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Orion™ pH Buffer Bottles Thermo Scientific™

Perform accurate, reproducible pH calibrations with color-coded Thermo Scientific™ Orion™ pH Buffer Bottles. pH 4.01, 5.00, 7.00 and 10.01 buffers are color coded for ease of use. All Orion pH buffers are traceable to NIST™ standard reference materials.

BupH™ Borate Buffer Packs Thermo Scientific™

Thermo Scientific BupH Borate Buffer Packs are pouches of dry-blended powder that are each sufficient to make 500 mL of borate buffer for use in protein modification procedures requiring amine-free conditions and alkaline pH.

Features of BupH Borate Buffer Packs:

Borate buffer—dissolved in 500 mL of water, each pack makes 50 mM borate, pH 8.5
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of borate buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 50 mM borate, pH 8.5.

Applications:
• Protein modification procedures requiring amine-free buffer at alkaline pH

Related Products
Pierce™ 20X Borate Buffer

Orion™ Total Alkalinity Test Kit Thermo Scientific™

Save time by eliminating additional equipment set-up, titrations and calculations. The Thermo Scientific™ Orion™ Total Alkalinity Test Kit provides two-step direct measurement of total alkalinity and pH using a pH electrode.

BupH™ MES Buffered Saline Packs Thermo Scientific™

Thermo Scientific BupH MES Buffered Saline Packs are pouches of dry-blended powder that are each sufficient to make 500 mL of optimized amine-free and carboxyl-free buffer for carbodiimide (EDC) crosslinking and conjugation reactions.

Features of BupH MES Buffered Saline Packs:

MES buffer—dissolved in 500 mL of water, each pack makes 0.1M MES, 0.9% sodium chloride, pH 4.7
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of MES buffered saline are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M MES, 0.9% sodium chloride, pH 4.7. MES is 2-(N-morpholino) ethanesulfonic acid.

Applications:
• Carboxyl-to-amine crosslinking procedures using carbodiimide crosslinker EDC (Part No. 22980)

BupH™ Modified Dulbecco's PBS Packs Thermo Scientific™

Thermo Scientific BupH Modified Dulbecco's PBS Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of physiological Na- and K-phosphate buffered saline (D-PBS) for ELISA and Western blot diluents and wash buffers.

Features of BupH Modified Dulbecco's PBS Packs:

Dulbecco's PBS—dissolved in 500 mL of water, each pack makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, pH 7.4
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of modified Dulbecco's phosphate buffered saline are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, pH 7.4.

Applications:
• Wash buffers and antibody diluents in applications such as ELISA, Western blotting and other immunoassays

Related Products
Pierce™ 20X Modified Dulbecco's PBS Buffer

Orion™ pH Buffer Individual Use Pouches Thermo Scientific™

Ensure fresh, ready to use standards for pH calibration with Thermo Scientific™ Orion™ pH Buffer Individual Use Pouches. Buffers are color coded for ease of use. All Orion pH buffers are traceable to NIST™ standard reference materials.

 

Orion™ ROSS™ All-in-One™ pH Buffer Kit Thermo Scientific™

Maintain pH electrodes more effectively with Thermo Scientific™ Orion™ ROSS™ All-in-One™ pH Buffer Kits. Convenient buffer kit includes 4.01, 7.00 and 10.01 buffers, ROSS storage solution, cleaning solution and storage bottle.

BupH™ Tris Buffered Saline Packs Thermo Scientific™

Thermo Scientific BupH Tris Buffered Saline Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of Tris-NaCl buffer (TBS) for diluents and wash buffers in ELISA, Western blotting and general immunoassay procedures.

Features of Thermo Scientific BupH Tris Buffered Saline Packs:

TBS—dissolved in 500 mL of water, each pack makes 25 mM Tris, 0.15M NaCl, pH 7.2
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Wash buffer and antibody diluent for ELISA, Western blotting and other immunoassays

BupH Dry-Blend Packs of TBS are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 25 mM Tris, 0.15M NaCl, pH 7.2.

