Shop All Pre-Cast Protein Gels

NuPAGE™ Bis-Tris Welcome Pack, 10%, 12-well (Invitrogen™)

The NuPAGE Bis-Tris Welcome Pack provides all of the necessary NuPAGE gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The NuPAGE Bis-Tris Welcome Pack contains:
Mini Gel Tank
• NuPAGE Bis-Tris mini gels (2 boxes, 20 gels)
NuPAGE MES SDS Running Buffer (20X)
NuPAGE LDS Sample Buffer (4X)
NuPAGE Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.

About NuPAGE Bis-Tris gels
Invitrogen NuPAGE Bis-Tris Protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use.

Transfer
For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ 4-20% Tris-Glycine Mini Protein Gels, 1.0 mm, 1-well (Invitrogen™)

Novex® 4–20% Tris-Glycine Mini Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis. Novex® Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands. Each box contains 10 gels. Features of these gels:

•   Individually packaged gels for optimal experiment design
•   Compatible with most protein standards
•   Usable for native and denaturing protein assays

Choose the right gel format for your experiments
Novex® Tris-Glycine gels come in a variety of fixed concentrations from 4% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can also select between our mini (8 cm x 8 cm) and our midi (8 cm x 13 cm) sizes, as well as multiple well-formats from 1 well to 26 wells.

For best results, most Novex® Tris-Glycine gels should be stored at 4°C and used within 8 weeks of purchase. Novex® 16% and 18% Tris-Glycine gels should be used within 4 weeks of purchase.

Run your proteins in native or denatured form

Novex® Tris-Glycine gels do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex® Tris-Glycine SDS Sample Buffer and Novex® Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex® Tris-Glycine Native Sample Buffer and Novex® Tris-Glycine Native Running Buffer.

For transferring your proteins to membrane, use Novex® Tris-Glycine Transfer Buffer. Transfers can be done using the Xcell II™ Blot Module or using the iBlot® Gel Transfer Device.

Related Links

•   Use our search tool to find the Novex® Tris-Glycine gel that is right for you.
•   See protein migration ranges on Novex® Tris-Glycine gels.

NuPAGE™ Large Protein Sensitive Staining Kit (Invitrogen™)

The HiMark™ Pre-Stained and Unstained High Molecular Weight Protein Standards are specifically designed for large protein analysis on NuPAGE® Novex Tris-Acetate Gels under denaturing conditions. Both standards offer a time-saving load-and-go format.

The HiMark™ Pre-stained Standard includes one pink colored orientation band and eight blue bands ranging in apparent molecular weight of 31 - 460 kDa* in NuPAGE® Tris-Acetate Gel/SDS buffer system (Figure 1). It's suitable for monitoring electrophoresis, western transfer, and approximating molecular weight of unknown big proteins.

The HiMark Unstained Standard, along with the NuPAGE® Tris-Acetate Gel/SDS buffer system, offers the highest accuracy for molecular weight estimation of large proteins. It consists of nine proteins, ranging in size from 40 to 500 kDa in NuPAGE® Novex 3-8% and 7% Tris-Acetate Gel/SDS buffer systems (Figure 2). Visualize with Coomassie® stain, silver, and fluorescent protein stains post-electrophoresis. A downloadable molecular weight calculator is designed for easy and accurate MW estimation of large proteins using the HiMark™ Unstained Standard on a NuPAGE® Tris-Acetate Gel.**

Several NuPAGE® Large Protein Starter Kits are available to provide you with all necessary reagents and optimized protocols for highresolution large protein separation and analysis (Table 1). Each kit includes two boxes of NuPAGE® 3-8% Tris-Acetate Gels, Tris-Acetate SDS Buffer Kit, a staining reagent or blotting membranes, and a vial of the appropriate HiMark™ Standard.

NuPAGE™ 10% Bis-Tris Midi Protein Gels, 12+2-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

NuPAGE Bis-Tris Midi gels can be used with the XCell SureLock Midi Cell gel apparatuses or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the Bio-Rad Criterion Cell using NuPAGE Transfer buffer.

Related links
Overview of 1D Protein Electrophoresis

Novex™ 12% Tris-Glycine Plus Midi Gels, 20-well, w/adapters (Invitrogen™)

Novex Tris-Glycine Plus Midi Protein gels are improved polyacrylamide gels based on traditional Laemmli protein electrophoresis technology, allowing the use of Laemmli sample and running buffers. The gels offer reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Plus Midi gels are a 1.0-mm thick, wider format (8 cm x 13 cm) gel for higher throughput electrophoresis of protein samples.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine Plus gels include:
Improved shelf life—store gels for up to 12 months at 4°C
Flexibility—use for native and denaturing protein assays
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine Plus Midi gels come in fixed concentrations of 10% and 12%, as well as gradient concentrations of 4–12%, 4–20%, and 8–16%. Select from our many well formats, including 12+2-, 20-, and 26-well.

Run your proteins in native or denatured form
Novex Tris-Glycine Plus gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer. The gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer when performing traditional wet transfer. Rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ 10-20% Tris-Glycine Mini Gels, WedgeWell™ format, 15-well (Invitrogen™)

Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity. Novex Tris-Glycine Mini Gels are based on traditional Laemmli protein electrophoresis that allow the use of Laemmli sample and running buffers.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine gels, WedgeWell format, include:
Improved shelf life—store gels for up to 6 months at 4°C
Diversity—use for native and denaturing protein assays
Wedge-shaped wells—easily load up to two times more sample volume
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.

Run your proteins in native or denatured form
Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

Convenient to use
Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer when preforming traditional wet transfer using the Invitrogen Mini Blot Module. Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0 mm, 9-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module or XCell II Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

Novex™ 4-12% Tris-Glycine Plus Midi Gels, 12+2-well, w/adapters (Invitrogen™)

Novex Tris-Glycine Plus Midi Protein gels are improved polyacrylamide gels based on traditional Laemmli protein electrophoresis technology, allowing the use of Laemmli sample and running buffers. The gels offer reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Plus Midi gels are a 1.0-mm thick, wider format (8 cm x 13 cm) gel for higher throughput electrophoresis of protein samples.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine Plus gels include:
Improved shelf life—store gels for up to 12 months at 4°C
Flexibility—use for native and denaturing protein assays
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine Plus Midi gels come in fixed concentrations of 10% and 12%, as well as gradient concentrations of 4–12%, 4–20%, and 8–16%. Select from our many well formats, including 12+2-, 20-, and 26-well.

Run your proteins in native or denatured form
Novex Tris-Glycine Plus gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer. The gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer when performing traditional wet transfer. Rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ 8-16% Tris-Glycine Plus Midi Gels, 12+2-well (Invitrogen™)

Novex Tris-Glycine Plus Midi Protein gels are improved polyacrylamide gels based on traditional Laemmli protein electrophoresis technology, allowing the use of Laemmli sample and running buffers. The gels offer reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Plus Midi gels are a 1.0-mm thick, wider format (8 cm x 13 cm) gel for higher throughput electrophoresis of protein samples.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine Plus gels include:
Improved shelf life—store gels for up to 12 months at 4°C
Flexibility—use for native and denaturing protein assays
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine Plus Midi gels come in fixed concentrations of 10% and 12%, as well as gradient concentrations of 4–12%, 4–20%, and 8–16%. Select from our many well formats, including 12+2-, 20-, and 26-well.

Run your proteins in native or denatured form
Novex Tris-Glycine Plus gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer. The gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer when performing traditional wet transfer. Rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Bolt™ 12% Bis-Tris Plus Gels, 15-well (Invitrogen™)

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Similar to NuPAGE Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications with increased loading capacity. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Use Bolt Bis-Tris Plus gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four gel types and multiple well formats. Each box contains 10 gels. A Mini Gel Tank is required for use of all Bolt gels.

