Shop All Bis-Tris Protein Gels

NuPAGE™ 10% Bis-Tris Protein Gels, 1.0 mm, 10-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module or XCell II Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ 4-12% Bis-Tris Midi Protein Gels, 12+2-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

NuPAGE Bis-Tris Midi gels can be used with the XCell SureLock Midi Cell gel apparatuses or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the Bio-Rad Criterion Cell using NuPAGE Transfer buffer.

Related links
Overview of 1D Protein Electrophoresis

Bolt™ 10% Bis-Tris Plus Gels, 15-well (Invitrogen™)

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Similar to NuPAGE Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications with increased loading capacity. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Use Bolt Bis-Tris Plus gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four gel types and multiple well formats. Each box contains 10 gels. A Mini Gel Tank is required for use of all Bolt gels.

Learn more about our Bolt Bis-Tris Plus gels >

View migration charts ›

Features include:
Optimized run conditions—separate your proteins using constant voltage in approximately 35 minutes
Long shelf life—store gels for at least 16 months at 4–25°C
Better protein integrity—neutral pH sample preparation process preserves your proteins
Wedge-shaped wells—for easier sample loading and up to two times higher sample volume

Choose the right Bolt Bis-Tris Plus gels for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES SDS or Bolt MOPS SDS running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.

Convenient to use
Bolt Bis-Tris Plus gels can be run using constant voltage in approximately 35 minutes with the Bolt MES running buffer. Bolt Bis-Tris Plus gels offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than Tris-glycine–based gels. In addition, Bolt Bis-Tris Plus gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier. And Bolt Bis-Tris Plus gels have a shelf life of at least 16 months at 4°C to 25°C.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer buffer for traditional wet transfer using the Mini Blot Module . Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device .

NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0 mm, 17-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module or XCell II Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

Bolt™ Welcome Pack, 12%, 10-well (Invitrogen™)

The Bolt Welcome Pack provides all of the necessary Bolt gels and buffers you will need to begin using the Mini Gel Tank. The Welcome Pack contains:

Mini Gel Tank
• Bolt Bis-Tris Plus gels (2 boxes, 20 gels)
Bolt MES Running Buffer (20X)
Bolt LDS Sample Buffer (4X)
Bolt Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies used.

About Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8, 10, 12, and 4-12% gradient and multiple well formats. Bolt Bis-Tris gels feature wedge-shaped wells, which can easily load up to two times more sample volume allowing for detection of proteins in very dilute samples or visualization of low-abundance proteins.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0 mm, 12-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use.

Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Learn more about all of our NuPAGE Bis-Tris gels >
View migration charts ›

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

We also provide NuPAGE Tris-Acetate gels for separating larger proteins. For classic Laemmli-based Tris-glycine electrophoresis, we provide Novex Tris-Glycine gels.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the XCell II Blot Module or the Mini Blot Module.

Related links
Overview of 1D Protein Electrophoresis
Comparison of NuPAGE Tris-Bis vs. traditional Tris-glycine gels

NuPAGE™ 4-12% Bis-Tris Midi Protein Gels, 20-well, w/adapters (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

NuPAGE Bis-Tris Midi gels can be used with the XCell SureLock Midi Cell gel apparatuses or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the Bio-Rad Criterion Cell using NuPAGE Transfer buffer.

Related links
Overview of 1D Protein Electrophoresis

Bolt™ 10% Bis-Tris Plus Gels, 17-well (Invitrogen™)

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Similar to NuPAGE Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications with increased loading capacity. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Use Bolt Bis-Tris Plus gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four gel types and multiple well formats. Each box contains 10 gels. A Mini Gel Tank is required for use of all Bolt gels.

Learn more about our Bolt Bis-Tris Plus gels >

View migration charts ›

Features include:
Optimized run conditions—separate your proteins using constant voltage in approximately 35 minutes
Long shelf life—store gels for at least 16 months at 4–25°C
Better protein integrity—neutral pH sample preparation process preserves your proteins
Wedge-shaped wells—for easier sample loading and up to two times higher sample volume

Choose the right Bolt Bis-Tris Plus gels for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES SDS or Bolt MOPS SDS running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.

