Shop All Pre-Cast Protein Gels

NuPAGE™ Bis-Tris Welcome Pack, 10%, 12-well (Invitrogen™)

The NuPAGE Bis-Tris Welcome Pack provides all of the necessary NuPAGE gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The NuPAGE Bis-Tris Welcome Pack contains:
Mini Gel Tank
• NuPAGE Bis-Tris mini gels (2 boxes, 20 gels)
NuPAGE MES SDS Running Buffer (20X)
NuPAGE LDS Sample Buffer (4X)
NuPAGE Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.

About NuPAGE Bis-Tris gels
Invitrogen NuPAGE Bis-Tris Protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use.

Transfer
For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ 6% Tris-Glycine Mini Gels, WedgeWell™ format, 10-well (Invitrogen™)

Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity. Novex Tris-Glycine Mini Gels are based on traditional Laemmli protein electrophoresis that allow the use of Laemmli sample and running buffers.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine gels, WedgeWell format, include:
Improved shelf life—store gels for up to 6 months at 4°C
Diversity—use for native and denaturing protein assays
Wedge-shaped wells—easily load up to two times more sample volume
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.

Run your proteins in native or denatured form
Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

Convenient to use
Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer when preforming traditional wet transfer using the Invitrogen Mini Blot Module. Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ Bis-Tris Welcome Pack, 10%, 15-well (Invitrogen™)

The NuPAGE Bis-Tris Welcome Pack provides all of the necessary NuPAGE gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The NuPAGE Bis-Tris Welcome Pack contains:
Mini Gel Tank
• NuPAGE Bis-Tris mini gels (2 boxes, 20 gels)
NuPAGE MES SDS Running Buffer (20X)
NuPAGE LDS Sample Buffer (4X)
NuPAGE Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.

About NuPAGE Bis-Tris gels
Invitrogen NuPAGE Bis-Tris Protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use.

Transfer
For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ 6% Tris-Glycine Mini Gels, WedgeWell™ format, 12-well (Invitrogen™)

Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity. Novex Tris-Glycine Mini Gels are based on traditional Laemmli protein electrophoresis that allow the use of Laemmli sample and running buffers.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine gels, WedgeWell format, include:
Improved shelf life—store gels for up to 6 months at 4°C
Diversity—use for native and denaturing protein assays
Wedge-shaped wells—easily load up to two times more sample volume
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.

Run your proteins in native or denatured form
Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

Convenient to use
Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer when preforming traditional wet transfer using the Invitrogen Mini Blot Module. Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ 10% Bis-Tris Protein Gels, 1.5 mm, 10-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module or XCell II Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

Bolt™ Welcome Pack, 8%, 10-well (Invitrogen™)

The Bolt Welcome Pack provides all of the necessary Bolt gels and buffers you will need to begin using the Mini Gel Tank. The Welcome Pack contains:

Mini Gel Tank
• Bolt Bis-Tris Plus gels (2 boxes, 20 gels)
Bolt MES Running Buffer (20X)
Bolt LDS Sample Buffer (4X)
Bolt Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies used.

About Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8, 10, 12, and 4-12% gradient and multiple well formats. Bolt Bis-Tris gels feature wedge-shaped wells, which can easily load up to two times more sample volume allowing for detection of proteins in very dilute samples or visualization of low-abundance proteins.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ 6% Tris-Glycine Mini Gels, WedgeWell™ format, 15-well (Invitrogen™)

Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity. Novex Tris-Glycine Mini Gels are based on traditional Laemmli protein electrophoresis that allow the use of Laemmli sample and running buffers.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine gels, WedgeWell format, include:
Improved shelf life—store gels for up to 6 months at 4°C
Diversity—use for native and denaturing protein assays
Wedge-shaped wells—easily load up to two times more sample volume
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.

Run your proteins in native or denatured form
Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

Convenient to use
Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer when preforming traditional wet transfer using the Invitrogen Mini Blot Module. Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ Tricine Welcome Pack, 16%, 15-well (Invitrogen™)

The Novex Tricine Welcome Pack provides all of the necessary Novex Tricine gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The Novex Tricine Welcome Pack contains:
Mini Gel Tank
• Novex Tricine Mini Gels (2 boxes, 20 gels)
Novex Tricine SDS Running Buffer (10X)
Novex Tricine SDS Sample Buffer (2X)
NuPAGE Sample Reducing Agent (10X)
Spectra Multicolor Low Range Protein Ladder

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.

About Novex Tricine gels
Novex Tricine gels provide separation of low molecular weight proteins and peptides. The Tricine system is a modification of the tris-glycine discontinuous buffer system developed by Schaegger and von Jagow specifically for peptides and low molecular weight proteins. In this system the tricine replaces the glycine in the running buffer, resulting in more efficient stacking and de-stacking of low molecular weight proteins and higher resolution of smaller peptides.

Transfer
For transfer of proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be performed using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0 mm, 12-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use.

Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Learn more about all of our NuPAGE Bis-Tris gels >
View migration charts ›

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

We also provide NuPAGE Tris-Acetate gels for separating larger proteins. For classic Laemmli-based Tris-glycine electrophoresis, we provide Novex Tris-Glycine gels.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the XCell II Blot Module or the Mini Blot Module.

Related links
Overview of 1D Protein Electrophoresis
Comparison of NuPAGE Tris-Bis vs. traditional Tris-glycine gels

NuPAGE™ 10% Bis-Tris Protein Gels, 1.0 mm, 12-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module or XCell II Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ 10% Bis-Tris Protein Gels, 1.5 mm, 15-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module or XCell II Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ 12% Bis-Tris Protein Gels, 1.0 mm, 17-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module or XCell II Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.5 mm, 10-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use.

Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Learn more about all of our NuPAGE Bis-Tris gels >
View migration charts ›

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

We also provide NuPAGE Tris-Acetate gels for separating larger proteins. For classic Laemmli-based Tris-glycine electrophoresis, we provide Novex Tris-Glycine gels.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the XCell II Blot Module or the Mini Blot Module.

Related links
Overview of 1D Protein Electrophoresis
Comparison of NuPAGE Tris-Bis vs. traditional Tris-glycine gels

Bolt™ Welcome Pack, 8%, 12-well (Invitrogen™)

The Bolt Welcome Pack provides all of the necessary Bolt gels and buffers you will need to begin using the Mini Gel Tank. The Welcome Pack contains:

Mini Gel Tank
• Bolt Bis-Tris Plus gels (2 boxes, 20 gels)
Bolt MES Running Buffer (20X)
Bolt LDS Sample Buffer (4X)
Bolt Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies used.

About Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8, 10, 12, and 4-12% gradient and multiple well formats. Bolt Bis-Tris gels feature wedge-shaped wells, which can easily load up to two times more sample volume allowing for detection of proteins in very dilute samples or visualization of low-abundance proteins.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ WedgeWell™ Welcome Pack, 6%, 10-well (Invitrogen™)

The Novex Tris-Glycine Welcome Pack provides all of the necessary Novex Tris-glycine gels, buffers, and reagents you need to begin using the Mini Gel Tank.

The Novex Tris-Glycine Welcome Pack contains:
Mini Gel Tank
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
Novex Tris-Glycine SDS Running Buffer (10X)
Novex Tris-Glycine SDS Sample Buffer (2X)
NuPAGE Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.

About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer or Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.