This phospholipid analog of phosphocholine, ß-py-C10-HPC is labeled with pyrene on the sn-2 acyl chain. Pyrene forms excimers at high concentrations, excimer emission ∼470nm. Pyrene is environment sensitive, essentially nonfluorescent in water.
Oregon Green™ 488 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (Oregon Green™ 488 DHPE) Invitrogen™
The phospholipid, Oregon Green® 488 DHPE is labeled on the head group with the green-fluorescent Oregon Green® fluorophore with excitation/emission maxima ∼501/526 nm. This fluorinated analog of fluorescein overcomes some of the key limitations of fluorescein, including greater photostability and a...
Fluorescein DHPE (N-(Fluorescein-5-Thiocarbamoyl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) Invitrogen™
The phospholipid, fluorescein DHPE is labeled on the head group with the green-fluorescent pH-sensitive fluorophore, fluorescein (excitation/emission maxima ∼496/519 nm).
The phospholipid, Marina Blue® DHPE is labeled on the head group with the blue-fluorescent Marina Blue® fluorophore with excitation/emission maxima ∼365/460 nm.
Lissamine™ Rhodamine B 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt (rhodamine DHPE) Invitrogen™
The phospholipid, rhodamine DHPE is labeled on the head group with the red-fluorescent rhodamine B fluorophore with excitation/emission maxima ∼560/580 nm.
Bis-BODIPY™ FL C11-PC) (1,2-Bis-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Undecanoyl)-sn-Glycero-3-Phosphocholine) Invitrogen™
Molecular Probes® phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2.
β-BODIPY™ FL C12-HPC (2-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoyl)-1-Hexadecanoyl-sn-Glycero-3-Phosphocholine) Invitrogen™
The intensely fluorescent BODIPY® (4,4-difluoro-3a,4a-diaza-s-indacene) fluorophore is intrinsically lipophilic, unlikemost other long-wavelength dyes. Consequently, probes incorporating this fluorophore are more likely to mimic the properties of natural lipids.
BODIPY™ FL DHPE (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionyl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) Invitrogen™
BODIPY® DHPE has the green-fluorescent dye attached to the head group of the phospholipid. BODIPY® FL can form excimers at high concentrations and unlike fluorophores like pyrene, DPH and NBD, BODIPY® FL is relatively environment insensitive and is fluorescent in both aqueous and lipid environments.
NBD-PE (N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) Invitrogen™
The phospholipid, NBD-PE is labeled on the head group with the environment sensitive fluorophore, NBD (excitation/emission maxima ∼463/536 nm).
Texas Red™ 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt (Texas Red™ DHPE) Invitrogen™
The phospholipid, Texas Red™ 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Texas Red™ DHPE) is labeled on the head group with the bright, red-fluorescent Texas Red™ dye (excitation/emission maxima ∼595/615 nm).