®-3 stain and other carbocyanine dimers have very strong binding affinity for dsDNA, with dissociation constants in the micromolar range.

Use TOTO®-3 Stain for 2-Color Viability Staining
Simultaneous labeling with a green-fluorescent SYTO® dye and cell-impermeant TOTO®-3 or TO-PRO®-3 stains are frequently used to assess cell viability. TOTO®-3 and TO-PRO®-3 stains both have much greater extinction coefficients than that of DNA-bound propidium iodide.

Extend Your Possibilities for Multicolor Labeling
The long-wavelength fluorescence of TOTO®-3 stain is well separated from that of commonly used fluorophores, such as our popular Alexa Fluor® dyes, Oregon Green, ® fluorescein (FITC), rhodamine (TRITC), Texas Red, coumarin (AMCA), Marina Blue™ and Pacific Blue™ dyes. Additionally, long-wavelength light–absorbing dyes such as TOTO®-3 stain have the advantage that their fluorescence is usually not obscured by the autofluorescence of tissues.
Explore other probes for the nucleus

Learn more about nucleic acid detection on gels, blots, and arrays.

Read more about cyanine dyes and other nucleic acid stains.

Find out more about viability and cytotoxicity assay reagents.

Plot the TOTO®-3 spectra and check spectral compatibility with other fluorophores

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

YOYO™-3 Iodide (612/631) - 1 mM Solution in DMSO Invitrogen™

YOYO®-3 iodide is a cell-impermeant, high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~612/631 nm when bound to nucleic acids.

Hoechst 33342, Trihydrochloride, Trihydrate, 100 mg Invitrogen™

Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570).

Also available as a room-temperature-stable, ready-to-use solution: NucBlue Live Cell Ready Probes Reagent.
See other Ready Probes ready-to-use imaging reagents and accessories ›

Vybrant™ DyeCycle™ Green Stain Invitrogen™

Vybrant® DyeCycle™ Green Stain is a cell-permeable DNA dye that can be used for cell cycle analysis on a 488 nm spectral line of the argon-ion laser, which is commonly used on all flow cytometers.

• Precise—accurate cell cycle analysis in living cells

• Safe—low cytotoxicity for cell sorting and additional live cell experiments

• Simple, robust staining protocol

View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.

Precise

Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant® DyeCycle™ Green Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).

Low Cytotoxicity

Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant® DyeCycle™ Green Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.

Simple, Robust Staining Protocol

For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 106 cells/mL. To each tube add 2 µL of Vybrant® DyeCycle™ Green Stain. Final stain concentration is 10 µM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on a flow cytometer using 488 nm excitation and green emission.

SYTO™ 85 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO Invitrogen™

The cell-permeant SYTO 85 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.

SYTO™ 40 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO Invitrogen™

The cell-permeant SYTO 40 blue fluorescent nucleic acid stain exhibits bright, blue fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary from one cell type to another, we offer the SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit (S-11350) to enable researchers to find the dye that works best for a particular application.

Dihydroethidium (Hydroethidine) Invitrogen™

The superoxide indicator dihydroethdium, also called hydroethidine, exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red. Dihydtroethidium is also available in conveniently packaged 5 mM solution stabilized in DMSO (D23107).

Hoechst 33342, Trihydrochloride, Trihydrate - FluoroPure™ Grade Invitrogen™

Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure Grade Hoechst 33342 is greater than or equal to 98% pure by HPLC.

POPO™-3 Iodide (534/570) - 1 mM Solution in DMF Invitrogen™

POPO™-3 iodide is a high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~534/570 nm when bound to nucleic acids.
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