Shop All Blotting Membranes

Power Blotter Select Transfer Stacks, nitrocellulose, mini (Invitrogen™)

The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. They are disposable, pre-assembled stacks that come with an integrated nitrocellulose transfer membrane for the dry blotting of proteins from polyacrylamide gels. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins.

Features of the Power Blotter Select Transfer Stacks:
Rapid transfer—the ready-to-use transfer stack takes little setup time with protein transfer taking place in as little as seven minutes
Efficient and even transfer—stacks are made with high-quality membranes that provide high binding efficiency, uniform transfer, and high detection sensitivity
Increased reproducibility—preassembled stacks provide consistent performance

Learn more about the use of the Power Blotter Select Transfer Stacks in Power Blotter System

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) in the stacks is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, chemiluminescence, and fluorescence. The proteins bind to the membrane through hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Mini transfer stacks
The mini-size (8 cm x 8 cm) Power Blotter Select Stacks can be used with all Invitrogen Novex, Bolt, and NuPAGE mini-size gels or equivalent.

Related products
Power Blotter Select Transfer Stacks, nitrocellulose, regular size
Power Blotter Select Transfer Stacks, PVDF, mini
Power Blotter Select Transfer Stacks, PVDF, regular size

iBlot™ 2 Transfer Stacks, PVDF, mini (Invitrogen™)

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacks with an integrated pre-activated PVDF transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This mini-size stack (8 cm x 8 cm) can be used with one mini-size gel.

Learn more about the iBlot 2 system ›

PVDF membrane
The PVDF membrane (0.2-µm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. It is pre-activated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein-binding capacity is 240 µg/cm2.

Western Blotting Filter Paper, 0.83 mm thick, 7 x 8.4 cm (Thermo Scientific™)

Pierce Western Blotting Filter Papers, 0.83-mm thick, are pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes. The 7 x 8.4-cm size accommodates most mini-size hand-cast or pre-cast gels.

See all filter papers ›

Pierce Western Blotting Filter Papers are composed of high-quality alpha cotton cellulose fiber for use in northern, southern, and western blotting applications. These filter papers help to ensure quick and efficient transfer of molecules from gel to membrane. They are manufactured with ultrapure water without additives to minimize possible sources of background signal. They are suitable for use with alcohol or other organic solvents commonly used in protein and nucleic acid blotting applications.

Power Blotter Select Transfer Stacks, nitrocellulose, regular size (Invitrogen™)

The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. They are disposable, pre-assembled stacks that come with an integrated nitrocellulose transfer membrane for the dry blotting of proteins from polyacrylamide gels. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins.

Features of the Power Blotter Select Transfer Stacks:
Rapid transfer—the ready-to-use transfer stack takes little setup time and protein transfers take place in as little as seven minutes
Efficient and even transfer—stacks are made with high quality membranes that provide high binding efficiency, uniform transfer, and high detection sensitivity
Increased reproducibility—preassembled stacks provide consistent performance

Learn more about the use of the Power Blotter Select Transfer Stacks in Power Blotter System

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) in the stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, chemiluminescence, and fluorescence. The proteins bind to the membrane through hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Regular-size transfer stacks
Regular-size Power Blotter Select Stacks (13 cm x 8.3 cm) can be used with all Invitrogen Novex and NuPAGE midi-gels (or equivalent) or two mini-size gels (all Novex, Bolt, and NuPAGE mini-size gels, or equivalent).

Related products
Power Blotter Select Transfer Stacks, nitrocellulose, mini size
Power Blotter Select Transfer Stacks, PVDF, mini
Power Blotter Select Transfer Stacks, PVDF, regular size

PVDF Transfer Membranes, 0.45 µm, 10 x 10 cm (Thermo Scientific™)

Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for western, southern, and northern blotting methods. The 0.45-µm pore size makes these membranes ideal for protein transfer for proteins >10 kDa.

See all membranes ›

Features
Membrane: polyvinylidene difluoride (PVDF)
Binding capacity: goat IgG: 294 µg/cm2; BSA: 215 µg/cm2; insulin: 160 µg/cm2
Re-probe characteristics: yes
Pre-activation: required with 100% alcohol (methanol, ethanol, or isopropanol)
• Compatibility*: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
Durability: compatible with most organic solvents, acids, and mild bases

*For fluorescent applications use low-fluorescent PVDF membranes.

BrightStar™-Plus Positively Charged Nylon Membrane, 15 cm x 15 cm (Invitrogen™)

Ambion® BrightStar®-Plus Positively Charged Nylon Membrane(s) are strongly recommended to ensure consistent, reliable results with minimal background. Ideal for use with radiolabeled and nonisotopic probes for northern and other blot applications, five sheets measuring 15 x 15 cm are provided. This membrane exhibits the lowest nonspecific binding in nonisotopic detection applications when used with Ambion® blocking and wash solutions.

iBlot™ Transfer Stack, nitrocellulose, regular size (Invitrogen™)

The iBlot® Transfer Stacks are used to transfer proteins using the iBlot® Gel Transfer Device. The iBlot® Transfer Stacks are disposable stacks that come in three varieties for different applications: iBlot® Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins, iBlot® DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA, and iBlot® Western Detection Stacks are used to perform Western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot® Transfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex® midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex® mini-gels (or equivalent).

Western Blotting Filter Paper, 0.83 mm thick, 8 x 13.5 cm (Thermo Scientific™)

Pierce Western Blotting Filter Papers, 0.83-mm thick, are pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes. The 8 x 13.5-cm size accommodates one midi-size gel or two mini-size gels.

