Shop All Blotting Membranes

Nitrocellulose Membrane, 0.45 µm, 7.9 cm x 10.5 cm (Thermo Scientific™)

These Thermo Scientific Pierce Nitrocellulose Membranes are 0.45 µM, 7.9 cm x 10.5 cm sheets of nitrocellulose that have excellent binding properties for western blotting, dot-blot assays and other protein or nucleic acid methods.

Features of Nitrocellulose Membranes:

High-quality—pure, 100% nitrocellulose membranes with high surface area and excellent uniformity
Protein applications—0.45 µM pore size
Convenient—7.9 cm x 10.5 cm pre-cut sheets
High-binding affinity—provides excellent protein binding, blocks easily, and produces very low background in chemiluminescent Western blotting

Nitrocellulose is a popular binding matrix for Western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (e.g., chemiluminescence, chromogenic, fluorescence).

Related Products
Nitrocellulose Membrane, 0.45 µm, 30 cm x 3.5 m
Nitrocellulose Membrane, 0.45 µm, 8 cm x 12 cm
Nitrocellulose Membrane, 0.45 µm, 8 cm x 8 cm

PVDF/Filter Paper Sandwich, 0.2 µm, 8.3 x 7.3 cm (Invitrogen™)

PVDF/Filter Paper Sandwiches, 0.2 µm, have excellent binding properties for western blotting, dot-blot assays, and other protein or nucleic acid methods such as protein sequencing. The 0.2-µm pore size makes these membranes ideal for protein analysis of small amounts of proteins (as little as 10 pmoles), amino acid analysis, and transfer of small molecular weight proteins and peptides.

See all membranes and filter papers ›

Features
Membrane: polyvinylidene difluoride (PVDF)
Filter paper thickness: 0.8 mm
Binding capacity: 50–150 µg/cm2 for large, globular proteins; >150 µg/cm2 for smaller peptides
Re-probe characteristics: yes
Pre-activation: required with 100% alcohol (methanol, ethanol, or isopropanol)
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
Durability: compatible with most organic solvents, acids, and mild bases

Nylon membranes, 0.45 µm pore size, 8 x 7.3 cm (Invitrogen™)

Invitrogen makes blotting easier by providing a variety of pre-cut, pre-assembled membrane/filter-paper sandwiches. A protein's properties (i.e., charge, hydrophobicity, etc.) affect its ability to bind to membrane surfaces. Finding the right membrane may require experimenting with your specific protein on different membranes. Store all membranes at room temperature.

Power Blotter Pre-cut Membranes and Filters, PVDF, regular size (Invitrogen™)

These Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for the transfer of proteins from two mini-size or one midi-size gel using the Power Blotter System. The pre-cut membranes and filters include 20 sheets of polyvinylidene difluoride (PVDF) and 80 sheets of absorbent blotting paper (0.85 mm thickness). Power Blotter Pre-cut Membranes and Filters combined with Power Blotter 1-Step Transfer Buffer allow for high-efficiency semi-dry transfer of proteins in less than 10 minutes.

Features of PVDF membranes:
High-quality—PVDF transfer membranes manufactured especially for protein transfer and western blot applications
High-binding affinity—provides excellent protein binding (240 µg/cm2), blocks easily, and is compatible with most organic solvents, acids, and mild bases
Convenient—pre-cut PVDF sheets are ready to use for transfer and blotting experiments from two standard precast and homemade mini-gels or one midi-size gel (8 cm x 13 cm); no need to cut your own membranes, minimizing damage and contamination
Validated—membrane and filter when combined provide the thickness and buffer capacity needed for optimal performance in the Power Blotter System

The PVDF membrane (0.45 µm pore size, low fluorescence) has higher binding capacity than nitrocellulose. The membrane is compatible with commonly used detection methods such as staining, immunodetection, and fluorescence. The proteins bind to the membrane through hydrophobic interactions. This membrane has lower background levels and increased sensitivity for fluorescent probing than regular PVDF or nitrocellulose membranes.

Learn more about the Power Blotter System ›

Related products
Power Blotter Pre-cut Membranes and Filters, PVDF, mini
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, mini
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size

Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 µm, 8.3 x 7.3 cm (for mini gels) (Invitrogen™)

Invitrolon PVDF/Filter Paper Sandwiches, 0.45 µm, have excellent binding properties for western blotting, dot-blot assays, and protein sequencing. The 0.45-µm pore size makes these membranes ideal for protein transfer for proteins >10kDa.

See all membranes and filter papers ›

Features
Membrane: polyvinylidene difluoride (PVDF)
Filter paper thickness: 0.8 mm
Binding capacity: goat IgG: 294 µg/cm2; BSA: 131 µg/cm2; insulin: 85 µg/cm2
Re-probe characteristics: yes
Pre-activation: required with 100% alcohol (methanol, ethanol, or isopropanol)
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
Durability: compatible with most organic solvents, acids, and mild bases

Nitrocellulose Membrane, 0.45 µm, 30 cm x 3.5 m (Thermo Scientific™)

Pierce Nitrocellulose Membrane, 0.45 µm, comes in a convenient 30 cm x 3.5m roll and has excellent binding properties for western blotting, dot-blot assays, and other protein or nucleic acid methods. The 0.45-µm pore size is ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).

See all membranes ›

Features:
Membrane: 100% pure nitrocellulose
Binding capacity: ~80 µg/cm2 of proteins
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• Economical—30 cm x 3.5 m roll for cutting to any dimensions

Nitrocellulose/Filter Paper Sandwiches, 0.45 µm, 8.5 x 13.5 cm (Invitrogen™)

Nitrocellulose/Filter Paper Sandwiches, 0.45 µm, are high-quality membranes ideal for blotting proteins and nucleic acids. The 0.45-µm pore size is ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp). The included extra-thick absorbent blotting paper (2.5 mm) accommodates blotting set-up using semi-dry transfer apparatuses.

See all membranes and filter papers ›

Features:
Membrane: 100% pure nitrocellulose
Filter paper thickness: 2.5 mm
Binding capacity: ~80 µg/cm2 of proteins
Binding interactions: hydrophobic and electrostatic
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• Supplied in a pre-cut, pre-assembled membrane/filter paper sandwich

Power Blotter Select Transfer Stacks, PVDF, mini (Invitrogen™)

The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. They are disposable, pre-assembled stacks that come with an integrated PVDF transfer membrane for dry blotting of proteins from polyacrylamide gels. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins.

Features of the Power Blotter Select Transfer Stacks:
Rapid transfer—the ready-to-use transfer stack takes little setup time and protein transfers take place in as little as seven minutes
Efficient and even transfer—stacks are made with high-quality membranes that provide high binding efficiency, uniform transfer, and high detection sensitivity
Increased reproducibility—preassembled stacks provide consistent performance

Learn more about the use of the Power Blotter Select Transfer Stacks in Power Blotter System

PVDF membrane
The PVDF membrane (0.2 µm pore size, low fluorescence) in the stack has higher binding capacity than nitrocellulose. It is pre-activated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane through hydrophobic interactions. The protein-binding capacity is 240 µg/cm2.

Mini transfer stacks
The mini-size (8 cm x 8 cm) Power Blotter Select Stacks can be used with all Invitrogen Novex, Bolt, and NuPAGE mini-size gels or equivalent.

Related products
Power Blotter Select Transfer Stacks, PVDF, regular size
Power Blotter Select Transfer Stacks, nitrocellulose, mini size
Power Blotter Select Transfer Stacks, nitrocellulose, regular size

Power Blotter Select Transfer Stacks, nitrocellulose, mini (Invitrogen™)

The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. They are disposable, pre-assembled stacks that come with an integrated nitrocellulose transfer membrane for the dry blotting of proteins from polyacrylamide gels. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins.

Features of the Power Blotter Select Transfer Stacks:
Rapid transfer—the ready-to-use transfer stack takes little setup time with protein transfer taking place in as little as seven minutes
Efficient and even transfer—stacks are made with high-quality membranes that provide high binding efficiency, uniform transfer, and high detection sensitivity
Increased reproducibility—preassembled stacks provide consistent performance

Learn more about the use of the Power Blotter Select Transfer Stacks in Power Blotter System

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) in the stacks is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, chemiluminescence, and fluorescence. The proteins bind to the membrane through hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Mini transfer stacks
The mini-size (8 cm x 8 cm) Power Blotter Select Stacks can be used with all Invitrogen Novex, Bolt, and NuPAGE mini-size gels or equivalent.

Related products
Power Blotter Select Transfer Stacks, nitrocellulose, regular size
Power Blotter Select Transfer Stacks, PVDF, mini
Power Blotter Select Transfer Stacks, PVDF, regular size

Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 µm, 8.5 x 13.5 cm (Invitrogen™)

Invitrolon PVDF/Filter Paper Sandwiches, 0.45 µm, have excellent binding properties for western blotting, dot-blot assays, and protein sequencing. The 0.45-µm pore size makes these membranes ideal for protein transfer for proteins >10 kDa. The included extra-thick absorbent blotting paper (2.5 mm) accommodates blotting set-up using semi-dry transfer apparatuses.

See all membranes and filter papers ›

Features
Membrane: polyvinylidene difluoride (PVDF)
Filter paper thickness: 2.5 mm
Binding capacity: goat IgG: 294 µg/cm2; BSA: 131 µg/cm2; insulin: 85 µg/cm2
Re-probe characteristics: yes
Pre-activation: required with 100% alcohol (methanol)
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
Durability: compatible with most organic solvents, acids, and mild bases

Power Blotter Select Transfer Stacks, nitrocellulose, regular size (Invitrogen™)

The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. They are disposable, pre-assembled stacks that come with an integrated nitrocellulose transfer membrane for the dry blotting of proteins from polyacrylamide gels. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins.

Features of the Power Blotter Select Transfer Stacks:
Rapid transfer—the ready-to-use transfer stack takes little setup time and protein transfers take place in as little as seven minutes
Efficient and even transfer—stacks are made with high quality membranes that provide high binding efficiency, uniform transfer, and high detection sensitivity
Increased reproducibility—preassembled stacks provide consistent performance

Learn more about the use of the Power Blotter Select Transfer Stacks in Power Blotter System

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) in the stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, chemiluminescence, and fluorescence. The proteins bind to the membrane through hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Regular-size transfer stacks
Regular-size Power Blotter Select Stacks (13 cm x 8.3 cm) can be used with all Invitrogen Novex and NuPAGE midi-gels (or equivalent) or two mini-size gels (all Novex, Bolt, and NuPAGE mini-size gels, or equivalent).

Related products
Power Blotter Select Transfer Stacks, nitrocellulose, mini size
Power Blotter Select Transfer Stacks, PVDF, mini
Power Blotter Select Transfer Stacks, PVDF, regular size

ProBlott™ Membrane, mini PK/10 (protein sequencing accessory) (Applied Biosystems™)

This is a protein sequencing accessory.

For Research Use Only. Not for use in diagnostics procedures.

Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size (Invitrogen™)

These Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for transfer of proteins from two mini-size or one midi-size gel using the Power Blotter System. The pre-cut membranes and filters include 20 sheets of nitrocellulose and 80 sheets of absorbent blotting paper (0.85 mm thickness). Power Blotter Pre-cut Membranes and Filters combined with Power Blotter 1-Step Transfer Buffer allow for high-efficiency semi-dry transfer of proteins in less than 10 minutes.

Features of nitrocellulose membranes:
High-quality—pure 100% nitrocellulose membranes with high surface area and excellent uniformity
High-binding affinity—provides excellent protein binding (209 µg/cm2), blocks easily, and produces very low background in chemiluminescent and fluorescent western blotting
Convenient—pre-cut nitrocellulose sheets are ready to use for transfer and blotting experiments from two standard precast or homemade mini gels or one midi gel (8 cm x 13 cm); no need to cut your own membranes, minimizing damage and contamination
Validated—membrane and filter when combined provide the thickness and buffer capacity needed for optimal performance in the Power Blotter System

The nitrocellulose membrane (0.2 µM pore size) is composed of 100% pure nitrocellulose to provide high-quality transfers. Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (e.g., chemiluminescence, chromogenic, fluorescence). The pre-cut membrane/filter paper sandwiches fit two mini-size or one midi-size gel.

Learn more about the Power Blotter System ›

Related products
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, mini
Power Blotter Pre-cut Membranes and Filters, PVDF, mini
Power Blotter Pre-cut Membranes and Filters, PVDF, regular size

Western Blotting Filter Papers, 2.5-mm thick, 8.5 x 9 cm (Thermo Scientific™)

Pierce Western Blotting Filter Papers, 2.5-mm thick, are pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes.

See all filter papers ›

Pierce Western Blotting Filter Papers are composed of high-quality alpha cotton cellulose fiber for use in northern, southern, and western blotting applications. These filter papers help to ensure quick and efficient transfer of molecules from gel to membrane. They are manufactured with ultrapure water without additives to minimize possible sources of background signal. They are suitable for use with alcohol or other organic solvents commonly used in protein and nucleic acid blotting applications.

iBlot™ 2 Transfer Stacks, nitrocellulose, regular size (Invitrogen™)

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacks with an integrated nitrocellulose transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This regular-size stack (13 cm x 8.3 cm) can be used with midi-size gels or two mini-size gels or E-PAGE 48 or 96 gels.

Learn more about the iBlot 2 system ›

Nitrocellulose membrane
The nitrocellulose membrane (0.2-µm pore size) in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein-binding capacity is 209 µg/cm2.