Shop All Pre-Cast Protein Gels

Novex™ Value™ 4-20% Tris-Glycine Mini Gels, 1.0 mm, 15-well (Invitrogen™)

Novex™ Value Tris-Glycine Mini Gels provide a great balance of price and performance for your protein separation needs. Novex Value Tris-Glycine polyacrylamide gels provide good separation and resolution of your proteins in an affordable gel that is based on traditional Laemmli protein electrophoresis. Each box contains 20 gels.

Features of the Novex Value gels include:
• Excellent balance of value and performance—great for qualitative analysis (e.g., screening, protein purification verification)
• Fast run conditions—separate your proteins using constant voltage in less than 60 minutes
• Options for native and denaturing protein assays

Choose the right gel format for your experiments
Novex Value Tris-Glycine gels come in a fixed 10% option as well 4–12% and 4–20% gradients.

Run your proteins in native or denatured form
Novex Value Tris-Glycine gels do not contain SDS and can be used to run your proteins in native or denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transferring your proteins to membrane, we recommend using Novex Tris-Glycine Transfer Buffer. Transfers can be done using the Mini Blot Module or the iBlot™ Gel Transfer Device.

NuPAGE™ 10% Bis-Tris Protein Gels, 1.0 mm, 9-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module or XCell II Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

Novex™ 10% Tricine Protein Gels, 1.0 mm, 10-well (Invitrogen™)

Invitrogen Novex Tricine Gels provide separation of low molecular weight proteins and peptides. The Tricine system is a modification of the tris-glycine discontinuous buffer system developed by Schaegger and von Jagow (Schaegger and von Jagow, 1987) specifically for resolving peptides and low molecular weight proteins. In this system the tricine replaces the glycine in the running buffer, resulting in more efficient stacking and destacking for low molecular weight proteins and higher resolution of smaller peptides.

Features of Novex Tricine protein gels:
• Increased resolution of proteins with molecular weights as low as 2 kDa
• Improved compatibility with direct sequencing of proteins after transferring to PVDF
• Minimized protein modification due to the lower pH of the tricine buffering system

Learn more about our Tricine gels ›

View migration charts ›

Formulation: Invitrogen Tricine gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Our Tricine gels have a 4% stacking gel and do not contain SDS. The Tricine system requires SDS in sample and running buffers for best results.

Choose the right Tricine gel for your protein separation
Invitrogen Tricine gels come in three polyacrylamide concentrations of 10%, 16%, and a gradient of 10–20%. Select from our many well formats, including 10-, 12-, and 15-well. Tricine gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, we recommend Tricine SDS Sample Buffer and optimal separation use Tricine SDS Running Buffer.

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer if performing a traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Rapid semi-dry transfer can be performed using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Specialized Protein Gel Support
Protein Gel 1D Electrophoresis Support

NuPAGE™ Tris-Acetate Welcome Pack, 3-8%, 10-well (Invitrogen™)

The NuPAGE Tris-Acetate Welcome Pack provides all of the necessary NuPAGE Tris-Acetate gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The NuPAGE Tris-Acetate Welcome Pack contains:
Mini Gel Tank
• NuPAGE Tris-Acetate mini gels (2 boxes, 20 gels)
NuPAGE Tris-Acetate SDS Running Buffer (20X)
NuPAGE LDS Sample Buffer (4X)
NuPAGE Sample Reducing Agent (10X)
HiMark Pre-stained Protein Standard

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.

About NuPAGE Tris-Acetate gels
NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels.

For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ 4-12% Tris-Glycine Plus Midi Gels 26-well, w/adapters (Invitrogen™)

Novex Tris-Glycine Plus Midi Protein gels are improved polyacrylamide gels based on traditional Laemmli protein electrophoresis technology, allowing the use of Laemmli sample and running buffers. The gels offer reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Plus Midi gels are a 1.0-mm thick, wider format (8 cm x 13 cm) gel for higher throughput electrophoresis of protein samples.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine Plus gels include:
Improved shelf life—store gels for up to 12 months at 4°C
Flexibility—use for native and denaturing protein assays
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine Plus Midi gels come in fixed concentrations of 10% and 12%, as well as gradient concentrations of 4–12%, 4–20%, and 8–16%. Select from our many well formats, including 12+2-, 20-, and 26-well.

Run your proteins in native or denatured form
Novex Tris-Glycine Plus gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer. The gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer when performing traditional wet transfer. Rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ 16% Tris-Glycine Mini Gels, WedgeWell™ format, 15-well (Invitrogen™)

Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity. Novex Tris-Glycine Mini Gels are based on traditional Laemmli protein electrophoresis that allow the use of Laemmli sample and running buffers.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine gels, WedgeWell format, include:
Improved shelf life—store gels for up to 6 months at 4°C
Diversity—use for native and denaturing protein assays
Wedge-shaped wells—easily load up to two times more sample volume
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.

Run your proteins in native or denatured form
Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

Convenient to use
Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer when preforming traditional wet transfer using the Invitrogen Mini Blot Module. Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ Tricine Welcome Pack, 10%, 10-well (Invitrogen™)

The Novex Tricine Welcome Pack provides all of the necessary Novex Tricine gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The Novex Tricine Welcome Pack contains:
Mini Gel Tank
• Novex Tricine Mini Gels (2 boxes, 20 gels)
Novex Tricine SDS Running Buffer (10X)
Novex Tricine SDS Sample Buffer (2X)
NuPAGE Sample Reducing Agent (10X)
Spectra Multicolor Low Range Protein Ladder

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.

