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Vivid™ Cyan Fluorescent Standard

Vivid® Fluorescent Standards represent the products formed during oxidation of Vivid® Substrates by cytochrome P450 enzymes. These standards enable quantification of enzyme activity when used as part of the Vivid® CYP450 Screening Kits. Vivid® Fluorescent Standards are included in the Vivid® Screening Kits, but may also be purchased separately. They are supplied as a dried film.

More About Vivid® CYP450 Screening Kits
Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagent. Vivid® Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

>> View all Vivid® CYP450 Screening Kits

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

Alexa Fluor™ 750 Carboxylic Acid, tris(triethylammonium) salt (Invitrogen™)

Alexa Fluor® 750 Carboxylic Acid is a non-reactive form of Alexa Fluor® 750 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 750 dye include:

• Laser line: 750 nm
• Common filter set: Cy® 7
• Excitation maxima: 749 nm
• Emission maxima: 775 nm

More About Alexa Fluor® 750 Dye
Alexa Fluor® 750 dye and its conjugates exhibit fluorescence spectra that are very similar to those of CY® 7 dye. Its fluorescence emission maximum at 775 nm is well separated from commonly used far-red fluorophores such as Alexa Fluor® 647 or allophycocyanin (APC), facilitating multicolor analysis.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Alexa Fluor™ 532 Carboxylic Acid, triethylammonium salt (Invitrogen™)

Alexa Fluor® 532 Carboxylic Acid is a non-reactive form of Alexa Fluor® 532 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 532 dye include:

• Laser line: 532 nm
• Excitation maxima: 530 nm
• Emission maxima: 554 nm

More About Alexa Fluor® 532 Dye
The Alexa Fluor® 532 dye and its conjugates have excitation and emission spectra that are intermediate between those of the green-fluorescent Alexa Fluor 488 dye and the orange-fluorescent Alexa Fluor 546 dye, making it an ideal dye for use with 532 excitation sources, including the frequency-doubled Nd-Yag laser.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Click-iT™ Plus Alexa Fluor™ 555 Picolyl Azide Toolkit (Invitrogen™)

Click-iT® Plus Alexa Fluor® picolyl azide toolkits contain everything you need to perform copper-catalyzed click reactions with compounds that are sensitive to copper, while retaining all of the benefits of the standard azide/alkyne click reaction. Picolyl azides react efficiently with chelated copper, so free copper in the click reaction can be minimized, protecting against undesired copper side reactions with proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and even small molecules (e.g., phalloidin).

• Flexible—optimize your own Click–iT® reaction for the detection or labeling of any alkyne-containing biomolecule in vitro or in cells and tissue samples
• Bright and sensitive—superior fluorescent Alexa Fluor® dyes combined with the efficiency of picolyl azide
• Convenient—contains all the buffers and reagents needed to create the optimal click reaction

Each Click-iT® Plus Picolyl Azide Toolkit includes 100 µg of Alexa Fluor® dye-picolyl azide and all of the reagents required to create a reaction cocktail with the optimal copper and chelate concentration for any click chemistry-based labeling or detection application. Guidelines for designing and optimizing the picolyl azide/alkyne click reaction of your choice are provided in the product manual. Additionally Click-iT® Plus picolyl azides offer increased sensitivity and faster reaction times compared to standard azides using identical reaction conditions.

Alexa Fluor™ 555 and Alexa Fluor™ 647 Reactive Dye Decapacks, for microarrays, includes A32756 and A32757 decapacks (Invitrogen™)

The Alexa Fluor® 555 and 647 Reactive Dye Decapack is optimized for use with aminoallyl labeling techniques. The single-use packaging of the reactive dyes eliminates the need to aliquot bulk dyes, providing convenience and ensuring maximum activity of the dyes. The specially packaged Alexa Fluor® dyes are compatible with both aminoallyl dUTP- and aminoallyl UTP-modified nucleotides as well as most commercially available aminoallyl-based labeling kits.