Convenient to use
Bolt Bis-Tris Plus gels can be run using constant voltage in approximately 35 minutes with the Bolt MES running buffer. Bolt Bis-Tris Plus gels offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than Tris-glycine–based gels. In addition, Bolt Bis-Tris Plus gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier. And Bolt Bis-Tris Plus gels have a shelf life of at least 16 months at 4°C to 25°C.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer buffer for traditional wet transfer using the Mini Blot Module . Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device .

NuPAGE™ 10% Bis-Tris Midi Protein Gels, 12+2-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

NuPAGE Bis-Tris Midi gels can be used with the XCell SureLock Midi Cell gel apparatuses or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the Bio-Rad Criterion Cell using NuPAGE Transfer buffer.

Related links
Overview of 1D Protein Electrophoresis

Bolt™ Welcome Pack, 12%, 12-well (Invitrogen™)

The Bolt Welcome Pack provides all of the necessary Bolt gels and buffers you will need to begin using the Mini Gel Tank. The Welcome Pack contains:

Mini Gel Tank
• Bolt Bis-Tris Plus gels (2 boxes, 20 gels)
Bolt MES Running Buffer (20X)
Bolt LDS Sample Buffer (4X)
Bolt Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies used.

About Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8, 10, 12, and 4-12% gradient and multiple well formats. Bolt Bis-Tris gels feature wedge-shaped wells, which can easily load up to two times more sample volume allowing for detection of proteins in very dilute samples or visualization of low-abundance proteins.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NuPAGE™ 10% Bis-Tris Protein Gels, 1.0 mm, 12-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module or XCell II Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

Bolt™ 4-12% Bis-Tris Plus Gels, 12-well (Invitrogen™)

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Similar to NuPAGE Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications with increased loading capacity. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Use Bolt Bis-Tris Plus gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four gel types and multiple well formats. Each box contains 10 gels. A Mini Gel Tank is required for use of all Bolt gels.

Learn more about our Bolt Bis-Tris Plus gels >

View migration charts ›

Features include:
Optimized run conditions—separate your proteins using constant voltage in approximately 35 minutes
Long shelf life—store gels for at least 16 months at 4–25°C
Better protein integrity—neutral pH sample preparation process preserves your proteins
Wedge-shaped wells—for easier sample loading and up to two times higher sample volume

Choose the right Bolt Bis-Tris Plus gels for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES SDS or Bolt MOPS SDS running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.

Convenient to use
Bolt Bis-Tris Plus gels can be run using constant voltage in approximately 35 minutes with the Bolt MES running buffer. Bolt Bis-Tris Plus gels offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than Tris-glycine–based gels. In addition, Bolt Bis-Tris Plus gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier. And Bolt Bis-Tris Plus gels have a shelf life of at least 16 months at 4°C to 25°C.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer buffer for traditional wet transfer using the Mini Blot Module . Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device .

NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0 mm, 15-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module or XCell II Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

NuPAGE™ 10% Bis-Tris Protein Gels, 1.0 mm, 1-well (Invitrogen™)

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module or XCell II Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

Bolt™ 8% Bis-Tris Plus Gels, 17-well (Invitrogen™)

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Similar to NuPAGE Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications with increased loading capacity. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Use Bolt Bis-Tris Plus gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four gel types and multiple well formats. Each box contains 10 gels. A Mini Gel Tank is required for use of all Bolt gels.

Learn more about our Bolt Bis-Tris Plus gels >

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Features include:
Optimized run conditions—separate your proteins using constant voltage in approximately 35 minutes
Long shelf life—store gels for at least 16 months at 4–25°C
Better protein integrity—neutral pH sample preparation process preserves your proteins
Wedge-shaped wells—for easier sample loading and up to two times higher sample volume

Choose the right Bolt Bis-Tris Plus gels for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES SDS or Bolt MOPS SDS running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.

Convenient to use
Bolt Bis-Tris Plus gels can be run using constant voltage in approximately 35 minutes with the Bolt MES running buffer. Bolt Bis-Tris Plus gels offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than Tris-glycine–based gels. In addition, Bolt Bis-Tris Plus gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier. And Bolt Bis-Tris Plus gels have a shelf life of at least 16 months at 4°C to 25°C.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer buffer for traditional wet transfer using the Mini Blot Module . Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device .