About Novex Tricine gels
Novex Tricine gels provide separation of low molecular weight proteins and peptides. The Tricine system is a modification of the tris-glycine discontinuous buffer system developed by Schaegger and von Jagow specifically for peptides and low molecular weight proteins. In this system the tricine replaces the glycine in the running buffer, resulting in more efficient stacking and de-stacking of low molecular weight proteins and higher resolution of smaller peptides.

Transfer
For transfer of proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be performed using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ 8-16% Tris-Glycine Plus Midi Gels 26-well (Invitrogen™)

Novex Tris-Glycine Plus Midi Protein gels are improved polyacrylamide gels based on traditional Laemmli protein electrophoresis technology, allowing the use of Laemmli sample and running buffers. The gels offer reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Plus Midi gels are a 1.0-mm thick, wider format (8 cm x 13 cm) gel for higher throughput electrophoresis of protein samples.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine Plus gels include:
Improved shelf life—store gels for up to 12 months at 4°C
Flexibility—use for native and denaturing protein assays
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine Plus Midi gels come in fixed concentrations of 10% and 12%, as well as gradient concentrations of 4–12%, 4–20%, and 8–16%. Select from our many well formats, including 12+2-, 20-, and 26-well.

Run your proteins in native or denatured form
Novex Tris-Glycine Plus gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer. The gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer when performing traditional wet transfer. Rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ pH 3-10 IEF Protein Gels, 1.0 mm, 10-well (Invitrogen™)

Novex® pH 3–10 IEF Gels are used for pI determination and are excellent for native applications using soluble proteins. Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move in an electric field. Vertical IEF gels effectively create a pH gradient so proteins separate according to their unique pI.

IEG gels can be used to readily detect minor changes in a protein due to deamination, phosphorylation, or glycosylation, and can resolve different proteins of similar size that cannot be resolved on standard SDS-PAGE gels. Pre-focusing is not required. Total run time is approximately 2.5 hours.

Formulation
Novex® IEF gels are 5% polyacrylamide and consist of high-purity acrylamide, bisacrylamide, TEMED, APS, ultrapure water, and 2% ampholytes. They do not contain denaturing reagents.

Recommended buffers
IEF is a sensitive technique which is affected by many factors. Our optimized, premixed IEF buffers reduce variability and help you obtain consistent results.

Novex™ WedgeWell™ Welcome Pack, 8%, 10-well (Invitrogen™)

The Novex Tris-Glycine Welcome Pack provides all of the necessary Novex Tris-glycine gels, buffers, and reagents you need to begin using the Mini Gel Tank.

The Novex Tris-Glycine Welcome Pack contains:
Mini Gel Tank
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
Novex Tris-Glycine SDS Running Buffer (10X)
Novex Tris-Glycine SDS Sample Buffer (2X)
NuPAGE Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.

About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer or Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ 3-8% Tris-Acetate Midi Protein Gels, 20-well (Invitrogen™)

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

Bolt™ Welcome Pack, 12%, 15-well (Invitrogen™)

The Bolt Welcome Pack provides all of the necessary Bolt gels and buffers you will need to begin using the Mini Gel Tank. The Welcome Pack contains:

Mini Gel Tank
• Bolt Bis-Tris Plus gels (2 boxes, 20 gels)
Bolt MES Running Buffer (20X)
Bolt LDS Sample Buffer (4X)
Bolt Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies used.

About Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8, 10, 12, and 4-12% gradient and multiple well formats. Bolt Bis-Tris gels feature wedge-shaped wells, which can easily load up to two times more sample volume allowing for detection of proteins in very dilute samples or visualization of low-abundance proteins.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ WedgeWell™ Welcome Pack, 8%, 15-well (Invitrogen™)

The Novex Tris-Glycine Welcome Pack provides all of the necessary Novex Tris-glycine gels, buffers, and reagents you need to begin using the Mini Gel Tank.

The Novex Tris-Glycine Welcome Pack contains:
Mini Gel Tank
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
Novex Tris-Glycine SDS Running Buffer (10X)
Novex Tris-Glycine SDS Sample Buffer (2X)
NuPAGE Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.

About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer or Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ 3-8% Tris-Acetate Midi Protein Gels, 26-well (Invitrogen™)

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

Bolt™ 4-12% Bis-Tris Plus Gels, 12-well (Invitrogen™)

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Similar to NuPAGE Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications with increased loading capacity. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Use Bolt Bis-Tris Plus gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four gel types and multiple well formats. Each box contains 10 gels. A Mini Gel Tank is required for use of all Bolt gels.

Learn more about our Bolt Bis-Tris Plus gels >

View migration charts ›

Features include:
Optimized run conditions—separate your proteins using constant voltage in approximately 35 minutes
Long shelf life—store gels for at least 16 months at 4–25°C
Better protein integrity—neutral pH sample preparation process preserves your proteins
Wedge-shaped wells—for easier sample loading and up to two times higher sample volume

Choose the right Bolt Bis-Tris Plus gels for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES SDS or Bolt MOPS SDS running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.

Convenient to use
Bolt Bis-Tris Plus gels can be run using constant voltage in approximately 35 minutes with the Bolt MES running buffer. Bolt Bis-Tris Plus gels offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than Tris-glycine–based gels. In addition, Bolt Bis-Tris Plus gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier. And Bolt Bis-Tris Plus gels have a shelf life of at least 16 months at 4°C to 25°C.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer buffer for traditional wet transfer using the Mini Blot Module . Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device .