Alexa Fluor™ 568 Carboxylic Acid, tris(triethylammonium) salt (Invitrogen™)

Alexa Fluor® 568 Carboxylic Acid is a non-reactive form of Alexa Fluor® 568 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 568 dye include:

• Laser line: 568 nm
• Common filter set: Texas Red
• Excitation maxima: 578 nm
• Emission maxima: 603 nm

More About Alexa Fluor® 568 Dye
Alexa Fluor® 568 dye is a bright, orange- or orange-red-fluorescent dye with excitation ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser and matched well with RFP filter sets. Alexa Fluor® 568 dye is commonly used with DAPI, and Alexa Fluor® 488 and 647 dyes for multiplexing. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Fluorescein - NIST-Traceable Standard - Nominal concentration 50µM - Special Packaging (Invitrogen™)

The NIST-traceable fluorescein standard is supplied as a calibrated 50 µM solution of fluorescein in 100 mM sodium borate buffer, pH 9.5. Under these conditions, fluorescein is completely ionized and is therefore in its most fluorescent form. This standard meets the stringent criteria established by the National Institute of Standards and Technology (NIST) and is directly traceable to Standard Reference Material 1932 (SRM 1932). The NIST-traceable fluorescent standard can be used to assess day-to-day or experiment-to-experiment variation in fluorescence-based instrumentation as well as to determine the Molecules of Equivalent Soluble Fluorophore (MESF) value for an experimental solution.

Reference Dye Sampler Kit - Five 1 mM Solution, 1 mL each (Invitrogen™)

The Reference Dye Sampler Kit provides samples of five extensively characterized fluorescence standards (quinine sulfate, fluorescein, carboxy-tetramethylrhodamine, sulforhodamine 101, nile blue) packaged as 1 mM solutions with emission spectra covering the entire visible wavelength range.

Vivid™ Green Fluorescent Standard

Vivid® Fluorescent Standards represent the products formed during oxidation of Vivid® Substrates by cytochrome P450 enzymes. These standards enable quantification of enzyme activity when used as part of the Vivid® CYP450 Screening Kits. Vivid® Fluorescent Standards are included in the Vivid® Screening Kits, but may also be purchased separately. They are supplied as a dried film.

More About Vivid® CYP450 Screening Kits
Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagent. Vivid® Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

>> View all Vivid® CYP450 Screening Kits

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

Alexa Fluor™ 350 Carboxylic Acid, bis(triethylammonium) salt (Invitrogen™)

Alexa Fluor® 350 Carboxylic Acid is a non-reactive form of Alexa Fluor® 350 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 350 dye include:

• Laser line: 350 nm
• Common filter set: DAPI
• Excitation maxima: 346 nm
• Emission maxima: 442 nm

More About Alexa Fluor® 350 Dye
Alexa Fluor® 350 dye is a blue-fluorescent dye with moderate photostability and excitation that matches the 350 nm laser line. As the brightest dye available in the blue channel, it is best used for high-abundance targets like actin or tubulin. Alexa Fluor® 350 dye is commonly used with Alexa Fluor® 488, 594, and 647 dyes for multiplexing. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Alexa Fluor™ 546 Carboxylic Acid, tris(triethylammonium) salt (Invitrogen™)

Alexa Fluor® 546 Carboxylic Acid is a non-reactive form of Alexa Fluor® 546 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 546 dye include:

• Laser line: 546 nm
• Common filter set: TRITC (tetramethylrhodamine)
• Excitation maxima: 556 nm
• Emission maxima: 573 nm

More About Alexa Fluor® 546 Dye
Alexa Fluor® 546 dye is a bright, yellow-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. Alexa Fuor® 546 serves as an alternative for tetramethylrhodamine or Cy®3 dye, with higher initial intensity than Alexa Fluor® 555 dye, but lower photostability in buffer or antifade. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Qdot™ 545 ITK™ Organic Quantum Dots (Invitrogen™)

Qdot® 545 ITK™ organic quantum dots are the ideal starting material for applications calling for organic solvents such as hexane, toluene, dichloromethane, or chloroform. These materials are provided as solutions in decane, and are not water soluble. These Qdot® ITK™ organic quantum dots are designed for researchers who want to use their own methods for transferring quantum dot materials into water. Our Qdot® ITK™ organic quantum dots are provided as 1 µM solutions and are available in 7 colors of Qdot® probes.

