Shop All Biotin Labeling Kits & Reagents

D-Biotin (Invitrogen™)

D-biotin binds with high affinity to streptavidin, avidin and NeutrAvidin® biotin-binding proteins.

DMNP-EDTA (1-(4,5-Dimethoxy-2-Nitrophenyl)-1,2-Diaminoethane-N,N,N',N'-Tetraacetic Acid), cell impermeant (Invitrogen™)

The caged Ca2+ chelator, DMNP-EDTA (also known as DM-Nitrophen®) upon photolysis, the Kd for Ca2+ increases from 5 nM to 3 mM. Thus, photolysis of DMNP-EDTA complexed with Ca2+ results in a pulse of free Ca2+.

Biotin Azide (PEG4 carboxamide-6-Azidohexanyl Biotin) (Invitrogen™)

The hapten, biotin azide is reactive with terminal alkynes via a copper-catalyzed click reaction. Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin® biotin-binding protein.

Qdot™ 605 Biotin Conjugate Kit (Invitrogen™)

The biotin-labeled Qdot® 605 nanocrystals are available for detecting streptavidin probes or for creating noncovalent conjugates with streptavidin-labeled molecules or with other biotinylated molecules using a streptavidin bridge. The product is provided as 250 µL of a 2 µM solution and includes 30 mL of Qdot® incubation buffer.

Fluorescein biotin, 5-((N-(5-(N-(6-(biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl)fluorescein (Invitrogen™)

Fluorescein biotin was developed as an alternative to radioactive biotin for detecting and quantitating biotin-binding sites by either fluorescence or absorbance. Upon binding to avidin or streptavidin, the fluorescence or absorbance of this molecule is quenched.

Biotin-HRP (biotinylated peroxidase) (Invitrogen™)

Reactivity: Reacts with streptavidin and avidin conjugates.

Pierce™ Biotin-Fluorescein Conjugate (Thermo Scientific™)

Thermo Scientific Pierce Biotinylated Proteins include biotin-labeled proteins (BSA), enzymes (HRP, AP) and fluorophores (FITC) for use as controls or signal amplification in IHC via avidin-biotin complex (ABC) techniques.

Biotinylated Fluorescein (FITC) cannot be used to polymerize and amplify the signal to the same degree as biotinylated enzymes because each fluorescent molecule is tagged with only one biotin molecule. However, the fluorescent variant of traditional ABC staining is possible with this unique molecule.

Biotinylated FITC—amplify the fluorescein signal of biotinylated primary antibodies by using avidin or streptavidin plus biotinylated fluorescein (B-FITC)

Biotin, also known as vitamin H, is a small molecule (MW 244.3) that is present in tiny amounts in all living cells and is critical for a number of biological processes. The valeric acid side chain of the biotin molecule can be derivatized in order to incorporate various reactive groups that are used to attach biotin to other molecules. In the context of immunohistochemistry (IHC), biotin is conjugated to antibodies or to the enzyme reporters used to detect target antigens.

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Biotin Alkyne (PEG4 carboxamide-Propargyl Biotin) (Invitrogen™)

The hapten, biotin azide is reactive with terminal via a copper-catalyzed click reaction. Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin® biotin-binding protein.

Qdot™ 655 Biotin Conjugate Kit (Invitrogen™)

The biotin-labeled Qdot® 655 nanocrystals are available for detecting streptavidin probes or for creating noncovalent conjugates with streptavidin-labeled molecules or with other biotinylated molecules using a streptavidin bridge. The product is provided as 250 µL of a 2 µM solution and includes 30 mL of Qdot® incubation buffer.

Click-IT™ Biotin Protein Analysis Detection Kit (Invitrogen™)

The Click-iT® Biotin Glycoprotein Detection Kit provides the second part of a simple and robust technique to identify and characterize glycoproteins by Western blot. In step two, after the incorporation of an azide moiety into protein glycan structures with either a Click-iT® metabolic labeling reagent or the Click-iT® Enzymatic Labeling system, the azide-modified glycoproteins are detected via the chemoselective ligation or click reaction between an azide and an alkyne. With this technique, detection sensitivity is in the low femptomole range and biotin-labeled samples can be detected before or after probing the Western with a primary antibody.

Biotin-4-Fluorescein (Invitrogen™)

Biotin-4-fluorescein can be used to quantitate available biotin-binding sites or the concentration of avidin or streptavidin through the strong quenching associated when this molecule binds to avidin.