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    Human Leptin Recombinant Protein Invitrogen™

    Human Leptin Recombinant Protein for Ctrl

    STK38 (NDR) Recombinant Human Protein Thermo Scientific™

    STK38 (also known as NDR) or Nuclear Dbf2-related protein is a serine/threonine kinase in the subgroup of the AGC kinase family that is regulated by Mob proteins. Fas and TNFα receptor stimulation activates human STK38 by promoting phosphorylation at the hydrophobic motif. NDR acts as a novel pro-apoptotic kinase, and is a key member of the RASSF1A/MST1 signaling cascade. STK38 is crucial for the fidelity of mitotic chromosome alignment in mammalian cells. STK38 is incorporated into the HIV-1 particles and is cleaved by the HIV-1 protease. STK38-driven centrosome duplication requires Cdk2 activity, and Cdk2-induced centrosome amplification is affected upon reduction of STK38 activity. Full-length recombinant human STK38 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

    • Validated in LanthaScreen® Kinase Binding Assay
    • Protocols available for simple use and optimized performance
    • Multiple pack sizes available for convenient use

    Related Product:
    LanthaScreen® Eu Kinase Binding Assay

    Epidermal Growth Factor Recombinant Protein Invitrogen™

    Human EGF Recombinant Protein for Ctrl

    Qtracker™ 800 Cell Labeling Kit Invitrogen™

    The reagents in the Qtracker® 800 Cell Labeling Kit use a custom targeting peptide to deliver near-infrared-fluorescent Qdot® 800 nanocrystals into the cytoplasm of live cells. Qtracker® Cell Labeling Kits are designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals. Once inside the cells, Qtracker® labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in a population.

    Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

    Key Attributes:

    Qtracker® 800 label has Ex/Em (405-760/800) nm
    Designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals
    Provide intense, stable fluorescence that can be traced through several generations
    Available in eight colors—525 nm, 565 nm, 585 nm, 605 nm, 625 nm, 655 nm, 705 nm, or 800 nm emission
    Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

    The Qtracker® Cell Labeling Kits use a custom targeting peptide to deliver Qdot® nanocrystals into the cytoplasm of live cells. Cytoplasmic delivery by this mechanism is not mediated by a specific enzyme; therefore, no cell-type specificity has been observed. Delivery is typically accomplished in less than 1 hour.

    Qdot® nanocrystals delivered by the Qtracker® Cell Labeling Kits are compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity. Furthermore, autofluorescence commonly observed in cells or tissues can be avoided using Qtracker® 655, 705, or 800 Kits.

    Long-Lasting, Targeted Signal
    Using Qtracker® Cell Labeling Kits, you can observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes. Qtracker® labels are distributed in vesicles in the cytoplasm, and are inherited by daughter cells for at least six generations. Fluorescence from the Qtracker® labels can be seen up to a week after delivery in some cell lines. Long-term cellular retention makes Qtracker® Cell Labeling Kits ideal for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Qtracker® labels do not leak out of intact cells to be taken up by adjacent cells in the population.

    Monitor the Signal on Multiple Platforms
    Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

    Minimal Impact on Live Cells
    The cytotoxicity of the materials use in Qtracker® Cell Labeling Kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling Kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

    Useful in a Variety of Cell Tracing Studies
    Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker® labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

    KIT V560G Recombinant Human Protein Invitrogen™

    This KIT V560G recombinant human protein (544–end) was expressed in insect cells. KIT V560G KIT receptor tyrosine kinase is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. KIT V560G recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

    • Validated for use with the Z’-LYTE™ assay
    • Available in multiple pack sizes for convenient use
    • Also available in our SelectScreen Kinase Profiling Service

    Fibroblast Growth Factor basic Recombinant Protein Invitrogen™

    Human FGF basic Recombinant Protein for Ctrl

    Iodine-Water-Pyridine-Tetrahydrofuran Oxidizer Solution (0.02 M) Applied Biosystems™

    This Iodine⁄Water⁄Pyridine⁄Tetrahydrofuran (oxidizer) solution comes in a convenient large volume for ABI 394 Nucleic Acid synthesizers, resulting in extended hands-free DNA synthesis. With these larger bottles attached your synthesizer can produce up to 40 average primer-length oligonucleotides with no bottle changes, reducing attended operation time by 50%.

    • Reagent concentrations are within our strict specifications, guaranteeing consistent quality.
    • Our performance specification of at least 98% stepwise coupling efficiency is assured, increasing oligonucleotide synthesis yields of full-length product.
    • Reagent kits are available for your convenience, and save you time and money.

    Our high-performance reagents begin with raw materials that must conform to strict specifications. Once the reagents are manufactured and packaged, we perform extensive testing to assure quality. Reagent lifetime on the instrument is two weeks for tetrazole, and six weeks for all other reagents.

    For Research Use Only. Not for use in diagnostics procedures.

    Human BCL2A1 Synthetic Peptide Invitrogen™

    Human BCL2A1 Synthetic Peptide for BLOCK, Ctrl

    pUC19 DNA Thermo Scientific™

    Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.

    Highlights

    • Purified by chromatography using proprietary patented technology
    • More than 90% in the supercoiled form
    • Isolated from E.coli (dam+, dcm+)
    • For pUC18 DNA sequence, pUC19 DNA sequence, sequence analysis and map creation, see free online REviewer tool.

