Shop All Blotting Membranes

PVDF Transfer Membranes, 0.45 μm, 10 x 10 cm Thermo Scientific™

Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for western, southern, and northern blotting methods. The 0.45-µm pore size makes these membranes ideal for protein transfer for proteins >10 kDa.

See all membranes ›

Features
Membrane: polyvinylidene difluoride (PVDF)
Binding capacity: goat IgG: 294 µg/cm2; BSA: 215 µg/cm2; insulin: 160 µg/cm2
Re-probe characteristics: yes
Pre-activation: required with 100% alcohol (methanol, ethanol, or isopropanol)
• Compatibility*: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
Durability: compatible with most organic solvents, acids, and mild bases

*For fluorescent applications use low-fluorescent PVDF membranes.

Nitrocellulose/Filter Paper Sandwiches, 0.45 μm, 8.5 x 13.5 cm Invitrogen™

Nitrocellulose/Filter Paper Sandwiches, 0.45 µm, are high-quality membranes ideal for blotting proteins and nucleic acids. The 0.45-µm pore size is ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp). The included extra-thick absorbent blotting paper (2.5 mm) accommodates blotting set-up using semi-dry transfer apparatuses.

See all membranes and filter papers ›

Features:
Membrane: 100% pure nitrocellulose
Filter paper thickness: 2.5 mm
Binding capacity: ~80 µg/cm2 of proteins
Binding interactions: hydrophobic and electrostatic
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• Supplied in a pre-cut, pre-assembled membrane/filter paper sandwich

iBlot™ Transfer Stack, PVDF, regular size Invitrogen™

The iBlot® Transfer Stacks are used to transfer proteins using the iBlot® Gel Transfer Device. The iBlot® Transfer Stacks are disposable stacks that come in three varieties for different applications: iBlot® Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins, iBlot® DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA, and iBlot® Western Detection Stacks are used to perform Western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot® Transfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.

PVDF membrane
The PVDF membrane (0.2 µm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. The PVDF membrane is preactivated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein binding capacity is 240 µg/cm2.

Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex® midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex® mini-gels (or equivalent).

Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 µm, 8.5 x 13.5 cm Invitrogen™

Invitrolon PVDF/Filter Paper Sandwiches, 0.45 µm, have excellent binding properties for western blotting, dot-blot assays, and protein sequencing. The 0.45-µm pore size makes these membranes ideal for protein transfer for proteins >10 kDa. The included extra-thick absorbent blotting paper (2.5 mm) accommodates blotting set-up using semi-dry transfer apparatuses.

See all membranes and filter papers ›

Features
Membrane: polyvinylidene difluoride (PVDF)
Filter paper thickness: 2.5 mm
Binding capacity: goat IgG: 294 µg/cm2; BSA: 131 µg/cm2; insulin: 85 µg/cm2
Re-probe characteristics: yes
Pre-activation: required with 100% alcohol (methanol)
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
Durability: compatible with most organic solvents, acids, and mild bases

iBlot™ Transfer Stack, PVDF, mini Invitrogen™

The iBlot® Transfer Stacks are used to transfer proteins using the iBlot® Gel Transfer Device. The iBlot® Transfer Stacks are disposable stacks that come in three varieties for different applications: iBlot® Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins, iBlot® DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA, and iBlot® Western Detection Stacks are used to perform Western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot® Transfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.

PVDF membrane
The PVDF membrane (0.2 µm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. The PVDF membrane is preactivated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein binding capacity is 240 µg/cm2.

Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex® midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex® mini-gels (or equivalent).

Blotting Filter Papers, 2.5-mm thick, 8.6 x 13.5 cm Invitrogen™

Blotting Filter Papers, 2.5-mm thick, are pre-cut, extra-thick, absorbent blotting papers for use in semi-dry transfers. The 8.6 cm x 13.5-cm size accommodates one midi-size gel, one E-PAGE gel, or two mini-size gels.

See all filter papers ›

Western Blotting Filter Papers, 0.83-mm Thick, 8 x 13.5 cm Thermo Scientific™

Pierce Western Blotting Filter Papers, 0.83-mm thick, are pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes.

See all filter papers ›

Pierce Western Blotting Filter Papers are composed of high-quality alpha cotton cellulose fiber for use in northern, southern, and western blotting applications. These filter papers help to ensure quick and efficient transfer of molecules from gel to membrane. They are manufactured with ultrapure water without additives to minimize possible sources of background signal. They are suitable for use with alcohol or other organic solvents commonly used in protein and nucleic acid blotting applications.

iBlot™ 2 Transfer Stacks, nitrocellulose, regular size Invitrogen™

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacks with an integrated nitrocellulose transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This regular-size stack (13 cm x 8.3 cm) can be used with midi-size gels or two mini-size gels or E-PAGE 48 or 96 gels.

Learn more about the iBlot 2 system ›

Nitrocellulose membrane
The nitrocellulose membrane (0.2-µm pore size) in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein-binding capacity is 209 µg/cm2.

Nitrocellulose/Filter Paper Sandwiches, 0.2 μm, 8.5 x 13.5 cm Invitrogen™

Nitrocellulose/Filter Paper Sandwiches, 0.2 µm, are high-quality membranes ideal for blotting proteins and nucleic acids. The 0.2-µm pore size is ideal for the transfer of low molecular weight proteins (<20 kDa) or peptides and nucleic acids (<300 bp). The included extra-thick, absorbent blotting paper (2.5 mm) accommodates blotting set-up using semi-dry transfer apparatuses.