Important Features of Qdot® ITK™ Organic Quantum Dots:
• Qdot® 545 ITK™ organic quantum dot has emission maxima of ~545 nm
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large stokes shift
• Available in multiple colors
• Ideal for various labeling and tracking applications


Properties of Qdot® Nanocrystals
Qdot® probes are ideal for imaging and labeling applications that require bright fluorescent signals and/or real-time tracking. Unique among fluorescent reagents, all nine available colors of Qdot® probes can be simultaneously excited with a single (UV to blue-green) light source. This property makes these reagents excellent for economical and user-friendly multiplexing applications. Qdot® labels are based on semiconductor nanotechnology and are similar in scale to moderately sized proteins.

About the Innovator’s Tool Kit Qdot® ITK™ Reagents
These Qdot®ITK™ probes are ideal for researchers who wish to prepare specific (non-stocked) conjugates for their applications and need customizable conjugation functionality.

Other Forms of Qdot® Nanocrystals are Available
In addition to the organic form, we offer Qdot® ITK™ quantum dots with carboxyl and amine-derivatized PEG modifications. We’ve also developed a wide range of Qdot® nanocrystals conjugates and labeling kits. Investigate the properties of Qdot® nanocrystals or read the Molecular Probes® Handbook Section 6.6—Qdot® Nanocrystals to find out more.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Click-iT™ Plus Alexa Fluor™ 647 Picolyl Azide Toolkit (Invitrogen™)

Click-iT® Plus Alexa Fluor® picolyl azide toolkits contain everything you need to perform copper-catalyzed click reactions with compounds that are sensitive to copper, while retaining all of the benefits of the standard azide/alkyne click reaction. Picolyl azides react efficiently with chelated copper, so free copper in the click reaction can be minimized, protecting against undesired copper side reactions with proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and even small molecules (e.g., phalloidin).

• Flexible—optimize your own Click–iT® reaction for the detection or labeling of any alkyne-containing biomolecule in vitro or in cells and tissue samples
• Bright and sensitive—superior fluorescent Alexa Flour® dyes combined with the efficiency of picolyl azide
• Convenient—contains all the buffers and reagents needed to create the optimal click reaction

Each Click-iT® Plus Picolyl Azide Toolkit includes 100 µg of Alexa Fluor® dye-picolyl azide and all of the reagents required to create a reaction cocktail with the optimal copper and chelate concentration for any click chemistry-based labeling or detection application. Guidelines for designing and optimizing the picolyl azide/alkyne click reaction of your choice are provided in the product manual. Additionally Click-iT® Plus picolyl azides offer increased sensitivity and faster reaction times compared to standard azides using identical reaction conditions.

Alexa Fluor™ 555 Decapack Set (Invitrogen™)

The Alexa Fluor® 555 Reactive Dye Decapack is optimized for use with aminoallyl labeling techniques. The single-use packaging of the reactive dye eliminates the need to aliquot bulk dyes, providing convenience and ensuring maximum activity of the dyes. The specially packaged Alexa Fluor® 555 dye is compatible with both aminoallyl dUTP- and aminoallyl UTP-modified nucleotides as well as most commercially available aminoallyl-based labeling kits.

Alexa Fluor™ 594 Carboxylic Acid, tris(triethylammonium) salt (Invitrogen™)

Alexa Fluor® 594 Carboxylic Acid is a non-reactive form of Alexa Fluor® 594 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 594 dye include:

• Laser line: 561 or 594 nm
• Common filter set: Texas Red
• Excitation maxima: 590 nm
• Emission maxima: 617 nm

More About Alexa Fluor® 594 Dye
Alexa Fluor® 594 dye is a bright, red-fluorescent dye that can be excited using the 561 or 594 nm laser lines. It serves as a direct replacement for Texas Red® dye with bright signal and excellent photostability, especially in fixed cells. Alexa Fluor® 594 dye is commonly used with DAPI, and Alexa Fluor® 488 and 647 dyes for multiplexing. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.