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Alexa Fluor™ 488 Carboxylic Acid, tris(triethylammonium) salt Invitrogen™

Alexa Fluor® 488 Carboxylic Acid is a non-reactive form of Alexa Fluor® 488 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 488 dye include:

• Laser line: 488 nm
• Common filter set: FITC (fluorescein)
• Excitation maxima: 500 nm
• Emission maxima: 525 nm

More About Alexa Fluor® 488 Dye
Alexa Fluor® 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. It is the brightest and most photostable green dye for sensitive detection in live or fixed cells. Alexa Fluor® 488 dye is commonly used with DAPI, and Alexa Fluor® 594 and 647 dyes for multiplexing. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Alexa Fluor™ 532 Carboxylic Acid, triethylammonium salt Invitrogen™

Alexa Fluor® 532 Carboxylic Acid is a non-reactive form of Alexa Fluor® 532 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 532 dye include:

• Laser line: 532 nm
• Excitation maxima: 530 nm
• Emission maxima: 554 nm

More About Alexa Fluor® 532 Dye
The Alexa Fluor® 532 dye and its conjugates have excitation and emission spectra that are intermediate between those of the green-fluorescent Alexa Fluor 488 dye and the orange-fluorescent Alexa Fluor 546 dye, making it an ideal dye for use with 532 excitation sources, including the frequency-doubled Nd-Yag laser.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Alexa Fluor™ 546 Carboxylic Acid, tris(triethylammonium) salt Invitrogen™

Alexa Fluor® 546 Carboxylic Acid is a non-reactive form of Alexa Fluor® 546 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 546 dye include:

• Laser line: 546 nm
• Common filter set: TRITC (tetramethylrhodamine)
• Excitation maxima: 556 nm
• Emission maxima: 573 nm

More About Alexa Fluor® 546 Dye
Alexa Fluor® 546 dye is a bright, yellow-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. Alexa Fuor® 546 serves as an alternative for tetramethylrhodamine or Cy®3 dye, with higher initial intensity than Alexa Fluor® 555 dye, but lower photostability in buffer or antifade. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Alexa Fluor™ 555 Carboxylic Acid, tris(triethylammonium) salt Invitrogen™

Alexa Fluor® 555 Carboxylic Acid is a non-reactive form of Alexa Fluor® 555 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 555 dye include:

• Laser line: 488 or 532 nm
• Common filter set: TRITC (tetramethylrhodamine)
• Excitation maxima: 555 nm
• Emission maxima: 580 nm

More About Alexa Fluor® 555 Dye
Alexa Fluor® 555 dye is a bright, orange-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. It is a recommended replacement for Cy®3 dye or tetramethylrhodamine, with greater photostability than Cy®3 dye in live and fixed cells. Alexa Fluor® 555 dye is commonly used with DAPI, and Alexa Fluor® 488 and 647 dyes for multiplexing. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Alexa Fluor™ 568 Carboxylic Acid, tris(triethylammonium) salt Invitrogen™

Alexa Fluor® 568 Carboxylic Acid is a non-reactive form of Alexa Fluor® 568 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 568 dye include:

• Laser line: 568 nm
• Common filter set: Texas Red
• Excitation maxima: 578 nm
• Emission maxima: 603 nm

More About Alexa Fluor® 568 Dye
Alexa Fluor® 568 dye is a bright, orange- or orange-red-fluorescent dye with excitation ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser and matched well with RFP filter sets. Alexa Fluor® 568 dye is commonly used with DAPI, and Alexa Fluor® 488 and 647 dyes for multiplexing. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Alexa Fluor™ 594 Carboxylic Acid, tris(triethylammonium) salt Invitrogen™

Alexa Fluor® 594 Carboxylic Acid is a non-reactive form of Alexa Fluor® 594 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 594 dye include:

• Laser line: 561 or 594 nm
• Common filter set: Texas Red
• Excitation maxima: 590 nm
• Emission maxima: 617 nm

More About Alexa Fluor® 594 Dye
Alexa Fluor® 594 dye is a bright, red-fluorescent dye that can be excited using the 561 or 594 nm laser lines. It serves as a direct replacement for Texas Red® dye with bright signal and excellent photostability, especially in fixed cells. Alexa Fluor® 594 dye is commonly used with DAPI, and Alexa Fluor® 488 and 647 dyes for multiplexing. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Alexa Fluor™ 633 Carboxylic Acid, tris(triethylammonium) salt Invitrogen™

Alexa Fluor® 633 Carboxylic Acid is a non-reactive form of Alexa Fluor® 633 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 633 dye include:

• Laser line: 633 or 635 nm
• Excitation maxima: 632 nm
• Emission maxima: 647 nm

More About Alexa Fluor® 633 Dye
The Alexa Fluor 633 dye and its conjugates are a perfect match to the 633 nm spectra line of the He-Ne laser or the 635 nm spectral line of red diode lasers. This and other far-red fluorescent Alexa Fluor® dyes are important labels for fluorescence imaging because their spectra are beyond the range of most sample autofluorescence.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Fluorescein - Reference Standard Invitrogen™

Fluorescein can be used as a reference standard for ether, ester and phosphate substrates derived from this green-fluorescent dye.

See our full collection of microscope calibration reagents ›

Vivid™ Green Fluorescent Standard

Vivid® Fluorescent Standards represent the products formed during oxidation of Vivid® Substrates by cytochrome P450 enzymes. These standards enable quantification of enzyme activity when used as part of the Vivid® CYP450 Screening Kits. Vivid® Fluorescent Standards are included in the Vivid® Screening Kits, but may also be purchased separately. They are supplied as a dried film.