    Applications

    • Cloning
    • Sequencing of insert DNA, pUC18 DNA: Preparation of DNA molecular weight standards

    pUC18/19 plasmid contents and usage notes - pUC18/19 plasmids contain:
    • The pMB1 replicon rep responsible for the replication of plasmid (source – plasmid pBR322). The high copy number of pUC plasmids is a result of the lack of the rop gene and a single point mutation in the replicon rep of pMB1.
    • The bla gene, encoding beta-lactamase, confers resistance to ampicillin (source – plasmid pBR322). It differs from that of pBR322 by two point mutations
    • The region of E.coli lac operon containing a CAP protein binding site, promoter Plac, lac repressor binding site and the 5’-terminal part of the lacZ gene encoding the N-terminal fragment of beta-galactosidase (source – M13mp18/19). This fragment, whose synthesis can be induced by IPTG, is capable of intra-allelic (alpha) complementation with a defective form of beta-galactosidase encoded by the host (mutation delta(lacZ)M15). In the presence of IPTG, bacteria synthesize both fragments of the enzyme and form blue colonies on media with X-Gal. Insertion of DNA into the MCS located within the lacZ gene (codons 6-7 of lacZ are replaced by MCS) inactivates the N-terminal fragment of beta-galactosidase and abolishes alpha-complementation. Bacteria carrying recombinant plasmids therefore give rise to white colonies

    The map shows enzymes that cut pUC18 DNA once. Enzymes produced by Thermo Scientific are shown in orange. The coordinates refer to the position of the first nucleotide in each recognition sequence.

    The exact positions of the genetic elements are shown on the map (termination codons included). The bla gene nucleotides 2486-2418 (complementary strand) code for a signal peptide. The LacZ polypeptide corresponding to wt beta-galactosidase and essential for blue/white screening ends at nt position 236 (compl. strand). Another 30 codons in the same reading frame are derived from pBR322. The indicated rep region is sufficient to promote replication. DNA replication initiates at position 866 (± 1) and proceeds in the direction indicated. Plasmids carrying the pMB1 and ColE1 replicons are incompatible, but they are fully compatible with those carrying the p15A replicon (pACYC177, pACYC184). pMB1-derived plasmids can be amplified using chloramphenicol.

    Related Products
    pUC18 DNA
    pUC57 DNA

    KSR2 Recombinant Human Protein Invitrogen™

    This KSR2 recombinant human protein (554–end) was expressed in insect cells. KSR2 serine/threonine kinase or kinase suppressor of Ras 2 specifically regulates the activity of MEKK3 and COT but not other members of the MAP3K family. KSR interacts with a regulatory Raf molecule in cis to induce a conformational switch of MEK, facilitating MEK's phosphorylation by a separate catalytic Raf molecule in trans. KSR2 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

    • Validated for use with the Z’-LYTE™ assay
    • Available in multiple pack sizes for convenient use
    • Also available in our SelectScreen Kinase Profiling Service

    STK38L (NDR2) Recombinant Human Protein

    STK38L (also known as NDR2) or Nuclear Dbf2-related protein is a serine/threonine kinase in the subgroup of the AGC kinase family. STK38L act as novel pro-apoptotic kinase that is activated by RASSF1A/MST1 in response to Fas receptor stimulation and promotes apoptosis. STK38L is mainly involved in the regulation of structural processes in differentiating and mature neuronal cells and it is highly expressed with highest levels observed in the thymus. Recombinant full-length human STK38L was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

    • Validated in LanthaScreen® Kinase Binding Assay
    • Protocols available for simple use and optimized performance
    • Multiple pack sizes available for convenient use

    Related Product:
    LanthaScreen® Eu Kinase Binding Assay

    Human Insulin Recombinant Protein Invitrogen™

    Human Insulin Recombinant Protein for Ctrl

    mMESSAGE mMACHINE™ T3 Transcription Kit Invitrogen™

    Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions.
    • High yields of capped RNA—up to 39 µg of RNA per reaction
    • Extra convenience—cap analog-nucleotide mix provided
    • Simplified reaction format
    • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped RNA Transcription will yield up to 35 µg of capped RNA. This is 10–50 times the yield obtained with conventional in vitro transcription reactions. The ratio of cap analog to GTP has been optimized to allow the best compromise between yield (15–35 µg) and proportion of transcripts that are capped (80%). The mMESSAGE mMACHINE® kits also contain a LiCl precipitation solution that is efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation. mMESSAGE mMACHINE® Kits are only optimized for use with the polymerases included in the kit. Using a different polymerase than what is supplied in the kit may result in low yields. Each mMESSAGE mMACHINE® Kit contains 57 U of cap analog, an outstanding value for the cost.

    ABL1 [Q252H] Recombinant Human Protein Thermo Scientific™

    Human ABL1, internal fragment, amino acids P118-S535 (as in GenBank entry NM_005157.3), Q252H mutant, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells.

    • Validated in LanthaScreen® Kinase Binding Assay
    • Protocols available for simple use and optimized performance
    • Multiple pack sizes available for convenient use

    Related Product:
    LanthaScreen® Eu Kinase Binding Assay

    FLT4 (VEGFR3) Recombinant Human Protein Thermo Scientific™

    FLT4 (VEGFR3), a receptor tyrosine kinase, is a key regulator of embryonic blood vessel development and adult angiogenesis. This kinase has been implicated in juvenile hemangioma, kaposi sarcoma, lymphangiomas, and hereditary lymphedemia.
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