Shop All Tris-Acetate Protein Gels

NuPAGE™ Tris-Acetate Welcome Pack, 3-8%, 12-well (Invitrogen™)

The NuPAGE Tris-Acetate Welcome Pack provides all of the necessary NuPAGE Tris-Acetate gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The NuPAGE Tris-Acetate Welcome Pack contains:
Mini Gel Tank
• NuPAGE Tris-Acetate mini gels (2 boxes, 20 gels)
NuPAGE Tris-Acetate SDS Running Buffer (20X)
NuPAGE LDS Sample Buffer (4X)
NuPAGE Sample Reducing Agent (10X)
HiMark Pre-stained Protein Standard

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.

About NuPAGE Tris-Acetate gels
NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels.

For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ 3-8% Tris-Acetate Midi Protein Gels, 12+2-well (Invitrogen™)

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ 3-8% Tris-Acetate Protein Gels, 1.0 mm, 10-well (Invitrogen™)

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ Tris-Acetate Welcome Pack, 7%, 10-well (Invitrogen™)

The NuPAGE Tris-Acetate Welcome Pack provides all of the necessary NuPAGE Tris-Acetate gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The NuPAGE Tris-Acetate Welcome Pack contains:
Mini Gel Tank
• NuPAGE Tris-Acetate mini gels (2 boxes, 20 gels)
NuPAGE Tris-Acetate SDS Running Buffer (20X)
NuPAGE LDS Sample Buffer (4X)
NuPAGE Sample Reducing Agent (10X)
HiMark Pre-stained Protein Standard

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.

About NuPAGE Tris-Acetate gels
NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels.

For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ Large Protein Sensitive Staining Kit (Invitrogen™)

The HiMark™ Pre-Stained and Unstained High Molecular Weight Protein Standards are specifically designed for large protein analysis on NuPAGE® Novex Tris-Acetate Gels under denaturing conditions. Both standards offer a time-saving load-and-go format.

The HiMark™ Pre-stained Standard includes one pink colored orientation band and eight blue bands ranging in apparent molecular weight of 31 - 460 kDa* in NuPAGE® Tris-Acetate Gel/SDS buffer system (Figure 1). It's suitable for monitoring electrophoresis, western transfer, and approximating molecular weight of unknown big proteins.

The HiMark Unstained Standard, along with the NuPAGE® Tris-Acetate Gel/SDS buffer system, offers the highest accuracy for molecular weight estimation of large proteins. It consists of nine proteins, ranging in size from 40 to 500 kDa in NuPAGE® Novex 3-8% and 7% Tris-Acetate Gel/SDS buffer systems (Figure 2). Visualize with Coomassie® stain, silver, and fluorescent protein stains post-electrophoresis. A downloadable molecular weight calculator is designed for easy and accurate MW estimation of large proteins using the HiMark™ Unstained Standard on a NuPAGE® Tris-Acetate Gel.**

Several NuPAGE® Large Protein Starter Kits are available to provide you with all necessary reagents and optimized protocols for highresolution large protein separation and analysis (Table 1). Each kit includes two boxes of NuPAGE® 3-8% Tris-Acetate Gels, Tris-Acetate SDS Buffer Kit, a staining reagent or blotting membranes, and a vial of the appropriate HiMark™ Standard.

NuPAGE™ 7% Tris-Acetate Protein Gels, 1.0 mm, 12-well (Invitrogen™)

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ 3-8% Tris-Acetate Midi Protein Gels, 20-well, w/adapters (Invitrogen™)

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ 3-8% Tris-Acetate Protein Gels, 1.5 mm, 15-well (Invitrogen™)

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ 3-8% Tris-Acetate Midi Protein Gels, 26-well, w/adapters (Invitrogen™)

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ Tris-Acetate Welcome Pack, 3-8%, 15-well (Invitrogen™)

The NuPAGE Tris-Acetate Welcome Pack provides all of the necessary NuPAGE Tris-Acetate gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The NuPAGE Tris-Acetate Welcome Pack contains:
Mini Gel Tank
• NuPAGE Tris-Acetate mini gels (2 boxes, 20 gels)
NuPAGE Tris-Acetate SDS Running Buffer (20X)
NuPAGE LDS Sample Buffer (4X)
NuPAGE Sample Reducing Agent (10X)
HiMark Pre-stained Protein Standard

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.

About NuPAGE Tris-Acetate gels
NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels.

For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ 3-8% Tris-Acetate Protein Gels, 1.0 mm, 12-well (Invitrogen™)

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ Tris-Acetate Welcome Pack, 7%, 12-well (Invitrogen™)

The NuPAGE Tris-Acetate Welcome Pack provides all of the necessary NuPAGE Tris-Acetate gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The NuPAGE Tris-Acetate Welcome Pack contains:
Mini Gel Tank
• NuPAGE Tris-Acetate mini gels (2 boxes, 20 gels)
NuPAGE Tris-Acetate SDS Running Buffer (20X)
NuPAGE LDS Sample Buffer (4X)
NuPAGE Sample Reducing Agent (10X)
HiMark Pre-stained Protein Standard

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.

About NuPAGE Tris-Acetate gels
NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels.

For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ Tris-Acetate Welcome Pack, 3-8%, 10-well (Invitrogen™)

The NuPAGE Tris-Acetate Welcome Pack provides all of the necessary NuPAGE Tris-Acetate gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The NuPAGE Tris-Acetate Welcome Pack contains:
Mini Gel Tank
• NuPAGE Tris-Acetate mini gels (2 boxes, 20 gels)
NuPAGE Tris-Acetate SDS Running Buffer (20X)
NuPAGE LDS Sample Buffer (4X)
NuPAGE Sample Reducing Agent (10X)
HiMark Pre-stained Protein Standard

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.

About NuPAGE Tris-Acetate gels
NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels.

For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ 3-8% Tris-Acetate Midi Protein Gels, 12+2-well, w/adapters (Invitrogen™)

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ 7% Tris-Acetate Protein Gels, 1.0 mm, 15-well (Invitrogen™)

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis