Shop All Blotting Membranes

Power Blotter Select Transfer Stacks, nitrocellulose, mini (Invitrogen™)

The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. They are disposable, pre-assembled stacks that come with an integrated nitrocellulose transfer membrane for the dry blotting of proteins from polyacrylamide gels. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins.

Features of the Power Blotter Select Transfer Stacks:
Rapid transfer—the ready-to-use transfer stack takes little setup time with protein transfer taking place in as little as seven minutes
Efficient and even transfer—stacks are made with high-quality membranes that provide high binding efficiency, uniform transfer, and high detection sensitivity
Increased reproducibility—preassembled stacks provide consistent performance

Learn more about the use of the Power Blotter Select Transfer Stacks in Power Blotter System

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) in the stacks is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, chemiluminescence, and fluorescence. The proteins bind to the membrane through hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Mini transfer stacks
The mini-size (8 cm x 8 cm) Power Blotter Select Stacks can be used with all Invitrogen Novex, Bolt, and NuPAGE mini-size gels or equivalent.

Related products
Power Blotter Select Transfer Stacks, nitrocellulose, regular size
Power Blotter Select Transfer Stacks, PVDF, mini
Power Blotter Select Transfer Stacks, PVDF, regular size

Blotting Filter Papers, 2.5-mm thick, 8.6 x 13.5 cm (Invitrogen™)

Blotting Filter Papers, 2.5-mm thick, are pre-cut, extra-thick, absorbent blotting papers for use in semi-dry transfers. The 8.6 cm x 13.5-cm size accommodates one midi-size gel, one E-PAGE gel, or two mini-size gels.

See all filter papers ›

BrightStar™-Plus Positively Charged Nylon Membrane, 30 cm x 45 cm (Invitrogen™)

Ambion® BrightStar®-Plus Positively Charged Nylon Membrane(s) are strongly recommended to ensure consistent, reliable results with minimal background. Ideal for use with radiolabeled and nonisotopic probes for northern and other blot applications; one roll of 30 x 45 cm is provided. This membrane exhibits the lowest nonspecific binding in nonisotopic detection applications when used with Ambion® blocking and wash solutions.

Power Blotter Select Transfer Stacks, PVDF, regular size (Invitrogen™)

The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. They are disposable, pre-assembled stacks that come with an integrated PVDF transfer membrane for dry blotting of proteins from polyacrylamide gels. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins.

Features of the Power Blotter Select Transfer Stacks:
Rapid transfer—the ready-to-use transfer stack takes little setup time and protein transfers take place in as little as seven minutes
Efficient and even transfer—stacks are made with high-quality membranes that provide high binding efficiency, uniform transfer, and high detection sensitivity
Increased reproducibility—preassembled stacks provide consistent performance

Learn more about the use of the Power Blotter Select Transfer Stacks in Power Blotter System

PVDF membrane
The PVDF membrane (0.2 µm pore size, low fluorescence) in the stack has higher binding capacity than nitrocellulose. The PVDF membrane is pre-activated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane through hydrophobic interactions. The protein-binding capacity is 240 µg/cm2.

Regular-size transfer stacks
Regular-size Power Blotter Select Stacks (13 cm x 8.3 cm) can be used with all Invitrogen Novex and NuPAGE midi-gels (or equivalent) or two mini-size gels (all Novex, Bolt, and NuPAGE mini-size gels, or equivalent).

Related products
Power Blotter Select Transfer Stacks, PVDF, mini
Power Blotter Select Transfer Stacks, nitrocellulose, mini
Power Blotter Select Transfer Stacks, nitrocellulose, regular size

Nitrocellulose/Filter Paper Sandwiches, 0.2 µm, 8.5 x 13.5 cm (Invitrogen™)

Nitrocellulose/Filter Paper Sandwiches, 0.2 µm, are high-quality membranes ideal for blotting proteins and nucleic acids. The 0.2-µm pore size is ideal for the transfer of low molecular weight proteins (<20 kDa) or peptides and nucleic acids (<300 bp). The included extra-thick, absorbent blotting paper (2.5 mm) accommodates blotting set-up using semi-dry transfer apparatuses.

See all membranes and filter papers ›

Features:
Membrane: 100% pure nitrocellulose
Filter paper thickness: 2.5 mm
Binding capacity: ~80 µg/cm2 of proteins
Binding interactions: hydrophobic and electrostatic
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• Supplied in a pre-cut, pre-assembled membrane/filter paper sandwich

BrightStar™-Plus Positively Charged Nylon Membrane, 30 cm x 3 m (Invitrogen™)

Ambion® BrightStar®-Plus Positively Charged Nylon Membrane(s) are strongly recommended to ensure consistent, reliable results with minimal background. Ideal for use with radiolabeled and nonisotopic probes for northern and other blot applications; one roll of 30 cm x 3 m is provided. This membrane exhibits the lowest nonspecific binding in nonisotopic detection applications when used with Ambion® blocking and wash solutions.

Western Blotting Filter Papers, 2.5-mm thick, 7 x 8.4 cm (Thermo Scientific™)

Pierce Western Blotting Filter Papers, 2.5-mm thick, are pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes. The 7 x 8.4-cm size accommodates most mini-size hand-cast or pre-cast gels.