See all membranes and filter papers ›

Features:
Membrane: 100% pure nitrocellulose
Filter paper thickness: 2.5 mm
Binding capacity: ~80 µg/cm2 of proteins
Binding interactions: hydrophobic and electrostatic
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• Supplied in a pre-cut, pre-assembled membrane/filter paper sandwich

iBlot™ DNA Transfer Stack Invitrogen™

The iBlot® Dry Blotting System is a quick and efficient system for transferring DNA from agarose or polyacrylamide gels in seven minutes. With dry blotting you don’t need additional buffers or liquids as you do with capillary, vacuum, or electro- blotting—which all adds up to less variability in your results. Get iBlot® Dry Blotting System and enjoy:
• Time savings—transfer DNA in 7 minutes instead of 16 hours
• Reproducibility—consistent blot preparation and running conditions
• Convenience—avoid messy buffers and laborious prep work The iBlot® Dry Blotting System consists of a Gel Transfer Device and Transfer Stacks. The iBlot® Transfer Stacks are pre-assembled and intended for single use only.

iBlot™ 2 Transfer Stacks, nitrocellulose, mini Invitrogen™

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacks with an integrated nitrocellulose transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This mini-size stack (8 cm x 8 cm) can be used with one mini-size gel.

Learn more about the iBlot 2 system ›

Nitrocellulose membrane
The nitrocellulose membrane (0.2-µm pore size) in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein-binding capacity is 209 µg/cm2.

Blotting Filter Papers, 2.5-mm thick, 7.5 x 8.4 cm Invitrogen™

Blotting Filter Papers, 2.5-mm thick, are pre-cut, extra-thick, absorbent blotting paper for use in semi-dry transfers. The 7.5-cm x 8.4-cm size accommodates most mini-size hand-cast or pre-cast gels.

See all filter papers ›

Power Blotter Pre-cut Membranes and Filters, nitrocellulose, mini Invitrogen™

These Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for transfer of proteins from mini-size gels using the Power Blotter System. The pre-cut membranes and filters include 20 sheets of nitrocellulose and 80 sheets of absorbent blotting paper (0.85 mm thickness). Power Blotter Pre-cut Membranes and Filters combined with Power Blotter 1-Step Transfer Buffer allow for high-efficiency semi-dry transfer of proteins in less than 10 minutes.

Features of nitrocellulose membranes:
High-quality—pure 100% nitrocellulose membranes with high surface area and excellent uniformity
High-binding affinity—provides excellent protein binding (209 µg/cm2), blocks easily, and produces very low background in chemiluminescent and fluorescent western blotting
Convenient—pre-cut nitrocellulose sheets are ready to use for transfer and blotting experiments from standard precast or homemade mini gels (8 cm x 8 cm); no need to cut your own membranes, minimizing damage and contamination
Validated—membrane and filter when combined provide the thickness and buffer capacity needed for optimal performance in the Power Blotter System

The nitrocellulose membrane (0.45 µM pore size) is composed of 100% pure nitrocellulose to provide high-quality transfers. Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (e.g., chemiluminescence, chromogenic, fluorescence). These pre-cut membrane/filter paper sandwiches fit mini sized gels.

Learn more about the Power Blotter System ›

Related products
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size
Power Blotter Pre-cut Membranes and Filters, PVDF, mini
Power Blotter Pre-cut Membranes and Filters, PVDF, regular size

Power Blotter Select Transfer Stacks, nitrocellulose, regular size Invitrogen™

The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. They are disposable, pre-assembled stacks that come with an integrated nitrocellulose transfer membrane for the dry blotting of proteins from polyacrylamide gels. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins.

Features of the Power Blotter Select Transfer Stacks:
Rapid transfer—the ready-to-use transfer stack takes little setup time and protein transfers take place in as little as seven minutes
Efficient and even transfer—stacks are made with high quality membranes that provide high binding efficiency, uniform transfer, and high detection sensitivity
Increased reproducibility—preassembled stacks provide consistent performance

Learn more about the use of the Power Blotter Select Transfer Stacks in Power Blotter System

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) in the stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, chemiluminescence, and fluorescence. The proteins bind to the membrane through hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Regular-size transfer stacks
Regular-size Power Blotter Select Stacks (13 cm x 8.3 cm) can be used with all Invitrogen Novex and NuPAGE midi-gels (or equivalent) or two mini-size gels (all Novex, Bolt, and NuPAGE mini-size gels, or equivalent).

Related products
Power Blotter Select Transfer Stacks, nitrocellulose, mini size
Power Blotter Select Transfer Stacks, PVDF, mini
Power Blotter Select Transfer Stacks, PVDF, regular size

Western Blotting Filter Papers, 0.83-mm Thick, 8 x 13.5 cm Thermo Scientific™

Pierce Western Blotting Filter Papers, 2.5-mm thick, are pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes.

See all filter papers ›

Pierce Western Blotting Filter Papers are composed of high-quality alpha cotton cellulose fiber for use in northern, southern, and western blotting applications. These filter papers help to ensure quick and efficient transfer of molecules from gel to membrane. They are manufactured with ultrapure water without additives to minimize possible sources of background signal. They are suitable for use with alcohol or other organic solvents commonly used in protein and nucleic acid blotting applications.

Results per page
    spinner