See all filter papers ›

Pierce Western Blotting Filter Papers are composed of high-quality alpha cotton cellulose fiber for use in northern, southern, and western blotting applications. These filter papers help to ensure quick and efficient transfer of molecules from gel to membrane. They are manufactured with ultrapure water without additives to minimize possible sources of background signal. They are suitable for use with alcohol or other organic solvents commonly used in protein and nucleic acid blotting applications.

iBlot™ Transfer Stack, PVDF, regular size (Invitrogen™)

The iBlot® Transfer Stacks are used to transfer proteins using the iBlot® Gel Transfer Device. The iBlot® Transfer Stacks are disposable stacks that come in three varieties for different applications: iBlot® Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins, iBlot® DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA, and iBlot® Western Detection Stacks are used to perform Western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot® Transfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.

PVDF membrane
The PVDF membrane (0.2 µm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. The PVDF membrane is preactivated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein binding capacity is 240 µg/cm2.

Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex® midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex® mini-gels (or equivalent).

Blotting Filter Papers, 2.5-mm thick, 8.6 x 13.5 cm (Invitrogen™)

Blotting Filter Papers, 2.5-mm thick, are pre-cut, extra-thick, absorbent blotting papers for use in semi-dry transfers. The 8.6 cm x 13.5-cm size accommodates one midi-size gel, one E-PAGE gel, or two mini-size gels.

See all filter papers ›

BrightStar™-Plus Positively Charged Nylon Membrane, 30 cm x 45 cm (Invitrogen™)

Ambion® BrightStar®-Plus Positively Charged Nylon Membrane(s) are strongly recommended to ensure consistent, reliable results with minimal background. Ideal for use with radiolabeled and nonisotopic probes for northern and other blot applications; one roll of 30 x 45 cm is provided. This membrane exhibits the lowest nonspecific binding in nonisotopic detection applications when used with Ambion® blocking and wash solutions.

iBlot™ Transfer Stack, nitrocellulose, mini (Invitrogen™)

iBlot® Transfer Stacks are used to transfer proteins using the iBlot® Gel Transfer Device. The iBlot® Transfer Stacks are disposable stacks that come in three varieties for different applications. iBlot® Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins. iBlot® DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA. iBlot® Western Detection Stacks are used to perform western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot® Transfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. Proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex® midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex® mini-gels (or equivalent).

Power Blotter Select Transfer Stacks, PVDF, mini (Invitrogen™)

The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. They are disposable, pre-assembled stacks that come with an integrated PVDF transfer membrane for dry blotting of proteins from polyacrylamide gels. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins.

Features of the Power Blotter Select Transfer Stacks:
Rapid transfer—the ready-to-use transfer stack takes little setup time and protein transfers take place in as little as seven minutes
Efficient and even transfer—stacks are made with high-quality membranes that provide high binding efficiency, uniform transfer, and high detection sensitivity
Increased reproducibility—preassembled stacks provide consistent performance

Learn more about the use of the Power Blotter Select Transfer Stacks in Power Blotter System

PVDF membrane
The PVDF membrane (0.2 µm pore size, low fluorescence) in the stack has higher binding capacity than nitrocellulose. It is pre-activated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane through hydrophobic interactions. The protein-binding capacity is 240 µg/cm2.

Mini transfer stacks
The mini-size (8 cm x 8 cm) Power Blotter Select Stacks can be used with all Invitrogen Novex, Bolt, and NuPAGE mini-size gels or equivalent.

Related products
Power Blotter Select Transfer Stacks, PVDF, regular size
Power Blotter Select Transfer Stacks, nitrocellulose, mini size
Power Blotter Select Transfer Stacks, nitrocellulose, regular size

Biodyne™ B Nylon Membrane, 0.45 µm, 8 cm x 12 cm (Thermo Scientific™)

Thermo Scientific Biodyne B Nylon Membranes are sheets of positively charged nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods.

Features of Biodyne B Nylon Membranes:

Versatile and durable—achieve strong DNA binding by baking or UV-crosslinking without damaging the membrane
Easy to use—pre-cut 8 x 12 cm sheets are the ideal size for standard mini-gel transfers and microplate dot-blots
High signal-to-noise ratio—lower background than nitrocellulose and most other nylon transfer membranes

Biodyne B Nylon Membrane is manufactured with a matrix pore size of 0.4 micron, and is supplied in packages of 8 x 12 cm sheets. Biodyne B Membrane contains exclusively positive charges, providing the highest possible binding capacity for negatively charged molecules like DNA and RNA. The membrane is resistant to heat and solvents and will not shrink, crack or tear.

Properties of Biodyne B Nylon Membrane:
• Composition: positively charged Nylon 66
• Surface: strong cationic quaternary ammonium groups provide positive zeta potential, even to pH 10
• Properties: excellent for DNA, RNA and other negatively charged molecules
• Applications: Southern blotting, Northern blotting, DNA and RNA methods, including gel shift assays with Thermo Scientific LightShift Chemiluminescent EMSA Kits

More Product Data
Transfer EMSA gels using the Pierce G2 Fast Blotter

Related Products
Biodyne™ A Nylon Membrane, 0.45 µm, 8 cm x 12 cm

Nitrocellulose/Filter Paper Sandwich, 0.2 µm, 8.3 x 7.3 cm (Invitrogen™)

Nitrocellulose/Filter Paper Sandwiches, 0.2 µm, are high-quality membranes ideal for blotting proteins and nucleic acids. The 0.2-µm pore size is ideal for the transfer of low molecular weight proteins (<20 kDa) and nucleic acids (<300 bp).

See all membranes and filter papers ›

Features:
Membrane: 100% pure nitrocellulose
Filter paper thickness: 0.8 mm
Binding capacity: ~209 µg/cm2 of proteins
Binding interactions: hydrophobic and electrostatic
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• Supplied in a pre-cut, pre-assembled membrane/filter paper sandwich