Learn more about our Bolt Bis-Tris Plus gels >

View migration charts ›

Features include:
Optimized run conditions—separate your proteins using constant voltage in approximately 35 minutes
Long shelf life—store gels for at least 16 months at 4–25°C
Better protein integrity—neutral pH sample preparation process preserves your proteins
Wedge-shaped wells—for easier sample loading and up to two times higher sample volume

Choose the right Bolt Bis-Tris Plus gels for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES SDS or Bolt MOPS SDS running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.

Convenient to use
Bolt Bis-Tris Plus gels can be run using constant voltage in approximately 35 minutes with the Bolt MES running buffer. Bolt Bis-Tris Plus gels offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than Tris-glycine–based gels. In addition, Bolt Bis-Tris Plus gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier. And Bolt Bis-Tris Plus gels have a shelf life of at least 16 months at 4°C to 25°C.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer buffer for traditional wet transfer using the Mini Blot Module . Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device .

Novex™ 8-16% Tris-Glycine Mini Gels, WedgeWell™ format, 10-well (Invitrogen™)

Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity. Novex Tris-Glycine Mini Gels are based on traditional Laemmli protein electrophoresis that allow the use of Laemmli sample and running buffers.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine gels, WedgeWell format, include:
Improved shelf life—store gels for up to 6 months at 4°C
Diversity—use for native and denaturing protein assays
Wedge-shaped wells—easily load up to two times more sample volume
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.

Run your proteins in native or denatured form
Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

Convenient to use
Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer when preforming traditional wet transfer using the Invitrogen Mini Blot Module. Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ 12% Bis-Tris Protein Gels, 1.0 mm, 1-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module or XCell II Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

Novex™ 14% Tris-Glycine Mini Gels, WedgeWell™ format, 15-well (Invitrogen™)

Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity. Novex Tris-Glycine Mini Gels are based on traditional Laemmli protein electrophoresis that allow the use of Laemmli sample and running buffers.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine gels, WedgeWell format, include:
Improved shelf life—store gels for up to 6 months at 4°C
Diversity—use for native and denaturing protein assays
Wedge-shaped wells—easily load up to two times more sample volume
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.

Run your proteins in native or denatured form
Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

Convenient to use
Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer when preforming traditional wet transfer using the Invitrogen Mini Blot Module. Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ 6% Tris-Glycine Mini Gels, WedgeWell™ format, 12-well (Invitrogen™)

Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity. Novex Tris-Glycine Mini Gels are based on traditional Laemmli protein electrophoresis that allow the use of Laemmli sample and running buffers.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine gels, WedgeWell format, include:
Improved shelf life—store gels for up to 6 months at 4°C
Diversity—use for native and denaturing protein assays
Wedge-shaped wells—easily load up to two times more sample volume
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.

Run your proteins in native or denatured form
Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

Convenient to use
Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer when preforming traditional wet transfer using the Invitrogen Mini Blot Module. Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ Value™ 10% Tris-Glycine Mini Gels, 1.0 mm, 10-well (Invitrogen™)

Novex™ Value Tris-Glycine Mini Gels provide a great balance of price and performance for your protein separation needs. Novex Value Tris-Glycine polyacrylamide gels provide good separation and resolution of your proteins in an affordable gel that is based on traditional Laemmli protein electrophoresis. Each box contains 20 gels.

Features of the Novex Value gels include:
• Excellent balance of value and performance—great for qualitative analysis (e.g., screening, protein purification verification)
• Fast run conditions—separate your proteins using constant voltage in less than 60 minutes
• Options for native and denaturing protein assays

Choose the right gel format for your experiments
Novex Value Tris-Glycine gels come in a fixed 10% option as well 4–12% and 4–20% gradients.

Run your proteins in native or denatured form
Novex Value Tris-Glycine gels do not contain SDS and can be used to run your proteins in native or denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transferring your proteins to membrane, we recommend using Novex Tris-Glycine Transfer Buffer. Transfers can be done using the Mini Blot Module or the iBlot™ Gel Transfer Device.