See all filter papers ›

Pierce Western Blotting Filter Papers are composed of high-quality alpha cotton cellulose fiber for use in northern, southern, and western blotting applications. These filter papers help to ensure quick and efficient transfer of molecules from gel to membrane. They are manufactured with ultrapure water without additives to minimize possible sources of background signal. They are suitable for use with alcohol or other organic solvents commonly used in protein and nucleic acid blotting applications.

iBlot™ Transfer Stack, nitrocellulose, regular size (Invitrogen™)

The iBlot® Transfer Stacks are used to transfer proteins using the iBlot® Gel Transfer Device. The iBlot® Transfer Stacks are disposable stacks that come in three varieties for different applications: iBlot® Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins, iBlot® DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA, and iBlot® Western Detection Stacks are used to perform Western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot® Transfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex® midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex® mini-gels (or equivalent).

Power Blotter Select Transfer Stacks, nitrocellulose, regular size (Invitrogen™)

The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. They are disposable, pre-assembled stacks that come with an integrated nitrocellulose transfer membrane for the dry blotting of proteins from polyacrylamide gels. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins.

Features of the Power Blotter Select Transfer Stacks:
Rapid transfer—the ready-to-use transfer stack takes little setup time and protein transfers take place in as little as seven minutes
Efficient and even transfer—stacks are made with high quality membranes that provide high binding efficiency, uniform transfer, and high detection sensitivity
Increased reproducibility—preassembled stacks provide consistent performance

Learn more about the use of the Power Blotter Select Transfer Stacks in Power Blotter System

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) in the stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, chemiluminescence, and fluorescence. The proteins bind to the membrane through hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Regular-size transfer stacks
Regular-size Power Blotter Select Stacks (13 cm x 8.3 cm) can be used with all Invitrogen Novex and NuPAGE midi-gels (or equivalent) or two mini-size gels (all Novex, Bolt, and NuPAGE mini-size gels, or equivalent).

Related products
Power Blotter Select Transfer Stacks, nitrocellulose, mini size
Power Blotter Select Transfer Stacks, PVDF, mini
Power Blotter Select Transfer Stacks, PVDF, regular size

Nitrocellulose Membrane, 0.2 µm, 7.9 cm x 10.5 cm (Thermo Scientific™)

These Thermo Scientific Pierce Nitrocellulose Membranes are 0.2 µM, 7.9 cm x 10.5 cm sheets of nitrocellulose that have excellent binding properties for western blotting, dot-blot assays and other protein or nucleic acid methods.

Features of Nitrocellulose Membranes:

High-quality—pure, 100% nitrocellulose membranes with high surface area and excellent uniformity
Peptide applications—0.2 µM pore size
Convenient—7.9 cm x 10.5 cm pre-cut sheets
High-binding affinity—provides excellent protein binding, blocks easily, and produces very low background in chemiluminescent Western blotting

Nitrocellulose is a popular binding matrix for Western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (e.g., chemiluminescence, chromogenic, fluorescence).

Related Products
Nitrocellulose Membrane, 0.2 µm, 8 cm x 12 cm
Nitrocellulose Membrane, 0.2 µm, 8 cm x 8 cm

Blotting Filter Papers, 2.5-mm thick, 7.5 x 8.4 cm (Invitrogen™)

Blotting Filter Papers, 2.5-mm thick, are pre-cut, extra-thick, absorbent blotting paper for use in semi-dry transfers. The 7.5-cm x 8.4-cm size accommodates most mini-size hand-cast or pre-cast gels.

See all filter papers ›

Nitrocellulose Membranes, 0.45 µm, 8 x 12 cm (Thermo Scientific™)

Pierce Nitrocellulose Membranes, 0.45 µm, 8 x 12 cm, have excellent binding properties for western blotting, dot-blot assays,and other protein or nucleic acid methods. The 0.45-µm pore size is ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).

See all membranes ›

Features:
Membrane: 100% pure nitrocellulose
Binding capacity: ~80 µg/cm2 of proteins
Compatibility: compatible with commonly used transfer conditions and detection methods, such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling

Western Blotting Filter Papers, 2.5-mm thick, 8.5 x 9 cm (Thermo Scientific™)

Pierce Western Blotting Filter Papers, 2.5-mm thick, are pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes.

See all filter papers ›

Pierce Western Blotting Filter Papers are composed of high-quality alpha cotton cellulose fiber for use in northern, southern, and western blotting applications. These filter papers help to ensure quick and efficient transfer of molecules from gel to membrane. They are manufactured with ultrapure water without additives to minimize possible sources of background signal. They are suitable for use with alcohol or other organic solvents commonly used in protein and nucleic acid blotting applications.

ProBlott™ Membrane, mini PK/10 (protein sequencing accessory) (Applied Biosystems™)

This is a protein sequencing accessory.

For Research Use Only. Not for use in diagnostics procedures.

iBlot™ Transfer Stack, nitrocellulose, mini (Invitrogen™)

iBlot® Transfer Stacks are used to transfer proteins using the iBlot® Gel Transfer Device. The iBlot® Transfer Stacks are disposable stacks that come in three varieties for different applications. iBlot® Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins. iBlot® DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA. iBlot® Western Detection Stacks are used to perform western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot® Transfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. Proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex® midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex® mini-gels (or equivalent).