More About Vivid® CYP450 Screening Kits
Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagent. Vivid® Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

>> View all Vivid® CYP450 Screening Kits

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

Alexa Fluor™ 647 Carboxylic Acid, tris(triethylammonium) salt Invitrogen™

Alexa Fluor® 647 Carboxylic Acid is a non-reactive form of Alexa Fluor® 647 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 647 dye include:

• Laser line: 647 nm
• Common filter set: Cy® 5
• Excitation maxima: 650 nm
• Emission maxima: 665 nm

More About Alexa Fluor® 647 Dye
Alexa Fluor® 647 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. It is ideal for imaging low-abundance targets, especially in fixed cells. Alexa Fluor® 647 dye is commonly used with DAPI, and Alexa Fluor® 488 and 594 dyes for multiplexing. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Fluorescein - NIST-Traceable Standard - Nominal concentration 50µM - Special Packaging Invitrogen™

The NIST-traceable fluorescein standard is supplied as a calibrated 50 µM solution of fluorescein in 100 mM sodium borate buffer, pH 9.5. Under these conditions, fluorescein is completely ionized and is therefore in its most fluorescent form. This standard meets the stringent criteria established by the National Institute of Standards and Technology (NIST) and is directly traceable to Standard Reference Material 1932 (SRM 1932). The NIST-traceable fluorescent standard can be used to assess day-to-day or experiment-to-experiment variation in fluorescence-based instrumentation as well as to determine the Molecules of Equivalent Soluble Fluorophore (MESF) value for an experimental solution.

See our full collection of microscope calibration reagents ›

Click-iT™ Plus Alexa Fluor™ 555 Picolyl Azide Toolkit Invitrogen™

Click-iT® Plus Alexa Fluor® picolyl azide toolkits contain everything you need to perform copper-catalyzed click reactions with compounds that are sensitive to copper, while retaining all of the benefits of the standard azide/alkyne click reaction. Picolyl azides react efficiently with chelated copper, so free copper in the click reaction can be minimized, protecting against undesired copper side reactions with proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and even small molecules (e.g., phalloidin).

• Flexible—optimize your own Click–iT® reaction for the detection or labeling of any alkyne-containing biomolecule in vitro or in cells and tissue samples
• Bright and sensitive—superior fluorescent Alexa Fluor® dyes combined with the efficiency of picolyl azide
• Convenient—contains all the buffers and reagents needed to create the optimal click reaction

Each Click-iT® Plus Picolyl Azide Toolkit includes 100 µg of Alexa Fluor® dye-picolyl azide and all of the reagents required to create a reaction cocktail with the optimal copper and chelate concentration for any click chemistry-based labeling or detection application. Guidelines for designing and optimizing the picolyl azide/alkyne click reaction of your choice are provided in the product manual. Additionally Click-iT® Plus picolyl azides offer increased sensitivity and faster reaction times compared to standard azides using identical reaction conditions.

Vivid™ Blue Fluorescent Standard

Vivid® Fluorescent Standards represent the products formed during oxidation of Vivid® Substrates by cytochrome P450 enzymes. These standards enable quantification of enzyme activity when used as part of the Vivid® CYP450 Screening Kits. Vivid® Fluorescent Standards are included in the Vivid® Screening Kits, but may also be purchased separately. They are supplied as a dried film.

More About Vivid® CYP450 Screening Kits
Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagent. Vivid® Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

>> View all Vivid® CYP450 Screening Kits

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

Alexa Fluor™ 750 Carboxylic Acid, tris(triethylammonium) salt Invitrogen™

Alexa Fluor® 750 Carboxylic Acid is a non-reactive form of Alexa Fluor® 750 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 750 dye include:

• Laser line: 750 nm
• Common filter set: Cy® 7
• Excitation maxima: 749 nm
• Emission maxima: 775 nm

More About Alexa Fluor® 750 Dye
Alexa Fluor® 750 dye and its conjugates exhibit fluorescence spectra that are very similar to those of CY® 7 dye. Its fluorescence emission maximum at 775 nm is well separated from commonly used far-red fluorophores such as Alexa Fluor® 647 or allophycocyanin (APC), facilitating multicolor analysis.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

Click-iT™ Plus Alexa Fluor™ 647 Picolyl Azide Toolkit Invitrogen™

Click-iT® Plus Alexa Fluor® picolyl azide toolkits contain everything you need to perform copper-catalyzed click reactions with compounds that are sensitive to copper, while retaining all of the benefits of the standard azide/alkyne click reaction. Picolyl azides react efficiently with chelated copper, so free copper in the click reaction can be minimized, protecting against undesired copper side reactions with proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and even small molecules (e.g., phalloidin).

• Flexible—optimize your own Click–iT® reaction for the detection or labeling of any alkyne-containing biomolecule in vitro or in cells and tissue samples
• Bright and sensitive—superior fluorescent Alexa Flour® dyes combined with the efficiency of picolyl azide
• Convenient—contains all the buffers and reagents needed to create the optimal click reaction

Each Click-iT® Plus Picolyl Azide Toolkit includes 100 µg of Alexa Fluor® dye-picolyl azide and all of the reagents required to create a reaction cocktail with the optimal copper and chelate concentration for any click chemistry-based labeling or detection application. Guidelines for designing and optimizing the picolyl azide/alkyne click reaction of your choice are provided in the product manual. Additionally Click-iT® Plus picolyl azides offer increased sensitivity and faster reaction times compared to standard azides using identical reaction conditions.
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