Related Products
Pierce™ 20X TBS Buffer

Pierce™ 10X Western Blot Transfer Buffer, Methanol-free Thermo Scientific™

Thermo Scientific Pierce 10X Western Blot Transfer Buffer, Methanol-free is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus.

Features of 10X Western Blot Transfer Buffer, Methanol-free:

Transfer Buffer—diluted 10-fold in water, the solution is ready to use for electrophoresis (i.e., wet tank transfer from mini gels)
Easy to use—no packets to open, no powder to dissolve, and no methanol required
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—10X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 10X Western Blot Transfer Buffer is ready to use upon dilution to 1X with water; it does not contain methanol and the addition of methanol is not necessary for use in electrophoresis.

Applications:
• Wet (tank) western blot transfer of proteins to a membrane

Orion™ Pure Water™ pH Buffers and pHISA™ Adjustor Thermo Scientific™

Sample well water, rain water, distilled/deionized water, boiler feed water, process water and more with the Thermo Scientific™ Orion™ Pure Water™ pH Buffers and pHISA™ Adjustor. It offers fast, accurate pH results in high purity and low ionic strength waters.

Pierce™ 20X Modified Dulbecco's PBS Tween™ 20 Buffer Thermo Scientific™

Thermo Scientific Pierce 20X Modified Dulbecco's PBS Tween-20 is a space-saving stock solution used to prepare physiological Na- and K-phosphate buffered saline (D-PBS) wash and blocking buffers for ELISA, Western and other immunoassays.

Features of 20X Modified Dulbecco's PBS Tween-20:

Dulbecco's PBS-T—diluted 20-fold in water, the solution yields 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, 0.05% Tween-20, pH 7.4
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Modified Dulbecco's PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, 0.05% Tween™ 20, pH 7.4, when diluted to 1X with water.

Applications:
• A wash buffer for ELISA, Western blotting and other immunoassays
• Diluent or blocking buffer for plate-based assays

Related Products
Pierce™ 20X Modified Dulbecco's PBS Buffer

Pierce™ 10X Tris-Glycine SDS Buffer Thermo Scientific™

Thermo Scientific Pierce 10X Tris-Glycine-SDS Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine-SDS (pH 8.3) running buffer used for SDS-PAGE (protein polyacrylamide gel electrophoresis).

Features of 10X Tris-Glycine-SDS Buffer:

Tris-glycine-SDS buffer—diluted 10-fold in water, the solution yields 0.025M Tris, 0.192M glycine, 0.1% SDS, pH 8.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—10X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 10X Tris-Glycine-SDS Buffer makes 0.025M Tris, 0.192M glycine, 0.1% SDS, pH 8.5, when diluted to 1X with water. SDS is sodium dodecyl sulfate.

Applications:
• Running buffer for Tris-glycine gel electrophoresis

Related Products
BupH™ Tris-Glycine-SDS Buffer Packs

Anode Buffer Container (ABC) 3500 Series Applied Biosystems™

The anode buffer container (ABC) contains 1x running buffer to support all electrophoresis applications on the Applied Biosystems 3500 series Genetic Analyzers. It is made in a ready-to-use, disposable container with a radio frequency identification (RFID) tag incorporated into the label. The top of the ABC is heat sealed with a plastic film, which should be removed prior to direct installation on to the instrument. Each package includes four individual containers.

For Research Use Only. Not for use in diagnostics procedures.

Orion™ pH Buffer Cubitainers™ Thermo Scientific™

Hold 5 gallons (19L) of pH buffer in color-coded Thermo Scientific™ Orion™ pH Buffer Cubitainers™ with easy-pour spigot.

BupH™ Tris-HEPES-SDS Running Buffer Thermo Scientific™

Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of running buffer for gel electrophoresis (SDS-PAGE) with Thermo Scientific Precise Precast Protein Gels.

Features of Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs:

Tris-HEPES-SDS buffer—dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Running buffer for SDS-PAGE with Thermo Scientific Precise Precast Gels (e.g., Part No. 25224)

BupH Dry-Blend Packs of Tris-HEPES-SDS buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8. HEPES is 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.

Related Products
Pierce™ 20X Tris-HEPES-SDS Buffer
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